BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0476b.2
(264 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8257 similar to GB|AAL87403.1|19548077|AY081833 At2g20690/F5H1... 33 0.039
TC12036 similar to GB|AAP78935.1|32306503|BT009667 At4g01860 {Ar... 28 1.6
NP459520 RNA polymerase beta subunit [Lotus japonicus] 27 4.7
TC11175 weakly similar to GB|AAL06796.1|15809736|AY054135 AT3g60... 27 4.7
TC10122 27 4.7
AV424254 26 6.2
TC19447 weakly similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, ... 26 6.2
TC18348 similar to UP|BAD09200 (BAD09200) Short-chain dehydrogen... 26 6.2
BP038921 26 8.0
>TC8257 similar to GB|AAL87403.1|19548077|AY081833 At2g20690/F5H14.34
{Arabidopsis thaliana;}, partial (72%)
Length = 1404
Score = 33.5 bits (75), Expect = 0.039
Identities = 18/70 (25%), Positives = 35/70 (49%)
Frame = -1
Query: 109 RWKKVLRLLICNPVTSSWAPLRTRCGEYDANANVNIIIIPATAPTRNDYRLLSVTRQNKI 168
R ++ R+ ICN + + D + +++ P RND+ LLS++ Q++I
Sbjct: 915 RPQRASRICICNGLNKTTTEKPLHILSKDIHLKIHL---PPNLLQRNDHLLLSISHQHEI 745
Query: 169 FPPYVLKEYN 178
P+++K N
Sbjct: 744 EAPFIIKHIN 715
>TC12036 similar to GB|AAP78935.1|32306503|BT009667 At4g01860 {Arabidopsis
thaliana;}, partial (6%)
Length = 495
Score = 28.1 bits (61), Expect = 1.6
Identities = 20/72 (27%), Positives = 36/72 (49%)
Frame = -1
Query: 103 LIVVRLRWKKVLRLLICNPVTSSWAPLRTRCGEYDANANVNIIIIPATAPTRNDYRLLSV 162
+I+ + R KK I PV S + + +++N N+I++PAT+P+R + L
Sbjct: 309 IILEKERKKKFNNYFIFTPVISLVSGS*KVNYLHPSSSNCNLIMVPATSPSR*RFWLWHT 130
Query: 163 TRQNKIFPPYVL 174
Q +F + L
Sbjct: 129 NYQMCMFNKFTL 94
>NP459520 RNA polymerase beta subunit [Lotus japonicus]
Length = 3213
Score = 26.6 bits (57), Expect = 4.7
Identities = 8/25 (32%), Positives = 18/25 (72%)
Frame = -3
Query: 194 GDRKLNISKPAIVNEWIYFMSDNYH 218
G ++++ + + NE++YF +D+YH
Sbjct: 985 GPIQIDLVRDLLPNEYVYFSNDSYH 911
>TC11175 weakly similar to GB|AAL06796.1|15809736|AY054135
AT3g60690/T4C21_100 {Arabidopsis thaliana;}, partial
(42%)
Length = 692
Score = 26.6 bits (57), Expect = 4.7
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = -1
Query: 130 RTRCGEYDANANVNIIIIPATAPTRNDYRLLSVTRQNKIFPP 171
+T+ +AN ++IP A R R SVTRQ + PP
Sbjct: 407 QTKKSPQRVHANPKRVLIPDPAAIRVLTRSNSVTRQGMVIPP 282
>TC10122
Length = 544
Score = 26.6 bits (57), Expect = 4.7
Identities = 19/48 (39%), Positives = 23/48 (47%)
Frame = -3
Query: 72 NLLEDGPAAGVPTDFFNVVARSGKILSSWNGLIVVRLRWKKVLRLLIC 119
NLL+DG AA V + VVA G W +VVR W+ L C
Sbjct: 404 NLLDDGGAALVGGERVGVVAGHGVGDGGWR--VVVR*VWRVWYHLCSC 267
>AV424254
Length = 416
Score = 26.2 bits (56), Expect = 6.2
Identities = 12/39 (30%), Positives = 19/39 (47%)
Frame = +2
Query: 116 LLICNPVTSSWAPLRTRCGEYDANANVNIIIIPATAPTR 154
LL+ + + S W P+RT+CG + P P+R
Sbjct: 35 LLLLHLLHSPWKPMRTKCGHCSSRTTTP----PTPTPSR 139
>TC19447 weakly similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partial
(22%)
Length = 534
Score = 26.2 bits (56), Expect = 6.2
Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = -3
Query: 100 WNGLIVVRLR---WKKVLRLLICNPVTSSWAPLRTRC 133
W G + R R W+++ R ++C ++S+ A R+RC
Sbjct: 112 WRGSRMFR*RRSFWRQIRRSVLCARISSASATPRSRC 2
>TC18348 similar to UP|BAD09200 (BAD09200) Short-chain
dehydrogenase/reductase family protein-like, partial
(12%)
Length = 611
Score = 26.2 bits (56), Expect = 6.2
Identities = 20/84 (23%), Positives = 35/84 (40%), Gaps = 9/84 (10%)
Frame = +1
Query: 183 QWQVLNPDMNLGDRKLNISKPAIVNEWIYFMSDNYHYMNFIDDATLMPYIM-------AY 235
QW D + L + + V +Y SD+Y + F +PY M ++
Sbjct: 319 QWLKAIGDCTFRTKTLGLRRWIFVPLLLYSTSDSYIILIFCLLFPYLPYFMSLNYNFVSF 498
Query: 236 SHTDH--TSVRLDLPNNALEYLFH 257
+H H ++ L+L N+ + L H
Sbjct: 499 NHFQH*CLTINLELINDVPDQLMH 570
>BP038921
Length = 579
Score = 25.8 bits (55), Expect = 8.0
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +1
Query: 98 SSWNGLIVVRLRWKKVLRLLICNPVTSSWAPLRTRCGE 135
SS GLI+V RW +R L+ ++ + +R CG+
Sbjct: 367 SSMYGLIIVHERWYI*IRTLVLWKSSTQLSNVRVLCGQ 480
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.140 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,420,994
Number of Sequences: 28460
Number of extensions: 80063
Number of successful extensions: 455
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 454
length of query: 264
length of database: 4,897,600
effective HSP length: 88
effective length of query: 176
effective length of database: 2,393,120
effective search space: 421189120
effective search space used: 421189120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0476b.2