BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0323a.9
(191 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP085408 79 4e-16
AW720061 52 1e-13
TC10838 weakly similar to PIR|T04232|T04232 pathogenesis-related... 62 8e-11
AV779946 60 2e-10
AV418386 28 0.77
TC17471 27 2.3
TC8737 similar to UP|UCRI_PEA (P26291) Cytochrome B6-F complex i... 27 2.9
BP069831 26 3.8
TC15629 similar to UP|Q40540 (Q40540) Pit2 protein, partial (21%) 26 3.8
TC15447 26 5.0
AV773398 26 5.0
TC14348 homologue to UP|Q9M4R0 (Q9M4R0) Ubiquitin conjugating pr... 26 5.0
AV769619 26 5.0
BP045216 25 6.6
BP085971 25 6.6
AV779795 25 6.6
BP063783 25 6.6
BP035291 25 8.6
TC9004 25 8.6
>BP085408
Length = 350
Score = 79.3 bits (194), Expect = 4e-16
Identities = 36/75 (48%), Positives = 48/75 (64%), Gaps = 1/75 (1%)
Frame = -1
Query: 118 PREVMRQWVKDR-FYYEGNNSCVPANYPCGDYTQIVWRKTKYLGCAQAECGPDYGGVRLN 176
P ++ WV ++ FY NSCV ++PC YTQ+VWRK+K +GCAQ C D + L
Sbjct: 305 PSVAVQDWVNEKEFYSYRTNSCVAPSFPCWHYTQVVWRKSKQVGCAQLTCVVD--KLILT 132
Query: 177 VCFYYPPGNVPGERP 191
+CFY PPGN+ GE P
Sbjct: 131 ICFYDPPGNINGESP 87
>AW720061
Length = 304
Score = 52.0 bits (123), Expect(2) = 1e-13
Identities = 29/70 (41%), Positives = 42/70 (59%), Gaps = 1/70 (1%)
Frame = +1
Query: 71 SDKLAKDASLYARFRRDKYLCTPGIFDKKRYGVCCNQWLEDTVKIMDPREVMRQWVKDR- 129
S+KL KDASL+ R++R+K+ C YG NQ + +V + PR + +WVK++
Sbjct: 4 SEKLGKDASLFVRYQRNKFGCGFANLTDSEYG--GNQIMAGSVSVA-PRVAVAEWVKEKD 174
Query: 130 FYYEGNNSCV 139
FY NNSCV
Sbjct: 175 FYIHANNSCV 204
Score = 39.3 bits (90), Expect(2) = 1e-13
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +3
Query: 145 CGDYTQIVWRKTKYLGCAQAEC 166
CG YTQ+VWR +K +GC+QA C
Sbjct: 216 CGVYTQVVWRDSKEVGCSQASC 281
>TC10838 weakly similar to PIR|T04232|T04232 pathogenesis-related protein
homolog F14M19.60 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (78%)
Length = 914
Score = 61.6 bits (148), Expect = 8e-11
Identities = 46/141 (32%), Positives = 64/141 (44%)
Frame = +3
Query: 51 YLFEHNFVRREKGISEHLEWSDKLAKDASLYARFRRDKYLCTPGIFDKKRYGVCCNQWLE 110
+LF HN VR K S L W +L A +A R+ F + + + N +
Sbjct: 375 FLFRHNMVRAAKWESP-LMWDFQLQSYARWWAGQRKPDCKVEHS-FPENDFKLGENIFW- 545
Query: 111 DTVKIMDPREVMRQWVKDRFYYEGNNSCVPANYPCGDYTQIVWRKTKYLGCAQAECGPDY 170
+ P + ++ W + YY + CG YTQIVW+ TK +GCA+ C D
Sbjct: 546 GSGSAWTPTDAVKAWADEEKYYTYATNTCEEGQMCGHYTQIVWKNTKRVGCARVVC--DD 719
Query: 171 GGVRLNVCFYYPPGNVPGERP 191
G V + C Y P GN GERP
Sbjct: 720 GDVFM-TCNYDPVGNYVGERP 779
>AV779946
Length = 533
Score = 60.1 bits (144), Expect = 2e-10
Identities = 32/75 (42%), Positives = 39/75 (51%), Gaps = 1/75 (1%)
Frame = -3
Query: 118 PREVMRQWVKDRFYYE-GNNSCVPANYPCGDYTQIVWRKTKYLGCAQAECGPDYGGVRLN 176
