BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0359.3
(1572 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 278 4e-80
BP084332 166 2e-41
AV780009 115 5e-26
BP052632 105 4e-23
BP075943 76 5e-14
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 44 1e-11
BP063694 68 1e-11
AV427921 67 3e-11
AV766665 63 4e-10
AU088980 63 4e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 59 5e-09
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 57 2e-08
AV766088 54 3e-07
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 53 3e-07
BP033602 52 6e-07
AV424544 52 7e-07
BP057233 50 2e-06
BP043850 49 6e-06
BP085942 40 9e-05
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 35 1e-04
>BP066094
Length = 532
Score = 278 bits (711), Expect(3) = 4e-80
Identities = 136/149 (91%), Positives = 142/149 (95%)
Frame = +2
Query: 1187 ARLEAIRLLISFSVNHNIVLHQMDVKSAFLNGYISEEVYVHQPPGFEDERKPDHVFKLKK 1246
++ EAIRLLISFSVNHNI+LHQM+VKSAFLNGYISEEVYVHQPPG EDE+ DH+FKLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1247 SLYGLKQAPRAWYERLSSFLLENEFVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQ 1306
SLYGLKQAPRAWYERLSSFLLENE VRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSAN
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1307 SLCKEFSEMMQAEFEMSMMGELKYFLGIQ 1335
SLCKEFSEMMQAEFEM MMGELKYFLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 37.4 bits (85), Expect(3) = 4e-80
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3
Query: 1171 YSQQEGIDYTETFAPVAR 1188
YSQQ GIDYTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 4e-80
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1161 RNKARLVAQG 1170
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP084332
Length = 368
Score = 166 bits (421), Expect(2) = 2e-41
Identities = 82/82 (100%), Positives = 82/82 (100%)
Frame = +2
Query: 1491 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 1550
QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESNIPIYCDNTAAISLSKNPILHS 211
Query: 1551 RAKHIEVKYHFIRDYVQKGVLL 1572
RAKHIEVKYHFIRDYVQKGVLL
Sbjct: 212 RAKHIEVKYHFIRDYVQKGVLL 277
Score = 21.6 bits (44), Expect(2) = 2e-41
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 1476 CQFLGSNL 1483
CQFLGSNL
Sbjct: 4 CQFLGSNL 27
>AV780009
Length = 529
Score = 115 bits (288), Expect = 5e-26
Identities = 58/150 (38%), Positives = 93/150 (61%), Gaps = 6/150 (4%)
Frame = -1
Query: 1428 AVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTFGNCQFLGSNLVSWA 1487
A R+LRY+KG GL + S KL Y D+D+AG R+S G FLG++L+SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 1488 SKRQSTIALSTAEAEY--ISAAICSTQMLWMKHQLEDYQILESN----IPIYCDNTAAIS 1541
+K+Q+T++ S++EAEY ++A +C Q W+ + +Q L+ N +P++CDN +A+
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQ--WLSYL---FQFLKLNVPLPVPLFCDNQSALH 182
Query: 1542 LSKNPILHSRAKHIEVKYHFIRDYVQKGVL 1571
++ NP H R KHIE+ H +R +Q G++
Sbjct: 181 IAHNPTFHERTKHIELDCHVVRAKLQAGLI 92
>BP052632
Length = 489
Score = 105 bits (263), Expect = 4e-23
Identities = 50/114 (43%), Positives = 71/114 (61%)
Frame = -3
Query: 932 KTPYELWKNIKPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSDRSKGFRFYNT 991
KTPYELW+ +P +SYFHPFGC ++LNT D + KFD K K +LLGYS SK + +N+
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 992 DAKTIEESIHVRFDDKLDSDQSKLVEKFADLSINVSDKGKAPEEVEPEEDEPEE 1045
+ +EESI+V+FDD +L + F +L ++ D+ E +P E EE
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEKDFLNLDLSDQDEEVPTNEGQPSETSHEE 146
>BP075943
Length = 547
Score = 75.9 bits (185), Expect = 5e-14
Identities = 45/159 (28%), Positives = 78/159 (48%), Gaps = 2/159 (1%)
Frame = -3
Query: 1294 IYVDDIIFGSANQSLCKEFSEMMQAEFEMSMMGELKYFLGIQVDQTPEGTYIHQSKYTKE 1353
+YVDD++ + + + +F + +GE KYFLG+++ ++ G ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1354 LLKKFNMLESTVAKTPMHPTCILEKEDKSGKVCQKL--YRGMIGSLLYLTASRPDILFSV 1411
