BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.2
(88 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP074276 145 2e-36
TC16592 similar to UP|Q8L8H3 (Q8L8H3) Iron-stress related protei... 145 2e-36
TC8470 homologue to UP|Q8LK24 (Q8LK24) SOS2-like protein kinase,... 32 0.020
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 29 0.13
BP034485 29 0.13
TC8515 29 0.17
TC15676 similar to GB|AAG24281.1|10880503|AF195894 arabinogalact... 28 0.22
TC14944 similar to UP|LEC_LOTTE (P19664) Anti-H(O) lectin (LTA),... 28 0.28
TC17693 similar to UP|SFR2_HUMAN (Q01130) Splicing factor, argin... 28 0.37
AV421328 27 0.48
BP043042 27 0.63
TC17822 weakly similar to UP|Q8RZI5 (Q8RZI5) OJ1485_B09.11 prote... 27 0.63
AW164128 27 0.63
TC16408 similar to GB|AAM47476.1|21360559|AY113173 AT5g04990/MUG... 27 0.83
TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber... 26 1.1
TC16973 weakly similar to UP|Q96477 (Q96477) LRR protein, partia... 26 1.1
TC9637 homologue to UP|Q8YBN9 (Q8YBN9) Oligopeptide transport sy... 26 1.1
TC18140 similar to UP|KADD_ARATH (Q9FIJ7) Probable adenylate kin... 23 1.4
TC19470 26 1.4
BP043338 26 1.4
>BP074276
Length = 481
Score = 145 bits (365), Expect = 2e-36
Identities = 70/71 (98%), Positives = 71/71 (99%)
Frame = -2
Query: 18 QVASSRNVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSCSCSAASQLLNDRWTAAIQ 77
QVASSRNVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSCSCSAASQLLNDRWTAAIQ
Sbjct: 480 QVASSRNVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSCSCSAASQLLNDRWTAAIQ 301
Query: 78 SYNNSKDSSEK 88
SYNNS+DSSEK
Sbjct: 300 SYNNSQDSSEK 268
>TC16592 similar to UP|Q8L8H3 (Q8L8H3) Iron-stress related protein, partial
(15%)
Length = 757
Score = 145 bits (365), Expect = 2e-36
Identities = 77/88 (87%), Positives = 78/88 (88%)
Frame = +2
Query: 1 SDRLNRTNSIGNDLEKNQVASSRNVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSCS 60
SDRL+RTNSIGNDLEKNQ ASSRNVQVQD IDAVSS LVNEN YSRFVALDG CS
Sbjct: 218 SDRLDRTNSIGNDLEKNQFASSRNVQVQDRIDAVSSNLVNENPYSRFVALDG------CS 379
Query: 61 CSAASQLLNDRWTAAIQSYNNSKDSSEK 88
CSAASQLLNDRWTAAIQSYNNSKDSSEK
Sbjct: 380 CSAASQLLNDRWTAAIQSYNNSKDSSEK 463
>TC8470 homologue to UP|Q8LK24 (Q8LK24) SOS2-like protein kinase, partial
(29%)
Length = 807
Score = 32.0 bits (71), Expect = 0.020
Identities = 10/13 (76%), Positives = 13/13 (99%)
Frame = -2
Query: 50 LDGCSCSCSCSCS 62
++GCSC+CSCSCS
Sbjct: 50 VEGCSCTCSCSCS 12
Score = 23.9 bits (50), Expect = 5.4
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -2
Query: 55 CSCSCSCSAA 64
CSC+CSCS +
Sbjct: 41 CSCTCSCSCS 12
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 29.3 bits (64), Expect = 0.13
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = -3
Query: 47 FVALDGCSCSCSCSCSAAS 65
+V+ GC CSC C CS S
Sbjct: 63 WVSCGGCCCSCCCCCSCGS 7
>BP034485
Length = 485
Score = 29.3 bits (64), Expect = 0.13
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = -3
Query: 53 CSCSCSCSCSAASQLL 68
CSCSCSCSC + LL
Sbjct: 204 CSCSCSCSCLLSHLLL 157
Score = 23.9 bits (50), Expect = 5.4
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 53 CSCSCSCSCSAASQLL 68
CSCSCSC S LL
Sbjct: 198 CSCSCSCLLSHLLLLL 151
>TC8515
Length = 627
Score = 28.9 bits (63), Expect = 0.17
