BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0333b.4
(162 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11309 similar to UP|Q9FUV8 (Q9FUV8) Phloem serpin-1, partial (... 78 1e-24
AW428704 107 1e-24
TC16721 weakly similar to UP|P93692 (P93692) Serpin, partial (9%) 100 2e-22
TC15011 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragm... 91 7e-20
TC15717 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 87 1e-18
BP048545 81 1e-16
TC9744 similar to UP|Q40066 (Q40066) Protein zx, partial (7%) 74 9e-15
TC10025 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 74 2e-14
BP034134 65 7e-12
TC19574 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 64 1e-11
TC12056 weakly similar to UP|ILEU_HUMAN (P30740) Leukocyte elast... 60 2e-10
BP051691 49 4e-10
BP039383 57 2e-09
BP073359 54 2e-08
TC12352 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 41 1e-04
TC13700 similar to GB|AAM91354.1|22137018|AY133524 At2g28390/T1B... 25 5.0
BP068450 25 5.0
TC12204 weakly similar to UP|Q9ZSN3 (Q9ZSN3) NBS-LRR-like protei... 25 5.0
AV410527 25 6.5
>TC11309 similar to UP|Q9FUV8 (Q9FUV8) Phloem serpin-1, partial (10%)
Length = 472
Score = 77.8 bits (190), Expect(2) = 1e-24
Identities = 39/64 (60%), Positives = 47/64 (72%), Gaps = 2/64 (3%)
Frame = +3
Query: 90 FQKVFIKVHEEGTEAAAATIRALRGGGGPP--QGLKFVADHPFLFLIREDFSGTILFVGQ 147
F ++ +K+ EEGT AAAAT L GP G+ FVADHPF FLIREDF+GTILF+GQ
Sbjct: 90 FTRLSLKLDEEGTTAAAATAMLLASRSGPSVRAGIDFVADHPFFFLIREDFTGTILFIGQ 269
Query: 148 VLNP 151
VL+P
Sbjct: 270 VLHP 281
Score = 50.4 bits (119), Expect(2) = 1e-24
Identities = 24/36 (66%), Positives = 29/36 (79%), Gaps = 1/36 (2%)
Frame = +2
Query: 63 PFSQGDADFTKMVKVNTPFNE-LYVESIFQKVFIKV 97
PFS DADFTKMV+VN+P +E LY +S+F K FIKV
Sbjct: 5 PFSSTDADFTKMVEVNSPLDELLYADSVFHKAFIKV 112
>AW428704
Length = 369
Score = 107 bits (267), Expect = 1e-24
Identities = 63/101 (62%), Positives = 68/101 (66%)
Frame = +1
Query: 7 QDGLSPLIGKMASEPGFLEGRLPQHKVKLASFKIPRFDISFAFEASDVLKELGVVLPFSQ 66
+DGLS LI KMASEPGFLE ASDVLKE GVV PFSQ
Sbjct: 118 KDGLSDLIRKMASEPGFLE-------------------------ASDVLKEFGVVSPFSQ 222
Query: 67 GDADFTKMVKVNTPFNELYVESIFQKVFIKVHEEGTEAAAA 107
DADFTKMVKVN+P + L VESIFQKVFIKV+E+GTEAAAA
Sbjct: 223 RDADFTKMVKVNSPLDALSVESIFQKVFIKVNEQGTEAAAA 345
>TC16721 weakly similar to UP|P93692 (P93692) Serpin, partial (9%)
Length = 553
Score = 100 bits (248), Expect = 2e-22
Identities = 48/52 (92%), Positives = 50/52 (95%), Gaps = 1/52 (1%)
Frame = +2
Query: 112 LRGGGGPPQ-GLKFVADHPFLFLIREDFSGTILFVGQVLNPLGGANGTTTPV 162
LRGGGGPP GL+F+ADHPFLFLIREDFSGTILFVGQVLNPLGGANGTTTPV
Sbjct: 2 LRGGGGPPPPGLEFIADHPFLFLIREDFSGTILFVGQVLNPLGGANGTTTPV 157
>TC15011 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (20%)
Length = 623
Score = 91.3 bits (225), Expect = 7e-20
Identities = 52/92 (56%), Positives = 64/92 (69%), Gaps = 5/92 (5%)
Frame = +2
Query: 68 DADFTKMVKVNTPFNELYVESIFQKVFIKVHEEGTEAAAAT-----IRALRGGGGPPQGL 122
