BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0335a.7
(632 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177509 58 4e-09
BP072317 48 4e-06
TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17... 47 1e-05
TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-li... 44 6e-05
BP036178 44 1e-04
BP049972 40 0.001
TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutat... 39 0.003
AV406521 39 0.003
BP083536 33 0.18
AV415611 32 0.31
TC19857 weakly similar to UP|RBS_LARLA (P16031) Ribulose bisphos... 30 1.2
TC9473 similar to AAS09986 (AAS09986) MYB transcription factor, ... 30 1.5
TC9034 30 1.5
BI418990 28 4.5
TC7960 homologue to UP|Q9SPB8 (Q9SPB8) Malate dehydrogenase , p... 28 5.8
TC19805 27 7.6
AV765466 27 9.9
TC10405 similar to UP|AAP51013 (AAP51013) 26K protein, partial (9%) 27 9.9
TC16122 27 9.9
>BF177509
Length = 472
Score = 58.2 bits (139), Expect = 4e-09
Identities = 36/140 (25%), Positives = 65/140 (45%)
Frame = +1
Query: 378 WLMEKPTSSWCRHAFSLYSRCDVVMNNLSEAFNAAIMLARDKPILTMMEWIRTYVMGRFP 437
W+ P W F R + N+ E+ N I+ A PI+ MME IR +M F
Sbjct: 19 WIRRIPPRLWATAYFE-GQRFGHLTANIVESLNTWILEASGLPIIQMMECIRRQLMTWFN 195
Query: 438 QMMEKLHKHHGQIMPRPLKRILHEVDHIKNWIPRVVGAQKYEVRHTITLERHVISLKDKS 497
+ E + ++P + + ++ + + +V+ A + E +++ ++++
Sbjct: 196 ERRETSMQWASILVPSAERCVAEALERARTY--QVLRANEAEFEVISHEGSNIVDIRNRC 369
Query: 498 CTCRFWELNGFPCRHAASAL 517
C CR W+L G PC HA +AL
Sbjct: 370 CLCRGWQLYGLPCAHAVAAL 429
>BP072317
Length = 436
Score = 48.1 bits (113), Expect = 4e-06
Identities = 21/44 (47%), Positives = 28/44 (62%)
Frame = +3
Query: 444 HKHHGQIMPRPLKRILHEVDHIKNWIPRVVGAQKYEVRHTITLE 487
H GQ+MP+PLKR+ EV N +P+ G YEV+H +TLE
Sbjct: 297 HVAQGQVMPKPLKRLAWEVKGTSNGLPQASGEGLYEVKHVVTLE 428
>TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;}, partial (6%)
Length = 527
Score = 46.6 bits (109), Expect = 1e-05
Identities = 28/64 (43%), Positives = 35/64 (53%), Gaps = 1/64 (1%)
Frame = +3
Query: 562 VCILPPMYKRSAGRPKKLRRRDPYEN-DPNPTKLQRGGATWKCSRCKQYGHNKRGCKNPV 620
V + PP KR GRPK P E+ D +LQ CS+CK+ GHNK+ CKN +
Sbjct: 30 VTVTPPPTKRPPGRPKM----KPVESIDMIKRQLQ-------CSKCKELGHNKKTCKNSL 176
Query: 621 PEEQ 624
P EQ
Sbjct: 177 PLEQ 188
>TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (12%)
Length = 588
Score = 44.3 bits (103), Expect = 6e-05
Identities = 30/100 (30%), Positives = 38/100 (38%), Gaps = 8/100 (8%)
Frame = +3
Query: 529 DDCYKREAYNATYENYVTPLPGPNQWI--------VTPHDPVCILPPMYKRSAGRPKKLR 580
+ C+ Y TY + P+P + W D V I PP R GRP+K R
Sbjct: 3 ESCFTVATYRKTYSQTIHPIPDKSLWKELSEGDENAAKADQVLINPPKSLRPPGRPRKKR 182
Query: 581 RRDPYENDPNPTKLQRGGATWKCSRCKQYGHNKRGCKNPV 620
R D K CSRC Q GH + C P+
Sbjct: 183 VR---AEDRGRVK-----RVVHCSRCNQTGHFRTTCAAPI 278
>BP036178
Length = 561
Score = 43.5 bits (101), Expect = 1e-04
Identities = 26/93 (27%), Positives = 39/93 (40%), Gaps = 9/93 (9%)
Frame = -2
Query: 537 YNATYENYVTPLPGPNQW---------IVTPHDPVCILPPMYKRSAGRPKKLRRRDPYEN 587
