BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0400a.8
(721 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP039459 281 2e-76
BP066990 254 4e-68
AW164028 131 4e-31
TC17621 similar to UP|BAD07830 (BAD07830) Kinesin light chain-li... 124 5e-29
BP038767 74 6e-14
TC18281 34 0.072
BP040546 31 0.79
NP459594 Krm protein [Lotus japonicus] 29 2.3
TC18999 similar to UP|APKB_ARATH (P46573) Protein kinase APK1B ... 29 3.0
TC12428 similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-lin... 28 5.1
BP031459 28 6.7
AW719699 28 6.7
AV771187 28 6.7
BP058585 27 8.8
>BP039459
Length = 535
Score = 281 bits (720), Expect = 2e-76
Identities = 141/143 (98%), Positives = 141/143 (98%)
Frame = +1
Query: 1 MPERAMDELNMNIAGEGPSGSYTPHKDNFNQQASPRSTLSPRSIQSSDSIDLAIDGVVDT 60
MPERAMDELNMNIAGEGPSGSYTPHKDNFNQQASPRSTLSPRSIQSSDSIDLAIDGVVDT
Sbjct: 106 MPERAMDELNMNIAGEGPSGSYTPHKDNFNQQASPRSTLSPRSIQSSDSIDLAIDGVVDT 285
Query: 61 SIEQLYYNVCEMRSSDQSPSRASFYSYGEESRIDSELGHLVGGILEITKEVVTENKEESN 120
SIEQLYYNVCEMRSSDQSPSRASFYSYGEESRIDSELGHLVGGILEITKEVVTENKEESN
Sbjct: 286 SIEQLYYNVCEMRSSDQSPSRASFYSYGEESRIDSELGHLVGGILEITKEVVTENKEESN 465
Query: 121 GNAAEKDIVSCGKEATKKDNNQS 143
GNAAEKD VSCGKEATK DNNQS
Sbjct: 466 GNAAEKDXVSCGKEATKXDNNQS 534
>BP066990
Length = 528
Score = 254 bits (648), Expect = 4e-68
Identities = 123/176 (69%), Positives = 151/176 (84%)
Frame = +1
Query: 419 GQEQDVASVDSSIGDAYLALARYDEAVFSYQKALTVFKSTKGENHPNVASVYVRLADLYN 478
GQE +VASVD SIGD YL+++RYDEAVF+Y+KALTVFK+ KGENHP V SV+VRLADL+N
Sbjct: 1 GQEVEVASVDCSIGDTYLSMSRYDEAVFAYEKALTVFKTGKGENHPAVGSVFVRLADLHN 180
Query: 479 KIGKFKDSKSYCENALRIFGKSKPGIPPEEIANGLIDVAAIYQSMNDLEKGLKLLKKALK 538
+ GK K+SKSYC++ALRI+ PG+ PEEIA+GL +V+AIY+SMN+LEK LKLL+KAL
Sbjct: 181 RTGKIKESKSYCDSALRIYENPMPGVNPEEIASGLTNVSAIYESMNELEKALKLLQKALL 360
Query: 539 IYGHAPGQQSTIAGIEAQMGVMYYMLGNYSDSYNIFKSAVAKFRASGEKKSALFGI 594
IY +PGQQS+IAGIEAQMGVMYYMLGNY++SYN K+A+ K RA GEKKS+ GI
Sbjct: 361 IYNDSPGQQSSIAGIEAQMGVMYYMLGNYTESYNTLKNAITKLRAIGEKKSSFIGI 528
>AW164028
Length = 313
Score = 131 bits (329), Expect = 4e-31
Identities = 60/99 (60%), Positives = 83/99 (83%)
Frame = +2
Query: 456 KSTKGENHPNVASVYVRLADLYNKIGKFKDSKSYCENALRIFGKSKPGIPPEEIANGLID 515
K++KGENHP+VASV+VRLADLY++ GK ++SKSYCENA+RI+ K PG PEEIA GL +
Sbjct: 17 KASKGENHPSVASVFVRLADLYHRTGKLRESKSYCENAMRIYSKPVPGTTPEEIAGGLTE 196
Query: 516 VAAIYQSMNDLEKGLKLLKKALKIYGHAPGQQSTIAGIE 554
V+AI++S+++ E+ LKLL++A+ + PGQQST+AGIE
Sbjct: 197 VSAIFESVDEPEEALKLLQRAMNLLEEKPGQQSTVAGIE 313
>TC17621 similar to UP|BAD07830 (BAD07830) Kinesin light chain-like protein,
partial (17%)
Length = 547