L+ + H T +G + YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHF-GFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAV 159
Query: 1412 HLCARFQSDPRETHLTAVKRILRYLKGTTNLGLMYKKTS 1450
+ ++F S P + H + L+YL G+ GL Y +S
Sbjct: 158 NQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASS 42
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 44.3 bits (103), Expect(2) = 1e-11
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +2
Query: 1532 IYCDNTAAISLSKNPILHSRAKHIEVKYHF 1561
+YCDN AI L +N HSR+KHI+V+YH+
Sbjct: 173 LYCDNQGAIYLGQNSTFHSRSKHIDVRYHW 262
Score = 43.5 bits (101), Expect(2) = 1e-11
Identities = 19/39 (48%), Positives = 29/39 (73%)
Frame = +3
Query: 1484 VSWASKRQSTIALSTAEAEYISAAICSTQMLWMKHQLED 1522
V+W S+ Q +ALSTAEAE+I+A ++LWMK+ L++
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQN 143
>BP063694
Length = 511
Score = 67.8 bits (164), Expect = 1e-11
Identities = 44/122 (36%), Positives = 61/122 (49%), Gaps = 3/122 (2%)
Frame = -2
Query: 720 VWHRRLGHASMRKISQLSK---LNLVRGLPNLKFASDALCEACQKGKFTKVPFNAKNVVS 776
+WH RLGH + I QL K L++ LP C +CQ K ++ F+ N
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNNK-R 199
Query: 777 TSRPLELLHIDLFGPVKTESIGGKRYGMVIVDDYSRWTWVKFLTRKDESHAVFSTFIAQV 836
S L+L+H DL GP SI G Y ++ VDD+SR+TW L RK + V F +
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFM 19
Query: 837 QN 838
+N
Sbjct: 18 EN 13
>AV427921
Length = 284
Score = 66.6 bits (161), Expect = 3e-11
Identities = 33/79 (41%), Positives = 45/79 (56%)
Frame = +1
Query: 878 TPQQNGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIPNRISVRPILNKTPYEL 937
TPQQNGV ERKNRT+ M R++L +G+ K F EAV + +I NR + N P E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 938 WKNIKPNISYFHPFGCVCY 956
W +P + +F C+ Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>AV766665
Length = 601
Score = 62.8 bits (151), Expect = 4e-10
Identities = 32/76 (42%), Positives = 44/76 (57%)
Frame = +3
Query: 1427 TAVKRILRYLKGTTNLGLMYKKTSEYKLSGYCDADYAGDRTERKSTFGNCQFLGSNLVSW 1486
T RI YLK + G +++K + + G+ ADY G +R ST G FL NLV+W
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTW 539
Query: 1487 ASKRQSTIALSTAEAE 1502
SK+Q+ IA S+ EAE
Sbjct: 540 RSKQQNIIARSSGEAE 587
>AU088980
Length = 360
Score = 62.8 bits (151), Expect = 4e-10
Identities = 29/105 (27%), Positives = 51/105 (47%)
Frame = +2
Query: 882 NGVVERKNRTLQEMARTMLQETGMAKHFWAEAVNTACYIPNRISVRPILNKTPYELWKNI 941
N +VERK+R + + R ++ + + K+ W AV A Y+ N + + + PY+ N
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 942 KPNISYFHPFGCVCYVLNTKDRLHKFDAKSSKCLLLGYSDRSKGF 986
P + FG +CY K D ++ KC+ LG+ + G+
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 59.3 bits (142), Expect = 5e-09
Identities = 37/145 (25%), Positives = 71/145 (48%), Gaps = 9/145 (6%)
Frame = +2
Query: 1264 SFLLENEFVRGKVDTTLFCKTYKD-DILIVQIYVDDIIFGSANQSLCKEFSEMMQAEFEM 1322
SF++ + R D + K + D D +I+ +YVDD++ N+ +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1323 SMMGELKYFLGIQV--DQTPEGTYIHQSKYTKELLKKFNMLESTVAKTP------MHPTC 1374
+G LG+Q+ D+ ++ Q Y +++L++FNM + TP + +
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1375 ILEKEDKSGKVCQKLYRGMIGSLLY 1399
I E + ++ + Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 57.4 bits (137), Expect = 2e-08
Identities = 22/58 (37%), Positives = 38/58 (64%), Gaps = 1/58 (1%)
Frame = +1
Query: 1515 WMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKGVL 1571
W+ + L+D ++ E +YCDN +A ++ NP+ H R KHIE+ H +R+ +QKG++
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLI 177
>AV766088
Length = 501
Score = 53.5 bits (127), Expect = 3e-07
Identities = 36/108 (33%), Positives = 58/108 (53%), Gaps = 1/108 (0%)
Frame = -3
Query: 718 QWVWHRRLGHASMRKISQLSKLNLVRGLPNLKFASDALCEACQKGKFTKVPFNAKNVVST 777
Q +WH RLGH S + L + N L ++ +CE+C GK ++PF + N V T
