BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0323a.7
(624 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV778581 206 4e-64
BP059135 128 3e-30
AV426226 82 2e-16
TC16280 80 1e-15
TC20223 similar to UP|Q8RY64 (Q8RY64) AT4g37080/C7A10_280, parti... 69 3e-12
AU252064 53 2e-07
TC10562 similar to UP|Q9FMZ5 (Q9FMZ5) Emb|CAB16786.1, partial (14%) 52 2e-07
BP064883 32 0.057
TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] ... 32 0.40
TC15284 similar to UP|Q8XX91 (Q8XX91) Probable transmembrane pro... 30 1.5
TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%) 29 2.6
TC13287 28 3.4
TC11199 28 4.4
BP054460 28 5.7
TC10877 similar to UP|O22840 (O22840) At2g43630 protein, partial... 28 5.7
BI421016 28 5.7
TC13505 similar to UP|Q9T0K5 (Q9T0K5) Extensin-like protein, par... 27 7.5
AV422058 27 7.5
TC10403 similar to UP|O80815 (O80815) T8F5.22 protein, partial (... 27 7.5
>AV778581
Length = 525
Score = 206 bits (523), Expect(2) = 4e-64
Identities = 101/102 (99%), Positives = 101/102 (99%)
Frame = -2
Query: 523 VRVYTPKRVFQELEVAKDEYIRATLGVRRKDQKVLVPKLVETFTKDSDMCPGGVMEMIQE 582
VRVYTPKRVFQELEVAKDEYIRATLGVRRKD KVLVPKLVETFTKDSDMCPGGVMEMIQE
Sbjct: 431 VRVYTPKRVFQELEVAKDEYIRATLGVRRKDPKVLVPKLVETFTKDSDMCPGGVMEMIQE 252
Query: 583 SLPESLRKSVKKCHQLAKSRKCIEWIPHNFTFRYLISKDVLK 624
SLPESLRKSVKKCHQLAKSRKCIEWIPHNFTFRYLISKDVLK
Sbjct: 251 SLPESLRKSVKKCHQLAKSRKCIEWIPHNFTFRYLISKDVLK 126
Score = 56.6 bits (135), Expect(2) = 4e-64
Identities = 24/28 (85%), Positives = 26/28 (92%)
Frame = -3
Query: 500 YAIENPEPLSHFALCSGNHSDPAVRVYT 527
YAIENPEPLSHFALCSGNHSDPA+ + T
Sbjct: 523 YAIENPEPLSHFALCSGNHSDPAILLIT 440
>BP059135
Length = 344
Score = 128 bits (321), Expect = 3e-30
Identities = 72/104 (69%), Positives = 81/104 (77%), Gaps = 2/104 (1%)
Frame = -2
Query: 378 WIYREGLKWGDIE-KLHQKFR-SLICRLEEVDPRKLKHEEKLAFWINIHNALVMHAFLAY 435
WIYR K G I +L Q F+ + + P KLKHEEKLAFWI+I +ALVMHAFLAY
Sbjct: 343 WIYRGE*KIGVIPAQLLQNFQVTNXVN*KR*XPGKLKHEEKLAFWIHIPHALVMHAFLAY 164
Query: 436 GIPQNNVKRVFLLLKAAYNVGGRTVSADTIQSTILRCRMSRPGQ 479
GI Q++VKRVFLLLKAAYN+GG TVSADTIQSTIL CRMSRPGQ
Sbjct: 163 GISQSSVKRVFLLLKAAYNIGGHTVSADTIQSTILGCRMSRPGQ 32
>AV426226
Length = 420
Score = 82.4 bits (202), Expect = 2e-16
Identities = 48/129 (37%), Positives = 69/129 (53%)
Frame = +2
Query: 416 KLAFWINIHNALVMHAFLAYGIPQNNVKRVFLLLKAAYNVGGRTVSADTIQSTILRCRMS 475
KLAF+IN++N + +HA L G P ++R L + Y +GG T S IQ+ ILR
Sbjct: 5 KLAFFINLYNMMAIHAILVLGHPDGALERRKLFGEFKYVIGGSTYSLSAIQNGILRGNQR 184
Query: 476 RPGQWLRMFFSSRTKFRSGDGRQTYAIENPEPLSHFALCSGNHSDPAVRVYTPKRVFQEL 535
P ++ F + D R A+ PEPL HFAL G S PA+R Y+P ++ +EL
