BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0331c.6
(426 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP062688 105 2e-23
TC19596 63 8e-11
BP052637 55 2e-10
NP459593 Pge1 protein [Lotus japonicus] 57 8e-09
AV407204 52 1e-07
BP038938 44 7e-05
BP069214 43 1e-04
AV778568 40 6e-04
BP056414 40 7e-04
TC19906 similar to PIR|AC1649|AC1649 phage related protein [impo... 37 0.008
BP053073 37 0.008
BP066140 35 0.024
TC7803 homologue to UP|Q9XQB1 (Q9XQB1) LHCII type III chlorophyl... 33 0.12
BP040790 33 0.12
TC11612 weakly similar to UP|Q8LAM5 (Q8LAM5) Protein disulfide i... 31 0.44
TC11157 30 0.99
TC16722 similar to UP|Q9FNY6 (Q9FNY6) Oxalyl-CoA decarboxylase, ... 30 0.99
TC14563 similar to UP|Q9SSP4 (Q9SSP4) F3N23.2 protein (At1g72820... 30 0.99
TC9288 29 1.3
TC11323 29 1.3
>BP062688
Length = 536
Score = 105 bits (261), Expect = 2e-23
Identities = 46/88 (52%), Positives = 64/88 (72%)
Frame = -1
Query: 31 LLALQKGLLHVWELGFRPVNCFVDCSEVQKVVITNSDVQNYWHGAVILLIREVIARNLSV 90
+++ KGLLH WELGF V C+VDC E+Q+V+ + DVQ YWH VI +IR V+AR+ +V
Sbjct: 398 IMSFHKGLLHTWELGFLHVVCYVDCLELQEVLTSTRDVQTYWHRDVIEMIRAVLARSWNV 219
Query: 91 SVTPVPKEQKMVVDALAKLAVREGWDWK 118
++ V ++ V DALA LA++EGWDWK
Sbjct: 218 TINHVTRDGNAVADALANLAIQEGWDWK 135
>TC19596
Length = 499
Score = 63.2 bits (152), Expect = 8e-11
Identities = 38/110 (34%), Positives = 59/110 (53%), Gaps = 1/110 (0%)
Frame = +1
Query: 1 MGGGGIRGDG-GRWVSGFAMAQGAGDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEVQ 59
MG GGI D G W+SGF GDA ++++ AL+ GL +W R C VDC ++
Sbjct: 46 MGMGGIVRDAHGAWISGFYAGSLGGDALRAEIAALKHGLTLLWNAHVRRATCEVDCLDIV 225
Query: 60 KVVITNSDVQNYWHGAVILLIREVIARNLSVSVTPVPKEQKMVVDALAKL 109
+ + N Q + + +L IR ++ R+ +V++ VP+E D LA L
Sbjct: 226 E-ALENDRYQFHALASELLDIRLLLDRDWTVTLAYVPREANAAADCLAGL 372
>BP052637
Length = 473
Score = 55.1 bits (131), Expect(2) = 2e-10
Identities = 27/45 (60%), Positives = 30/45 (66%)
Frame = -2
Query: 2 GGGGIRGDGGRWVSGFAMAQGAGDAFKSKLLALQKGLLHVWELGF 46
G G +R RWVSGFAMA G GDAF +KL AL + L HVW L F
Sbjct: 373 GSGVLRDKVXRWVSGFAMAFGTGDAFMAKLQALHESLQHVWGLRF 239
Score = 26.9 bits (58), Expect(2) = 2e-10
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -1
Query: 42 WELGFRPVNCFVDCSEVQKVVITNSDVQNY 71
+ L +R V CF C+E+ VV D+ ++
Sbjct: 119 YHLSYRKVRCFSSCAELVDVVNGEHDISHF 30
>NP459593 Pge1 protein [Lotus japonicus]
Length = 633
Score = 56.6 bits (135), Expect = 8e-09
Identities = 29/120 (24%), Positives = 59/120 (49%)
Frame = +1
