BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0387.5
(124 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089154 81 5e-17
TC10958 similar to PIR|T47938|T47938 RING finger protein - Arabi... 29 0.27
BG662003 28 0.46
BP047662 27 1.0
TC11564 homologue to GB|AAC82338.1|3941324|AF042799 suppressor o... 25 3.0
TC14161 UP|AAR29293 (AAR29293) ADP-ribosylation factor, complete 25 3.0
AU251853 25 3.0
TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyprolin... 25 3.9
AI967628 25 3.9
BP030497 25 3.9
TC18767 25 5.1
TC8163 UP|R11C_LOTJA (Q40193) Ras-related protein Rab11C, complete 25 5.1
AW720141 25 5.1
TC10481 similar to UP|O80644 (O80644) At2g39570 protein, partial... 25 5.1
TC16662 weakly similar to UP|O80843 (O80843) At2g45760 protein, ... 24 6.6
TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3,... 24 6.6
TC9151 24 6.6
TC12652 similar to UP|Q9FG87 (Q9FG87) Beta-ketoacyl-CoA synthase... 24 8.7
AV415539 24 8.7
>AU089154
Length = 582
Score = 81.3 bits (199), Expect = 5e-17
Identities = 40/42 (95%), Positives = 40/42 (95%)
Frame = +3
Query: 1 MEVAVELEDDLFFADLSKQLSLLIMDEDDDPLASCAVHPQSF 42
MEVAVELEDDLFFA LSKQLSLLIMDEDDDPLASCAVHPQ F
Sbjct: 84 MEVAVELEDDLFFAXLSKQLSLLIMDEDDDPLASCAVHPQVF 209
Score = 36.2 bits (82), Expect = 0.002
Identities = 16/18 (88%), Positives = 16/18 (88%)
Frame = +3
Query: 40 QSFSGAIHPPPQSAILYE 57
QSFSG I PPPQSAILYE
Sbjct: 519 QSFSGTIXPPPQSAILYE 572
>TC10958 similar to PIR|T47938|T47938 RING finger protein - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (38%)
Length = 779
Score = 28.9 bits (63), Expect = 0.27
Identities = 17/41 (41%), Positives = 20/41 (48%)
Frame = +1
Query: 39 PQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPRR 79
PQS SG P P+S L E LRR +G S + RR
Sbjct: 277 PQSSSGNSSPSPRSETLPEYPLRRWRRGARCACANSPRKRR 399
>BG662003
Length = 448
Score = 28.1 bits (61), Expect = 0.46
Identities = 16/69 (23%), Positives = 31/69 (44%)
Frame = +2
Query: 46 IHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHV 105
I PP++ ++K +P +T RR+ ++ TSS K++ R H+
Sbjct: 101 ISSPPRTLTRASVKCCTKAKDPMTLLPSATLTRRRAKRRPTSSRKKFRVSPSIRRAAHHL 280
Query: 106 PIKNPFKET 114
P ++ +T
Sbjct: 281 PARSARPKT 307
>BP047662
Length = 196
Score = 26.9 bits (58), Expect = 1.0
Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 2/28 (7%)
Frame = +2
Query: 90 AKYQKQSQDTRMISH--VPIKNPFKETN 115
A YQKQSQDT +++ PIK+ K N
Sbjct: 47 ANYQKQSQDTTIMASQAPPIKDSLKSRN 130
>TC11564 homologue to GB|AAC82338.1|3941324|AF042799 suppressor of white
apricot homolog 2 {Mus musculus;} , partial (3%)
Length = 428
Score = 25.4 bits (54), Expect = 3.0
Identities = 14/53 (26%), Positives = 26/53 (48%), Gaps = 1/53 (1%)
Frame = -1
Query: 73 QSTQPRRKQRKGRTSSYAKYQKQSQDTRMIS-HVPIKNPFKETNDRAYIDPSA 124
++ QP+ + K ++ K Q+Q Q + H P +P K+ Y+D S+
Sbjct: 392 RAIQPKLHEEKEDSNLQKKKQQQQQQQIVFHPHSPQSHPMKQNRFDPYVDMSS 234
>TC14161 UP|AAR29293 (AAR29293) ADP-ribosylation factor, complete
Length = 912
Score = 25.4 bits (54), Expect = 3.0
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -2
Query: 37 VHPQSFSGAIHPPPQSAILY 56
VHP S S A HP PQ LY
Sbjct: 434 VHPHSTSCATHPLPQQHGLY 375
>AU251853
Length = 286
Score = 25.4 bits (54), Expect = 3.0
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = +1
Query: 39 PQSFSGAIHPPPQSAILYEQVL 60
P SF+G I+PPP ++ + +L
Sbjct: 160 PLSFTGPINPPPATSTSHSDIL 225
>TC13043 similar to GB|BAB21544.1|12641847|AB022782 hydroxyproline-rich
glycoprotein {Arabidopsis thaliana;} , partial (6%)
Length = 678
Score = 25.0 bits (53), Expect = 3.9
Identities = 14/36 (38%), Positives = 17/36 (46%)
Frame = +2
Query: 70 FIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHV 105
F+ S R+K G TS A+Y K S SHV
Sbjct: 425 FLSSSVCKRKKGESGVTSKSARYAKSSSSHCGNSHV 532
>AI967628
Length = 468
Score = 25.0 bits (53), Expect = 3.9
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -2