Sbjct: 185 PP-------YNMTKPFGAKDRRSRVALPYPEPLIHFALVYGTRSGPALRCYSPGKIDEEL 343
Query: 536 EVAKDEYIR 544
A ++R
Sbjct: 344 LDAARNFLR 370
>TC16280
Length = 905
Score = 80.1 bits (196), Expect = 1e-15
Identities = 45/127 (35%), Positives = 66/127 (51%)
Frame = +1
Query: 416 KLAFWINIHNALVMHAFLAYGIPQNNVKRVFLLLKAAYNVGGRTVSADTIQSTILRCRMS 475
+LAF++N++NA+V+HA + G P++ + R Y VGG S I++ +LRC
Sbjct: 61 QLAFFLNLYNAMVIHAVIRVGCPESLIDRRSFFNDFLYLVGGHPYSLTDIKNGVLRCNRR 240
Query: 476 RPGQWLRMFFSSRTKFRSGDGRQTYAIENPEPLSHFALCSGNHSDPAVRVYTPKRVFQEL 535
P +S F +GD R A+ PL HF LC+G S P VR ++ RV EL
Sbjct: 241 TP-------YSLLKPFSAGDRRLEVALVKLNPLFHFGLCNGTKSSPNVRFFSSDRVVDEL 399
Query: 536 EVAKDEY 542
A E+
Sbjct: 400 RGAAREF 420
>TC20223 similar to UP|Q8RY64 (Q8RY64) AT4g37080/C7A10_280, partial (20%)
Length = 546
Score = 68.6 bits (166), Expect = 3e-12
Identities = 45/125 (36%), Positives = 66/125 (52%)
Frame = +2
Query: 399 LICRLEEVDPRKLKHEEKLAFWINIHNALVMHAFLAYGIPQNNVKRVFLLLKAAYNVGGR 458
L+ +L ++ + L H+EKLAFWIN +N+ +++A+L GIP++ V L+ KA VGG
Sbjct: 5 LLGKLASLNLKXLNHQEKLAFWINTYNSCMLNAYLEQGIPESPEMVVALMPKAIIVVGGP 184
Query: 459 TVSADTIQSTILRCRMSRPGQWLRMFFSSRTKFRSGDGRQTYAIENPEPLSHFALCSGNH 518
++A TI+ ILR L+ K R + +E EPL F L G
Sbjct: 185 LLNAITIEHFILRLPYH-----LKFTCP*AAKNEEVKARSIFGLEWSEPLVTFELSCGIW 349
Query: 519 SDPAV 523
S PAV
Sbjct: 350 SSPAV 364
>AU252064
Length = 300
Score = 52.8 bits (125), Expect = 2e-07
Identities = 32/90 (35%), Positives = 54/90 (59%)
Frame = +3
Query: 63 TLKQETLQLERRLQDQFEVRCTLEKALGYKSPSLVNSSEMIMPKPATELIKEIAVLEMEV 122
+L+++ QL+ RLQ + +R LE+A+G S +L + + +LI EI +LE EV
Sbjct: 3 SLEKDVEQLQLRLQQEKSMRNLLERAMGRASSTLSPGHRHVAGQ-TKDLIAEIELLEEEV 179
Query: 123 VYLEQHLLSLYRKAFDQQLSSVSPPTKEES 152
EQH+L++YR F+ +S PP+++ S
Sbjct: 180 TSREQHVLAMYRNIFEHCVS--QPPSEQNS 263
>TC10562 similar to UP|Q9FMZ5 (Q9FMZ5) Emb|CAB16786.1, partial (14%)
Length = 586
Score = 52.4 bits (124), Expect = 2e-07
Identities = 36/104 (34%), Positives = 56/104 (53%), Gaps = 7/104 (6%)
Frame = +2
Query: 523 VRVYTPKRVFQELEVAKDEYIRATLGVRRKDQKVLVPKLVE----TFTKDSDMCPGGVME 578
VRVYT +V ELEVAK EY++A +G+ + +PKL++ F KD + +++
Sbjct: 176 VRVYTASQVENELEVAKREYLQAAVGI--STSRFAIPKLLDWYLLNFAKDLE----SLLD 337
Query: 579 MIQESLPESLRKSVKKCHQLAKSR---KCIEWIPHNFTFRYLIS 619
I LP L K K+ + ++ +P+ F+FRYLIS
Sbjct: 338 WICLQLPSELGKEAINFLDKRKTEPLSQFVQIMPYEFSFRYLIS 469
>BP064883
Length = 370
Score = 32.0 bits (71), Expect(2) = 0.057
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = -2
Query: 598 LAKSRKCIEWIPHNFTFRYLISKDVLK 624
L S + +EW P +FTFRYL K+++K
Sbjct: 321 LPNSPEDLEWTPPHFTFRYLTPKELVK 241