P + WV++R +Y +NSC CG YTQIVW T+ +GCA C G
Sbjct: 423 PSPAVDAWVEERQWYNYWHNSCANGEM-CGHYTQIVWGDTRKVGCASVTCSGGQG--TFM 253
Query: 177 VCFYYPPGNVPGERP 191
C Y PPGN GERP
Sbjct: 252 TCNYDPPGNYYGERP 208
>AV418386
Length = 352
Score = 28.5 bits (62), Expect = 0.77
Identities = 12/29 (41%), Positives = 18/29 (61%)
Frame = -2
Query: 155 KTKYLGCAQAECGPDYGGVRLNVCFYYPP 183
K ++ G A E G ++GGV L + +YPP
Sbjct: 207 KERHEGGAAEELGDEHGGVSLGLGAFYPP 121
>TC17471
Length = 556
Score = 26.9 bits (58), Expect = 2.3
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
Frame = -2
Query: 104 CCNQWLEDTVKIMDPREVMRQWVKDR-FYYEGNNSCVPANYP 144
C WLE+ + + RE + W K R F +G+ P YP
Sbjct: 198 CNPSWLENLLPSLG*RE*CKAWAKQRLFPLQGSRRR*PCRYP 73
>TC8737 similar to UP|UCRI_PEA (P26291) Cytochrome B6-F complex iron-sulfur
subunit, chloroplast precursor (Rieske iron-sulfur
protein) (RISP) , partial (97%)
Length = 974
Score = 26.6 bits (57), Expect = 2.9
Identities = 9/31 (29%), Positives = 15/31 (48%)
Frame = +3
Query: 136 NSCVPANYPCGDYTQIVWRKTKYLGCAQAEC 166
N V + PC +T + W++ +L A C
Sbjct: 114 NHVVHNSLPCNPFTAMFWKEWNFLSLTSASC 206
>BP069831
Length = 423
Score = 26.2 bits (56), Expect = 3.8
Identities = 10/30 (33%), Positives = 15/30 (49%)
Frame = +3
Query: 129 RFYYEGNNSCVPANYPCGDYTQIVWRKTKY 158
R Y + C+PA P Y+ +RKT +
Sbjct: 309 RIYLKSAILCIPATQPIPPYSSFCYRKTPF 398
>TC15629 similar to UP|Q40540 (Q40540) Pit2 protein, partial (21%)
Length = 616
Score = 26.2 bits (56), Expect = 3.8
Identities = 12/57 (21%), Positives = 29/57 (50%)
Frame = -1
Query: 25 FAVLVALALLLVSSQATPATTDGAKVYLFEHNFVRREKGISEHLEWSDKLAKDASLY 81
+ + + + L ++SSQA+ + + +N + ++ ++H+ WS KL S +
Sbjct: 613 YKLFI*IILYIISSQASNNSLHELVIN*INYNLLMKQNNTTQHINWS-KLGSRTSYF 446
>TC15447
Length = 665
Score = 25.8 bits (55), Expect = 5.0
Identities = 20/60 (33%), Positives = 27/60 (44%)
Frame = +3
Query: 16 KPKMFPHPFFAVLVALALLLVSSQATPATTDGAKVYLFEHNFVRREKGISEHLEWSDKLA 75
+PK P F+AV+ L +LL+ A P A+ N I + LEWS LA
Sbjct: 36 RPKWTPRIFYAVVAVLFILLLRHHA-PDEAARARYQKRVSNI------IDDVLEWSPSLA 194
>AV773398
Length = 496
Score = 25.8 bits (55), Expect = 5.0
Identities = 12/39 (30%), Positives = 20/39 (50%), Gaps = 6/39 (15%)
Frame = -1
Query: 128 DRFYYEGNNSCV------PANYPCGDYTQIVWRKTKYLG 160
D ++Y N +C+ P P G+Y + +WR+ LG
Sbjct: 295 DFYHYTQNMACLKCHTKRPQEPPSGEYEENIWRR*N*LG 179
>TC14348 homologue to UP|Q9M4R0 (Q9M4R0) Ubiquitin conjugating protein
(Ubiquitin-conjugating enzyme E2) (Ubiquitin-protein
ligase) (Ubiquitin carrier protein) , complete
Length = 886
Score = 25.8 bits (55), Expect = 5.0
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +2
Query: 20 FPHPFFAVLVALALLLVSSQATPA 43
FP PF + +AL L+V +TPA
Sbjct: 80 FPSPFSTLALALPFLVVVKMSTPA 151
>AV769619