Query: 2 EVAVELEDDLFFA-DLSKQLSLLIMDEDDDPLASCAV 37
E + + +++ F + +L L+L ++D +DD L+ C+V
Sbjct: 407 EASEKFDEEAFPSPNLKSHLNLALLDVEDDSLSMCSV 297
>BP030497
Length = 513
Score = 25.0 bits (53), Expect = 3.9
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = -1
Query: 14 ADLSKQLSLLIMDEDDDPLASCAVHP 39
++L L+L + D +DD L+ C+V P
Sbjct: 405 SNLQSHLNLALRDVEDDLLSMCSVAP 328
>TC18767
Length = 1004
Score = 24.6 bits (52), Expect = 5.1
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +3
Query: 55 LYEQVLRRQSKGTGVFIPQSTQPRR 79
LY++VLRR+ TG +P Q R
Sbjct: 828 LYQEVLRREGPRTGTEVPCMMQSPR 902
>TC8163 UP|R11C_LOTJA (Q40193) Ras-related protein Rab11C, complete
Length = 1193
Score = 24.6 bits (52), Expect = 5.1
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -1
Query: 71 IPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMIS 103
+PQ TQP KG SY Y++ + + +IS
Sbjct: 572 VPQFTQPPLDIVKGLPLSYVIYKESTNSSSVIS 474
>AW720141
Length = 505
Score = 24.6 bits (52), Expect = 5.1
Identities = 19/61 (31%), Positives = 26/61 (42%), Gaps = 5/61 (8%)
Frame = -3
Query: 62 RQSKGTGVFIPQSTQPRRKQRKGRTSSY---AKYQKQS--QDTRMISHVPIKNPFKETND 116
+QS G P+ R Q GRT+S + S D R +SH P NP++
Sbjct: 290 KQSAWRGQRTPEPRN*RNSQAPGRTTSLNS*TSWLGPS*VADPRRLSHEPRANPWRGRKT 111
Query: 117 R 117
R
Sbjct: 110 R 108
>TC10481 similar to UP|O80644 (O80644) At2g39570 protein, partial (31%)
Length = 1091
Score = 24.6 bits (52), Expect = 5.1
Identities = 16/42 (38%), Positives = 21/42 (49%)
Frame = +3
Query: 76 QPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETNDR 117
+P R R+G SSY Y SQD SH+ + +E DR
Sbjct: 453 EPGRVVRQGAASSYI*YHSCSQDA---SHLHLFG*NREACDR 569
>TC16662 weakly similar to UP|O80843 (O80843) At2g45760 protein, partial
(13%)
Length = 816
Score = 24.3 bits (51), Expect = 6.6
Identities = 13/51 (25%), Positives = 20/51 (38%), Gaps = 1/51 (1%)
Frame = -2
Query: 67 TGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHV-PIKNPFKETND 116
T + +PQ RTS K + R SH P P+++ N+
Sbjct: 620 TSIVVPQKGNTSNNTASNRTSRNLKSTRTHHHLRRCSHTFPRPQPYRKINN 468
>TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3, P1 clone:
MIG10, partial (18%)
Length = 1118
Score = 24.3 bits (51), Expect = 6.6
Identities = 11/43 (25%), Positives = 23/43 (52%)
Frame = +2
Query: 74 STQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETND 116
+T PR+++RKG T + + Q + + + H P ++ +D
Sbjct: 287 TTLPRKQERKGETIHQTEAENQLPNRKRL-HARSTKPSRQASD 412
>TC9151
Length = 1236
Score = 24.3 bits (51), Expect = 6.6
Identities = 18/59 (30%), Positives = 26/59 (43%)
Frame = +1
Query: 34 SCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKY 92
S + P FSG+I PQ+A L LR +P +P+R +R G + Y
Sbjct: 187 SVPIPPNPFSGSILRRPQTASLLS--LRS--------VPPCLRPKRNRRFGSAREWNLY 333
>TC12652 similar to UP|Q9FG87 (Q9FG87) Beta-ketoacyl-CoA synthase
(AT5g43760/MQD19_11), partial (8%)
Length = 461
Score = 23.9 bits (50), Expect = 8.7
Identities = 14/46 (30%), Positives = 19/46 (40%)
Frame = +1
Query: 49 PPQSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQK 94
P ++ + R + GTG +S Q RRK S KY K
Sbjct: 196 PDSKPLMEDHKNRTKDSGTGTISVESPQERRKLPNFLLSVKLKYVK 333
>AV415539
Length = 419
Score = 23.9 bits (50), Expect = 8.7
Identities = 10/26 (38%), Positives = 13/26 (49%)
Frame = +3
Query: 31 PLASCAVHPQSFSGAIHPPPQSAILY 56
PL AV P +HPPP I++
Sbjct: 327 PLLPLAVPPHGEDRVLHPPPP*IIIF 404
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.131 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,940,080
Number of Sequences: 28460
Number of extensions: 24744
Number of successful extensions: 139
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of query: 124
length of database: 4,897,600
effective HSP length: 79
effective length of query: 45
effective length of database: 2,649,260
effective search space: 119216700
effective search space used: 119216700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0387.5