Query: 6 IRGDGGRWVSGFAMAQGAGDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEVQKVVITN 65
+R G W+S + G AF +++LA++ G+ H ++G V C DCS+ + + +
Sbjct: 19 VRDSRGAWLSATLVCYNYGSAFLTEILAVKLGIRHALDIGHIYVFCLSDCSQALQALHAD 198
Query: 66 SDVQNYWHGAVILLIREVIARNLSVSVTPVPKEQKMVVDALAKLAVREGWDWKGQNKTEC 125
+++ +YW I + ++IA V++ + + + D L + A R ++ G C
Sbjct: 199 TNITSYWTREKICRVHDIIATLQDVNLQHIDRVKNNTADKLVREAGR--LEYMGSGSFRC 372
>AV407204
Length = 432
Score = 52.4 bits (124), Expect = 1e-07
Identities = 32/99 (32%), Positives = 50/99 (50%), Gaps = 2/99 (2%)
Frame = -2
Query: 12 RWVSGFAMA--QGAGDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEVQKVVITNSDVQ 69
+WV+GF + A +F ++ LAL+ GL W+ G R + C DC E+ + S
Sbjct: 431 KWVNGFISQYLEAASCSFLAEALALRDGLRLAWDNGTRKLVCNSDCKELIDAIADPSRAS 252
Query: 70 NYWHGAVILLIREVIARNLSVSVTPVPKEQKMVVDALAK 108
+ HG V+ I ++ RN V + ++ MV D LAK
Sbjct: 251 FHAHGWVLREIHALMTRNWRVELFWCCRDVNMVADCLAK 135
>BP038938
Length = 583
Score = 43.5 bits (101), Expect = 7e-05
Identities = 31/104 (29%), Positives = 50/104 (47%), Gaps = 2/104 (1%)
Frame = +3
Query: 135 PWLLYVHNNLAIIRSILDPTE--TYSRSIPELSGANLLTVQHGGWCVLEKGGKEKVRELL 192
PWL+Y + DP + TYS +PELSG+ + + GW +L + + +
Sbjct: 267 PWLMYFPK-YGDLYEFYDPVQRKTYSLQMPELSGSRVCYTK-DGWLLLY---RPRTHRVF 431
Query: 193 LWNPFLQEKIHLPSLKHENRIRNCILSCSPTETDQVCSIFLFPH 236
+NPF ++ I LP + E + SC+PT + C +F H
Sbjct: 432 FFNPFTRDIIKLP--RFEMTYQIVAFSCAPTSPN--CVLFTVKH 551
>BP069214
Length = 545
Score = 42.7 bits (99), Expect = 1e-04
Identities = 27/79 (34%), Positives = 44/79 (55%)
Frame = -2
Query: 30 KLLALQKGLLHVWELGFRPVNCFVDCSEVQKVVITNSDVQNYWHGAVILLIREVIARNLS 89
+LLA+ GL VW+ GFR V C D +E +V+ + + A+I I+++++R
Sbjct: 394 ELLAMWHGLDLVWDRGFRQVICQSDSTEALALVL-GVPPPRHRYAALIWSIKDLLSREWE 218
Query: 90 VSVTPVPKEQKMVVDALAK 108
VS+T + +E D LAK
Sbjct: 217 VSMTHMLREGNSCADFLAK 161
>AV778568
Length = 519
Score = 40.4 bits (93), Expect = 6e-04
Identities = 18/48 (37%), Positives = 34/48 (70%)
Frame = +1
Query: 52 FVDCSEVQKVVITNSDVQNYWHGAVILLIREVIARNLSVSVTPVPKEQ 99
+VD E Q+++++N +VQNY G I+ +R +IA++ +V+V V +E+
Sbjct: 373 YVDGQEWQQIIMSNREVQNYGQGNEIIEVRALIAKDGTVTVVHVSREE 516
>BP056414
Length = 606
Score = 40.0 bits (92), Expect = 7e-04
Identities = 28/112 (25%), Positives = 53/112 (47%), Gaps = 3/112 (2%)
Frame = -3
Query: 3 GGGIRGDGGRWVSGFAMAQGA---GDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEVQ 59
GG +R G W GFA + G AF +L+A+Q + + V D +V
Sbjct: 487 GGVVRDAAGSWSKGFARSLGVLRWHXAFFVELMAVQTAVDFIMSWDIPQVIIESDSQQVV 308
