BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0476b.10
(527 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI419994 141 2e-34
BP076687 121 3e-28
BP052653 117 6e-27
AV413010 96 2e-20
AV410359 72 3e-19
TC18730 similar to UP|Q963J2 (Q963J2) Voltage-dependent calcium ... 87 7e-18
CB826914 80 6e-16
AV412339 77 5e-15
TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromo... 74 8e-14
AI967453 72 3e-13
BG662160 69 2e-12
AV422333 68 3e-12
TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (... 67 6e-12
AV768207 65 2e-11
AV427033 65 3e-11
AI967342 45 7e-11
AV423520 63 1e-10
AW720409 60 1e-09
TC13380 59 2e-09
BP044058 59 3e-09
>BI419994
Length = 549
Score = 141 bits (356), Expect = 2e-34
Identities = 83/165 (50%), Positives = 104/165 (62%), Gaps = 14/165 (8%)
Frame = +2
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKN 69
LP ELI +ILLRLPVRSLLRFK VC SW +LISDPQF +SHF+L A+PTHR+ L +
Sbjct: 47 LPDELILQILLRLPVRSLLRFKTVCKSWRYLISDPQFTKSHFNLAASPTHRIFLNNTNGF 226
Query: 70 KLESLDLESS----SLFVTLNLP--PPCKSRDH-----NSLYFLGSCRGFMLLAYDYNRQ 118
+ESLD +SS S V L P PP L LGSCRGF+LLA ++
Sbjct: 227 *IESLDTDSSLHDESSLVHLKFPLAPPHPDNHRFGSSVTGLKVLGSCRGFVLLA-NHQGN 403
Query: 119 VIVWNPSTGFYKQILSFSDFM---LDSLYGFGYDNSTDDYFLVLI 160
V+VWNPSTG +++ + ++ + LYG GYD S DDY +V I
Sbjct: 404 VVVWNPSTGVRRRVPEWCSYLASQCEFLYGIGYDESNDDYLVVFI 538
>BP076687
Length = 389
Score = 121 bits (303), Expect = 3e-28
Identities = 66/129 (51%), Positives = 88/129 (68%), Gaps = 9/129 (6%)
Frame = -3
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKN 69
LP ELI +ILLRLPVRSLLRFK VC SW LISDP+F +SH+DL A+PT+R L D +
Sbjct: 381 LPEELIPQILLRLPVRSLLRFKSVCKSWLSLISDPKFGKSHYDLAASPTYRCLTIAND-S 205
Query: 70 KLESLDL-------ESSSLFVTLNLPPPCKSR--DHNSLYFLGSCRGFMLLAYDYNRQVI 120
++ S+D+ ESS + + LPPPCK N+++FLG CRGF+++ Y+ V
Sbjct: 204 EIGSIDVDAPLLNYESSVVKLKYPLPPPCKLSLFSGNTVWFLGYCRGFVIVVYEQG-DVF 28
Query: 121 VWNPSTGFY 129
+WNPSTG +
Sbjct: 27 LWNPSTGVH 1
>BP052653
Length = 552
Score = 117 bits (292), Expect = 6e-27
Identities = 68/125 (54%), Positives = 83/125 (66%), Gaps = 2/125 (1%)
Frame = +1
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKN 69
LPH+LI +ILLRLPVRSLLRFK VC SW LISDPQF +SHFDL AAPTHR L+ ++
Sbjct: 187 LPHDLIVQILLRLPVRSLLRFKSVCKSWLSLISDPQFGKSHFDLAAAPTHRCLVK-INNF 363
Query: 70 KLESLDLESSSLFVT--LNLPPPCKSRDHNSLYFLGSCRGFMLLAYDYNRQVIVWNPSTG 127
KLESLD+++S L + +NL P + LGSCRGF+ L IVWNPS
Sbjct: 364 KLESLDVDASVLDQSSEVNLKFPYTGYVIFHIQILGSCRGFIALV--RGGDAIVWNPSXC 537
Query: 128 FYKQI 132
++I
Sbjct: 538 VQRKI 552
>AV413010
Length = 416
Score = 95.5 bits (236), Expect = 2e-20
Identities = 56/114 (49%), Positives = 70/114 (61%), Gaps = 10/114 (8%)
Frame = +3