Identities = 14/40 (35%), Positives = 21/40 (52%), Gaps = 4/40 (10%)
Frame = +2
Query: 33 AVSSILVNEN----HYSRFVALDGCSCSCSCSCSAASQLL 68
A S + NEN +Y+ F +L CSCSC C + ++
Sbjct: 380 AASITVENENAWSIYYALFYSLIKMHCSCSCCCCSCMSII 499
>TC15676 similar to GB|AAG24281.1|10880503|AF195894 arabinogalactan protein
{Arabidopsis thaliana;} , partial (49%)
Length = 877
Score = 28.5 bits (62), Expect = 0.22
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = -3
Query: 7 TNSIGNDLEKNQVASSRNVQVQDHIDAVSSILVNENHYSRFV 48
TNSIGN KN VA +R + +Q + I H S+ +
Sbjct: 563 TNSIGNKPSKNNVALTRTLPIQQRVKLSKGI--KSKHKSQII 444
>TC14944 similar to UP|LEC_LOTTE (P19664) Anti-H(O) lectin (LTA), partial
(85%)
Length = 1155
Score = 28.1 bits (61), Expect = 0.28
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3
Query: 53 CSCSCSCSC 61
CSCSCSCSC
Sbjct: 940 CSCSCSCSC 914
Score = 24.6 bits (52), Expect = 3.1
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 55 CSCSCSCS 62
CSCSCSCS
Sbjct: 940 CSCSCSCS 917
>TC17693 similar to UP|SFR2_HUMAN (Q01130) Splicing factor,
arginine/serine-rich 2 (Splicing factor SC35) (SC-35)
(Splicing component, 35 kDa) (PR264 protein), partial
(8%)
Length = 751
Score = 27.7 bits (60), Expect = 0.37
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -2
Query: 45 SRFVALDGCSCSCSCSCS 62
S + L G CSCSCSCS
Sbjct: 288 SEEMMLGGSPCSCSCSCS 235
Score = 25.8 bits (55), Expect = 1.4
Identities = 11/26 (42%), Positives = 13/26 (49%)
Frame = -2
Query: 35 SSILVNENHYSRFVALDGCSCSCSCS 60
SS L+ + CSCSCSCS
Sbjct: 312 SSSLIEATSEEMMLGGSPCSCSCSCS 235
>AV421328
Length = 426
Score = 27.3 bits (59), Expect = 0.48
Identities = 10/13 (76%), Positives = 11/13 (83%)
Frame = -2
Query: 53 CSCSCSCSCSAAS 65
CSCSCSCS SA +
Sbjct: 44 CSCSCSCSYSAVN 6
Score = 23.5 bits (49), Expect = 7.0
Identities = 5/9 (55%), Positives = 9/9 (99%)
Frame = +2
Query: 53 CSCSCSCSC 61
C+C+C+C+C
Sbjct: 17 CNCNCNCNC 43
>BP043042
Length = 544
Score = 26.9 bits (58), Expect = 0.63
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +1
Query: 53 CSCSCSCSCSAAS 65
CSC CSCSC S
Sbjct: 421 CSCCCSCSCRITS 459
Score = 24.3 bits (51), Expect = 4.1
Identities = 7/15 (46%), Positives = 12/15 (79%)
Frame = +1
Query: 53 CSCSCSCSCSAASQL 67
C CSCSC +++S++
Sbjct: 427 CCCSCSCRITSSSEV 471
>TC17822 weakly similar to UP|Q8RZI5 (Q8RZI5) OJ1485_B09.11 protein,
partial (22%)
Length = 416
Score = 26.9 bits (58), Expect = 0.63
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = -1
Query: 53 CSCSCSCSCSA 63
CSCSCSCS SA
Sbjct: 35 CSCSCSCSYSA 3
Score = 23.5 bits (49), Expect = 7.0
Identities = 5/9 (55%), Positives = 9/9 (99%)
Frame = +2
Query: 53 CSCSCSCSC 61
C+C+C+C+C
Sbjct: 8 CNCNCNCNC 34
>AW164128
Length = 282
Score = 26.9 bits (58), Expect = 0.63
Identities = 8/9 (88%), Positives = 9/9 (99%)
Frame = +2
Query: 53 CSCSCSCSC 61
CSCSC+CSC
Sbjct: 206 CSCSCACSC 232
Score = 25.0 bits (53), Expect = 2.4
Identities = 8/9 (88%), Positives = 9/9 (99%)
Frame = +2
Query: 54 SCSCSCSCS 62
SCSCSC+CS
Sbjct: 203 SCSCSCACS 229
Score = 23.9 bits (50), Expect = 5.4
Identities = 7/9 (77%), Positives = 8/9 (88%)
Frame = +2
Query: 53 CSCSCSCSC 61
CSC+CSC C
Sbjct: 212 CSCACSC*C 238
>TC16408 similar to GB|AAM47476.1|21360559|AY113173 AT5g04990/MUG13_15
{Arabidopsis thaliana;}, partial (12%)
Length = 543
