BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0342b.11
(137 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10692 weakly similar to UP|BAD08916 (BAD08916) Calcium-binding... 125 3e-30
TC19367 homologue to UP|Q8QFN0 (Q8QFN0) Guanylate cyclase activa... 82 4e-17
TC10026 33 0.023
CB828334 30 0.19
TC10825 similar to GB|AAP68339.1|31711966|BT008900 At1g74740 {Ar... 29 0.33
BP063254 27 1.2
TC11868 similar to UP|Q8S9A5 (Q8S9A5) Glucosyltransferase like p... 27 1.6
CB828195 25 3.6
AI967538 25 6.2
TC20119 25 6.2
TC8769 similar to UP|Q42438 (Q42438) Calcium-dependent protein k... 24 8.1
TC13249 similar to PIR|T05606|T05606 protein kinase homolog F9D1... 24 8.1
>TC10692 weakly similar to UP|BAD08916 (BAD08916) Calcium-binding EF-hand
family protein-like, partial (32%)
Length = 634
Score = 125 bits (314), Expect = 3e-30
Identities = 63/139 (45%), Positives = 97/139 (69%), Gaps = 2/139 (1%)
Frame = +2
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
M V I+DG T+ +F+ DE +F SV +FA LD N DG+LS E+ + +R++E HFG
Sbjct: 29 MGVVIIDGTTVRDFVNDEASFTKSVDEQFAALDLNNDGVLSRAELRTAFESMRLIEAHFG 208
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV P++L ++Y S+F FD + +G+VD EF+ E +++MLA+ADG+G P+QMALE+
Sbjct: 209 IDVATPPEQLTKIYDSVFDTFDGDGSGTVDRLEFRSEIKKIMLAVADGLGTNPIQMALED 388
Query: 121 DS--ILKRAVERECNKVVA 137
DS +L++A + E +K+ A
Sbjct: 389 DSNNLLQKAADLEASKIGA 445
>TC19367 homologue to UP|Q8QFN0 (Q8QFN0) Guanylate cyclase activator protein
2 (Guanylate cyclase activating protein 2), partial (7%)
Length = 526
Score = 81.6 bits (200), Expect = 4e-17
Identities = 47/127 (37%), Positives = 76/127 (59%)
Frame = +1
Query: 5 ILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVK 64
+L+ +T+ F++D+ F+ V RFA++D N DG LS +E+ L G + +
Sbjct: 34 VLNSSTVTEFMDDKATFDSFVNERFAMVDENGDGELSRDEVRGGFGLFMPL----GSESQ 201
Query: 65 PDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEEDSIL 124
P E+ + +F +FD + NGS+DL+EFK E+M A+A G+G P+ +ALE DS+L
Sbjct: 202 PK-QEVDDMLDLIFKRFDEDQNGSLDLKEFKSLMTEIMNALARGIGGFPIMVALENDSLL 378
Query: 125 KRAVERE 131
+AV+ E
Sbjct: 379 MKAVQHE 399
>TC10026
Length = 599
Score = 32.7 bits (73), Expect = 0.023
Identities = 19/45 (42%), Positives = 24/45 (53%)
Frame = +2
Query: 5 ILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKEL 49
ILDG+TI LEDE+ F + N F LD G +S E+ L
Sbjct: 326 ILDGSTISLLLEDEDDFAMLAENLFTDLDVKDKGKISKSEIRNAL 460
>CB828334
Length = 541
Score = 29.6 bits (65), Expect = 0.19
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +2
Query: 65 PDPDELGRVYKSLFIQFDHNLNGSVDLEEFK 95
P DE K++F QFD + NG++D EE K
Sbjct: 26 PKIDESFGKCKAIFKQFDEDSNGAIDQEELK 118
>TC10825 similar to GB|AAP68339.1|31711966|BT008900 At1g74740 {Arabidopsis
thaliana;}, partial (32%)
Length = 1091
Score = 28.9 bits (63), Expect = 0.33
Identities = 11/17 (64%), Positives = 15/17 (87%)
Frame = +1
Query: 29 FALLDTNKDGLLSYEEM 45
F L+DT+KDG +SYEE+
Sbjct: 31 FTLMDTDKDGRVSYEEL 81
Score = 24.6 bits (52), Expect = 6.2
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +1
Query: 32 LDTNKDGLLSYEEML 46
+DT+KDG +SYEE +
Sbjct: 364 VDTDKDGRISYEEFV 408
>BP063254
Length = 474
Score = 26.9 bits (58), Expect = 1.2
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 33 DTNKDGLLSYEEMLKELQCLRVLETH 58
DT+K L Y+E +LQCLR+ + +
Sbjct: 131 DTSKYXLFKYQEYTLKLQCLRMCQXN 208
>TC11868 similar to UP|Q8S9A5 (Q8S9A5) Glucosyltransferase like protein
(Fragment), partial (25%)
Length = 485
Score = 26.6 bits (57), Expect = 1.6
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = +3
Query: 60 GIDVKPDPDELGRV 73
G D PDPDELGRV
Sbjct: 159 GADSVPDPDELGRV 200
>CB828195
Length = 551
Score = 25.4 bits (54), Expect = 3.6
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = +3
Query: 16 EDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFGID 62
ED+E V F + D N+DG + E+ + L C+ L+ GI+
Sbjct: 381 EDQEPSLEEVKQAFDVFDENRDGFIDARELHRVL-CVLGLKEEAGIE 518
>AI967538
Length = 432
Score = 24.6 bits (52), Expect = 6.2
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +2
Query: 64 KPDPDELGRVYKSLFIQFDHNLNGSVDLEEFK 95
+P+ DE+ K +F +FD N +G V +E+K
Sbjct: 134 QPNIDEM----KQVFDKFDANKDGKVSQQEYK 217
>TC20119
Length = 388
Score = 24.6 bits (52), Expect = 6.2
Identities = 14/29 (48%), Positives = 17/29 (58%)
Frame = +2
Query: 34 TNKDGLLSYEEMLKELQCLRVLETHFGID 62
T G S ++M K + CLRVLE GID
Sbjct: 44 TEAVGQSSEQDMEKIVTCLRVLEGLEGID 130
>TC8769 similar to UP|Q42438 (Q42438) Calcium-dependent protein kinase ,
partial (21%)
Length = 1144
Score = 24.3 bits (51), Expect = 8.1
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +2
Query: 32 LDTNKDGLLSYEE 44
+DT+KDG +SYEE
Sbjct: 197 VDTDKDGKISYEE 235
>TC13249 similar to PIR|T05606|T05606 protein kinase homolog F9D16.210 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(10%)
Length = 564
Score = 24.3 bits (51), Expect = 8.1
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = -2
Query: 9 ATIVNFLEDEEAFNISVCNRFALLDT 34
A + FL+DE+ F+IS+C ++ T
Sbjct: 278 ALLAWFLDDED*FSISICFTLCIIFT 201
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.139 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,809,692
Number of Sequences: 28460
Number of extensions: 18936
Number of successful extensions: 87
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 86
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 87
length of query: 137
length of database: 4,897,600
effective HSP length: 81
effective length of query: 56
effective length of database: 2,592,340
effective search space: 145171040
effective search space used: 145171040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0342b.11