BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0343.1
(338 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780009 168 1e-42
BP075943 98 2e-21
BP043850 88 2e-18
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 83 6e-17
BP084332 79 8e-16
BP057233 76 9e-15
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 72 1e-13
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 72 1e-13
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 68 2e-12
AV424544 61 3e-10
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 40 2e-07
AV766665 49 1e-06
BP084259 48 2e-06
BP066094 48 3e-06
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 47 5e-06
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 44 5e-05
TC9992 42 1e-04
BP042669 35 0.002
AV411594 38 0.003
TC18909 36 0.011
>AV780009
Length = 529
Score = 168 bits (425), Expect = 1e-42
Identities = 82/169 (48%), Positives = 109/169 (63%)
Frame = -1
Query: 152 ALKRVLRYVRGTLTYGLHLYPSPIAKLVSYTDADWGGCPDTRRSTSGYCVFLGDNLISWS 211
A RVLRYV+G GL KL +Y+D+DW GCPDTRRS +GY +FLG +LISW
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 212 SKRQPTLSRSSAEAEYRGVANVVSESCWIRNLLLELHFPLSQATLVHCDNVSAIYLSGNP 271
+K+Q T+SRSS+EAEYR +A V E W+ L L + + CDN SA++++ NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 272 VHHQRTKHIEMDIHFVREKVARGQARVLHVPSRHQIADIFTKGLPRVLF 320
H+RTKHIE+D H VR K+ G +L + + HQ+ADIFTK P + F
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKPCPHLCF 20
>BP075943
Length = 547
Score = 97.8 bits (242), Expect = 2e-21
Identities = 59/164 (35%), Positives = 88/164 (52%), Gaps = 1/164 (0%)
Frame = -3
Query: 16 ILLYVDDIILVASSHALRKSFMALLASEFAMKDLGPLSYFLGIAVTRHAGGLFLSQSTYA 75
+LLYVDD++L + + + L +F +KDLG YFLG+ + R G+ L+Q YA
Sbjct: 521 LLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYA 342
Query: 76 SEIIARAGMASCNPSATPVDT-KQKLSSSSGTPCEDATLYRSLAGALQYLTFTRPDISYA 134
++I+ +G P T Q L +++GTP D YR + G L YL TRPDI++A
Sbjct: 341 LQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFA 162
Query: 135 VQQVCLHMHAPHTEHMLALKRVLRYVRGTLTYGLHLYPSPIAKL 178
V Q+ + AP H L L+Y+ G+ GL YP+ + L
Sbjct: 161 VNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGL-FYPASSSTL 33
>BP043850
Length = 515
Score = 87.8 bits (216), Expect = 2e-18
Identities = 50/104 (48%), Positives = 62/104 (59%)
Frame = -1
Query: 235 SESCWIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHFVREKVARG 294
SE W+R LL EL F SQ T +H DN SAI ++ NPV+H+ T+HIE+D H VRE R
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 295 QARVLHVPSRHQIADIFTKGLPRVLFDDFRSSLSVGEPPASTAG 338
+ HV + QIADI TK L R + S L + E PAS G
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFLVSKLMLVESPASI*G 204
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 83.2 bits (204), Expect = 6e-17
Identities = 37/77 (48%), Positives = 52/77 (67%)
Frame = +1
Query: 239 WIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHFVREKVARGQARV 298
W+ LL +L P Q LV+CDN SA +++ NPV H+RTKHIE+D H VRE++ +G +
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 299 LHVPSRHQIADIFTKGL 315
L + S +ADI+TK L
Sbjct: 184 LPISSSEPLADIYTKAL 234
>BP084332
Length = 368
Score = 79.3 bits (194), Expect = 8e-16
Identities = 43/107 (40%), Positives = 63/107 (58%)
Frame = +2
Query: 215 QPTLSRSSAEAEYRGVANVVSESCWIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHH 274
Q T++ S+AEAEY A ++ W+++ L + L ++CDN +AI LS NP+ H
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILH 208
Query: 275 QRTKHIEMDIHFVREKVARGQARVLHVPSRHQIADIFTKGLPRVLFD 321
R KHIE+ HF+R+ V +G + V + HQ ADIFTK L F+
Sbjct: 209 SRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFN 349
>BP057233
Length = 473
Score = 75.9 bits (185), Expect = 9e-15
Identities = 35/84 (41%), Positives = 52/84 (61%)