Query: 3 NEKTNPTLPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLL 62
N + LP ELI +ILL+LPV SLLRFK V SWF IS+PQFA+SHF+L A+PTH L
Sbjct: 75 NPSLSSILPDELIIQILLQLPVTSLLRFKSVSKSWFSQISNPQFAKSHFNLAASPTHYLF 254
Query: 63 LPCLDKNKLESLDLESSSLFVT--LNLPPPCKSRDHNSLY--------FLGSCR 106
L D+ + ++LD+ESS F T LN P NS++ LGSCR
Sbjct: 255 LKSTDEFQFQTLDIESSPPFTTAVLNYPHEQPRSGSNSIFCPSITDLEVLGSCR 416
>AV410359
Length = 342
Score = 72.4 bits (176), Expect(2) = 3e-19
Identities = 40/72 (55%), Positives = 49/72 (67%), Gaps = 6/72 (8%)
Frame = +2
Query: 25 RSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCL-DKNKLESLDL-----ES 78
RSL+RFK VC SW LISDP+F +SHFDL AAPTHR L+ + D +++ESLD +
Sbjct: 5 RSLIRFKSVCKSWRSLISDPKFGKSHFDLAAAPTHRYLVRKINDSSEIESLDFNASLHDE 184
Query: 79 SSLFVTLNLPPP 90
SS V L LP P
Sbjct: 185SSAIVKLTLPFP 220
Score = 39.7 bits (91), Expect(2) = 3e-19
Identities = 16/28 (57%), Positives = 22/28 (78%)
Frame = +1
Query: 97 NSLYFLGSCRGFMLLAYDYNRQVIVWNP 124
N +Y LGSCRGF+++AY+ V+VWNP
Sbjct: 262 NPIYILGSCRGFVVVAYE-RGSVVVWNP 342
>TC18730 similar to UP|Q963J2 (Q963J2) Voltage-dependent calcium channel
alpha13 subunit (Fragment), partial (5%)
Length = 515
Score = 87.0 bits (214), Expect = 7e-18
Identities = 45/73 (61%), Positives = 57/73 (77%)
Frame = +2
Query: 13 ELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKNKLE 72
ELI ILLRLPVRSLLRFK VC SW LISDP+F SH+D++A+PTHR L D +++E
Sbjct: 230 ELILHILLRLPVRSLLRFKSVCKSWLSLISDPKFGXSHYDISASPTHRCLTXGND-SEIE 406
Query: 73 SLDLESSSLFVTL 85
S+D+++SSL V L
Sbjct: 407 SIDVDASSLPVIL 445
>CB826914
Length = 575
Score = 80.5 bits (197), Expect = 6e-16
Identities = 53/141 (37%), Positives = 73/141 (51%), Gaps = 16/141 (11%)
Frame = +1
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKN 69
+ +LI EILL LPV+S++RFK VC W LISDP FA HF AAP+ L D +
Sbjct: 166 MDRDLIIEILLMLPVKSIVRFKAVCKLWRSLISDPLFASLHFQ-RAAPS----LLFADSH 330
Query: 70 KLESLDLESSSLFVTLNLPPPCK--------SRDHNSLYFLGSCRGFMLL--------AY 113
+ ++DLE ++ P C S N + GSCRGF+L+ +
Sbjct: 331 AIRTIDLEGPLQSHRVSQPINCHFLSNYHDLSLPRNCISIHGSCRGFLLITWISNIGYGH 510
Query: 114 DYNRQVIVWNPSTGFYKQILS 134
+N + +WNPST +K I S
Sbjct: 511 PWNDSLYLWNPSTHVHKPIPS 573
>AV412339
Length = 420
Score = 77.4 bits (189), Expect = 5e-15
Identities = 53/118 (44%), Positives = 68/118 (56%), Gaps = 11/118 (9%)
Frame = +3
Query: 56 APTHRLLLPCL-DKNKLESLDLESS----SLFVTLNLPPPCKSRDHNSLYFLGSCRGFML 110
+PTHRLLL + D +++ESLD+E+S S V L PP S LGSCRG L
Sbjct: 3 SPTHRLLLNRINDGSEMESLDVEASLDDPSAVVNLKFPP-------YSGRTLGSCRGLFL 161
Query: 111 LAYDYNRQ------VIVWNPSTGFYKQILSFSDFMLDSLYGFGYDNSTDDYFLVLIGL 162
L + + +IVWNPSTG Y++ + D +LYGFGYD S DDY LV+I L
