BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.8
(250 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10233 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (32%) 238 7e-64
BI418831 145 8e-36
TC19156 similar to GB|AAL91278.1|19699336|AY090375 At2g15220/F15... 124 2e-29
AU252027 54 3e-08
AV417847 37 0.003
AV768479 32 0.10
CB828734 30 0.51
TC16123 similar to UP|PGC2_HUMAN (O15173) Membrane associated pr... 28 1.1
TC7931 weakly similar to UP|Q43640 (Q43640) Dioxygenase, partial... 28 1.5
TC18894 27 4.3
TC8414 27 4.3
TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcrip... 27 4.3
TC8415 weakly similar to UP|CYG4_HUMAN (P33402) Guanylate cyclas... 26 5.7
TC11295 similar to UP|Q9LUC6 (Q9LUC6) Cytochrome P450 (AT3g14680... 26 5.7
BI418438 26 5.7
TC14092 weakly similar to UP|Q94KE6 (Q94KE6) Dihydro-flavanoid r... 26 7.4
CB828185 25 9.7
TC14184 similar to UP|Q93WZ6 (Q93WZ6) Abscisic stress ripening-l... 25 9.7
TC15058 25 9.7
>TC10233 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (32%)
Length = 889
Score = 238 bits (607), Expect = 7e-64
Identities = 112/223 (50%), Positives = 153/223 (68%)
Frame = +1
Query: 28 IYLMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTF 87
++ + L+I ++ AV Y VTN+A TPGG RF ++IG +QT+ A+QFI + F
Sbjct: 37 MHFIGLLIFLTAMQGTKAVDYTVTNKALSTPGGVRFRDQIGEASARQTLDSATQFIWRIF 216
Query: 88 KQIDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEA 147
+Q KN+E VS+ + ++G A TS+N I L + Y+ + GD++ E+ G+LYHE
Sbjct: 217 QQNTAADRKNVEKVSLFIDDMDG--VAYTSNNEIHLSARYVNSYGGDLKREISGVLYHEM 390
Query: 148 THIWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGWDEGYVVTAYFLDYCNG 207
H+WQW GN AP GL EG+AD++RLKA +AP HWV G G+ WD GY VTA FLDYCN
Sbjct: 391 VHVWQWNGNGGAPGGLIEGIADYVRLKADYAPSHWVKPGQGNKWDHGYDVTARFLDYCNS 570
Query: 208 LRDGFVAMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKSVFGN 250
L++GFVA LN M+ YSD FFV++LGKSVDQLW+DYK+ +GN
Sbjct: 571 LKNGFVAELNKKMRTGYSDQFFVQILGKSVDQLWTDYKAKYGN 699
>BI418831
Length = 561
Score = 145 bits (365), Expect = 8e-36
Identities = 68/170 (40%), Positives = 107/170 (62%)
Frame = +2
Query: 28 IYLMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTF 87
++++ L++ ++ AV Y VTN A T GG RF ++IG + +QT+ A+QF + F
Sbjct: 53 MHILCLLMFLTAMQGTKAVDYTVTNNALTTAGGARFRDQIGAEYAKQTLDSATQFTWRVF 232
Query: 88 KQIDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEA 147
+Q +N++ VS+ V ++G + S++ I L + Y+ +SGD++ E+ G+LYHE
Sbjct: 233 QQNTPADRRNVQTVSLFVDDMDGVAYTTPSNSQIHLSARYVNSYSGDLKREITGVLYHEI 412
Query: 148 THIWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGWDEGYVV 197
H+WQ G AP GL EG+AD++RLKA +AP HWV G G+ WD+GY V
Sbjct: 413 VHVWQ-NGIGSAPGGLIEGIADYVRLKANYAPSHWVKPGQGNKWDQGYDV 559
>TC19156 similar to GB|AAL91278.1|19699336|AY090375 At2g15220/F15A23.4
{Arabidopsis thaliana;}, partial (41%)
Length = 479
Score = 124 bits (310), Expect = 2e-29
Identities = 58/93 (62%), Positives = 70/93 (74%), Gaps = 1/93 (1%)
Frame = +1
Query: 155 GNKEAPRGLTEGVADFMRLKAGFAPPH-WVPRGTGSGWDEGYVVTAYFLDYCNGLRDGFV 213
GN +AP GL EG+ADF+RLKAG+AP WVP G G WD+GY VTA FLDYCN +R+GFV
Sbjct: 4 GNGQAPSGLIEGIADFVRLKAGYAPLDLWVPPGGGDKWDQGYDVTARFLDYCNSIRNGFV 183