Query: 60 KVVITNSDVQNYWHGAVILLIREVIARNLSVSVTPVPKEQKMVVDALAKLAV 111
+++ + + +G + I++ I+ + S+ +T P+E + D LAK+ +
Sbjct: 307 ELLQNFNASSPHQYGEIAWDIQQKISVHGSIVITSTPREANFLADYLAKVGL 152
>TC19906 similar to PIR|AC1649|AC1649 phage related protein [imported] -
Listeria innocua (strain Clip11262)
{Listeria innocua;}, partial (4%)
Length = 509
Score = 36.6 bits (83), Expect = 0.008
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = -3
Query: 24 GDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEV 58
GD FK+++ AL+ GL+ +W++ R C VDC E+
Sbjct: 114 GDPFKAEVEALKLGLILMWDVNMRSAVCEVDCIEL 10
>BP053073
Length = 456
Score = 36.6 bits (83), Expect = 0.008
Identities = 30/125 (24%), Positives = 56/125 (44%), Gaps = 1/125 (0%)
Frame = -2
Query: 17 FAMAQGAGDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEVQKVVITNSDVQNYWHGAV 76
F++ G + K +LL + GL W G R + F D +V D + + + A+
Sbjct: 455 FSIFVGISEILKXELLXMLYGLQL*WSKGVRRMLVFTDSQLALSLVQLGCD-RFHQYAAI 279
Query: 77 ILLIREVIARNLSVSVTPVPKEQKMVVDALAKLAVREGWDWK-GQNKTECLLPPTTQSYP 135
+ +I +++ + V+ + +E VVD LAK R+ K ++ + LP Y
Sbjct: 278 VGMIHDLLRFDWQVNFRLILREGNAVVDLLAKDGTRDSCRLKLLEDPLQAALPLLADDYC 99
Query: 136 WLLYV 140
L++
Sbjct: 98 GTLFM 84
>BP066140
Length = 320
Score = 35.0 bits (79), Expect = 0.024
Identities = 22/76 (28%), Positives = 32/76 (41%)
Frame = -2
Query: 36 KGLLHVWELGFRPVNCFVDCSEVQKVVITNSDVQNYWHGAVILLIREVIARNLSVSVTPV 95
+GL WE G R + C DC +V K + A + R +I + P
Sbjct: 319 RGLSLAWERGIRKLLCISDCEDVVKALTGLGSFHLDSWAAYLAEARSLIHPEWECLLLPH 140
Query: 96 PKEQKMVVDALAKLAV 111
+E+ DALAKL +
Sbjct: 139 HRERNKPADALAKLGM 92
>TC7803 homologue to UP|Q9XQB1 (Q9XQB1) LHCII type III chlorophyll a/b
binding protein, complete
Length = 1178
Score = 32.7 bits (73), Expect = 0.12
Identities = 15/58 (25%), Positives = 31/58 (52%)
Frame = -2
Query: 140 VHNNLAIIRSILDPTETYSRSIPELSGANLLTVQHGGWCVLEKGGKEKVRELLLWNPF 197
+HN+L I++ + +E + +P +S + T H WC+ + ++ + E WNP+
Sbjct: 409 IHNHLGILQLSMKGSEL--KRVPSISPCLVHTTNHFPWCISQFP*RQHL*ECWSWNPW 242
>BP040790
Length = 344
Score = 32.7 bits (73), Expect = 0.12
Identities = 19/46 (41%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Frame = +2
Query: 14 VSGFAMAQGAGDAFKS-KLLALQKGLLHVWELGFRPVNCFVDCSEV 58
V G +A+G F + AL+ GL+ VW G V C VDC EV
Sbjct: 116 VVGLCIARGRRKHFHGWRQYALRNGLMSVWNWGLIYVACKVDCVEV 253
>TC11612 weakly similar to UP|Q8LAM5 (Q8LAM5) Protein disulfide
isomerase-like, partial (35%)
Length = 1014
Score = 30.8 bits (68), Expect = 0.44
