BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0334a.8
(635 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11364 homologue to GB|CAA56354.1|510876|PVME1G NADP dependent ... 314 2e-86
TC17304 homologue to GB|CAA56354.1|510876|PVME1G NADP dependent ... 269 1e-72
CB827732 251 2e-67
AV407305 237 5e-63
TC8784 similar to UP|Q9M4Q9 (Q9M4Q9) NADP-dependent malic protei... 186 8e-48
AV770785 92 2e-19
AV777033 89 3e-18
BF177501 76 1e-14
AV411979 71 5e-13
TC8700 31 0.69
AV411798 28 4.5
AV777048 28 5.9
TC19789 28 5.9
TC10930 27 7.7
>TC11364 homologue to GB|CAA56354.1|510876|PVME1G NADP dependent malic
enzyme {Phaseolus vulgaris;} , partial (27%)
Length = 490
Score = 314 bits (805), Expect = 2e-86
Identities = 162/162 (100%), Positives = 162/162 (100%)
Frame = +1
Query: 337 LDRYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAL 396
LDRYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAL
Sbjct: 4 LDRYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAL 183
Query: 397 EISKQTKAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWAHEHEPVKALLDAVKAIKP 456
EISKQTKAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWAHEHEPVKALLDAVKAIKP
Sbjct: 184 EISKQTKAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWAHEHEPVKALLDAVKAIKP 363
Query: 457 TVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSEC 498
TVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSEC
Sbjct: 364 TVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSEC 489
>TC17304 homologue to GB|CAA56354.1|510876|PVME1G NADP dependent malic
enzyme {Phaseolus vulgaris;} , partial (23%)
Length = 606
Score = 269 bits (687), Expect = 1e-72
Identities = 133/134 (99%), Positives = 133/134 (99%)
Frame = +2
Query: 502 EAYTWSKGKAIFASGSPFDPVKYGGKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLL 561
EAYTWSKGKAIFASGSPFDPVKYGGKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLL
Sbjct: 2 EAYTWSKGKAIFASGSPFDPVKYGGKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLL 181
Query: 562 AASEALAAQVSQENYDKGLIYPPFTNIRKISAHIAASVAAKVYELGLASHLPRPKDLVKY 621
AASEALAAQVSQENYDKGLIYPPF NIRKISAHIAASVAAKVYELGLASHLPRPKDLVKY
Sbjct: 182 AASEALAAQVSQENYDKGLIYPPFPNIRKISAHIAASVAAKVYELGLASHLPRPKDLVKY 361
Query: 622 AESCMYSPGYRSYL 635
AESCMYSPGYRSYL
Sbjct: 362 AESCMYSPGYRSYL 403
>CB827732
Length = 438
Score = 251 bits (641), Expect = 2e-67
Identities = 123/145 (84%), Positives = 136/145 (92%)
Frame = +2
Query: 256 SLYTALGGVRPSSCLPVTIDVGTNNETLLNDEFYTGLRQKRATGKEYEELLDEFMRAVKQ 315
+LY+ALGGVRPS+CLPVTIDVGTNNE LLNDEFY GLRQKRATGKEY +LL EFM AVKQ
Sbjct: 2 ALYSALGGVRPSACLPVTIDVGTNNEQLLNDEFYIGLRQKRATGKEYYDLLHEFMTAVKQ 181
Query: 316 NYGEKVLIQFEDFANHNAFNLLDRYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLAD 375
NYGEKVL+QFEDFANHNAF LL +Y ++HLVFNDDIQGTASVVLAG++A+LKL+GGTL D
Sbjct: 182 NYGEKVLVQFEDFANHNAFELLAKYGTTHLVFNDDIQGTASVVLAGVVAALKLIGGTLGD 361
Query: 376 HTFLFLGAGEAGTGIAELIALEISK 400
HTFLFLGAGEAGTGIAELIALE+SK