Query: 214 AMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKS 246
LN MK YS+ +F +LLGKSV QLW+ YK+
Sbjct: 184 GELNKKMKSSYSETYFTQLLGKSVTQLWTGYKA 282
>AU252027
Length = 363
Score = 53.9 bits (128), Expect = 3e-08
Identities = 32/100 (32%), Positives = 59/100 (59%), Gaps = 9/100 (9%)
Frame = +2
Query: 29 YLMFLVIIASSGSPIHA-VQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTF 87
Y + +++ +P+H V+Y V N A T GG RF+ ++G+ + + T+ A++FIL+ F
Sbjct: 59 YGLCFIVLTLVVAPVHGEVEYSVLNNAESTIGGQRFDRQVGVDYAKDTMSSATEFILKLF 238
Query: 88 KQIDQFG----GKNIEHVSVIV-TALNG---NSAAITSDN 119
+Q + KN++ VS+IV +++G + AA S+N
Sbjct: 239 EQHNSTSVAADRKNVQKVSMIVEISIHGDDVDDAAYCSNN 358
>AV417847
Length = 432
Score = 37.0 bits (84), Expect = 0.003
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +3
Query: 121 IFLDSNYIEGFSGDVRNEVIGILYHEATHIWQ 152
++++ + F G ++ E+ G+LYHE H+WQ
Sbjct: 336 LWIEPRNLNNFDGSLKREISGVLYHEMGHVWQ 431
>AV768479
Length = 462
Score = 32.0 bits (71), Expect = 0.10
Identities = 27/90 (30%), Positives = 39/90 (43%), Gaps = 10/90 (11%)
Frame = -2
Query: 155 GNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGT-------GSGW--DEGYVVTAYFLDYC 205
G AP G +G+A+++ AGF P +P G W D+ A L YC
Sbjct: 458 GGFRAPIGFFDGMAEYITKVAGF-PRKGLPECGQKEEALGGLRWWEDKEPAHVARLLHYC 282
Query: 206 NGL-RDGFVAMLNAMMKDHYSDDFFVKLLG 234
+ GF+ LN M+D + D +LG
Sbjct: 281 EDYYKKGFIRRLNETMRDTWHDRVVENVLG 192
>CB828734
Length = 445
Score = 29.6 bits (65), Expect = 0.51
Identities = 12/33 (36%), Positives = 23/33 (69%), Gaps = 2/33 (6%)
Frame = +2
Query: 3 LKLP--ICCILSSCAALIYHWTKMSPIIYLMFL 33
L++P +C I+++C L+ WT + P++ L+FL
Sbjct: 311 LQMPYYVCAIVNTCFKLLQIWTFLIPMVKLVFL 409
>TC16123 similar to UP|PGC2_HUMAN (O15173) Membrane associated progesterone
receptor component 2 (Progesterone membrane binding
protein) (Steroid receptor protein DG6), partial (15%)
Length = 604
Score = 28.5 bits (62), Expect = 1.1
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Frame = -2
Query: 78 LASQFILQTFKQIDQFGGKNIE---HVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGD 134
L+ + +L + D F G+ IE + +++ L SA ++S + + +EGFSG
Sbjct: 360 LSYELVLPVIENADLFVGEAIEVGGDIFLVLAHLR*GSAGVSSGEHRVSTTGAVEGFSG- 184
Query: 135 VRNEVIGILYHEATH 149
G + H A H
Sbjct: 183 ------GDIEHAALH 157
>TC7931 weakly similar to UP|Q43640 (Q43640) Dioxygenase, partial (29%)
Length = 1477
Score = 28.1 bits (61), Expect = 1.5
Identities = 22/78 (28%), Positives = 38/78 (48%), Gaps = 8/78 (10%)
Frame = -2
Query: 94 GGKNIEHVSVIVTALNGNSAAITSDNNIFLDS--NYIEGFSGDV------RNEVIGILYH 145
G K +E++ I+ L GNS + +IFL S N ++GFS + R+ + G
Sbjct: 288 GVKFLENI*CILD*LIGNSMVDNLEKSIFLGSFKNLLQGFSTIMSTEIHHRDRLAGSNSD 109
Query: 146 EATHIWQWFGNKEAPRGL 163
A ++W ++ RG+
Sbjct: 108 RAWPTFRWLNIRQGHRGM 55
>TC18894
Length = 507
Score = 26.6 bits (57), Expect = 4.3
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = -1
Query: 22 TKMSPIIYLMFLVIIASSGSPIHAVQYQVTN 52
TK I+YL + + IA++ PIH + QV N
Sbjct: 420 TKTLWILYLFYWIAIATNLIPIHTLTGQVLN 328
>TC8414
Length = 1363
Score = 26.6 bits (57), Expect = 4.3
Identities = 13/29 (44%), Positives = 16/29 (54%)
Frame = +1
Query: 173 LKAGFAPPHWVPRGTGSGWDEGYVVTAYF 201
