BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0330.6
(706 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV427022 298 2e-81
AV412895 237 5e-63
TC13572 145 2e-38
BP041113 152 1e-37
AV418280 119 2e-27
AV767121 115 2e-26
BP056597 95 4e-20
AV414139 92 3e-19
AV765930 86 2e-17
AU089120 62 1e-16
AU089037 78 4e-15
BP057427 77 1e-14
TC15601 75 3e-14
AV428268 75 3e-14
TC20217 73 1e-13
TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular so... 72 2e-13
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 72 3e-13
AV780221 72 4e-13
TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, part... 70 1e-12
BP033775 53 1e-12
>AV427022
Length = 429
Score = 298 bits (764), Expect = 2e-81
Identities = 143/143 (100%), Positives = 143/143 (100%)
Frame = +1
Query: 532 LN*QVLHNALEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLL 591
LN*QVLHNALEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLL
Sbjct: 1 LN*QVLHNALEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLL 180
Query: 592 QFSSIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSR 651
QFSSIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSR
Sbjct: 181 QFSSIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSR 360
Query: 652 LDDF*YSWYEWKSNPRICLLSI* 674
LDDF*YSWYEWKSNPRICLLSI*
Sbjct: 361 LDDF*YSWYEWKSNPRICLLSI* 429
>AV412895
Length = 405
Score = 237 bits (604), Expect = 5e-63
Identities = 117/135 (86%), Positives = 122/135 (89%)
Frame = +1
Query: 477 EGGFSFNSSFVFLQDQYLEEPDPVFNWIWMVPVPSNIKAFVWRVLLGRIQTRDNLLN*QV 536
+GGFS NSSFVFL DQYLEEPDPVFN IWMVP P NIKAFVWRVLLGRIQTRDNLL QV
Sbjct: 1 QGGFSVNSSFVFL*DQYLEEPDPVFN*IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQV 180
Query: 537 LHNALEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQFSSI 596
+HNAL+AICPLC LAEESGSHLLFSC+ SMLI YEC AWLGVSTAQVSDPKVHLLQFSSI
Sbjct: 181 IHNALDAICPLCGLAEESGSHLLFSCAESMLIWYECFAWLGVSTAQVSDPKVHLLQFSSI 360
Query: 597 GWSKAQKLGESAIWM 611
G SKAQKLGE+ IWM
Sbjct: 361 GRSKAQKLGETTIWM 405
>TC13572
Length = 534
Score = 145 bits (365), Expect(2) = 2e-38
Identities = 69/161 (42%), Positives = 96/161 (58%), Gaps = 4/161 (2%)
Frame = -3
Query: 344 WCRVVKANCNSCG---ESSWWKEIQSITV-DNGWPWFQESVQKTVFAGNATQFWAENWTG 399
WCRVV A G SSWW+++Q+ +NGW F+E V K + +GN T+FW ENWTG
Sbjct: 478 WCRVVLAKYGDGGCTNISSWWRDVQTAGGGENGW--FEEGVHKVLRSGNHTRFWLENWTG 305
Query: 400 SGSLELRFRRLYNLSCQKREQVSNMGKWVNGVWQWEFKWRRNLQGREVGWFEELMVILQG 459
G L ++ RLYNLS K + G W GVW W+F+WRR L GRE+ W + L + +
Sbjct: 304 VGILREKYYRLYNLSKLKWASIDECGGWSQGVWNWDFRWRRPLAGRELDWLQALSLDIVR 125
Query: 460 IRLVENKKDTWRWKLEGEGGFSFNSSFVFLQDQYLEEPDPV 500
+ L+ D W WK +G +S NS+F+FLQ + P+P+
