Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0476a.1
         (57 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC8033 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-trans...    26  1.4
TC8963 homologue to UP|TBB3_SOYBN (P28551) Tubulin beta chain (F...    26  1.8
BP052714                                                               25  2.4
TC14277 similar to UP|Q9SHY3 (Q9SHY3) F1E22.9 (AT1G65720/F1E22_1...    25  3.1
TC19620 similar to UP|Q9LSZ0 (Q9LSZ0) Gb|AAD38255.1, partial (5%)      24  5.3
BP048413                                                               24  6.9
BP063974                                                               24  6.9
BP061069                                                               23  9.0

>TC8033 similar to UP|NLTP_CICAR (O23758) Nonspecific lipid-transfer
           protein precursor (LTP), partial (85%)
          Length = 707

 Score = 26.2 bits (56), Expect = 1.4
 Identities = 9/29 (31%), Positives = 20/29 (68%)
 Frame = -1

Query: 7   YRSTHELDANDVVSISMWVESHLNCVFFY 35
           ++STH+L A+ ++S+    ++ L C++ Y
Sbjct: 104 HKSTHKLHASHLLSLKENTKNKLQCMYLY 18


>TC8963 homologue to UP|TBB3_SOYBN (P28551) Tubulin beta chain (Fragment),
           partial (75%)
          Length = 985

 Score = 25.8 bits (55), Expect = 1.8
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = +1

Query: 5   VIYRSTHELDANDVVSISMWVESHL 29
           +IY S   + ++ +VSIS+W+  HL
Sbjct: 814 LIYESLLSISSHSLVSISLWLGLHL 888


>BP052714 
          Length = 511

 Score = 25.4 bits (54), Expect = 2.4
 Identities = 10/35 (28%), Positives = 18/35 (50%)
 Frame = +3

Query: 16 NDVVSISMWVESHLNCVFFYEDFSNSDPFILGIQT 50
          N   + S+   +HLNC F +  F N D   + +++
Sbjct: 51 NKTKTTSLAFPNHLNCFFSHRKFKNIDMLTIQVKS 155


>TC14277 similar to UP|Q9SHY3 (Q9SHY3) F1E22.9 (AT1G65720/F1E22_13),
          partial (33%)
          Length = 941

 Score = 25.0 bits (53), Expect = 3.1
 Identities = 9/23 (39%), Positives = 14/23 (60%)
 Frame = +3

Query: 24 WVESHLNCVFFYEDFSNSDPFIL 46
          W ++  N  F Y   S+SDP++L
Sbjct: 9  WADAWFNFKFLYISLSDSDPWLL 77


>TC19620 similar to UP|Q9LSZ0 (Q9LSZ0) Gb|AAD38255.1, partial (5%)
          Length = 574

 Score = 24.3 bits (51), Expect = 5.3
 Identities = 10/33 (30%), Positives = 17/33 (51%)
 Frame = +3

Query: 7   YRSTHELDANDVVSISMWVESHLNCVFFYEDFS 39
           + S HEL++N          ++L C +F+  FS
Sbjct: 153 FESNHELNSNQATPSGTVWRTNLTCHYFHLHFS 251


>BP048413 
          Length = 542

 Score = 23.9 bits (50), Expect = 6.9
 Identities = 8/19 (42%), Positives = 14/19 (73%)
 Frame = +2

Query: 28 HLNCVFFYEDFSNSDPFIL 46
          +L C+ F+E+ +N  PF+L
Sbjct: 2  NLICIIFFEEGANDFPFLL 58


>BP063974 
          Length = 408

 Score = 23.9 bits (50), Expect = 6.9
 Identities = 10/36 (27%), Positives = 18/36 (49%)
 Frame = -3

Query: 5   VIYRSTHELDANDVVSISMWVESHLNCVFFYEDFSN 40
           +++ S ++   N   S   W E+H+ C F    FS+
Sbjct: 301 IMFLSFYK*QCNTCFSFDSWKENHI*CSFLIIFFSS 194


>BP061069 
          Length = 284

 Score = 23.5 bits (49), Expect = 9.0
 Identities = 12/33 (36%), Positives = 15/33 (45%)
 Frame = -3

Query: 14  DANDVVSISMWVESHLNCVFFYEDFSNSDPFIL 46
           D N  V   MW+  +     FY  F+NS  F L
Sbjct: 126 DGN*GVRKGMWISLYFTVFHFYGFFTNSGLFHL 28


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.335    0.144    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,141,811
Number of Sequences: 28460
Number of extensions: 12302
Number of successful extensions: 128
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of query: 57
length of database: 4,897,600
effective HSP length: 33
effective length of query: 24
effective length of database: 3,958,420
effective search space: 95002080
effective search space used: 95002080
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 49 (23.5 bits)


Lotus: description of TM0476a.1