BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0391.10
(1159 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP030148 62 4e-10
BF177509 54 1e-07
AV406521 36 4e-05
TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich... 43 3e-04
TC8967 similar to UP|FXGA_HUMAN (P55316) Forkhead box protein G1... 42 4e-04
TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II ... 42 7e-04
TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-li... 40 0.002
AW720051 40 0.002
TC14287 similar to UP|Q9LEU7 (Q9LEU7) Serine/threonine protein k... 39 0.005
TC7897 weakly similar to UP|ENL2_ARATH (Q9T076) Early nodulin-li... 39 0.006
CB829112 38 0.008
BP049447 38 0.008
BP049972 38 0.008
TC14720 UP|Q9FSH3 (Q9FSH3) Ammonium transporter (AMT1.1), complete 38 0.008
CN825698 38 0.008
TC17263 homologue to UP|RUBB_PEA (P08927) RuBisCO subunit bindin... 38 0.008
TC13588 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall... 38 0.011
AV780471 37 0.014
TC14068 homologue to UP|Q9SLR7 (Q9SLR7) Thiamin biosynthetic enz... 33 0.016
TC13980 similar to UP|Q9SGT0 (Q9SGT0) T6H22.18 protein (F14J16.3... 37 0.018
>BP030148
Length = 398
Score = 62.4 bits (150), Expect = 4e-10
Identities = 31/117 (26%), Positives = 60/117 (50%)
Frame = +3
Query: 661 QGLIPAMQEVMPGVHHRFCAMHLWRNFTKQWKDKEMKNVVWECARARTIPEFNDKMDKLK 720
+G++ ++ P H FC HL +F K++ + + N++WE A T+ EF K+ +++
Sbjct: 45 KGIVDGVEANFPTAFHGFCMRHLSDSFRKEFNNTMLVNLLWEAAHVLTVIEFESKILEIE 224
Query: 721 AKNVQAWNYLNKWPKEAWTKAYFRDHPKIDNICNNACEAFNAKILKYRGKPILTLLE 777
+ A ++ + P W AYF + + ++ N E+ + IL G PI+ + E
Sbjct: 225 EISQDAAYWIRRIPPRLWATAYF-EGQRFGHLTANIVESLDTWILDASGLPIVQMTE 392
>BF177509
Length = 472
Score = 54.3 bits (129), Expect = 1e-07
Identities = 35/140 (25%), Positives = 62/140 (44%)
Frame = +1
Query: 729 YLNKWPKEAWTKAYFRDHPKIDNICNNACEAFNAKILKYRGKPILTLLEEVRCYIMRTVA 788
++ + P W AYF + ++ N E+ N IL+ G PI+ ++E +R +M
Sbjct: 19 WIRRIPPRLWATAYFEGQ-RFGHLTANIVESLNTWILEASGLPIIQMMECIRRQLMTWFN 195
Query: 789 NNKMKLRGHQGMLPPLQQSRLEHQKEASNGWTPVWCGDGDGYEYQLQCWGETVGVHLSNG 848
+ +L P + + E + + + + E+++ + V + N
Sbjct: 196 ERRETSMQWASILVPSAERCVAEALERARTYQVLRANEA---EFEVISHEGSNIVDIRNR 366
Query: 849 TCHCRFWQLTGMPCKHVVAA 868
C CR WQL G+PC H VAA
Sbjct: 367 CCLCRGWQLYGLPCAHAVAA 426
>AV406521
Length = 267
Score = 35.8 bits (81), Expect(2) = 4e-05
Identities = 20/45 (44%), Positives = 25/45 (55%), Gaps = 4/45 (8%)
Frame = +1
Query: 577 IAYAVVNVENKDNWKWFLELLH----ADLGDYQQYGWCFISDMQK 617
+A+ VV+VEN DNW WF LH A Q G F+SD Q+
Sbjct: 1 LAFGVVDVENDDNWTWFRSELHKALEASTESMPQMG--FLSDGQR 129
Score = 29.3 bits (64), Expect(2) = 4e-05
Identities = 12/52 (23%), Positives = 26/52 (49%)
Frame = +3
Query: 655 MFYVFDQGLIPAMQEVMPGVHHRFCAMHLWRNFTKQWKDKEMKNVVWECARA 706
+F + +G ++ H FC HL + K++K+ + +++W+ A A
Sbjct: 108 IFVRWAKGYYRRSEDEFSNSSHTFCMRHLSESIGKEFKNSRLVHLLWKAAYA 263
>TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich protein 1
(NDPP-1). {Mus musculus;}, partial (8%)
Length = 1134
Score = 42.7 bits (99), Expect = 3e-04
Identities = 39/118 (33%), Positives = 52/118 (44%)
Frame = -3