Sbjct: 162 LTKEMSPSGSSPGDLIVWNPSTGAYREFPDW-DSGSHTLYGFGYDPSNDDYLLVVIRL 332
>TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromosome 5, TAC
clone:K14B20, partial (7%)
Length = 782
Score = 73.6 bits (179), Expect = 8e-14
Identities = 73/226 (32%), Positives = 104/226 (45%), Gaps = 24/226 (10%)
Frame = +3
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHF-----DLNAAPTHRLLL- 63
LP ELI EIL LPV+SLL+F+ V +W ISDPQF + H NA H LL
Sbjct: 48 LPDELIIEILSWLPVKSLLQFRVVSKTWKSFISDPQFVKLHLLHRLSFRNADFEHTSLLI 227
Query: 64 ---------PCLDKNKLESLDLESSSLFVTLNLPPPCKSRDHNSLY-FLGSCRGFMLLA- 112
P + + SL LES S V SR S Y F+G+C G + L
Sbjct: 228 KCHTDDFGRPYISSRTVSSL-LESPSAIVA--------SRSCISGYDFIGTCNGLVSLRK 380
Query: 113 --YDYNR-----QVIVWNPSTGFYKQILSFSDFMLDSLYGFGYDNSTDDYFLVLIGLIWV 165
YD + QV WNP+T Q S + GFGYD S+D Y ++G+I
Sbjct: 381 LNYDESNTNNFSQVRFWNPATRTMSQDSPPSWSPRNLHLGFGYDCSSDTY--KVVGMIPG 554
Query: 166 KAIIQAFSVKTNSCDFKYVNAQYRDLGYHYRHGVFLNNSLHWLVTT 211
++ +++ N ++ + + V+++N+L+WL T
Sbjct: 555 LTMVNVYNMGDNC--WRTIQISPHAPMHLQGSAVYVSNTLNWLAGT 686
>AI967453
Length = 456
Score = 71.6 bits (174), Expect = 3e-13
Identities = 59/155 (38%), Positives = 81/155 (52%), Gaps = 13/155 (8%)
Frame = +1
Query: 14 LIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKNKLES 73
LI EILLRLPV+SL+RFK VC W LISDP FA SHF+ AAP RLL+ + + +
Sbjct: 16 LITEILLRLPVKSLVRFKAVCKFWRSLISDPHFATSHFE-RAAP--RLLI--RTDHGIRT 180
Query: 74 LDLESS------SLFVTLNLP---PPCKSRDHNSLYFLGSCRGFMLLAYDYNRQVIVWNP 124
+DLE S S + + P P R S +L S +R + +WNP
Sbjct: 181 MDLEESLHPDRISELIDYDFPYTVPYIVVRVELSANWL*S-----------SRSLSIWNP 327
Query: 125 STGFYKQI-LSFSDFMLDSLYGFGY---DNSTDDY 155
ST +K + + + + D +Y + Y +S DDY
Sbjct: 328 STHVHKPLPVDANAYEYDHMYPYEYGLVTSSKDDY 432
>BG662160
Length = 445
Score = 68.9 bits (167), Expect = 2e-12
Identities = 54/158 (34%), Positives = 75/158 (47%), Gaps = 15/158 (9%)
Frame = +2
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKN 69
LP EL EIL LPV+SL+RF+CV SW +ISD QF + H +++ T D
Sbjct: 23 LPEELRLEILSWLPVKSLVRFRCVSKSWKSIISDSQFIKLHLHRSSSTTRN-----TDFA 187
Query: 70 KLESLDLESSSLFVTLNLPPPCKSRDHNSLY------FLGSCRGFMLL---AYD----YN 116
L+SL + P KSR +++ F G+C G + L YD Y
Sbjct: 188 YLQSL------------ITSPRKSRSASTIAIPDDYDFCGTCNGLVCLHSSKYDRKDKYT 331
Query: 117 RQVIVWNPSTGFYKQILS--FSDFMLDSLYGFGYDNST 152
V +WNP+ Q + + DS +GF YD+ST
Sbjct: 332 SHVRLWNPAMRSMSQRSPPLYLPIVYDSYFGFDYDSST 445
>AV422333
Length = 482
Score = 68.2 bits (165), Expect = 3e-12
Identities = 50/138 (36%), Positives = 65/138 (46%), Gaps = 33/138 (23%)
Frame = +2
Query: 58 THRLL---LPCLDKNKLESLDLESSS----LFVTLNLPPPCKSRDHNSLYF--LGSCRGF 108
THRLL D +++ES DL++S V L PP H+ Y LGSCRGF
Sbjct: 2 THRLLRERFNNFDISEIESFDLKASLHDDFAVVNLKFPP------HSGTYIKALGSCRGF 163