Sbjct: 124 VPLLAGVPDKWVWKPSEDGSYSVNSAFIFLQVPSEQPPNPI 2
Score = 31.6 bits (70), Expect(2) = 2e-38
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -1
Query: 325 NMALLGKWKWRFLTEPDSL 343
N ALLGKW WR T P+SL
Sbjct: 534 NKALLGKWLWRLKTVPNSL 478
>BP041113
Length = 503
Score = 152 bits (385), Expect = 1e-37
Identities = 78/170 (45%), Positives = 104/170 (60%)
Frame = +1
Query: 392 FWAENWTGSGSLELRFRRLYNLSCQKREQVSNMGKWVNGVWQWEFKWRRNLQGREVGWFE 451
FW E+W G G+L R+ RL+NLS +KR + G W +GVW W+ WRR L GRE+GW E
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLSKEKRCCILECGGWNHGVWTWKLSWRRALVGRELGWLE 180
Query: 452 ELMVILQGIRLVENKKDTWRWKLEGEGGFSFNSSFVFLQDQYLEEPDPVFNWIWMVPVPS 511
+M L G+ L E +D W W L G G ++ NS++ FLQ L + DP+F IW PS
Sbjct: 181 LMMKDLSGVCLSEGVEDKWLW-LPG-GTYTVNSAYSFLQAPTLADTDPIFATIWRTVAPS 354
Query: 512 NIKAFVWRVLLGRIQTRDNLLN*QVLHNALEAICPLCCLAEESGSHLLFS 561
+KAF WR LLGR+ T DNL+ QV+ + +C C ES +HLLF+
Sbjct: 355 -VKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESVTHLLFA 501
>AV418280
Length = 398
Score = 119 bits (297), Expect = 2e-27
Identities = 55/128 (42%), Positives = 73/128 (56%)
Frame = -2
Query: 545 CPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQFSSIGWSKAQKL 604
CP C EE HL F+C SM + H WLG+S A + + QFS +K Q+L
Sbjct: 388 CPFCTTVEECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLANFAQFSITARNKNQRL 209
Query: 605 GESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEWKS 664
GE AIW++ +WS+W RN ++F LD LL+ IQ +W WL+++ F YS YEWKS
Sbjct: 208 GELAIWIATVWSLWIQRNSIIFRNNALDHSYLLDLIQSRSWHWLKAKFCGFTYSLYEWKS 29
Query: 665 NPRICLLS 672
P CL S
Sbjct: 28 CPLECLSS 5
>AV767121
Length = 525
Score = 115 bits (288), Expect = 2e-26
Identities = 67/150 (44%), Positives = 88/150 (58%), Gaps = 19/150 (12%)
Frame = +2
Query: 281 LLFHYFFYLFINYQLGCCQFVLERFL*GGAEADNKIS-----------------IKD*RL 323
L F FF L I C + ++ FL GG+E +NKI+ IKD
Sbjct: 80 LFFLSFFKLPIGVGKSCVR-LMRNFLWGGSENENKIA*VKWTDVCKPKELGGLGIKDLFT 256
Query: 324 FNMALLGKWKWRFLTEPDSLWCRVVKANCN--SCGESSWWKEIQSITVDNGWPWFQESVQ 381
FN ALLGK +WR+LTEPDSLW RV++A + SCG SSWW +I S+ ++ WF ++
Sbjct: 257 FNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYSCG-SSWWNDILSLCPEDADGWFSSGLK 433
Query: 382 KTVFAGNATQFWAENWTGSGSLELRFRRLY 411
K V G+ T+FW+E+W GSG L RFRRLY
Sbjct: 434 KLVGEGDQTKFWSEDWLGSGLLSARFRRLY 523
>BP056597
Length = 414
Score = 94.7 bits (234), Expect = 4e-20
Identities = 46/125 (36%), Positives = 66/125 (52%)
Frame = +3
Query: 407 FRRLYNLSCQKREQVSNMGKWVNGVWQWEFKWRRNLQGREVGWFEELMVILQGIRLVENK 466
F L+ +S Q+ ++ MG W+ W W+F W+ + G +V + I+ L+ K
Sbjct: 9 FDSLFLISNQQNCKIVEMGHWLENKWVWDFSWKNPISGEDVLKLGVMKQIVSSFSLINGK 188
