BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0383.7
(316 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP051903 61 3e-10
BP078889 60 5e-10
TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related prote... 57 5e-09
BG662008 56 7e-09
TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1... 47 3e-06
AV417836 42 2e-04
TC15363 similar to UP|Q84LL8 (Q84LL8) Salt tolerance protein 4, ... 40 5e-04
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 38 0.003
TC11058 36 0.010
TC14530 similar to UP|P82277 (P82277) Plastid-specific 30S ribos... 35 0.022
AV411398 35 0.022
BP036697 34 0.028
BE122637 34 0.028
TC18938 similar to UP|AAS00039 (AAS00039) Splicing factor-like p... 33 0.048
AV775543 33 0.048
AI967582 33 0.048
TC13693 similar to PIR|T00933|T00933 RNA-binding protein homolog... 32 0.14
TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 32 0.14
AV409162 32 0.14
AV415981 31 0.31
>BP051903
Length = 342
Score = 60.8 bits (146), Expect = 3e-10
Identities = 31/75 (41%), Positives = 47/75 (62%)
Frame = +2
Query: 39 VDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLNG 98
VDG+ D Y+ +R +F ++G + VF+QR+RK GR RF F+RF + A +AI LNG
Sbjct: 68 VDGIPDRTDYYQIRGLFAQFGRVVNVFVQRQRKIGRLFRFGFVRFASLEVASKAIDFLNG 247
Query: 99 SWFFESFLMVNRARY 113
++ L V+ AR+
Sbjct: 248 FRLGDAALSVSMARF 292
>BP078889
Length = 474
Score = 60.1 bits (144), Expect = 5e-10
Identities = 32/92 (34%), Positives = 51/92 (54%)
Frame = +1
Query: 22 GVDRQQWRTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFI 81
G++R ++ T FVDG+ + + YH LR +F +G + +F+QR+ K GR+ RF F+
Sbjct: 199 GLNRNNGGERRRKPTPFVDGIEEAIEYHHLRGLFALFGRISNLFVQRKHKFGRRFRFGFV 378
Query: 82 RFKYEQDACEAIRSLNGSWFFESFLMVNRARY 113
RF E+ A L+G + L V AR+
Sbjct: 379 RFFSEEQAAAVAFVLDGLRVGGTALSVAPARF 474
>TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related protein, partial
(5%)
Length = 481
Score = 56.6 bits (135), Expect = 5e-09
Identities = 26/64 (40%), Positives = 40/64 (61%)
Frame = +1
Query: 35 FTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
FT+F+DG+S+ +Y+ +R +F + G L VFLQ++R+ R RF F+R+ A AI
Sbjct: 277 FTLFIDGISEPTNYYHIRGLFGQIGSLLNVFLQKQRRIRRNFRFGFVRYSTRDVASMAIN 456
Query: 95 SLNG 98
NG
Sbjct: 457 LFNG 468
>BG662008
Length = 457
Score = 56.2 bits (134), Expect = 7e-09
Identities = 27/59 (45%), Positives = 37/59 (61%)
Frame = +3
Query: 36 TIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
T+FVDG+SD V Y +R +F G L VF+QR+R+ GR RF F+RF + A +R
Sbjct: 102 TLFVDGISDFVDYSQIRGLFSHIGSLLNVFVQRQRRIGRAFRFGFVRFPSVELASSMVR 278
>TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1_4
{Arabidopsis thaliana;}, partial (55%)
Length = 1276
Score = 47.4 bits (111), Expect = 3e-06
Identities = 22/79 (27%), Positives = 44/79 (54%)
Frame = +2
Query: 35 FTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
+++ V ++ + DL +F++YG + +F+ + R+ G FAF+R+KY +A +A+
Sbjct: 131 YSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAVD 310
Query: 95 SLNGSWFFESFLMVNRARY 113
L+G + V A+Y
Sbjct: 311 RLDGRMVDGREITVQFAKY 367
>AV417836
Length = 429
Score = 41.6 bits (96), Expect = 2e-04
Identities = 19/48 (39%), Positives = 28/48 (57%)
Frame = +1
Query: 50 DLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLN 97
DLR FERYG + V+L + G F F+++++ +DA EA LN