Query: 113 EDGAVVVIESIVADEDGLGCGVGNVSEEGSGIGAEDGNERVNGEGSEIGADNGNERVTGE 172
E+GA +V+E E+GL G E G+ GAE+G +G + G +G G
Sbjct: 442 ENGAAIVVEC--GPEEGL-VGEAKDEEVGTRWGAEEGG----CDGVDFGRGHG---FVGC 293
Query: 173 GSGIGSVHGNERVTGEGSGIGACNGNESVTGEGSGIGGVHGNERVAVEDVNEIVNEEE 230
G G+ + EGSG G G G GSG G GNE ++ E EEE
Sbjct: 292 GGGVEG-----GIVEEGSGDGRWEGRGGACGGGSGGGDGGGNEGEG*DEEGEEEEEEE 134
Score = 29.3 bits (64), Expect = 3.8
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3
Query: 95 PLSMIPPPPPPPPPPP 110
P +PPP PPP PPP
Sbjct: 171 PSPSLPPPSPPPLPPP 218
Score = 28.5 bits (62), Expect = 6.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +3
Query: 91 PHPKPLSMIPPPPPPPPP 108
P P+P S +PP PPP P
Sbjct: 246 PSPEPSSTMPPSTPPPQP 299
>TC8967 similar to UP|FXGA_HUMAN (P55316) Forkhead box protein G1A
(Forkhead-related protein FKHL2) (Transcription factor
BF-2) (Brain factor 2) (BF2) (HFK2), partial (6%)
Length = 1032
Score = 42.4 bits (98), Expect = 4e-04
Identities = 15/21 (71%), Positives = 16/21 (75%)
Frame = +2
Query: 90 HPHPKPLSMIPPPPPPPPPPP 110
H P P S+ PPPPPPPPPPP
Sbjct: 104 HSSPNP*SLAPPPPPPPPPPP 166
Score = 38.1 bits (87), Expect = 0.008
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = +2
Query: 91 PHPKPLSMIPPPPPPPPPPP 110
P+P L+ PPPPPPPPPPP
Sbjct: 113 PNP*SLAPPPPPPPPPPPPP 172
>TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II , partial
(6%)
Length = 583
Score = 41.6 bits (96), Expect = 7e-04
Identities = 31/112 (27%), Positives = 52/112 (45%), Gaps = 1/112 (0%)
Frame = +3
Query: 112 GEDGAVVVIESIVADEDGLGCGVGNVSEEGSGIGAEDGNERVNGEGSEIGADNGNERVTG 171
G G ++ E++ + G G +V + +G+G +G+ V+GE + +G G
Sbjct: 72 GGGGEILGGETLEPELGGGELGDDDVGDSEAGLGRVEGSVFVDGEAEDAVVVDGEGEDQG 251
Query: 172 EGSGIGSVHGNERVTGE-GSGIGACNGNESVTGEGSGIGGVHGNERVAVEDV 222
G G+G E V GE G G G+E G G+G G + +AV ++
Sbjct: 252 FGVGVGGWVFGEEVGGEVAVGDGDMGGDEGGGGGGAGSGSLAEWGLIAVSEL 407
Score = 28.1 bits (61), Expect = 8.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 94 KPLSMIPPPPPPPPPPPA 111
+P S P P PPPPPP+
Sbjct: 388 RPHSASEPDPAPPPPPPS 335
>TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (12%)
Length = 588
Score = 40.4 bits (93), Expect = 0.002
Identities = 29/96 (30%), Positives = 39/96 (40%), Gaps = 10/96 (10%)
Frame = +3
Query: 885 TMGAYRSTYEFYVHPTRSQQYWEP----------TDYVKPLPPNVPKRKAGRPKRKRRRD 934
T+ YR TY +HP + W+ D V PP R GRP++KR
Sbjct: 15 TVATYRKTYSQTIHPIPDKSLWKELSEGDENAAKADQVLINPPK-SLRPPGRPRKKR--- 182
Query: 935 ATEQQVAGGSRTRLRRTYPDITCTRCGLTGHNSRGC 970
V R R++R + C+RC TGH C
Sbjct: 183 -----VRAEDRGRVKRV---VHCSRCNQTGHFRTTC 266
>AW720051
Length = 564
Score = 40.0 bits (92), Expect = 0.002
Identities = 21/58 (36%), Positives = 29/58 (49%), Gaps = 4/58 (6%)
Frame = +1
Query: 101 PPPPPPPPPPAGEDGAVVVIES----IVADEDGLGCGVGNVSEEGSGIGAEDGNERVN 154
PPPPPPP P + G+ + ES AD+ G G GV N +E + NE ++
Sbjct: 331 PPPPPPPEPQSSSGGSSTLTESTSKMFAADQSGGGGGVFNEAESFTTSSMSSTNEAID 504
>TC14287 similar to UP|Q9LEU7 (Q9LEU7) Serine/threonine protein kinase-like
protein (CBL-interacting protein kinase 5), partial
(75%)
Length = 1999
Score = 38.9 bits (89), Expect = 0.005
Identities = 14/21 (66%), Positives = 15/21 (70%)