DA TKMV +T LYV +IF K FI+V+EEGTEAAAA+ +R+L+ +
Sbjct: 8 DAHLTKMVDSSTVGQGLYVSNIFHKSFIEVNEEGTEAAAASAVTIKLRSLQ----VLTRV 175
Query: 123 KFVADHPFLFLIREDFSGTILFVGQVLNPLGG 154
FVADHPFLF+IRED +GT+LF GQVLNPL G
Sbjct: 176 DFVADHPFLFVIREDLTGTVLFTGQVLNPLAG 271
>TC15717 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (26%)
Length = 925
Score = 87.0 bits (214), Expect = 1e-18
Identities = 44/53 (83%), Positives = 46/53 (86%)
Frame = +1
Query: 7 QDGLSPLIGKMASEPGFLEGRLPQHKVKLASFKIPRFDISFAFEASDVLKELG 59
+DGLS LI KMASEPGFLEG+LPQ KVKL F IPRFDISFAFEASDVLKE G
Sbjct: 766 KDGLSDLIRKMASEPGFLEGKLPQQKVKLNFFLIPRFDISFAFEASDVLKEFG 924
>BP048545
Length = 452
Score = 80.9 bits (198), Expect = 1e-16
Identities = 43/73 (58%), Positives = 53/73 (71%), Gaps = 2/73 (2%)
Frame = -2
Query: 82 NELYVESIFQKVFIKVHEEGTEAAAAT--IRALRGGGGPPQGLKFVADHPFLFLIREDFS 139
++LYV +IF K FI+V+EEGT AAAA+ I R P + FVADHPF F+IRE+ +
Sbjct: 439 SDLYVSNIFHKSFIEVNEEGTRAAAASAGIVLKRSLSPRPTRVDFVADHPFFFVIREEMT 260
Query: 140 GTILFVGQVLNPL 152
GTILF GQVLNPL
Sbjct: 259 GTILFTGQVLNPL 221
>TC9744 similar to UP|Q40066 (Q40066) Protein zx, partial (7%)
Length = 527
Score = 74.3 bits (181), Expect = 9e-15
Identities = 39/56 (69%), Positives = 44/56 (77%)
Frame = +1
Query: 100 EGTEAAAATIRALRGGGGPPQGLKFVADHPFLFLIREDFSGTILFVGQVLNPLGGA 155
+GTEA +AT+ + R G G FVADHPFLFLIREDF+GTILFVGQVLNPL GA
Sbjct: 1 KGTEATSATVVSGRKRLGGFLGTYFVADHPFLFLIREDFTGTILFVGQVLNPLEGA 168
>TC10025 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (15%)
Length = 672
Score = 73.6 bits (179), Expect = 2e-14
Identities = 42/68 (61%), Positives = 48/68 (69%), Gaps = 1/68 (1%)
Frame = +1
Query: 96 KVHEEGTEAAAATI-RALRGGGGPPQGLKFVADHPFLFLIREDFSGTILFVGQVLNPLGG 154
+V+EE T A AAT R + GG G + FVADHPFL L+RED +GTILFVGQVLNPL G
Sbjct: 1 EVNEEETVAVAATAARMVLGGAGLCPHIDFVADHPFLLLVREDITGTILFVGQVLNPLDG 180
Query: 155 ANGTTTPV 162
A TPV
Sbjct: 181 AG---TPV 195
>BP034134
Length = 556
Score = 64.7 bits (156), Expect = 7e-12
Identities = 29/37 (78%), Positives = 32/37 (86%)
Frame = -1
Query: 119 PQGLKFVADHPFLFLIREDFSGTILFVGQVLNPLGGA 155
P G+ F ADHPFLFLIRED +GTILF+GQVLNPL GA
Sbjct: 490 PTGIDFEADHPFLFLIREDLTGTILFIGQVLNPLDGA 380
>TC19574 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (8%)
Length = 414
Score = 63.9 bits (154), Expect = 1e-11
Identities = 31/52 (59%), Positives = 38/52 (72%)
Frame = -3
Query: 105 AAATIRALRGGGGPPQGLKFVADHPFLFLIREDFSGTILFVGQVLNPLGGAN 156
A A +L G P G+ FVA+HPFLFLIRE+ +GT LF+GQVLNPL GA+
Sbjct: 403 ATAAAGSLLGTYFRPAGIDFVANHPFLFLIREELTGTFLFIGQVLNPLDGAD 248
>TC12056 weakly similar to UP|ILEU_HUMAN (P30740) Leukocyte elastase
inhibitor (LEI) (Monocyte/neutrophil elastase inhibitor)
(M/NEI) (EI), partial (6%)
Length = 401
Score = 60.1 bits (144), Expect = 2e-10
Identities = 27/34 (79%), Positives = 30/34 (87%)
Frame = +2
Query: 124 FVADHPFLFLIREDFSGTILFVGQVLNPLGGANG 157
FVADHPFLFLIREDF+GTILF+GQVL+P G G
Sbjct: 5 FVADHPFLFLIREDFTGTILFIGQVLHPHGRGTG 106
>BP051691
