BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0399.4
(1400 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780009 135 6e-32
BP066094 115 2e-28
AV427921 111 7e-25
BP084332 105 6e-23
BP075943 102 3e-22
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 90 2e-18
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 80 2e-15
BP063694 79 5e-15
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 70 6e-14
BP043850 71 1e-12
AV424544 70 3e-12
BP057233 68 9e-12
AU088980 62 8e-10
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 60 2e-09
BP033602 59 4e-09
AV766088 57 2e-08
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 53 4e-07
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 53 4e-07
AV766665 53 4e-07
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 52 8e-07
>AV780009
Length = 529
Score = 135 bits (339), Expect = 6e-32
Identities = 74/170 (43%), Positives = 95/170 (55%)
Frame = -1
Query: 1206 AAKRILRYLKGTPNHGVFFPKHLPSQKEDGNLHLVAYTDSDWCGDQVDRRSTMGYVFFFG 1265
AA R+LRY+KG P G+FF D L L AY+DSDW G RRS GY F G
Sbjct: 526 AATRVLRYVKGAPAQGLFF-------SADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLG 368
Query: 1266 KAPISWSSKKQAAVALSTCEAEYIAACSAACQGLWIQALLEELGLKTDEAVQLMVDNKSA 1325
+ ISW +KKQ V+ S+ EAEY A + C+ W+ L + L L V L DN+SA
Sbjct: 367 TSLISWRTKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSA 188
Query: 1326 IDLAKNPVSHGRSKHIETKYHFLRDQVSKEKIKLQHCGTDLQIADVFTKP 1375
+ +A NP H R+KHIE H +R ++ I L T Q+AD+FTKP
Sbjct: 187 LHIAHNPTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>BP066094
Length = 532
Score = 115 bits (289), Expect(2) = 2e-28
Identities = 58/150 (38%), Positives = 97/150 (64%)
Frame = +2
Query: 964 ARLETVRMIVALASWKNWDLWQLDVKSAFLNGPLEEEVYITQPPGFEIKGSEHKVLKLRK 1023
++ E +R++++ + N L Q++VKSAFLNG + EEVY+ QPPG E + + + KL+K
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1024 ALYGLKQAPRAWNKRIDTFLSQTGFHKCSVEHGVYVKSCDSGGVLLLCLYVDDLLITGSS 1083
+LYGLKQAPRAW +R+ +FL + + V+ ++ K+ +L++ +YVDD++ ++
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTY-KDDILIVQIYVDDIIFGSAN 442
Query: 1084 YSKIQAVKRSLNNEFEMTDLGKLSYFLGIE 1113
S + + EFEM +G+L YFLGI+
Sbjct: 443 PSLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 28.1 bits (61), Expect(2) = 2e-28
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +3
Query: 948 FMQRGGLDYSEVFAPVAR 965
+ Q+ G+DY+E FAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
>AV427921
Length = 284
Score = 111 bits (278), Expect = 7e-25
Identities = 51/91 (56%), Positives = 66/91 (72%)
Frame = +1
Query: 655 TAPYTPQHNGVAERRNRTVLNMVRSMLKGKSLPHRFCGEAVMTAVYVLNLCPTKSVDSQV 714
TA YTPQ NGV+ER+NRT+LNMVRS+L LP F EAVM ++++LN PT V + +
Sbjct: 10 TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMM 189
Query: 715 PEAVWSGRKPSVKHLRIFGCLCHKHIPDQRR 745
PE WSGR+P+V H RI CL + H+PDQ+R
Sbjct: 190 PEEAWSGRQPAVDHFRISRCLAYAHVPDQKR 282
>BP084332
Length = 368
Score = 105 bits (261), Expect = 6e-23
Identities = 53/111 (47%), Positives = 73/111 (65%)
Frame = +2
Query: 1276 QAAVALSTCEAEYIAACSAACQGLWIQALLEELGLKTDEAVQLMVDNKSAIDLAKNPVSH 1335
Q+ +ALST EAEYI+A + Q LW++ LE+ + + + + DN +AI L+KNP+ H
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILH 208