Query: 109 MLLAYDYNRQVIVWNPSTGFYKQILS------------------------FSDFMLDSLY 144
++L +I+WNPSTG +K+I + F+ D L
Sbjct: 164 IMLTSPKLGDLILWNPSTGAHKEIPATYVDWDSWYDPSTSTHVRRGIPPYFAGLFFDPLS 343
Query: 145 GFGYDNSTDDYFLVLIGL 162
GFGYD S DDY LV+I L
Sbjct: 344 GFGYDQSKDDYLLVIIRL 397
>TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (SLF)-S4
protein, partial (10%)
Length = 551
Score = 67.4 bits (163), Expect = 6e-12
Identities = 59/202 (29%), Positives = 89/202 (43%)
Frame = +3
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLDKN 69
LP ELI EIL LPV+SLL+F+ V +W ISDPQF + H HRL
Sbjct: 21 LPDELIIEILSWLPVKSLLQFRVVSKTWKSFISDPQFVKLHL------LHRL-------- 158
Query: 70 KLESLDLESSSLFVTLNLPPPCKSRDHNSLYFLGSCRGFMLLAYDYNRQVIVWNPSTGFY 129
+ D E +SL + C + D Y +R V WNP+T
Sbjct: 159 SFRNADFEHTSLLI------KCHTDDFGRPYI-------------SSRTVRFWNPATRTM 281
Query: 130 KQILSFSDFMLDSLYGFGYDNSTDDYFLVLIGLIWVKAIIQAFSVKTNSCDFKYVNAQYR 189
Q S + GFGYD S+D Y ++G+I ++ +++ N ++ +
Sbjct: 282 SQDSPPSWSPRNLHLGFGYDCSSDTY--KVVGMIPGLTMVNVYNMGDNC--WRTIQISPH 449
Query: 190 DLGYHYRHGVFLNNSLHWLVTT 211
+ V+++N+L+WL T
Sbjct: 450 APMHLQGSAVYVSNTLNWLAGT 515
>AV768207
Length = 374
Score = 65.5 bits (158), Expect = 2e-11
Identities = 33/58 (56%), Positives = 41/58 (69%), Gaps = 2/58 (3%)
Frame = +1
Query: 8 PTLPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAA--PTHRLLL 63
PTLP EL EIL RLPV+SLL+ +CVC SW LISDP+FA++H ++ HRL L
Sbjct: 67 PTLPFELGVEILCRLPVKSLLQLRCVCKSWKSLISDPKFAKNHLRCSSTDFTRHRLFL 240
>AV427033
Length = 420
Score = 65.1 bits (157), Expect = 3e-11
Identities = 34/75 (45%), Positives = 47/75 (62%)
Frame = +1
Query: 8 PTLPHELIAEILLRLPVRSLLRFKCVCTSWFFLISDPQFAESHFDLNAAPTHRLLLPCLD 67
P LP +L+ EIL RLPV SLL+F+CVC SW LISDP+FA++H L+ +P P
Sbjct: 118 PPLPFDLVVEILCRLPVNSLLQFRCVCKSWNSLISDPKFAKNH--LHCSP------PDFT 273
Query: 68 KNKLESLDLESSSLF 82
++ L D+E +
Sbjct: 274 RHHLMGSDIEDEDSY 318
>AI967342
Length = 450
Score = 44.7 bits (104), Expect(2) = 7e-11
Identities = 30/75 (40%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
Frame = -3
Query: 170 QAFSVKTNSCD----FKYVNAQYRDLGYHYRHGVFLNNSLHWLVTTTDTSFSNDTSFLVV 225
+ FS KTNS D FK+ G G+ LN +LHWLV D V+
Sbjct: 394 ETFSFKTNSWDQIEGFKFPKFA---CGTRIEAGLLLNGALHWLVYDADN--------FVI 248
Query: 226 IAYDLLEKSLSEIPL 240
IA+DL+ +SL EIPL
Sbjct: 247 IAFDLIGRSLFEIPL 203
Score = 38.9 bits (89), Expect(2) = 7e-11
Identities = 19/34 (55%), Positives = 22/34 (63%), Gaps = 1/34 (2%)
Frame = -1
Query: 256 KFYHVRVLGGCLSLCYKGGR-RDRAEIWVMKEYK 288
K H+RV+G CL LC R AEIW+MKEYK
Sbjct: 174 KEVHLRVMGDCLCLCNPNSSDRAMAEIWMMKEYK 73
>AV423520
Length = 468
Score = 63.2 bits (152), Expect = 1e-10