Score = 124 bits (311), Expect = 5e-29
Identities = 65/92 (70%), Positives = 74/92 (79%)
Frame = +3
Query: 628 GQYHPDTLGVYSNLAGTYDAMGRVDDAIEILEFVVGMREEKLGTANPDVDDEKRRLAELL 687
G H DTLGVYSNLA TYDAMGRV DAIEILE+V+ +REEKLG ANPD +DEKRRLAELL
Sbjct: 6 GPCHQDTLGVYSNLAATYDAMGRVGDAIEILEYVLKLREEKLGIANPDFEDEKRRLAELL 185
Query: 688 KEAGRGRNRKSKRSLETLLDANSRLIKNNSIK 719
KEAG+ R+RK+K SLE L+D NS+ K K
Sbjct: 186 KEAGKTRDRKAK-SLENLIDPNSKRTKKEGNK 278
>BP038767
Length = 561
Score = 74.3 bits (181), Expect = 6e-14
Identities = 48/113 (42%), Positives = 62/113 (54%), Gaps = 2/113 (1%)
Frame = +2
Query: 142 QSHSPSSRIIEGSAKS-TPKSRNLRERPSTDKRSERSSRKGSLYPMRKHRSLALRGIIEE 200
Q+ +P+ R + + TP L ++P + S RSS K + +L
Sbjct: 257 QTQTPTRRSEPAATNNKTPSPSPLPKKPPSPSPSTRSSAKKKTPETPTNPDASL------ 418
Query: 201 RIATGLDNPDLGPFLLKQTRDMISSGENPQKALDLALRALKSFEMC-ADGKPS 252
DNPDLGPFLLK RD I+SGE P KALD A+RA KSFE+C ADG+PS
Sbjct: 419 ------DNPDLGPFLLKLARDTIASGEGPTKALDYAIRASKSFEICAADGEPS 559
>TC18281
Length = 685
Score = 34.3 bits (77), Expect = 0.072
Identities = 22/75 (29%), Positives = 39/75 (51%), Gaps = 3/75 (4%)
Frame = +3
Query: 106 EITKEVVTENKEESNGNAAEKDIVSCGKE---ATKKDNNQSHSPSSRIIEGSAKSTPKSR 162
E KE+V E K++S+ N A+ GKE ++ + S S S+R ++ S K
Sbjct: 120 EALKEIVVETKKQSSSNVAKNQTADDGKELPNSSAPRDTSSASTSNRAVDEDRFS--KHN 293
Query: 163 NLRERPSTDKRSERS 177
+L+ +P+ + S+ S
Sbjct: 294 SLKSKPNLGEESKGS 338
>BP040546
Length = 577
Score = 30.8 bits (68), Expect = 0.79
Identities = 22/80 (27%), Positives = 44/80 (54%), Gaps = 1/80 (1%)
Frame = +3
Query: 494 LRIFGKSKPGIPPEEIANG-LIDVAAIYQSMNDLEKGLKLLKKALKIYGHAPGQQSTIAG 552
LR+ +++ +PP + NG L+ A S+ + +GLK+ K L +Y H P + ++
Sbjct: 3 LRLKVENQNLVPPPSLPNGNLVAAAPRRYSVAAIMEGLKVSKSKLTVYLH-PSKSKQVS- 176
Query: 553 IEAQMGVMYYMLGNYSDSYN 572
E+ + + ML +SD+++
Sbjct: 177 -ESVLRELSSMLFTFSDTFD 233
>NP459594 Krm protein [Lotus japonicus]
Length = 632
Score = 29.3 bits (64), Expect = 2.3
Identities = 14/62 (22%), Positives = 30/62 (47%)
Frame = +3
Query: 309 YAMMGNIENSILFYTAGLEIQGQVLGETDPRFGETCRYVAEAHVQALQFDEAERLCQMAL 368
+ + G ++ + + + ++ + G+ DP C +AE + +FD+AE L A+
Sbjct: 411 FFLQGKLDEAEKLFLSAVQEAKEGFGKQDPHVASACNNLAEFYRIRKEFDKAEPLYLEAI 590
Query: 369 EI 370
I
Sbjct: 591 NI 596
>TC18999 similar to UP|APKB_ARATH (P46573) Protein kinase APK1B , partial
(6%)
Length = 425
Score = 28.9 bits (63), Expect = 3.0
Identities = 29/92 (31%), Positives = 44/92 (47%), Gaps = 21/92 (22%)
Frame = +3
Query: 113 TENKEES--NGNAAEKDIVSCGKEATKKD-NNQSH----------SPSSRIIEGSAKSTP 159
T K ES N + K + + K+ + KD +QS S SS+++EGSA TP
Sbjct: 75 THIKAESLLNTGLSSKHVSADAKDPSNKDPGDQSRTSSEVADDLSSQSSKVLEGSAPQTP 254