Frame = -2
Query: 250 PLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHFVREKVARGQARVLHVPSRHQIAD 309
P+ + ++ CDN+SA L+ NPV H R+KHIE+D+H++R++V + + V +VP+ QIAD
Sbjct: 466 PMPRKPILWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIAD 287
Query: 310 IFTKGLPRVLFDDFRSSLSVGEPP 333
TK L F R L V P
Sbjct: 286 CLTKPLSHTRFSQLRDKLGVIHSP 215
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 72.4 bits (176), Expect = 1e-13
Identities = 44/120 (36%), Positives = 62/120 (51%), Gaps = 8/120 (6%)
Frame = +2
Query: 12 DIAYILLYVDDIILVASSHALRKSFMALLASEFAMKDLGPLSYFLGIAVTRHAGG--LFL 69
D +LLYVDD+++V + + A LA EF MKDLGP + LG+ + R ++L
Sbjct: 83 DFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDMKDLGPANKILGMQIHRDRKDRRIWL 262
Query: 70 SQSTYASEIIARAGMASCNPSATPVDTKQKLSSSSGTPCEDATL------YRSLAGALQY 123
SQ Y +++ R M CNP +TP+ KLSSS E + Y S G+L Y
Sbjct: 263 SQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSMIPSSEAERMEMSRVPYASAVGSLMY 442
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 72.4 bits (176), Expect = 1e-13
Identities = 34/78 (43%), Positives = 50/78 (63%)
Frame = +3
Query: 236 ESCWIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHFVREKVARGQ 295
E+ W+ L +L +++CDN SA++L+ N V H+RT++IE+D H V KV G
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 296 ARVLHVPSRHQIADIFTK 313
+LHVPS Q+AD+FTK
Sbjct: 201 LHLLHVPSSDQVADVFTK 254
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 68.2 bits (165), Expect = 2e-12
Identities = 37/100 (37%), Positives = 55/100 (55%)
Frame = +1
Query: 239 WIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHFVREKVARGQARV 298
W+ +L +L P +L++CD+ SA +++ N V H+RTKH+++D H VREK+ +
Sbjct: 13 WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKLFHL 192
Query: 299 LHVPSRHQIADIFTKGLPRVLFDDFRSSLSVGEPPASTAG 338
L + S Q ADI TK L F S L V +S G
Sbjct: 193 LPISSVDQTADILTKPLESGPFSHLVSKLGVLNIYSSACG 312
>AV424544
Length = 276
Score = 60.8 bits (146), Expect = 3e-10
Identities = 34/74 (45%), Positives = 42/74 (55%)
Frame = +3
Query: 1 DHSLFIFRRGTDIAYILLYVDDIILVASSHALRKSFMALLASEFAMKDLGPLSYFLGIAV 60
DHSLF R IL+YVDD+IL + + L +F +KDLG L YFLG+ V
Sbjct: 54 DHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQFRIKDLGTLKYFLGLEV 233
Query: 61 TRHAGGLFLSQSTY 74
R + GLFLSQ Y
Sbjct: 234 ARSSCGLFLSQRKY 275
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 39.7 bits (91), Expect(2) = 2e-07
Identities = 16/36 (44%), Positives = 24/36 (66%)
Frame = +2
Query: 251 LSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHF 286
L+ T+++CDN AIYL N H R+KHI++ H+
Sbjct: 155 LTPDTVLYCDNQGAIYLGQNSTFHSRSKHIDVRYHW 262
Score = 31.2 bits (69), Expect(2) = 2e-07
Identities = 13/42 (30%), Positives = 22/42 (51%)
Frame = +3
Query: 208 ISWSSKRQPTLSRSSAEAEYRGVANVVSESCWIRNLLLELHF 249
++W S+ Q ++ S+AEAE+ E W++N L F
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWF 152
>AV766665
Length = 601
Score = 48.9 bits (115), Expect = 1e-06
Identities = 27/74 (36%), Positives = 40/74 (53%)
Frame = +3
Query: 155 RVLRYVRGTLTYGLHLYPSPIAKLVSYTDADWGGCPDTRRSTSGYCVFLGDNLISWSSKR 214
R+ Y++ G + + +T AD+ G R ST GY +FL NL++W SK+
Sbjct: 372 RIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTWRSKQ 551
Query: 215 QPTLSRSSAEAEYR 228
Q ++RSS EAE R
Sbjct: 552 QNIIARSSGEAELR 593
>BP084259
Length = 385
Score = 48.1 bits (113), Expect = 2e-06
Identities = 20/59 (33%), Positives = 33/59 (55%)
Frame = -3
Query: 228 RGVANVVSESCWIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHF 286
R + + +E W++ LL EL F L + + +N++A Y+ N V H KH+ +D HF
Sbjct: 179 RTIGSTAAEFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDCHF 3
>BP066094
Length = 532
Score = 47.8 bits (112), Expect = 3e-06