Length = 428
Score = 48.9 bits (115), Expect(2) = 4e-10
Identities = 26/52 (50%), Positives = 38/52 (73%), Gaps = 3/52 (5%)
Frame = -2
Query: 65 SQGDADFTKMVKVNT---PFNELYVESIFQKVFIKVHEEGTEAAAATIRALR 113
S+ DA+FT MV + P +ELY++++F K FI+V+EEGTEAAAA+ L+
Sbjct: 427 SRCDANFTNMVDSPSDEFPSDELYIDNMFHKAFIEVNEEGTEAAAASSGRLK 272
Score = 30.0 bits (66), Expect(2) = 4e-10
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = -1
Query: 136 EDFSGTILFVGQVLNP 151
ED SGTILF+G+VL+P
Sbjct: 275 EDLSGTILFIGRVLHP 228
>BP039383
Length = 340
Score = 57.0 bits (136), Expect = 2e-09
Identities = 25/37 (67%), Positives = 30/37 (80%)
Frame = -1
Query: 118 PPQGLKFVADHPFLFLIREDFSGTILFVGQVLNPLGG 154
P + FVADHPFLF+IRE+ +GTILF GQV+NPL G
Sbjct: 316 PKTRVDFVADHPFLFVIREEMTGTILFTGQVVNPLAG 206
>BP073359
Length = 388
Score = 53.5 bits (127), Expect = 2e-08
Identities = 23/28 (82%), Positives = 27/28 (96%)
Frame = -3
Query: 124 FVADHPFLFLIREDFSGTILFVGQVLNP 151
FVADHPFLFLI+ED SGTILF+G+VL+P
Sbjct: 314 FVADHPFLFLIKEDLSGTILFIGRVLHP 231
>TC12352 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (25%)
Length = 799
Score = 40.8 bits (94), Expect = 1e-04
Identities = 18/29 (62%), Positives = 25/29 (86%)
Frame = +1
Query: 7 QDGLSPLIGKMASEPGFLEGRLPQHKVKL 35
QDGLS LI K++SEP FL+G+LP+ KV++
Sbjct: 712 QDGLSALIQKLSSEPCFLKGKLPRRKVRV 798
>TC13700 similar to GB|AAM91354.1|22137018|AY133524 At2g28390/T1B3.9
{Arabidopsis thaliana;}, partial (24%)
Length = 634
Score = 25.4 bits (54), Expect = 5.0
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = +2
Query: 77 VNTPFNELYVESIFQKVFIKVHEEG 101
+NTP + + +QK+F+ +H++G
Sbjct: 230 INTPQQQKRLYRAYQKLFVSMHDKG 304
>BP068450
Length = 387
Score = 25.4 bits (54), Expect = 5.0
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +3
Query: 109 IRALRGGGGPPQGLKFVADHPFLFLIR 135
+ ++RGG GPP A +P F +R
Sbjct: 216 VNSIRGGAGPPSEGSSSAAYPLAFSLR 296
>TC12204 weakly similar to UP|Q9ZSN3 (Q9ZSN3) NBS-LRR-like protein cD7
(Fragment), partial (3%)
Length = 328
Score = 25.4 bits (54), Expect = 5.0
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 113 RGGGGPPQGLKFVADHPFLFLIREDFSGTILFV 145
R G PP G+ + H F+F + D S ILF+
Sbjct: 272 RLSGKPPSGIDSSSGHSFMFKVMRDRS--ILFI 180
>AV410527
Length = 429
Score = 25.0 bits (53), Expect = 6.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1
Query: 112 LRGGGGPPQGLKFVADHPFLFL 133
+R GG +G+ +VAD PF L
Sbjct: 337 IRSGGHDYEGVSYVADVPFFIL 402
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.143 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,320,641
Number of Sequences: 28460
Number of extensions: 26186
Number of successful extensions: 144
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of query: 162
length of database: 4,897,600
effective HSP length: 83
effective length of query: 79
effective length of database: 2,535,420
effective search space: 200298180
effective search space used: 200298180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0333b.4