Length = 499
Score = 25.8 bits (55), Expect = 5.0
Identities = 20/67 (29%), Positives = 30/67 (43%), Gaps = 9/67 (13%)
Frame = -3
Query: 103 VCCNQWLEDTVKIMDPREVMRQW--VKDRFY-YEGNNSCV------PANYPCGDYTQIVW 153
V C + LE +P++ W K FY Y N +C+ P P G+Y + +W
Sbjct: 377 VKCKRCLEPNQN-QNPKKYPGDWNCPKCDFYNYTKNMACLKCNTKRPKEQPSGEYEENIW 201
Query: 154 RKTKYLG 160
R+ LG
Sbjct: 200 RR*N*LG 180
>BP045216
Length = 487
Score = 25.4 bits (54), Expect = 6.6
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 129 RFYYEGNNSCVPANYPCGDYTQIVWRKTKYL 159
R YY+ +SCV PC +T W K+ +L
Sbjct: 164 RRYYDPCSSCVVVLVPC*FFTIWWWGKSNHL 72
>BP085971
Length = 500
Score = 25.4 bits (54), Expect = 6.6
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = -2
Query: 12 QHNAKPKMFPHPFFAVLVALAL 33
+ +KP FP PFF ++V + L
Sbjct: 355 EFGSKPLFFPFPFFMIVVVVLL 290
>AV779795
Length = 597
Score = 25.4 bits (54), Expect = 6.6
Identities = 15/67 (22%), Positives = 32/67 (47%), Gaps = 7/67 (10%)
Frame = +2
Query: 94 GIFDKKRYGVCCNQWLEDTVKIMDPREV-------MRQWVKDRFYYEGNNSCVPANYPCG 146
G+ ++RYG ++WL + ++ R + R WV + F + G CV ++
Sbjct: 326 GVMRRRRYG*DLSRWLHEDGRVRRERSMSG**EKDSRAWVDESFLHCG---CVLGDW**Q 496
Query: 147 DYTQIVW 153
+ +++W
Sbjct: 497 ESYRLLW 517
>BP063783
Length = 461
Score = 25.4 bits (54), Expect = 6.6
Identities = 10/30 (33%), Positives = 16/30 (53%), Gaps = 1/30 (3%)
Frame = -2
Query: 125 WVKDRFYYEGNNSCVPANYPCGDYTQI-VW 153
W D F++ C P +YP D+ Q+ +W
Sbjct: 94 WDGD*FFFFLFEGCKPVSYPTDDFIQL*IW 5
>BP035291
Length = 534
Score = 25.0 bits (53), Expect = 8.6
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 4/51 (7%)
Frame = +2
Query: 79 SLYARFRRDKYLCTPGIFDKKRYG----VCCNQWLEDTVKIMDPREVMRQW 125
S Y RF R ++L F R+G V CN WL T+K + V+R +
Sbjct: 275 SYYLRFCRKRFL-----FVYYRWGRSDRVDCNSWLMTTLKRLSIIVVLRMF 412
>TC9004
Length = 857
Score = 25.0 bits (53), Expect = 8.6
Identities = 16/50 (32%), Positives = 21/50 (42%)
Frame = +3
Query: 125 WVKDRFYYEGNNSCVPANYPCGDYTQIVWRKTKYLGCAQAECGPDYGGVR 174
W K+R ++E + PC Y+ I R K G CGP G R
Sbjct: 186 WAKERCFFEYHQKDC*VLMPCRHYSPIY*RGFK*DG-----CGPKVGNGR 320
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.142 0.477
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,378,926
Number of Sequences: 28460
Number of extensions: 69900
Number of successful extensions: 334
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 331
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 331
length of query: 191
length of database: 4,897,600
effective HSP length: 85
effective length of query: 106
effective length of database: 2,478,500
effective search space: 262721000
effective search space used: 262721000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0323a.9