Identities = 24/58 (41%), Positives = 36/58 (61%)
Frame = +2
Query: 1 DHSLFIFRRGTDIAYILLYVDDIILVASSHALRKSFMALLASEFAMKDLGPLSYFLGI 58
D +LF DI + +YVDDII +++ +L K F ++ +EF M+ +G L YFLGI
Sbjct: 356 DTTLFCKTYKDDILIVQIYVDDIIFGSANPSLCKEFSEMMQAEFEMRMMGELKYFLGI 529
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 47.0 bits (110), Expect = 5e-06
Identities = 42/144 (29%), Positives = 64/144 (44%), Gaps = 2/144 (1%)
Frame = -1
Query: 71 QSTYASEIIARAGMASCNPSATPVDTKQ-KLSSSSGTPCEDATLYRSLAGALQYLTFTRP 129
Q Y +I+ + M + +T + K+ + +G D+T Y+SL G+++YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 130 DISYAVQQVCLHMHAPHTEHMLALKRVLRYVRGTLTYGLHLYPSPIAKLVSYTDADWGGC 189
DI V M P H +R LRY++GTL G+ + + +
Sbjct: 559 DIVCGVGLRSRFME-P*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSL---------- 413
Query: 190 PDTRRST-SGYCVFLGDNLISWSS 212
DT+ T GY LG ISWSS
Sbjct: 412 -DTQIVTRPGYAFHLGRGAISWSS 344
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein,
partial (4%)
Length = 528
Score = 43.5 bits (101), Expect = 5e-05
Identities = 22/59 (37%), Positives = 35/59 (59%)
Frame = +3
Query: 271 PVHHQRTKHIEMDIHFVREKVARGQARVLHVPSRHQIADIFTKGLPRVLFDDFRSSLSV 329
P+ T +IE HF+R++V +G+ + H + Q+ADI TKGL F + R+ L+V
Sbjct: 45 PMDVVNTLNIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 32.3 bits (72), Expect = 0.12
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +2
Query: 260 DNVSAIYLSGNPVHHQRTKHIE 281
DN SAI L+ NPV H R+KHIE
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>TC9992
Length = 508
Score = 42.4 bits (98), Expect = 1e-04
Identities = 32/104 (30%), Positives = 51/104 (48%), Gaps = 4/104 (3%)
Frame = +3
Query: 231 ANVVSESCWIRNLLLELHFPLSQATLVHCDNVSAIYLSGNPVHHQRTKHIEMDIHFVRE- 289
A VV+ WI+ LLL+L + DN + I +S +P+ ++T ++ +F+RE
Sbjct: 60 ATVVNPPLWIQKLLLDLPMEPMEHPKFFVDN*AIISISAHPLFQRKTHPSKIQFYFLREA 239
Query: 290 ---KVARGQARVLHVPSRHQIADIFTKGLPRVLFDDFRSSLSVG 330
+ R R+LH R Q ++F + LP F F SL G
Sbjct: 240 EW*EFRRSNLRILH-S*RAQWHEVFIQALPPSEF*VFS*SLPGG 368
>BP042669
Length = 435
Score = 35.4 bits (80), Expect(2) = 0.002
Identities = 16/23 (69%), Positives = 18/23 (77%)
Frame = -3
Query: 310 IFTKGLPRVLFDDFRSSLSVGEP 332
IFT GLPRV+F DFRSS S+ P
Sbjct: 259 IFTTGLPRVIFGDFRSSASMNLP 191
Score = 21.9 bits (45), Expect(2) = 0.002
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -2
Query: 329 VGEPPASTAG 338
+ EPPASTAG
Sbjct: 206 INEPPASTAG 177
>AV411594
Length = 244
Score = 37.7 bits (86), Expect = 0.003
Identities = 20/65 (30%), Positives = 30/65 (45%)
Frame = +2
Query: 55 FLGIAVTRHAGGLFLSQSTYASEIIARAGMASCNPSATPVDTKQKLSSSSGTPCEDATLY 114
FL + + G+ LS+ YA +++ C P P+D KL+S+ G D TLY
Sbjct: 50 FLRT*IAKSKKGILLSRRKYALQLLDDTRNMGCKPPPFPLDPSIKLNSTDGELLSDVTLY 229
Query: 115 RSLAG 119
G
Sbjct: 230 IRFIG 244
>TC18909
Length = 621
Score = 35.8 bits (81), Expect = 0.011
Identities = 26/77 (33%), Positives = 36/77 (45%), Gaps = 1/77 (1%)
Frame = +2
Query: 127 TRPDISYAVQQVCLHMHAPHTEHMLALKRVLRYVRGTL-TYGLHLYPSPIAKLVSYTDAD 185
T P IS++V +VC + EH A K +L L T G + + +++DAD
Sbjct: 326 TSPQISFSVNKVC*FLSESLEEHGTAGKCILNVT*KAL*TMGFFIQTN----FPAFSDAD 493
Query: 186 WGGCPDTRRSTSGYCVF 202
W D RRST VF
Sbjct: 494 WASNVDDRRSTFWEIVF 544
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,357,265
Number of Sequences: 28460
Number of extensions: 95237
Number of successful extensions: 508
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 503
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 503
length of query: 338
length of database: 4,897,600
effective HSP length: 91
effective length of query: 247
effective length of database: 2,307,740
effective search space: 570011780
effective search space used: 570011780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0343.1