Sbjct: 166 DLRGPFERYGPVKDVYLPKNYYTGEPRGFGFVKYRFGEDAAEAKHHLN 309
>TC15363 similar to UP|Q84LL8 (Q84LL8) Salt tolerance protein 4, partial
(79%)
Length = 1163
Score = 40.0 bits (92), Expect = 5e-04
Identities = 21/63 (33%), Positives = 32/63 (50%)
Frame = +1
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
I V GV + DL+ F YG + + L R+ G +A I ++ ++A AI +L
Sbjct: 388 ILVTGVHEEAQEDDLQNAFGEYGEIKNLHLNLDRRTGFVKGYALIEYERAEEAKNAIENL 567
Query: 97 NGS 99
NGS
Sbjct: 568 NGS 576
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 37.7 bits (86), Expect = 0.003
Identities = 22/62 (35%), Positives = 33/62 (52%)
Frame = +1
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
++V + VS DL F +GV+ V++ RR +AFI F +DA +AIR L
Sbjct: 94 VYVGNLDSRVSERDLEDEFRVFGVIRSVWVARR-----PPGYAFIDFDDRRDAQDAIREL 258
Query: 97 NG 98
+G
Sbjct: 259 DG 264
>TC11058
Length = 539
Score = 35.8 bits (81), Expect = 0.010
Identities = 20/52 (38%), Positives = 33/52 (63%)
Frame = +1
Query: 50 DLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLNGSWF 101
D+R +F R+G + V L + KQGR+ +AFI + E++ +A SL+G+ F
Sbjct: 19 DVRYLFARFGNVASVRLLQDLKQGRRRAYAFISYLSERER-DAAMSLHGTEF 171
>TC14530 similar to UP|P82277 (P82277) Plastid-specific 30S ribosomal
protein 2, chloroplast precursor (PSRP-2), partial (68%)
Length = 1104
Score = 34.7 bits (78), Expect = 0.022
Identities = 23/93 (24%), Positives = 41/93 (43%)
Frame = +2
Query: 7 ARAWPVSRPEVVGSHGVDRQQWRTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFL 66
AR + RP V Q D++ ++V + ++ +L + + + + +
Sbjct: 158 ARPLRLRRPFAVAEQAAATQS-EADRR---LYVGNIPRTLTNDELANIVQEHAAVEKAEV 325
Query: 67 QRRRKQGRKSRFAFIRFKYEQDACEAIRSLNGS 99
+ GR RFAF+ K +DA AI LNG+
Sbjct: 326 MYDKYSGRSRRFAFVTVKTVEDANAAIEKLNGT 424
>AV411398
Length = 427
Score = 34.7 bits (78), Expect = 0.022
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 4/75 (5%)
Frame = +3
Query: 42 VSDNVSY----HDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLN 97
V+ NV + D+R +FERYG + V L K R AF+ ++A EA+ L
Sbjct: 87 VAQNVPWTSTPEDVRSLFERYGTVLEVELSMYNKT-RSRGLAFVEMSSPEEALEALNKLE 263
Query: 98 GSWFFESFLMVNRAR 112
F L +N AR
Sbjct: 264 SYEFEGRVLKLNYAR 308
>BP036697
Length = 578
Score = 34.3 bits (77), Expect = 0.028
Identities = 37/152 (24%), Positives = 66/152 (43%), Gaps = 3/152 (1%)
Frame = +3
Query: 36 TIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRS 95
T++V + ++ ++ +F +YG + + L + R +AF+ F+ QDA +AIR
Sbjct: 99 TVYVGNLPGDIREREVEDLFYKYGNITHIDL---KVPPRPPGYAFVEFEDTQDAEDAIRG 269
Query: 96 LNGSWFFESFLMVNRARYDYTNRRNVTNRHSKVWKQKFVGEKQAFGQETVV--SEGRKQV 153
+G F L V A + R N ++R ++ G V SE R V
Sbjct: 270 RDGYDFDGHRLRVELA---HGGRGNSSSR------DRYSSHSSGRGGRGVSRRSEYRVLV 422
Query: 154 GQQPAKQVWRE-KCRTQEVHDMIFHTMVEDQR 184
P+ W++ K ++ D+ F + D R
Sbjct: 423 SGLPSSASWQDLKDHMRKAGDVCFSQVFHDGR 518
>BE122637
Length = 332
Score = 34.3 bits (77), Expect = 0.028
Identities = 23/86 (26%), Positives = 40/86 (45%), Gaps = 5/86 (5%)
Frame = +2
Query: 50 DLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLNGSWFFESFLMV- 108
++R FER+G + V++ + G FAF++F DA EA +N F + V
Sbjct: 68 EIRVPFERFGPVRDVYIPKDYYSGEPRGFAFVQFVDPYDASEAQYHMNRQIFAGREITVV 247
Query: 109 ----NRARYDYTNRRNVTNRHSKVWK 130
R R + R ++ + ++WK
Sbjct: 248 VASETRKRPEEMRHRTRSS*NRQLWK 325