Sbjct: 362 HTFLFLGAGEAGTGIAELIALEMSK 436
>AV407305
Length = 414
Score = 237 bits (604), Expect = 5e-63
Identities = 116/137 (84%), Positives = 126/137 (91%)
Frame = +1
Query: 314 KQNYGEKVLIQFEDFANHNAFNLLDRYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTL 373
KQ YGEKVLIQFEDFANHNAF+LL++Y S+HLVFNDDIQGTASVVLAGL+A+LKLVGG L
Sbjct: 4 KQTYGEKVLIQFEDFANHNAFDLLEKYRSTHLVFNDDIQGTASVVLAGLVAALKLVGGNL 183
Query: 374 ADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKVWLVDSKGLIVRSRLESLQHF 433
ADH FLFLGAGEAGTGIAELIALE SKQT +P+EE RK +WLVDSKGLIV SR ESLQHF
Sbjct: 184 ADHRFLFLGAGEAGTGIAELIALETSKQTNSPLEEVRKNIWLVDSKGLIVSSRKESLQHF 363
Query: 434 KKPWAHEHEPVKALLDA 450
KKPWAHEHEPVK L+DA
Sbjct: 364 KKPWAHEHEPVKNLVDA 414
>TC8784 similar to UP|Q9M4Q9 (Q9M4Q9) NADP-dependent malic protein (Malic
enzyme) , partial (18%)
Length = 729
Score = 186 bits (473), Expect = 8e-48
Identities = 89/113 (78%), Positives = 102/113 (89%)
Frame = +2
Query: 522 VKYGGKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLLAASEALAAQVSQENYDKGLI 581
V Y KV+ PGQANNAYIFPGFGLGLI+SG IRV D++LLAASEALA QV+QE++DKGLI
Sbjct: 2 VPYDDKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDLLLAASEALAEQVTQEHFDKGLI 181
Query: 582 YPPFTNIRKISAHIAASVAAKVYELGLASHLPRPKDLVKYAESCMYSPGYRSY 634
+PPFTNIRKISAHIAA VAAK YELGLA+ LP+PKDLVK+AESCMY+P YR+Y
Sbjct: 182 FPPFTNIRKISAHIAAKVAAKAYELGLATRLPQPKDLVKFAESCMYTPAYRTY 340
>AV770785
Length = 442
Score = 92.4 bits (228), Expect = 2e-19
Identities = 42/55 (76%), Positives = 49/55 (88%)
Frame = -1
Query: 579 GLIYPPFTNIRKISAHIAASVAAKVYELGLASHLPRPKDLVKYAESCMYSPGYRS 633
GLI+PPFT +RKISAHIAA VAAK YELGLA+ LP P+DLVK+AESCMY+P YR+
Sbjct: 442 GLIFPPFTTLRKISAHIAAQVAAKAYELGLATRLPPPQDLVKFAESCMYTPAYRT 278
>AV777033
Length = 592
Score = 88.6 bits (218), Expect = 3e-18
Identities = 48/109 (44%), Positives = 62/109 (56%), Gaps = 10/109 (9%)
Frame = +2
Query: 96 GNTLLRDPRYNKGLAFTEKERDAHYLRGLLPPAVFTQELQEKRTMHNIRQYE-------- 147
G +L DP +NK F ERD LRGLLPP V + E Q R M++ R E
Sbjct: 263 GADILHDPWFNKDTGFPLTERDRLGLRGLLPPRVISFEQQYDRFMNSYRSLEKNTQCQPE 442
Query: 148 --VPLHKYMAMMDLQERNERLFYKILIDNVEELLPIVYTPVVGEACQKY 194
V L K+ + L +RNE L+Y++LIDN++E PI+YTP VG CQ Y
Sbjct: 443 KVVSLAKWRILNRLHDRNETLYYRVLIDNIKEFAPIIYTPTVGLVCQNY 589
>BF177501
Length = 247
Score = 76.3 bits (186), Expect = 1e-14
Identities = 39/73 (53%), Positives = 52/73 (70%), Gaps = 1/73 (1%)
Frame = +1
Query: 512 IFASGSPFDPVKYG-GKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLLAASEALAAQ 570
+FASGSPF+ V G GKV QANN Y+FPG GLG ++SGA + D ML AASE LA+
Sbjct: 25 VFASGSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGARLITDGMLQAASECLASY 204
Query: 571 VSQENYDKGLIYP 583
+ +++ KG++YP
Sbjct: 205 MVEDDILKGILYP 243
>AV411979
Length = 408
Score = 71.2 bits (173), Expect = 5e-13
Identities = 45/137 (32%), Positives = 72/137 (51%), Gaps = 14/137 (10%)
Frame = +1
Query: 349 DDIQGTASVVLAGLLASLKLVGGTLAD---HTFLFLGAGEAGTGIAELIALEISK---QT 402