LK GF+ W TGS WDEG + +F
Sbjct: 379 LKNGFSVWEW----TGSAWDEGSEASQWF 453
>TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcription
factor 53 (WRKY DNA-binding protein 53), partial (15%)
Length = 1044
Score = 26.6 bits (57), Expect = 4.3
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 118 DNNIFLDSNYIEGFS 132
DNNIF ++N+IE FS
Sbjct: 387 DNNIFSENNFIESFS 431
>TC8415 weakly similar to UP|CYG4_HUMAN (P33402) Guanylate cyclase soluble,
alpha-2 chain (GCS-alpha-2) , partial (3%)
Length = 548
Score = 26.2 bits (56), Expect = 5.7
Identities = 14/38 (36%), Positives = 17/38 (43%), Gaps = 6/38 (15%)
Frame = +1
Query: 173 LKAGFAPPHWVPRG------TGSGWDEGYVVTAYFLDY 204
LK H V G +GS WDEG + +F DY
Sbjct: 388 LKVSLNKQHEVANGLSVWEWSGSAWDEGAEASQWFSDY 501
>TC11295 similar to UP|Q9LUC6 (Q9LUC6) Cytochrome P450 (AT3g14680/MIE1_18),
partial (24%)
Length = 544
Score = 26.2 bits (56), Expect = 5.7
Identities = 13/44 (29%), Positives = 20/44 (44%)
Frame = +2
Query: 142 ILYHEATHIWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPR 185
+L H +IW + P +EG+A + + F P W PR
Sbjct: 107 LLIHHDRNIWGDDATEFEPERFSEGLAKATKGQVSFFPFGWGPR 238
>BI418438
Length = 431
Score = 26.2 bits (56), Expect = 5.7
Identities = 14/43 (32%), Positives = 25/43 (57%)
Frame = +3
Query: 3 LKLPICCILSSCAALIYHWTKMSPIIYLMFLVIIASSGSPIHA 45
LK+PI ++ + I + MSP++ L FL++I + S H+
Sbjct: 90 LKVPIKPTTTTFSLTISTFLTMSPLLPLSFLLLILTLSSASHS 218
>TC14092 weakly similar to UP|Q94KE6 (Q94KE6) Dihydro-flavanoid
reductase-like protein, partial (32%)
Length = 721
Score = 25.8 bits (55), Expect = 7.4
Identities = 7/14 (50%), Positives = 13/14 (92%)
Frame = +3
Query: 12 SSCAALIYHWTKMS 25
S C+++I+HWTK++
Sbjct: 33 SDCSSIIHHWTKLA 74
>CB828185
Length = 533
Score = 25.4 bits (54), Expect = 9.7
Identities = 14/38 (36%), Positives = 20/38 (51%)
Frame = +2
Query: 154 FGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGW 191
FGN E G + D + AGF H++P+G+ S W
Sbjct: 266 FGNAE--NGASH---DSRSMLAGFGSVHFIPQGSSSLW 364
>TC14184 similar to UP|Q93WZ6 (Q93WZ6) Abscisic stress ripening-like
protein, partial (57%)
Length = 1141
Score = 25.4 bits (54), Expect = 9.7
Identities = 11/24 (45%), Positives = 13/24 (53%)
Frame = +3
Query: 184 PRGTGSGWDEGYVVTAYFLDYCNG 207
P G G+D GY TAY D +G
Sbjct: 297 PNSGGGGYDSGYNKTAYSTDEPSG 368
>TC15058
Length = 716
Score = 25.4 bits (54), Expect = 9.7
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +1
Query: 1 MNLKLPICCILSSCAALIYHWTKMSPIIYLMFLVII 36
M ++ +CC+ AL Y W I+Y + L++I
Sbjct: 181 MTARIEVCCMWRQLIALDYCW-----ILYRLLLILI 273
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.139 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,453,272
Number of Sequences: 28460
Number of extensions: 56937
Number of successful extensions: 337
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 334
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 334
length of query: 250
length of database: 4,897,600
effective HSP length: 88
effective length of query: 162
effective length of database: 2,393,120
effective search space: 387685440
effective search space used: 387685440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0327.8