>TC18938 similar to UP|AAS00039 (AAS00039) Splicing factor-like protein,
partial (18%)
Length = 473
Score = 33.5 bits (75), Expect = 0.048
Identities = 23/70 (32%), Positives = 36/70 (50%)
Frame = +3
Query: 32 KKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACE 91
+K+ +IF + + R+F RYG + V + KS FAFI + E+DA
Sbjct: 210 RKMRSIFCGNFEYDARQSESERLFRRYGKVDRVDM--------KSGFAFIYMEDERDADA 365
Query: 92 AIRSLNGSWF 101
AIR+L+ + F
Sbjct: 366 AIRALDRAEF 395
>AV775543
Length = 355
Score = 33.5 bits (75), Expect = 0.048
Identities = 19/48 (39%), Positives = 28/48 (57%)
Frame = +1
Query: 50 DLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLN 97
+L R+F +YG R + KS FAF+ F+ E+D EAIR+L+
Sbjct: 10 ELERLFSKYG--------RIERLDMKSGFAFVYFEDERDGEEAIRALD 129
>AI967582
Length = 356
Score = 33.5 bits (75), Expect = 0.048
Identities = 15/35 (42%), Positives = 21/35 (59%)
Frame = +2
Query: 64 VFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLNG 98
VF+QR+ + GR+ F F+ F DA A+ LNG
Sbjct: 20 VFVQRKHEVGRRFHFGFVHFLSWSDAXTAVARLNG 124
>TC13693 similar to PIR|T00933|T00933 RNA-binding protein homolog At2g42240
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(53%)
Length = 662
Score = 32.0 bits (71), Expect = 0.14
Identities = 27/109 (24%), Positives = 48/109 (43%)
Frame = +3
Query: 33 KVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEA 92
+V T+FV G+ ++V ++ +F + L R FAF F +Q A A
Sbjct: 192 EVRTLFVAGLPEDVKPREIYNLFREFPGYESSHL--RSPTNSSQPFAFAVFMNQQSAVMA 365
Query: 93 IRSLNGSWFFESFLMVNRARYDYTNRRNVTNRHSKVWKQKFVGEKQAFG 141
+ +LNG F L N Y + N + +++ ++ +K+A G
Sbjct: 366 MHALNGMVF---DLEKNSTLYIDLAKSNSRSNRTRIDDERGGSDKKARG 503
>TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (77%)
Length = 1576
Score = 32.0 bits (71), Expect = 0.14
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 1/69 (1%)
Frame = +3
Query: 31 DKKVFTIFVDGVSDNVSYHDLRRMFE-RYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDA 89
D +TIFV ++ +V+ + L +F RY + G + R R + F+RF E +
Sbjct: 603 DSPDYTIFVGDLAADVTDYHLTEVFRTRYNSVKGAKVVIDRLTSRTKGYGFVRFADESEQ 782
Query: 90 CEAIRSLNG 98
A+ + G
Sbjct: 783 VRAMTEMQG 809
>AV409162
Length = 426
Score = 32.0 bits (71), Expect = 0.14
Identities = 16/62 (25%), Positives = 34/62 (54%)
Frame = +3
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
+F+ + NVS ++ +F YG + + + R +Q K AF++++ + A A+ ++
Sbjct: 234 LFIGMLPKNVSEVEVSALFSNYGTIKDLQILRGSQQTSKG-CAFLKYETKDQALAALEAI 410
Query: 97 NG 98
NG
Sbjct: 411 NG 416
>AV415981
Length = 333
Score = 30.8 bits (68), Expect = 0.31
Identities = 17/55 (30%), Positives = 29/55 (51%)
Frame = +3
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACE 91
+FV ++ DL +F R+G + + R +K G +AFI F+ ++ ACE
Sbjct: 60 LFVCKLNPVTEDEDLHTIFSRFGTVSSADIIRDQKTGDSLCYAFIEFE-DKQACE 221
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,358,192
Number of Sequences: 28460
Number of extensions: 66851
Number of successful extensions: 456
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 456
length of query: 316
length of database: 4,897,600
effective HSP length: 90
effective length of query: 226
effective length of database: 2,336,200
effective search space: 527981200
effective search space used: 527981200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0383.7