Query: 1336 GRSKHIETKYHFLRDQVSKEKIKLQHCGTDLQIADVFTKPLKADRFKTLKK 1386
R+KHIE KYHF+RD V K + L+ TD Q AD+FTKPL DRF + K
Sbjct: 209 SRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFILK 361
>BP075943
Length = 547
Score = 102 bits (255), Expect = 3e-22
Identities = 57/163 (34%), Positives = 92/163 (55%), Gaps = 1/163 (0%)
Frame = -3
Query: 1064 SGGVLLLCLYVDDLLITGSSYSKIQAVKRSLNNEFEMTDLGKLSYFLGIEFVQTGEGILM 1123
SG L LYVDD+L+ G+ +IQ VK SL+++F + DLG+ YFLG+E ++ GI++
Sbjct: 539 SGSFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVL 360
Query: 1124 HQRKYILEVLKRFNLLSCNPAETPVEGNLK-LGLCEEEAEVDSTMFRQLVGCLRFICHSR 1182
+QRKY L+++ P N + LG D +R++VG L ++ +R
Sbjct: 359 NQRKYALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTR 180
Query: 1183 LEISYGVGLVSRFMSCPRQSHLAAAKRILRYLKGTPNHGVFFP 1225
+I++ V +S+F+S P H L+YL G+P G+F+P
Sbjct: 179 PDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYP 51
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 90.1 bits (222), Expect = 2e-18
Identities = 48/146 (32%), Positives = 78/146 (52%), Gaps = 8/146 (5%)
Frame = +2
Query: 1040 DTFLSQTGFHKCSVEHGVYVKSCDSGGVLLLCLYVDDLLITGSSYSKIQAVKRSLNNEFE 1099
D+F+ G+++ S +H Y K D ++L LYVDD+L+ G + ++Q +K L EF+
Sbjct: 5 DSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFD 184
Query: 1100 MTDLGKLSYFLGIEFVQTGEG--ILMHQRKYILEVLKRFNLLSCNPAETPVEGNLKLGLC 1157
M DLG + LG++ + + I + Q+ Y+ +VL+RFN+ CNP TP+ N KL
Sbjct: 185 MKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSS 364
Query: 1158 ------EEEAEVDSTMFRQLVGCLRF 1177
E E+ + VG L +
Sbjct: 365 MIPSSEAERMEMSRVPYASAVGSLMY 442
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 80.1 bits (196), Expect = 2e-15
Identities = 35/60 (58%), Positives = 48/60 (79%)
Frame = +3
Query: 1332 PVSHGRSKHIETKYHFLRDQVSKEKIKLQHCGTDLQIADVFTKPLKADRFKTLKKMMNVL 1391
P+ + +IETKYHFLRDQV+K KI L+HCGTDLQ+AD+ TK LK +RF+ ++ M+NV+
Sbjct: 45 PMDVVNTLNIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNVV 224
Score = 50.1 bits (118), Expect = 2e-06
Identities = 23/23 (100%), Positives = 23/23 (100%)
Frame = +2
Query: 1320 VDNKSAIDLAKNPVSHGRSKHIE 1342
VDNKSAIDLAKNPVSHGRSKHIE
Sbjct: 2 VDNKSAIDLAKNPVSHGRSKHIE 70
>BP063694
Length = 511
Score = 79.0 bits (193), Expect = 5e-15
Identities = 48/118 (40%), Positives = 64/118 (53%), Gaps = 6/118 (5%)
Frame = -2
Query: 502 LWHQRFGHLNFKDLSSLKSKDMVHGLSQIK--LPSKVC-ENCLVSKQPRAPFSSFTPTRS 558
LWH R GH+NF + L HG + LP +C +C ++K R F R+
Sbjct: 360 LWHSRLGHVNFDIIKQLHK----HGCLDVSSILPKPICCTSCQMAKSKRLVFHD-NNKRA 196
Query: 559 TAVLDVIYSDVCGPFETASIGGNKYFASFIDEYSRKMWVYLLKTKSEVFSV---FKVF 613
+AVLD+I+ D+ GP ASI G YF F+D++SR W Y LK KS+ V FKVF
Sbjct: 195 SAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVF 22
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 69.7 bits (169), Expect(2) = 6e-14
Identities = 49/150 (32%), Positives = 74/150 (48%), Gaps = 1/150 (0%)
Frame = -1
Query: 1125 QRKYILEVLKRFNLLSCNPAETPVEGN-LKLGLCEEEAEVDSTMFRQLVGCLRFICHSRL 1183
Q+ Y+ ++LK+F + + T + G ++ G VDST ++ L+G +R++ R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1184 EISYGVGLVSRFMSCPRQSHLAAAKRILRYLKGTPNHGVFFPKHLPSQKEDGNLHLVAYT 1243
+I GVGL SRFM P H A+R LRY+KGT G+F ++ T