Query: 467 KDTWRWKLEGEGGFSFNSSFVFLQDQYLEEPDPVFNWIWMVPVPSNIKAFVWRVLLGRIQ 526
KDTW W LEG+G +S S++ L VF+ +W PSN A WRV L RIQ
Sbjct: 189 KDTWVWILEGDGQYSVKSAYDLLSGLDTTIGISVFSKLWKACAPSNAVALGWRVFLDRIQ 368
Query: 527 TRDNL 531
T+DNL
Sbjct: 369 TKDNL 383
>AV414139
Length = 309
Score = 92.0 bits (227), Expect = 3e-19
Identities = 40/102 (39%), Positives = 60/102 (58%)
Frame = -2
Query: 421 VSNMGKWVNGVWQWEFKWRRNLQGREVGWFEELMVILQGIRLVENKKDTWRWKLEGEGGF 480
+ MG+W NGVW+WEF+WRR L RE +L+ L G+ L + +D W+W L+ E +
Sbjct: 308 IGGMGQWHNGVWEWEFRWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIY 129
Query: 481 SFNSSFVFLQDQYLEEPDPVFNWIWMVPVPSNIKAFVWRVLL 522
+ S++ L D + V+ +W+ PSN AFVWR+LL
Sbjct: 128 TVRSAY*LLLDPIADSDSEVYEKVWVGIAPSNANAFVWRLLL 3
>AV765930
Length = 576
Score = 86.3 bits (212), Expect = 2e-17
Identities = 40/40 (100%), Positives = 40/40 (100%)
Frame = -2
Query: 667 RICLLSI*SVGCHGVDCWVNCLPKVLINMTSNLVLRFKKL 706
RICLLSI*SVGCHGVDCWVNCLPKVLINMTSNLVLRFKKL
Sbjct: 575 RICLLSI*SVGCHGVDCWVNCLPKVLINMTSNLVLRFKKL 456
>AU089120
Length = 586
Score = 62.0 bits (149), Expect(2) = 1e-16
Identities = 31/88 (35%), Positives = 43/88 (48%)
Frame = +2
Query: 541 LEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQFSSIGWSK 600
++ + C +EES HL F C S + H WL + + K+H + F +
Sbjct: 26 IQTLSSFCLHSEESIPHLFFRCFQSWNLWSSVHRWLVFTVVTPEEGKMHFIMF*DYYSGR 205
Query: 601 AQKLGESAIWMSVLWSIWCLRNMVVFNG 628
K G IW+S +W IW LRN VVFNG
Sbjct: 206 DIKNGLGFIWLSTIWFIWHLRNKVVFNG 289
Score = 41.6 bits (96), Expect(2) = 1e-16
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +3
Query: 637 LEQIQVTAWRWLRSRLDDF*YSWYEWKSNPRICL 670
L+ IQ +W WL+ ++ +S YEW SNP +CL
Sbjct: 315 LDSIQYRSWLWLKGKVKGLCFSLYEWSSNPLVCL 416
>AU089037
Length = 245
Score = 78.2 bits (191), Expect = 4e-15
Identities = 33/63 (52%), Positives = 43/63 (67%)
Frame = -1
Query: 500 VFNWIWMVPVPSNIKAFVWRVLLGRIQTRDNLLN*QVLHNALEAICPLCCLAEESGSHLL 559
+F +W VP PSN +F WR++L R+QTR NL QV+ + EA+CP+C EES SHL
Sbjct: 203 IFQKLWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLF 24
Query: 560 FSC 562
FSC
Sbjct: 23 FSC 15
>BP057427
Length = 480
Score = 76.6 bits (187), Expect = 1e-14
Identities = 38/92 (41%), Positives = 57/92 (61%)
Frame = -1
Query: 585 DPKVHLLQFSSIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTA 644
+ V+ LQF + ++ G +IW++ L SIW RN ++F G ELD +L++QIQ +
Sbjct: 471 EASVNFLQFMQGIGNLKRRRGAWSIWVAALASIWYSRNSLIFKGEELDFQRLVDQIQTKS 292
Query: 645 WRWLRSRLDDF*YSWYEWKSNPRICLLSI*SV 676
W WL++++ F YS EWKS P CL I*S+
Sbjct: 291 WSWLKAKVTGFSYSLIEWKSQPLSCLDII*SL 196
>TC15601
Length = 613
Score = 75.5 bits (184), Expect = 3e-14