Frame = +3
Query: 91 PHPKPLSMIPPPPPPPPPPPA 111
PH P PPPPPPPPPPP+
Sbjct: 321 PHLSP*LATPPPPPPPPPPPS 383
Score = 28.1 bits (61), Expect = 8.4
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +3
Query: 103 PPPPPPPPAGEDGAVVVIES 122
PPPPPPPP + +V E+
Sbjct: 348 PPPPPPPPPPPSSSTMVPEN 407
>TC7897 weakly similar to UP|ENL2_ARATH (Q9T076) Early nodulin-like protein
2 precursor (Phytocyanin-like protein), partial (31%)
Length = 1482
Score = 38.5 bits (88), Expect = 0.006
Identities = 38/125 (30%), Positives = 50/125 (39%), Gaps = 6/125 (4%)
Frame = -3
Query: 104 PPPPPPPAGEDGAVV---VIESIVADEDGLGCGVGNVSEEGSGIGAEDGNERVNGEGSEI 160
PP PAGE V E +V DEDG + G+ G DG+ +G
Sbjct: 865 PPAGESPAGEGEVAVGPPAGELLVGDEDGDSAPGAGEAAGGALTGDGDGDVDADGGVPAD 686
Query: 161 GADNGNERVTGEGS---GIGSVHGNERVTGEGSGIGACNGNESVTGEGSGIGGVHGNERV 217
G G GEG+ G+ + G+ V G+ G G G+ GEG GG G V
Sbjct: 685 GPPAGTGAPAGEGNTDGGVPAGEGSPAVGGDPDG-GLAFGD----GEGEDFGGGDGGGCV 521
Query: 218 AVEDV 222
D+
Sbjct: 520 PRGDI 506
>CB829112
Length = 234
Score = 38.1 bits (87), Expect = 0.008
Identities = 16/23 (69%), Positives = 17/23 (73%), Gaps = 2/23 (8%)
Frame = +3
Query: 91 PHPKPLSMIPP--PPPPPPPPPA 111
P P PL + PP PPPPPPPPPA
Sbjct: 75 PPPPPLLLPPPLLPPPPPPPPPA 143
>BP049447
Length = 478
Score = 38.1 bits (87), Expect = 0.008
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +3
Query: 77 AKLNSGEIEIYFEHPHPKPLSMIPPPPPPPPPPP 110
A++N + H H +P S +P PPPPPPPP
Sbjct: 147 ARINQLLPHHHHHHHHQQPFSNLPSRPPPPPPPP 248
>BP049972
Length = 496
Score = 38.1 bits (87), Expect = 0.008
Identities = 30/110 (27%), Positives = 44/110 (39%), Gaps = 18/110 (16%)
Frame = -1
Query: 879 YCHAWLTMGAYRSTYEFYVHPTRSQQYW-----EPTDYVKPLPP-----------NVPK- 921
+ + T+ YR TY +HP + +W E + K + N PK
Sbjct: 490 FTESCFTVATYRKTYSQTIHPIPDRSFWNELSIEDANANKAVEDANASQSIEIVINPPKS 311
Query: 922 -RKAGRPKRKRRRDATEQQVAGGSRTRLRRTYPDITCTRCGLTGHNSRGC 970
R GRP++KR V R R++R + C+RC TGH C
Sbjct: 310 LRPPGRPRKKR--------VRAEDRGRVKRV---VHCSRCNQTGHFRTTC 194
>TC14720 UP|Q9FSH3 (Q9FSH3) Ammonium transporter (AMT1.1), complete
Length = 1774
Score = 38.1 bits (87), Expect = 0.008
Identities = 37/113 (32%), Positives = 46/113 (39%), Gaps = 13/113 (11%)
Frame = -2
Query: 112 GEDGAVVVIESIVADEDGLGCGVG-----NVSEEGSGIGAEDGNER-------VNGEGSE 159
G G V E + ADE GC G + EE +G G ED E +G G+E
Sbjct: 459 GYGGDVFEAEEVAADEAVGGCAEGKGETEEIVEEAAGGGVEDVGEHDVHGVFGADGAGAE 280
Query: 160 IG-ADNGNERVTGEGSGIGSVHGNERVTGEGSGIGACNGNESVTGEGSGIGGV 211
G A+ E G +G+VH E GE +G E V E GGV
Sbjct: 279 HGEAELHREYEVGGEEEVGAVH-RECCVGE----SLTHGGELVADESGDCGGV 136
>CN825698
Length = 704
Score = 38.1 bits (87), Expect = 0.008
Identities = 14/20 (70%), Positives = 14/20 (70%)
Frame = +1
Query: 91 PHPKPLSMIPPPPPPPPPPP 110
P P P S PPPPPPPPPP
Sbjct: 103 PPPSPSSASAPPPPPPPPPP 162
Score = 35.8 bits (81), Expect = 0.040
Identities = 13/19 (68%), Positives = 13/19 (68%)
Frame = +1
Query: 91 PHPKPLSMIPPPPPPPPPP 109
P P S PPPPPPPPPP
Sbjct: 109 PSPSSASAPPPPPPPPPPP 165
Score = 30.8 bits (68), Expect = 1.3
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = +1
Query: 91 PHPKPLSMIPPPPPPPPPPPAGED 114