Sbjct: 559 DIVCGVGLRSRFME-P*DCH*QGAQRSLRYIKGTLKDGIF---------------MLVIT 428
Query: 1244 DSDWCGDQVDRRSTMGYVFFFGKAPISWSS 1273
+ Q+ R GY F G+ ISWSS
Sbjct: 427 M*NSLDTQIVTRP--GYAFHLGRGAISWSS 344
Score = 25.8 bits (55), Expect(2) = 6e-14
Identities = 16/36 (44%), Positives = 21/36 (57%)
Frame = -2
Query: 1276 QAAVALSTCEAEYIAACSAACQGLWIQALLEELGLK 1311
Q+ VAL EA YIAA S A Q + + +LE + K
Sbjct: 342 QSVVALYANEA*YIAAESCATQAI*LTRILEVMHQK 235
>BP043850
Length = 515
Score = 70.9 bits (172), Expect = 1e-12
Identities = 38/93 (40%), Positives = 51/93 (53%), Gaps = 1/93 (1%)
Frame = -1
Query: 1299 LWIQALLEELGLKTDEAVQLMVDNKSAIDLAKNPVSHGRSKHIETKYHFLRDQVSKEKIK 1358
+W++ LL ELG + L DN SAI +A NPV H ++HIE H +R+ + I
Sbjct: 506 IWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRRVIT 327
Query: 1359 LQHCGTDLQIADVFTKPLKADRFKTL-KKMMNV 1390
L H T +QIAD+ TK L R L K+M V
Sbjct: 326 LPHVSTSVQIADILTKSLTRQRHNFLVSKLMLV 228
>AV424544
Length = 276
Score = 69.7 bits (169), Expect = 3e-12
Identities = 32/91 (35%), Positives = 59/91 (64%)
Frame = +3
Query: 1038 RIDTFLSQTGFHKCSVEHGVYVKSCDSGGVLLLCLYVDDLLITGSSYSKIQAVKRSLNNE 1097
++ ++L G+ + + +H ++ K D+ ++L +YVDDL++ G+ ++IQ VK L+ +
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVIL-VYVDDLILAGNDLNEIQCVKNKLDIQ 182
Query: 1098 FEMTDLGKLSYFLGIEFVQTGEGILMHQRKY 1128
F + DLG L YFLG+E ++ G+ + QRKY
Sbjct: 183 FRIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>BP057233
Length = 473
Score = 68.2 bits (165), Expect = 9e-12
Identities = 35/74 (47%), Positives = 45/74 (60%)
Frame = -2
Query: 1318 LMVDNKSAIDLAKNPVSHGRSKHIETKYHFLRDQVSKEKIKLQHCGTDLQIADVFTKPLK 1377
L DN SA LA NPV H RSKHIE H++RDQV + ++ + + T QIAD TKPL
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1378 ADRFKTLKKMMNVL 1391
RF L+ + V+
Sbjct: 265 HTRFSQLRDKLGVI 224
>AU088980
Length = 360
Score = 61.6 bits (148), Expect = 8e-10
Identities = 28/100 (28%), Positives = 53/100 (53%)
Frame = +2
Query: 663 NGVAERRNRTVLNMVRSMLKGKSLPHRFCGEAVMTAVYVLNLCPTKSVDSQVPEAVWSGR 722
N + ER++R +LN+ R+++ LP AV A Y++N P+ + Q P +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 723 KPSVKHLRIFGCLCHKHIPDQRRRKLDDKSETMIFIGYSS 762
P++ L++FG LC+ +KLD ++ +F+G+ +
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKT 301
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 60.1 bits (144), Expect = 2e-09
Identities = 29/82 (35%), Positives = 46/82 (55%)
Frame = +1
Query: 1300 WIQALLEELGLKTDEAVQLMVDNKSAIDLAKNPVSHGRSKHIETKYHFLRDQVSKEKIKL 1359
W+ LL++L + ++ + DN SA +A NPV H R+KHIE H +R+++ K I L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1360 QHCGTDLQIADVFTKPLKADRF 1381
+ +AD++TK L F
Sbjct: 184 LPISSSEPLADIYTKALSPQNF 249
>BP033602
Length = 533
Score = 59.3 bits (142), Expect = 4e-09
Identities = 33/97 (34%), Positives = 54/97 (55%)
Frame = +3
Query: 496 LEDNSWLWHQRFGHLNFKDLSSLKSKDMVHGLSQIKLPSKVCENCLVSKQPRAPFSSFTP 555
+ D LWH R GH NF+ L L D+ + S CE+C+++K RAP+ S P
Sbjct: 258 VRDQIMLWHNRLGHPNFQYLRHL-FPDL---FKNVNCSSLECESCVLAKNQRAPYYS-QP 422
Query: 556 TRSTAVLDVIYSDVCGPFETASIGGNKYFASFIDEYS 592
++ +I+SDV GP + + G ++F +FID+++
Sbjct: 423 YHASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV766088
Length = 501
Score = 57.4 bits (137), Expect = 2e-08