Identities = 30/72 (41%), Positives = 49/72 (67%)
Frame = +2
Query: 603 KLGESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEW 662
+L + +++ W++W LRNM++F G LD ++++E Q+ AW WLR++ DF +S YEW
Sbjct: 65 QLNSDEVCIAIAWTLWNLRNMIIFKEGHLDGERVVEIAQLRAWNWLRAKKKDFKFSTYEW 244
Query: 663 KSNPRICLLSI* 674
NPR C+ S+*
Sbjct: 245 VVNPRYCIGSL* 280
>AV428268
Length = 429
Score = 75.5 bits (184), Expect = 3e-14
Identities = 52/144 (36%), Positives = 73/144 (50%), Gaps = 1/144 (0%)
Frame = +3
Query: 480 FSFNSSFVFLQDQYLEEPDPVFNWIWMVPVPSNIKAFVWRVLLGRIQTRDNLLN*QVLHN 539
+S S++ FL + D +F +W V VPSN A W+VL+ R+QT+ NL V+
Sbjct: 6 YSVKSAYDFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVV-- 179
Query: 540 ALEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQ-FSSIGW 598
+ +CPLC L EES HLLFSC I + GV + +D H LQ S G
Sbjct: 180 -ISNVCPLCSLDEESTDHLLFSCPIV*RIWSKISE*FGVFSVFPNDSHGHFLQHLGSCGS 356
Query: 599 SKAQKLGESAIWMSVLWSIWCLRN 622
+K G +W++ + IW RN
Sbjct: 357 LNFRKRG-WFVWIAAVVCIWQGRN 425
>TC20217
Length = 561
Score = 73.2 bits (178), Expect = 1e-13
Identities = 55/189 (29%), Positives = 89/189 (46%), Gaps = 5/189 (2%)
Frame = +1
Query: 455 VILQGIRLVENKKDTWRWKLEGEGGFSFNSSFVFLQDQ--YLEEP--DPVFNWIWMVPVP 510
++L + V + D W W +G +F + YL+ P + +W + +
Sbjct: 1 IMLDEAKSVPLEPDKWCWLPSTDGILQLTLFILFFSHRCCYLQIPFSSSEYGSLWQLQM* 180
Query: 511 SNIKAFVWRVLLGRIQTRDNLLN*QVL-HNALEAICPLCCLAEESGSHLLFSCSNSMLI* 569
+ F+ RV+L I NL V N L + C + LLFSC S+ I
Sbjct: 181 ---RPFL*RVMLDWIPWLQNLWKHCVS*RNNL*GVAFGCGIL*A----LLFSCPVSLDIW 339
Query: 570 YECHAWLGVSTAQVSDPKVHLLQFSSIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGG 629
C+ W+GV T +P+ HLL F G +K + G AIW++V+W++W +RN ++F G
Sbjct: 340 RHCYRWMGVCTTLPRNPRQHLL*FQ-FGGNKKHQRGADAIWLAVIWTLWLIRNEIIFRNG 516
Query: 630 ELDKDQLLE 638
LD ++E
Sbjct: 517 MLDIPAVME 543
>TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular
solute-binding family 3 protein, partial (3%)
Length = 503
Score = 72.4 bits (176), Expect(2) = 2e-13
Identities = 36/103 (34%), Positives = 56/103 (53%)
Frame = +1
Query: 575 WLGVSTAQVSDPKVHLLQFSSIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGGELDKD 634
WLGV P HLL F+ + + AQK G ++W++V+W +W RN VF G +
Sbjct: 112 WLGVCFVCPGTPVDHLLSFAGL-FLLAQKDGILSVWLAVVWHVWVGRNEAVFREGVFLPE 288
Query: 635 QLLEQIQVTAWRWLRSRLDDF*YSWYEWKSNPRICLLSI*SVG 677
+ +++ W WL++R F ++ YEW + P +CLL * G
Sbjct: 289 CIFYLVKLKVWEWLKARSCHFTHATYEWFNEPILCLLDY*MEG 417
Score = 20.4 bits (41), Expect(2) = 2e-13
Identities = 9/22 (40%), Positives = 12/22 (53%)
Frame = +2
Query: 541 LEAICPLCCLAEESGSHLLFSC 562
+++ CPL ES HL F C
Sbjct: 11 VDSSCPLRGAYNESILHLFFFC 76