Y Y + P+P +QW + + I PP +R GRPKK R EN
Sbjct: 560 YRMAYSQIIIPIPDRSQWREHGEGAEGVGGARVDIVIRPPKTRRPPGRPKKKVLR--VEN 387
Query: 588 DPNPTKLQRGGATWKCSRCKQYGHNKRGCKNPV 620
P ++ + C RC GH+++ C P+
Sbjct: 386 FKRPKRIVQ------CGRCHMLGHSQKKCTMPI 306
>BP049972
Length = 496
Score = 39.7 bits (91), Expect = 0.001
Identities = 29/112 (25%), Positives = 40/112 (34%), Gaps = 17/112 (15%)
Frame = -1
Query: 526 QFVDDCYKREAYNATYENYVTPLPGPNQWI-----------------VTPHDPVCILPPM 568
+F + C+ Y TY + P+P + W + + I PP
Sbjct: 493 RFTESCFTVATYRKTYSQTIHPIPDRSFWNELSIEDANANKAVEDANASQSIEIVINPPK 314
Query: 569 YKRSAGRPKKLRRRDPYENDPNPTKLQRGGATWKCSRCKQYGHNKRGCKNPV 620
R GRP+K R R D K CSRC Q GH + C P+
Sbjct: 313 SLRPPGRPRKKRVR---AEDRGRVK-----RVVHCSRCNQTGHFRTTCAAPI 182
>TC18180 weakly similar to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (4%)
Length = 513
Score = 38.9 bits (89), Expect = 0.003
Identities = 30/84 (35%), Positives = 36/84 (42%), Gaps = 1/84 (1%)
Frame = +3
Query: 535 EAYNATYENYVTPLPGPNQWIVTPHDPVC-ILPPMYKRSAGRPKKLRRRDPYENDPNPTK 593
E+Y TY V P+P PV + PP +R GRP R Y + N K
Sbjct: 39 ESYRLTYSERVNPIPIMAVPESKDSQPVVTVTPPFTRRPPGRPATKR----YVSQ-NIVK 203
Query: 594 LQRGGATWKCSRCKQYGHNKRGCK 617
+ CSRCK GHNK CK
Sbjct: 204 REL-----HCSRCKGLGHNKSTCK 260
>AV406521
Length = 267
Score = 38.5 bits (88), Expect = 0.003
Identities = 21/63 (33%), Positives = 31/63 (48%), Gaps = 2/63 (3%)
Frame = +1
Query: 268 LAFGVVESETKDSWMWFMELLMDDID--PERSRRWVFISDQQKGLMEVFKEDMLHGSEHR 325
LAFGVV+ E D+W WF L ++ E + F+SD Q+G+ + + H
Sbjct: 1 LAFGVVDVENDDNWTWFRSELHKALEASTESMPQMGFLSDGQRGITDALRTSSQIHLIHS 180
Query: 326 LCV 328
CV
Sbjct: 181 ACV 189
>BP083536
Length = 377
Score = 32.7 bits (73), Expect = 0.18
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +1
Query: 496 KSCTCRFWELNGFPCRHAASALTF 519
K+C C WEL PCRHA + + +
Sbjct: 301 KACCCNLWELVEIPCRHALAGVHY 372
>AV415611
Length = 219
Score = 32.0 bits (71), Expect = 0.31
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +3
Query: 490 VISLKDKSCTCRFWELNGFPCRHAASAL 517
V+ + C+C+ W+L G PC HA + +
Sbjct: 108 VVDIDRWECSCKTWQLTGVPCCHAIAVI 191
>TC19857 weakly similar to UP|RBS_LARLA (P16031) Ribulose bisphosphate
carboxylase small chain, chloroplast precursor (RuBisCO
small subunit) , partial (65%)
Length = 501
Score = 30.0 bits (66), Expect = 1.2
Identities = 16/56 (28%), Positives = 25/56 (44%), Gaps = 9/56 (16%)
Frame = -3
Query: 485 TLERHVISLKDKSCTCRFWE---------LNGFPCRHAASALTFNGNDPKQFVDDC 531
+L HV++L C+ WE ++G PC HA S T + F++ C
Sbjct: 268 SLFEHVVNLLCNGLICKGWEVG*CFKLPIIDGIPCLHAESFRTIGCSLAFPFIEPC 101
>TC9473 similar to AAS09986 (AAS09986) MYB transcription factor, partial
(50%)
Length = 887
Score = 29.6 bits (65), Expect = 1.5
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +3
Query: 589 PNPTKLQRGGATWKCSRCKQYGHNKRGCKN 618
P+ L+ T +CS C GHN R C N
Sbjct: 153 PSFDPLRNAAMTRRCSHCSHGGHNARTCPN 242
>TC9034
Length = 583
Score = 29.6 bits (65), Expect = 1.5