Score = 26.6 bits (57), Expect = 0.83
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = +2
Query: 42 NHYSRFVALDGCSCSCSCS 60
+H S FV L+ CSC+CS
Sbjct: 206 SHNSSFVCLEFYQCSCNCS 262
>TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber hypocotyls
(Arabinogalactan protein), partial (26%)
Length = 795
Score = 26.2 bits (56), Expect = 1.1
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +1
Query: 45 SRFVALDGCSCSCSCSCSAASQLLNDRWTAAIQSYNNSKDSS 86
S A GCS S +C +SQL + + + SY+ S+ S+
Sbjct: 277 SDLTAAGGCSGCLSTTCCCSSQLTSSQISTCTCSYSCSRCSA 402
>TC16973 weakly similar to UP|Q96477 (Q96477) LRR protein, partial (30%)
Length = 555
Score = 26.2 bits (56), Expect = 1.1
Identities = 14/52 (26%), Positives = 23/52 (43%)
Frame = -2
Query: 22 SRNVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSCSCSAASQLLNDRWT 73
++ +Q+ + LVN N S L GC C +++S L D+ T
Sbjct: 533 NKRLQITRWFLGTETCLVNLNVISELETLQGCHSGCKQMLTSSSSYL*DKLT 378
>TC9637 homologue to UP|Q8YBN9 (Q8YBN9) Oligopeptide transport system
permease protein APPB, partial (5%)
Length = 701
Score = 26.2 bits (56), Expect = 1.1
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +3
Query: 54 SCSCSCSCS 62
SCSCSCSCS
Sbjct: 576 SCSCSCSCS 602
Score = 24.6 bits (52), Expect = 3.1
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3
Query: 53 CSCSCSCS 60
CSCSCSCS
Sbjct: 579 CSCSCSCS 602
Score = 23.9 bits (50), Expect = 5.4
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +3
Query: 54 SCSCSCSCSAASQ 66
S SCSCSCS +++
Sbjct: 570 SSSCSCSCSCSTE 608
>TC18140 similar to UP|KADD_ARATH (Q9FIJ7) Probable adenylate kinase 2,
chloroplast precursor (ATP-AMP transphosphorylase) ,
partial (10%)
Length = 508
Score = 23.1 bits (48), Expect(2) = 1.4
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3
Query: 54 SCSCSCSCSAASQLLNDRWTA 74
SCSCSC C +++ R +A
Sbjct: 444 SCSCSCLCILFTEINKRRKSA 506
Score = 21.2 bits (43), Expect(2) = 1.4
Identities = 7/16 (43%), Positives = 9/16 (55%)
Frame = +3
Query: 44 YSRFVALDGCSCSCSC 59
+S + CSCSC C
Sbjct: 420 FSETSFVGSCSCSCLC 467
>TC19470
Length = 501
Score = 25.8 bits (55), Expect = 1.4
Identities = 7/13 (53%), Positives = 12/13 (91%)
Frame = +3
Query: 53 CSCSCSCSCSAAS 65
C+C+C+C+C+ AS
Sbjct: 126 CACACACACAWAS 164
Score = 25.4 bits (54), Expect = 1.8
Identities = 6/12 (50%), Positives = 11/12 (91%)
Frame = +3
Query: 53 CSCSCSCSCSAA 64
C+C+C+C+C+ A
Sbjct: 120 CACACACACACA 155
>BP043338
Length = 463
Score = 25.8 bits (55), Expect = 1.4
Identities = 12/19 (63%), Positives = 15/19 (78%), Gaps = 1/19 (5%)
Frame = +3
Query: 45 SRFV-ALDGCSCSCSCSCS 62
SRF+ +L +CSCSCSCS
Sbjct: 213 SRFICSLLRRACSCSCSCS 269
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.121 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,602,354
Number of Sequences: 28460
Number of extensions: 23635
Number of successful extensions: 471
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 339
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 435
length of query: 88
length of database: 4,897,600
effective HSP length: 64
effective length of query: 24
effective length of database: 3,076,160
effective search space: 73827840
effective search space used: 73827840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)
Lotus: description of TM0336.2