Identities = 32/106 (30%), Positives = 55/106 (51%)
Frame = -3
Query: 502 LWHQRFGHLNFKDLSSLKSKDMVHGLSQIKLPSKVCENCLVSKQPRAPFSSFTPTRSTAV 561
LWH R GH + L L+S + ++ S VCE+C+ K R PF S +
Sbjct: 307 LWHSRLGHPSSSALRYLRSNKFIS--YELLNYSPVCESCVFGKHVRLPFVSSNNV-TVMP 137
Query: 562 LDVIYSDVCGPFETASIGGNKYFASFIDEYSRKMWVYLLKTKSEVF 607
D+++SD+ S G++++ F+D+++ +W + L KS+VF
Sbjct: 136 FDILHSDLWTS-PVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 52.8 bits (125), Expect = 4e-07
Identities = 30/84 (35%), Positives = 47/84 (55%)
Frame = +3
Query: 1293 SAACQGLWIQALLEELGLKTDEAVQLMVDNKSAIDLAKNPVSHGRSKHIETKYHFLRDQV 1352
S C+ LW+ L +L + + V + DN+SA+ LA N V H R+++IE H + +V
Sbjct: 9 SHPCEALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKV 188
Query: 1353 SKEKIKLQHCGTDLQIADVFTKPL 1376
+ L H + Q+ADVFTK +
Sbjct: 189 LFGILHLLHVPSSDQVADVFTKTI 260
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 52.8 bits (125), Expect = 4e-07
Identities = 29/96 (30%), Positives = 51/96 (52%)
Frame = +1
Query: 1296 CQGLWIQALLEELGLKTDEAVQLMVDNKSAIDLAKNPVSHGRSKHIETKYHFLRDQVSKE 1355
C+ W+ +L++L + L D++SA +A N V H R+KH++ H +R+++ +
Sbjct: 1 CEL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAK 180
Query: 1356 KIKLQHCGTDLQIADVFTKPLKADRFKTLKKMMNVL 1391
L + Q AD+ TKPL++ F L + VL
Sbjct: 181 LFHLLPISSVDQTADILTKPLESGPFSHLVSKLGVL 288
>AV766665
Length = 601
Score = 52.8 bits (125), Expect = 4e-07
Identities = 36/99 (36%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
Frame = +3
Query: 1190 GLVSRFMSCPRQSHLAA-AKRILRYLKGTPNHGVFFPKHLPSQKEDGNLHLVAYTDSDWC 1248
GLV + R S L RI YLK G F K +G + +T +D+
Sbjct: 312 GLVQKTYGKNRWSSLKTFTTRIF*YLKANSRRGPLFQK-------EGKSSMDGFTYADYL 470
Query: 1249 GDQVDRRSTMGYVFFFGKAPISWSSKKQAAVALSTCEAE 1287
G VDR STMGY F ++W SK+Q +A S+ EAE
Sbjct: 471 GSIVDRLSTMGYYMFLSGNLVTWRSKQQNIIARSSGEAE 587
Score = 30.4 bits (67), Expect = 2.0
Identities = 18/46 (39%), Positives = 24/46 (52%)
Frame = +1
Query: 1070 LCLYVDDLLITGSSYSKIQAVKRSLNNEFEMTDLGKLSYFLGIEFV 1115
LC+YVD + I G + Q ++ L +F DL KL Y IE V
Sbjct: 115 LCVYVDHMTIVGDDEVEKQILREKLAPQF---DLKKLKYISVIEVV 243
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 51.6 bits (122), Expect = 8e-07
Identities = 34/119 (28%), Positives = 59/119 (49%)
Frame = +3
Query: 1264 FGKAPISWSSKKQAAVALSTCEAEYIAACSAACQGLWIQALLEELGLKTDEAVQLMVDNK 1323
F ++W S+ Q VALST EAE+IAA A + LW++ L+ + + +V K
Sbjct: 12 FAGGAVAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSHPILCCIVITK 191
Query: 1324 SAIDLAKNPVSHGRSKHIETKYHFLRDQVSKEKIKLQHCGTDLQIADVFTKPLKADRFK 1382
+ LA+ + + LRD ++ + ++L+ TD AD+ TK L ++ +
Sbjct: 192 ALFTLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLE 368
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,451,338
Number of Sequences: 28460
Number of extensions: 322761
Number of successful extensions: 1603
Number of sequences better than 10.0: 81
Number of HSP's better than 10.0 without gapping: 1559
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1588
length of query: 1400
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1298
effective length of database: 1,994,680
effective search space: 2589094640
effective search space used: 2589094640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0399.4