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = +1
Query: 567 PMYKRSAGRPKKLRRRDPYENDPNPTKLQRGGATWKCSRCKQYGHNKRGCKNPVPEEQSE 626
P KR G + R+R E P + R KC+ C+++GH+ C+ + Q +
Sbjct: 43 PTLKRLPGWERINRKRGVTEG-PTVSHEARRSHRVKCANCEEFGHDSWECQRDKSKRQEK 219
Query: 627 V 627
V
Sbjct: 220 V 222
>BI418990
Length = 454
Score = 28.1 bits (61), Expect = 4.5
Identities = 27/91 (29%), Positives = 35/91 (37%), Gaps = 19/91 (20%)
Frame = +2
Query: 547 PLPGPNQWIVTPHDP-VCILPPMYKRSAGR------PKKLRR--RDPYENDPNPTKLQ-- 595
PLP N++ PH P + PP GR P LR R+P +P LQ
Sbjct: 125 PLPTQNRFRSHPHPPPLRHQPPPTHPQVGRNSP*PLPS*LRPPPREPPRRNPRIRPLQGL 304
Query: 596 --------RGGATWKCSRCKQYGHNKRGCKN 618
RGG W+ + G +R C N
Sbjct: 305 GSRALH*CRGGRCWQSAYAASSGRCRRDCWN 397
>TC7960 homologue to UP|Q9SPB8 (Q9SPB8) Malate dehydrogenase , partial
(97%)
Length = 1501
Score = 27.7 bits (60), Expect = 5.8
Identities = 17/52 (32%), Positives = 23/52 (43%), Gaps = 9/52 (17%)
Frame = +2
Query: 574 GRPKKLRRRD------PYENDPN---PTKLQRGGATWKCSRCKQYGHNKRGC 616
G + RRRD +E P P L+ TW+C RC+ + H GC
Sbjct: 209 GSRSRRRRRDRTTPFSSHEAQPPRFFPLPLRYRRHTWRCRRCQPHQHQI*GC 364
>TC19805
Length = 530
Score = 27.3 bits (59), Expect = 7.6
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +2
Query: 381 EKPTSS---WCRHAFSLYSRCDVVMNNLSEAFNAAIMLARDKPILTMMEW 427
E+PT++ +CR+ FS Y D V + +S +M++ + +L M W
Sbjct: 146 EQPTTTNREFCRYKFSWYLALDYVESLVSPY*ELCLMVSVEMRMLRQMPW 295
>AV765466
Length = 599
Score = 26.9 bits (58), Expect = 9.9
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -2
Query: 498 CTCRFWELNGFPCRHAASALT 518
C CR +E G CRH+ + L+
Sbjct: 592 CDCRLFEFRGILCRHSLAILS 530
>TC10405 similar to UP|AAP51013 (AAP51013) 26K protein, partial (9%)
Length = 590
Score = 26.9 bits (58), Expect = 9.9
Identities = 22/67 (32%), Positives = 34/67 (49%), Gaps = 5/67 (7%)
Frame = +3
Query: 183 TLLWRFSNELRKRAAGNTCKINFESPRSTLFPRFSRFYMCLDGCKRGFL----AGCRPFI 238
T +W+F++ +KR K +F + S FP FS F LD + GFL +G + +
Sbjct: 150 TTVWKFTSVQQKR*DVMIAKFSFLNFLSH-FPEFSDFGNGLDAKQMGFLTLIMSGIQTQL 326
Query: 239 GL-DGCH 244
+ GCH
Sbjct: 327 SV*KGCH 347
>TC16122
Length = 537
Score = 26.9 bits (58), Expect = 9.9
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 4/34 (11%)
Frame = +3
Query: 100 ETFRIKTLV----GEHNCARVFDNKSANVRWVKL 129
+T R+KT + GEHN R ++ ANV W+ L
Sbjct: 75 DTVRMKTAISLISGEHNALRRSVDEKANVFWLTL 176
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,957,906
Number of Sequences: 28460
Number of extensions: 206237
Number of successful extensions: 995
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 980
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 987
length of query: 632
length of database: 4,897,600
effective HSP length: 96
effective length of query: 536
effective length of database: 2,165,440
effective search space: 1160675840
effective search space used: 1160675840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0335a.7