DD+QGTA V +AGLL +++ G + D + GAG AG G+ +++
Sbjct: 1 DDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNN 180
Query: 403 KAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWAHEHEPV--------KALLDAVKAI 454
+A E + + W+VD+KGLI R E++ P+A + + +L++ VK +
Sbjct: 181 EAAFESAKSQFWVVDAKGLITEGR-ENIDPDALPFARNLKEMDRQGLREGASLVEVVKQV 357
Query: 455 KPTVLIGSSGVGKTFTK 471
KP VL+G S VG F+K
Sbjct: 358 KPDVLLGLSAVGGLFSK 408
>TC8700
Length = 607
Score = 30.8 bits (68), Expect = 0.69
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 9/54 (16%)
Frame = +3
Query: 185 PVVGEACQKYGSIYK---------RPQGLYISLKEKGKILEVLKNWPEKTIQVI 229
P +G A K G +K +P I L +KG+ILEVL++ KT++ I
Sbjct: 87 PWLGNALPKVGFNFKQLHTSFAGWKPHATAIKLSDKGEILEVLEDCEGKTLKFI 248
>AV411798
Length = 397
Score = 28.1 bits (61), Expect = 4.5
Identities = 20/75 (26%), Positives = 33/75 (43%), Gaps = 8/75 (10%)
Frame = +3
Query: 178 LLPIVYTPVVGEACQKYGSIYKRPQGLYISLKEKGKILEVLKNWPEKTIQVI-------- 229
L PIV C G+ P G+ + L ++ I+ L P + IQ++
Sbjct: 168 LSPIVIFATNRGICNVRGTDMASPHGIPVDLLDRLAIVRTLIYGPAEMIQILAIRAQVEE 347
Query: 230 VVTDGERILGLGDLG 244
+V D E + LG++G
Sbjct: 348 LVVDEESLAFLGEIG 392
>AV777048
Length = 629
Score = 27.7 bits (60), Expect = 5.9
Identities = 25/91 (27%), Positives = 37/91 (40%), Gaps = 1/91 (1%)
Frame = +2
Query: 75 YGEDSATEDQLITPWTFSVASGNTLLRDPRYNKG-LAFTEKERDAHYLRGLLPPAVFTQE 133
Y T D + WT S + N LR+ R+ G + Y + LL + QE
Sbjct: 254 YSTKDVTLDPEGSTWTSS--NVNIELRNDRHQMGSFPVSSPCTPLDYNKELLKQTILNQE 427
Query: 134 LQEKRTMHNIRQYEVPLHKYMAMMDLQERNE 164
K +H + + HK +MD +RNE
Sbjct: 428 AIFKHQIHELHRV---YHKQRELMDEVKRNE 511
>TC19789
Length = 525
Score = 27.7 bits (60), Expect = 5.9
Identities = 24/93 (25%), Positives = 34/93 (35%), Gaps = 1/93 (1%)
Frame = +3
Query: 380 FLGAGEAG-TGIAELIALEISKQTKAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWA 438
FLG G T A+ ++ + ET + K LIV + S + + W
Sbjct: 138 FLGIASPGKTSFAQRLSEGNKSMSACDPTETFLETTATHKKHLIVMREIGSKETLRNLWK 317
Query: 439 HEHEPVKALLDAVKAIKPTVLIGSSGVGKTFTK 471
HE + + V A P SS TF K
Sbjct: 318 HEIQQTNVFVWFVDASNPDSWSASSEALLTFIK 416
>TC10930
Length = 577
Score = 27.3 bits (59), Expect = 7.7
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = +1
Query: 585 FTNIRKISAHIAASVAAKVYELGLASHLP 613
FT + ++AH K+ ELG++SH P
Sbjct: 208 FTRLSSVTAHALI*RLEKITELGISSHFP 294
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.137 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,834,446
Number of Sequences: 28460
Number of extensions: 125873
Number of successful extensions: 547
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 542
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 544
length of query: 635
length of database: 4,897,600
effective HSP length: 96
effective length of query: 539
effective length of database: 2,165,440
effective search space: 1167172160
effective search space used: 1167172160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0334a.8