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 72.0 bits (175), Expect = 3e-13
Identities = 33/90 (36%), Positives = 51/90 (56%)
Frame = -1
Query: 416 QKREQVSNMGKWVNGVWQWEFKWRRNLQGREVGWFEELMVILQGIRLVENKKDTWRWKLE 475
QK E + N+GK V GVW W F RRN RE +L +L+ + L E+ D+W W
Sbjct: 816 QKNELILNLGKRVEGVWTWSFIRRRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTAN 637
Query: 476 GEGGFSFNSSFVFLQDQYLEEPDPVFNWIW 505
EG ++ +++ FL+ E DP+F+++W
Sbjct: 636 PEGQYTVQTAYKFLRASSNEFTDPIFHFVW 547
>AV780221
Length = 461
Score = 71.6 bits (174), Expect = 4e-13
Identities = 34/88 (38%), Positives = 47/88 (52%), Gaps = 4/88 (4%)
Frame = +3
Query: 316 ISIKD*RLFNMALLGKWKWRFLTEPDSLWCRVV---KANCNSCGESSWWKEIQSITV-DN 371
+ +++ LFN ALLGKW+WR L E D LW +V+ N SSWW ++ S D
Sbjct: 195 LGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYKNAIPQSASSWWNDLHSTCFEDG 374
Query: 372 GWPWFQESVQKTVFAGNATQFWAENWTG 399
G W Q + + + G +FW ENW G
Sbjct: 375 GGGWMQRGLCRRIGEGTEVKFWNENWLG 458
>TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, partial (5%)
Length = 579
Score = 70.1 bits (170), Expect = 1e-12
Identities = 29/64 (45%), Positives = 46/64 (71%)
Frame = +3
Query: 611 MSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEWKSNPRICL 670
M+ +WS+ +RN ++F+GG LDK +L+ I +W WL+S++D F S +EWK+NP IC+
Sbjct: 15 MATVWSLXLVRNSILFSGGVLDKANILDSIPWRSWFWLKSKMDGFTSSLFEWKTNPLICI 194
Query: 671 LSI* 674
S+*
Sbjct: 195 QSL* 206
>BP033775
Length = 530
Score = 53.1 bits (126), Expect(2) = 1e-12
Identities = 22/50 (44%), Positives = 33/50 (66%)
Frame = -2
Query: 621 RNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEWKSNPRICL 670
RN V+FNG ++ + +++ I+ AW+WL+ + DF YS Y W NP ICL
Sbjct: 394 RNDVLFNGKTINSEAVVDLIKFKAWKWLQCKTKDFSYSSYAWNWNPCICL 245
Score = 37.0 bits (84), Expect(2) = 1e-12
Identities = 18/46 (39%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
Frame = -1
Query: 575 WLGVSTAQVSDPKVHLLQFSSIGWSKAQKLGES--AIWMSVLWSIW 618
WLGV T Q DP+ H F G + Q LG +W + +W+IW
Sbjct: 530 WLGVITVQEEDPRRH---FDQHGSTLVQSLGSGWWTVWCAGIWTIW 402
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.345 0.151 0.582
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,615,888
Number of Sequences: 28460
Number of extensions: 313264
Number of successful extensions: 3972
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 3779
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3943
length of query: 706
length of database: 4,897,600
effective HSP length: 97
effective length of query: 609
effective length of database: 2,136,980
effective search space: 1301420820
effective search space used: 1301420820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0330.6