Identities = 30/44 (68%), Positives = 35/44 (79%), Gaps = 1/44 (2%)
Frame = +3
Query: 8 PTLPHELIAEILLRLPVRSLLRFKCVCTSWFFLIS-DPQFAESH 50
PTLP EL+ EIL RLPV+SLL+F+CVC SW LIS DP+FA H
Sbjct: 159 PTLPFELVVEILCRLPVKSLLQFRCVCKSWNSLISGDPKFARKH 290
>AW720409
Length = 575
Score = 59.7 bits (143), Expect = 1e-09
Identities = 48/160 (30%), Positives = 73/160 (45%), Gaps = 18/160 (11%)
Frame = +3
Query: 10 LPHELIAEILLRLPVRSLLRFKCVCTSWFFLI-SDPQFAESHFDLNAAPTHRLLL----- 63
LP EL EIL LPV++L+RF CV SW LI D F + H D + ++++L
Sbjct: 93 LPFELWIEILSWLPVKTLMRFSCVSKSWKSLIYQDRDFKKLHLDRSPKNNNQVILTLQKP 272
Query: 64 --------PCLDKNKLESLDLESSSLFVTLNLPPPCKSRDHNSLYFLGSCRGFMLL--AY 113
P + L+ + SSS+ + + + N +GSC G + L
Sbjct: 273 LFSGRTFFPFPVRRLLQDQEPSSSSIIDDIPFEEEDEDEEDNYHDTIGSCNGLVCLYGIN 452
Query: 114 DYNRQVIVWNPSTGFYKQILSFSDFMLDSL--YGFGYDNS 151
D+ +WNP+T F D ++ S + FGYD+S
Sbjct: 453 DHGLWFRLWNPATRFRFHKSPPLDAVIGSTLHFAFGYDHS 572
>TC13380
Length = 593
Score = 58.9 bits (141), Expect = 2e-09
Identities = 54/160 (33%), Positives = 77/160 (47%), Gaps = 3/160 (1%)
Frame = +1
Query: 225 VIAYDLLEKSLSEIPL-SPE--LAKPVLTAEGAPKFYHVRVLGGCLSLCYKGGRRDRAEI 281
+IA+DL+EK L EIP P+ L +P FY + V G SL ++D EI
Sbjct: 52 IIAFDLMEKRLLEIPQPKPDDLLPQPDDLFPQGFLFYRLWVHGRFFSLT--AFQQDTIEI 225
Query: 282 WVMKEYKVQSSWTKAFVVTDCDIPCIHFYPIRFIERGGVLGSNGNGRLMTFNAEGKLLEH 341
WVMK+YKVQSSWTK VV + YP+ + G ++ +G L ++ EG
Sbjct: 226 WVMKKYKVQSSWTKTLVVLSSKL----LYPLCSTKGGDIVMCSGI-NLTKYSDEG----- 375
Query: 342 HKYGQEIKNVRKDLAMYRESEYKNIQIYFEMYRESLLSFP 381
G +++N + ES +Y ES+LS P
Sbjct: 376 -LQGDQLENF-DHFGLVNES--------VPLYTESMLSLP 465
>BP044058
Length = 579
Score = 58.5 bits (140), Expect = 3e-09
Identities = 41/98 (41%), Positives = 47/98 (47%)
Frame = -2
Query: 285 KEYKVQSSWTKAFVVTDCDIPCIHFYPIRFIERGGVLGSNGNGRLMTFNAEGKLLEHHKY 344
KEYKVQSSWTK T F+PI F + G + GS NGRL+ N +GKLL
Sbjct: 578 KEYKVQSSWTKIVFSTHDIHTXPRFFPICFTKCGDIFGSTMNGRLLKLNDKGKLL----- 414
Query: 345 GQEIKNVRKDLAMYRESEYKNIQIYFEMYRESLLSFPS 382
RES+Y Y MY SLLS PS
Sbjct: 413 ----------AVWPRESKYH--LFYSGMYTASLLSLPS 336
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.133 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,498,100
Number of Sequences: 28460
Number of extensions: 148953
Number of successful extensions: 1796
Number of sequences better than 10.0: 228
Number of HSP's better than 10.0 without gapping: 1020
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1442
length of query: 527
length of database: 4,897,600
effective HSP length: 94
effective length of query: 433
effective length of database: 2,222,360
effective search space: 962281880
effective search space used: 962281880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0476b.10