BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.4
(142 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 66 1e-14
AV768362 73 2e-14
TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 67 2e-12
AV777119 66 2e-12
TC19496 similar to UP|Q8LPQ6 (Q8LPQ6) At4g25450/M7J2_180, partia... 66 2e-12
BP055390 58 1e-11
BG662257 44 1e-08
TC8591 similar to UP|O80680 (O80680) At2g41040 protein, partial ... 27 1.8
>TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(9%)
Length = 712
Score = 65.9 bits (159), Expect(2) = 1e-14
Identities = 31/68 (45%), Positives = 50/68 (72%)
Frame = +2
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
+ VQ+ALD+ M+ RTT+++AHRLSTI+ AD++ VL+ G + E G H+ L + ++G YA+
Sbjct: 215 RVVQDALDKVMVNRTTVIVAHRLSTIKNADVITVLKNGVIVEKGRHETLINI-KDGYYAS 391
Query: 113 LVKMQEMA 120
LV++ A
Sbjct: 392 LVQLHTTA 415
Score = 28.1 bits (61), Expect(2) = 1e-14
Identities = 14/43 (32%), Positives = 23/43 (52%)
Frame = +1
Query: 17 TLWKAKTENSDSKGNAEKTSNSSPR*GNKCIGL*VRKAVQEAL 59
T+W A+T S N + + +P *G+KC *VR++ +
Sbjct: 106 TIWWAETTGSYCPSNNKNSKYIAPG*GHKCTRR*VRESCSRCI 234
>AV768362
Length = 541
Score = 73.2 bits (178), Expect = 2e-14
Identities = 38/63 (60%), Positives = 47/63 (74%)
Frame = -2
Query: 55 VQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANLV 114
VQ+A+D M GRT LVIAHRLST++ A+ V V+ G V+E GTHDEL S +NGVY+ LV
Sbjct: 522 VQDAMDSIMKGRTVLVIAHRLSTVKTANTVVVISDGQVAEKGTHDELLS--QNGVYSALV 349
Query: 115 KMQ 117
K Q
Sbjct: 348 KRQ 340
>TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(7%)
Length = 552
Score = 66.6 bits (161), Expect = 2e-12
Identities = 32/68 (47%), Positives = 50/68 (73%)
Frame = +3
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
+ VQ+ALD+ M+ RTT+++AHRLSTI+ AD++ VL+ G + E G H+ L S ++G YA+
Sbjct: 66 RVVQDALDKVMVNRTTVIVAHRLSTIKSADVIIVLKNGVIVEKGRHETLISI-KDGYYAS 242
Query: 113 LVKMQEMA 120
LV++ A
Sbjct: 243 LVQLHTTA 266
>AV777119
Length = 447
Score = 66.2 bits (160), Expect = 2e-12
Identities = 30/55 (54%), Positives = 42/55 (75%)
Frame = -1
Query: 63 MIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANLVKMQ 117
M+GRT++V+AHRLSTI+ DL+AVL +G V E G+H L +KG +G Y +LV +Q
Sbjct: 444 MVGRTSVVVAHRLSTIQNCDLIAVLDKGRVVEKGSHSNLLAKGPSGAYYSLVSLQ 280
>TC19496 similar to UP|Q8LPQ6 (Q8LPQ6) At4g25450/M7J2_180, partial (10%)
Length = 571
Score = 66.2 bits (160), Expect = 2e-12
Identities = 36/70 (51%), Positives = 48/70 (68%)
Frame = +3
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
+ VQ+AL+ M GRTTLVIAHRLST++ A +A+ G ++E GTH EL +K G YA+
Sbjct: 18 RLVQDALNHLMKGRTTLVIAHRLSTVQNAYQIALCSDGRIAEQGTHFELLAK--KGQYAS 191
Query: 113 LVKMQEMAHE 122
LV Q +A E
Sbjct: 192 LVGTQRLAFE 221
>BP055390
Length = 488
Score = 57.8 bits (138), Expect(2) = 1e-11
Identities = 28/49 (57%), Positives = 36/49 (73%)
Frame = +3
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDEL 101
K VQEAL+ M GRT ++IAHRLST+ AD++AV++ G V E GTH L
Sbjct: 339 KLVQEALETAMHGRTVILIAHRLSTVVNADVIAVVENGQVVETGTHHTL 485
Score = 26.2 bits (56), Expect(2) = 1e-11
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 21 AKTENSDSKGNAEKTSNSSPR*GNKCIGL*VRKA 54
++TEN +SK ++EK+S S *G C VRKA
Sbjct: 242 SETENCNSKSHSEKSSYPSS**GY*CP*YGVRKA 343
>BG662257
Length = 336
Score = 44.3 bits (103), Expect(2) = 1e-08
Identities = 20/30 (66%), Positives = 25/30 (82%)
Frame = +1
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKAD 82
K VQ+ALDR + RTT+V+AHRLSTI+ AD
Sbjct: 247 KVVQDALDRVRVDRTTIVVAHRLSTIKGAD 336
Score = 29.3 bits (64), Expect(2) = 1e-08
Identities = 10/35 (28%), Positives = 23/35 (65%)
Frame = +3
Query: 12 GCKSATLWKAKTENSDSKGNAEKTSNSSPR*GNKC 46
G + +T+W+A+ + K +++++ N + R* +KC
Sbjct: 123 G*RDSTIWRAEAAGGNCKSHSKESKNITTR*SHKC 227
>TC8591 similar to UP|O80680 (O80680) At2g41040 protein, partial (21%)
Length = 612
Score = 26.6 bits (57), Expect = 1.8
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = -3
Query: 13 CKSATLWKAKTENSDSKGNAEKTSNSSPR 41
CK+A W A TE D KG+ E ++ +S R
Sbjct: 151 CKAAPAWTASTE-PDEKGSLETSALTSAR 68
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.335 0.142 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,918,841
Number of Sequences: 28460
Number of extensions: 17315
Number of successful extensions: 115
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of query: 142
length of database: 4,897,600
effective HSP length: 81
effective length of query: 61
effective length of database: 2,592,340
effective search space: 158132740
effective search space used: 158132740
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0346.4