Query: 160 K-------SRNLRERPSTD-KRSERSSRKGSL 183
+ S NL+ P T+ K + R+ R S+
Sbjct: 255 RSVCEIVQSSNLKSFPLTELKVATRNFRADSM 350
>TC12428 similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-linked
oxidoreductase family), partial (26%)
Length = 648
Score = 28.1 bits (61), Expect = 5.1
Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
Frame = +1
Query: 167 RPSTDKRSERSSRKGSLYPMRKHRSLALRGIIEERIATGLDNPDLGPFLLKQTRDM--IS 224
R T KR RSSR+ +R+H+ +G+ + P L P LL+ R S
Sbjct: 88 RSPTPKRRRRSSRRRRFLQLRRHQP-RRQGLRRHEVR---PPPSLNPPLLRHRRGPRGES 255
Query: 225 SGENPQ 230
SGE+P+
Sbjct: 256 SGEDPK 273
>BP031459
Length = 388
Score = 27.7 bits (60), Expect = 6.7
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = -3
Query: 81 RASFYSYGEESRIDSELGHLVGGILEIT 108
R S + YG++SR+D+ + L G I+EI+
Sbjct: 347 RFSTFDYGQDSRVDTIIWILSGEIMEIS 264
>AW719699
Length = 489
Score = 27.7 bits (60), Expect = 6.7
Identities = 13/48 (27%), Positives = 25/48 (52%)
Frame = +2
Query: 133 KEATKKDNNQSHSPSSRIIEGSAKSTPKSRNLRERPSTDKRSERSSRK 180
+ + + Q ++ S I+ G A+ TP+ R +PS ++RS R+
Sbjct: 239 RRRSAEGGKQRYTRRSSIVNGGARGTPRKSW*RFKPSPNRRSPEPVRQ 382
>AV771187
Length = 484
Score = 27.7 bits (60), Expect = 6.7
Identities = 19/58 (32%), Positives = 30/58 (50%)
Frame = -3
Query: 601 LACVQRYAINEAADLFEEARTILEKEYGQYHPDTLGVYSNLAGTYDAMGRVDDAIEIL 658
+A +RY I A +LFE+ K+ G P+ + Y +L Y A +D A+E+L
Sbjct: 260 MAAARRYRIQRALELFEDM-----KKKG-CAPNRV-TYDSLIRYYSATNEIDRAVEVL 108
>BP058585
Length = 395
Score = 27.3 bits (59), Expect = 8.8
Identities = 24/101 (23%), Positives = 39/101 (37%), Gaps = 9/101 (8%)
Frame = +2
Query: 155 AKSTPKSRNLRERPSTDKRSERSSRKGSLYPMRKHRSLALRG---------IIEERIATG 205
+K PKS + + + + R +L + K S+ L I + + G
Sbjct: 11 SKPHPKSSSSHLHKAHNLHCQEEHRVNALQFLHKQSSIWLESRENNRPVLSIFKHNLQDG 190
Query: 206 LDNPDLGPFLLKQTRDMISSGENPQKALDLALRALKSFEMC 246
L N + FL+ + PQ+ L + R LKS E C
Sbjct: 191 LKNMNKIIFLIHNMIIKLQLNIKPQRRLHILKRFLKSAERC 313
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.132 0.364
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,281,689
Number of Sequences: 28460
Number of extensions: 103737
Number of successful extensions: 425
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 423
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 425
length of query: 721
length of database: 4,897,600
effective HSP length: 97
effective length of query: 624
effective length of database: 2,136,980
effective search space: 1333475520
effective search space used: 1333475520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0400a.8