Sbjct: 313 QSLWHSRLGHPSSSALRYL-RSNKFISYELLNYSP--VCESCVFGKHVRLPFVSSNNV-T 146
Query: 778 SRPLELLHIDLF-GPVKTESIGGKRYGMVIVDDYSRWTWVKFLTRKDE 824
P ++LH DL+ PV S G R+ ++ +DD++ + W L+ K +
Sbjct: 145 VMPFDILHSDLWTSPVL--SSAGHRFYVLFLDDFTDFLWTFPLSNKSQ 8
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 53.1 bits (126), Expect = 3e-07
Identities = 36/99 (36%), Positives = 49/99 (49%), Gaps = 1/99 (1%)
Frame = -1
Query: 1347 QSKYTKELLKKFNMLESTVAKTPMHPTCILEKEDKSGK-VCQKLYRGMIGSLLYLTASRP 1405
Q Y ++LKKF M S T + I GK V Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1406 DILFSVHLCARFQSDPRETHLTAVKRILRYLKGTTNLGL 1444
DI+ V L +RF +P + H +R LRY+KGT G+
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>BP033602
Length = 533
Score = 52.4 bits (124), Expect = 6e-07
Identities = 30/99 (30%), Positives = 53/99 (53%)
Frame = +3
Query: 713 SVNEEQWVWHRRLGHASMRKISQLSKLNLVRGLPNLKFASDALCEACQKGKFTKVPFNAK 772
SV ++ +WH RLGH + + + L +L + + S CE+C K + P+ ++
Sbjct: 255 SVRDQIMLWHNRLGHPNFQYLRHLFP-DLFKNVN----CSSLECESCVLAKNQRAPYYSQ 419
Query: 773 NVVSTSRPLELLHIDLFGPVKTESIGGKRYGMVIVDDYS 811
SRP L+H D++GP K + GKR+ + +DD++
Sbjct: 420 PY-HASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV424544
Length = 276
Score = 52.0 bits (123), Expect = 7e-07
Identities = 26/90 (28%), Positives = 49/90 (53%)
Frame = +3
Query: 1261 RLSSFLLENEFVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANQSLCKEFSEMMQAEF 1320
+LSS+L +++ D +LF K ++ +YVDD+I + + + + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 1321 EMSMMGELKYFLGIQVDQTPEGTYIHQSKY 1350
+ +G LKYFLG++V ++ G ++ Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>BP057233
Length = 473
Score = 50.4 bits (119), Expect = 2e-06
Identities = 19/35 (54%), Positives = 29/35 (82%)
Frame = -2
Query: 1532 IYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYV 1566
++CDN +A +L+ NP+LH+R+KHIE+ H+IRD V
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQV 341
>BP043850
Length = 515
Score = 48.9 bits (115), Expect = 6e-06
Identities = 20/62 (32%), Positives = 39/62 (62%), Gaps = 1/62 (1%)
Frame = -1
Query: 1511 TQMLWMKHQLEDYQILESN-IPIYCDNTAAISLSKNPILHSRAKHIEVKYHFIRDYVQKG 1569
++++W++ L + L+S P++ DNT+AI ++ NP+ H +HIEV H +R+ +
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 1570 VL 1571
V+
Sbjct: 335 VI 330
>BP085942
Length = 208
Score = 40.0 bits (92), Expect(2) = 9e-05
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +3
Query: 818 FLTRKDESHAVFSTFIAQVQNEKACRIVRVRSDHG 852
++ KDE+H F+ FI QVQ E ++ VRSDHG
Sbjct: 39 WILHKDETHTRFTNFITQVQKEFQTSVITVRSDHG 143
Score = 24.3 bits (51), Expect(2) = 9e-05
Identities = 10/17 (58%), Positives = 11/17 (63%)
Frame = +1
Query: 852 GGEFENDKFESLFDSYG 868
GGEFEN E F+S G
Sbjct: 142 GGEFENKALEEWFNSQG 192
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 35.4 bits (80), Expect(2) = 1e-04
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +2
Query: 1535 DNTAAISLSKNPILHSRAKHIE 1556
DN +AI L+KNP+ H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
Score = 28.5 bits (62), Expect(2) = 1e-04
Identities = 11/16 (68%), Positives = 13/16 (80%)
Frame = +3
Query: 1554 HIEVKYHFIRDYVQKG 1569
+IE KYHF+RD V KG
Sbjct: 69 NIETKYHFLRDQVTKG 116
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.332 0.142 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,847,117
Number of Sequences: 28460
Number of extensions: 351142
Number of successful extensions: 2194
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 2156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2183
length of query: 1572
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1470
effective length of database: 1,994,680
effective search space: 2932179600
effective search space used: 2932179600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0359.3