Identities = 18/56 (32%), Positives = 31/56 (55%)
Frame = +1
Query: 193 LWNPFLQEKIHLPSLKHENRIRNCILSCSPTETDQVCSIFLFPHEAYGYGPNILFY 248
LW PFL EK+H SL + + + +L + T++D++ FL EA + ++ Y
Sbjct: 838 LW*PFLLEKVHQQSLVNPIQ-KQLLLFATSTDSDKILPFFL---EAAKFFSQLILY 993
>TC11157
Length = 519
Score = 29.6 bits (65), Expect = 0.99
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = -3
Query: 324 DPFPHTFPCLRQLLVCNHELFHIQISMEYNKIFGIAVHKLNFSHMIW 370
DPFPH P ++ C+H H + E + +A H+L H ++
Sbjct: 205 DPFPHLAPKVKSD*FCHH-FGHPRTR*ENHGFHNLAAHRLRIGHFLY 68
>TC16722 similar to UP|Q9FNY6 (Q9FNY6) Oxalyl-CoA decarboxylase, partial
(15%)
Length = 555
Score = 29.6 bits (65), Expect = 0.99
Identities = 17/53 (32%), Positives = 22/53 (41%), Gaps = 5/53 (9%)
Frame = -1
Query: 229 CSIFLFPHEAY---GYGPNILFYCEVGDKQWFAVDYR--KDLVRASPPEEDGV 276
CS+F Y GYGP+ F+ GD W Y+ K L PE +
Sbjct: 522 CSVFTSNSAVYIPYGYGPSFFFFLIYGDSNWV*SSYKLSKPLNTNPVPENSSI 364
>TC14563 similar to UP|Q9SSP4 (Q9SSP4) F3N23.2 protein (At1g72820/F3N23_2),
partial (55%)
Length = 1025
Score = 29.6 bits (65), Expect = 0.99
Identities = 15/38 (39%), Positives = 21/38 (54%), Gaps = 5/38 (13%)
Frame = -3
Query: 169 LLTVQHGGW-----CVLEKGGKEKVRELLLWNPFLQEK 201
+LTVQ G W C LE+ +++V LLWN L +
Sbjct: 252 ILTVQFGNWNHVLWCKLEREEEKRVCFFLLWNQVLDRR 139
>TC9288
Length = 553
Score = 29.3 bits (64), Expect = 1.3
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = +3
Query: 209 HENRIRNCILSCSPTETDQVCSIFLFPHEAYGYGPNILFYCEVGDK 254
HE +R ++S P E ++ +FLFP+ + C VGD+
Sbjct: 333 HETNVR--VISFCPQEGNRAIKLFLFPYVFFSIRSYYKILCIVGDR 464
>TC11323
Length = 657
Score = 29.3 bits (64), Expect = 1.3
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = +2
Query: 2 GGGGIRGDGG-RWVSGFAMAQGAGDAFKSKLLALQKGLLHVWELGFRPVNCFVDCSEV 58
GGGG+ DG ++ GF++ K L A+ +GLL LG+ + D + V
Sbjct: 77 GGGGLLRDGSSNFIFGFSIFLAVAQIMKLSLCAILEGLLVCKPLGYDGIGIECDSNIV 250
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.142 0.452
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,342,202
Number of Sequences: 28460
Number of extensions: 158245
Number of successful extensions: 924
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 915
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 922
length of query: 426
length of database: 4,897,600
effective HSP length: 93
effective length of query: 333
effective length of database: 2,250,820
effective search space: 749523060
effective search space used: 749523060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0331c.6