P P P PPPPPP PPP+ D
Sbjct: 133 PPPPP----PPPPPPSLPPPSDSD 192
>TC17263 homologue to UP|RUBB_PEA (P08927) RuBisCO subunit binding-protein
beta subunit, chloroplast precursor (60 kDa chaperonin
beta subunit) (CPN-60 beta), partial (28%)
Length = 622
Score = 38.1 bits (87), Expect = 0.008
Identities = 15/29 (51%), Positives = 17/29 (57%)
Frame = +3
Query: 88 FEHPHPKPLSMIPPPPPPPPPPPAGEDGA 116
+ P P P + PPPP PP P PA DGA
Sbjct: 87 WRQPLPAPATSPPPPPSPPSPSPAARDGA 173
>TC13588 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall structural
protein 1.8 precursor (GRP 1.8), partial (6%)
Length = 517
Score = 37.7 bits (86), Expect = 0.011
Identities = 14/20 (70%), Positives = 14/20 (70%)
Frame = -3
Query: 90 HPHPKPLSMIPPPPPPPPPP 109
H P P IPPPPPPPPPP
Sbjct: 446 HFPPPPCPSIPPPPPPPPPP 387
>AV780471
Length = 527
Score = 37.4 bits (85), Expect = 0.014
Identities = 15/25 (60%), Positives = 17/25 (68%), Gaps = 2/25 (8%)
Frame = +3
Query: 87 YFEHPH--PKPLSMIPPPPPPPPPP 109
+F H P PL + PPPPPPPPPP
Sbjct: 153 FFHHRKNPPPPLFLRPPPPPPPPPP 227
Score = 30.8 bits (68), Expect = 1.3
Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 4/31 (12%)
Frame = +3
Query: 84 IEIYFEHPHPKPLSMIPPP----PPPPPPPP 110
++ +F H P PPP PPPPPPPP
Sbjct: 141 VQSFFFHHRKNP----PPPLFLRPPPPPPPP 221
>TC14068 homologue to UP|Q9SLR7 (Q9SLR7) Thiamin biosynthetic enzyme,
partial (85%)
Length = 1494
Score = 33.1 bits (74), Expect(2) = 0.016
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +1
Query: 90 HPHPKPLSMIPPPPPPPPPPP 110
H H L PPP PPPPPPP
Sbjct: 28 HLHRSWLPPPPPPSPPPPPPP 90
Score = 32.3 bits (72), Expect = 0.44
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +1
Query: 90 HPHPKPLSMIPPPPPPPPPPP 110
H H + PPPPP PPPPP
Sbjct: 22 HTHLHRSWLPPPPPPSPPPPP 84
Score = 22.7 bits (47), Expect(2) = 0.016
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 103 PPPPPPPPAGEDGAVVVIESIVADE 127
P PPPP G + ESIV+ E
Sbjct: 213 PSPPPPYDLGSFKFAPIKESIVSRE 287
>TC13980 similar to UP|Q9SGT0 (Q9SGT0) T6H22.18 protein (F14J16.33), partial
(12%)
Length = 565
Score = 37.0 bits (84), Expect = 0.018
Identities = 13/21 (61%), Positives = 14/21 (65%)
Frame = +2
Query: 90 HPHPKPLSMIPPPPPPPPPPP 110
HP P+ PPPPPPPPP P
Sbjct: 398 HPPPQQQPFSPPPPPPPPPSP 460
Score = 36.2 bits (82), Expect = 0.031
Identities = 13/21 (61%), Positives = 14/21 (65%)
Frame = +2
Query: 91 PHPKPLSMIPPPPPPPPPPPA 111
PHP P PPPPPPPPP+
Sbjct: 395 PHPPPQQQPFSPPPPPPPPPS 457
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.139 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,041,454
Number of Sequences: 28460
Number of extensions: 504344
Number of successful extensions: 14696
Number of sequences better than 10.0: 447
Number of HSP's better than 10.0 without gapping: 5171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 9580
length of query: 1159
length of database: 4,897,600
effective HSP length: 100
effective length of query: 1059
effective length of database: 2,051,600
effective search space: 2172644400
effective search space used: 2172644400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0391.10