Score = 21.2 bits (43), Expect(2) = 0.057
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -1
Query: 583 SLPESLRKSVKKC 595
++P LRK VKKC
Sbjct: 364 AVPAFLRKGVKKC 326
>TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(58%)
Length = 591
Score = 31.6 bits (70), Expect = 0.40
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 310 PPMTLPPGLLSPSSSLSSPSAFSIGDQGDMWSP 342
PP +PP +P+SS SSP + ++ WSP
Sbjct: 65 PPHLIPPPPSTPTSSSSSPLSSALSSASSAWSP 163
>TC15284 similar to UP|Q8XX91 (Q8XX91) Probable transmembrane protein,
partial (5%)
Length = 758
Score = 29.6 bits (65), Expect = 1.5
Identities = 30/127 (23%), Positives = 50/127 (38%), Gaps = 3/127 (2%)
Frame = -3
Query: 126 EQHLLSLYRKAFDQQLSSVSPPTKEESLKYPLTTPRAQVVKASL---PEVLTKEEYPTVQ 182
+ HLL +K F + S PP++ +L +T +++ K L P + + Y T+
Sbjct: 447 DYHLLICRQKYFGFAIWSSKPPSRNGNLITFTSTSQSKTTKFPLLEDPRCVNVKVYETIH 268
Query: 183 SNDHELEAMRKEHDRNELDNLRKECNGGWPEEKHLDSGVYRCHSSLSQYSAFTRSSPPAE 242
S++ E+ N P HL + V+ C +F S+P
Sbjct: 267 SSEDEISPW-------------FHTNWA*PHF*HLHASVHLCTQRTITQPSFA-SAPHCS 130
Query: 243 PLAKSLR 249
LA LR
Sbjct: 129 DLASCLR 109
>TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%)
Length = 1131
Score = 28.9 bits (63), Expect = 2.6
Identities = 16/38 (42%), Positives = 19/38 (49%), Gaps = 2/38 (5%)
Frame = +3
Query: 473 RMSRPGQWLRMFFSSRTKFRSGDG--RQTYAIENPEPL 508
R R G W FF +FRSGDG R A+ P+ L
Sbjct: 186 RQRRSGSW*WRFFPGTRRFRSGDGAVRLVRALLGPDRL 299
>TC13287
Length = 542
Score = 28.5 bits (62), Expect = 3.4
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +3
Query: 165 VKASLPEVLTKEEYPTVQSNDHELEAMRKEHDRNELDNLRKECNGGWPEEK 215
V A L + + +EEY +++ +E M KE +D L EC W ++K
Sbjct: 198 VSAWLSKHVKEEEYVVMKAEAEVVEEMIKERTIYLVDELFLECKNEWWQKK 350
>TC11199
Length = 743
Score = 28.1 bits (61), Expect = 4.4
Identities = 29/118 (24%), Positives = 45/118 (37%), Gaps = 4/118 (3%)
Frame = +1
Query: 237 SSPPAEPLAKSLRACHSQPLSMMEYARGCSSNIISLAEHLGTRISDHVLPVTPNKLSEDM 296
SSPP ++ ++P S + +++ SLA R T S
Sbjct: 121 SSPPPYATTRT*DPSSARPSSPASQSHSTTTSATSLAPK-SPRSKRSARRTTRTSSSPST 297
Query: 297 VKCISAIYCKLADPPMTLPPGLLSPSSSLSSPSAFSIGDQ----GDMWSPGFRNNSSF 350
+ S+ + PP P SPS +LSSP + + G SP R+ S+F
Sbjct: 298 ISDPSSPTSNPSSPPSLTPTPSSSPSLALSSPPSTPSSKRGMYPGTSTSPSTRSESAF 471
>BP054460
Length = 561
Score = 27.7 bits (60), Expect = 5.7
Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 4/65 (6%)
Frame = +2
Query: 239 PPAEPLAKSLRACHSQP-LSMMEYARGCSSNIISLAEHLGTR---ISDHVLPVTPNKLSE 294
PPA+ A H +P L ME+ +N + + +S+H+LP+TP L+
Sbjct: 2 PPAQSKASVPT*FHHRPILFTMEHGEAMMNNNLQMTLSSLNNLLDVSNHILPITPT-LNT 178
Query: 295 DMVKC 299
++++C
Sbjct: 179 NLIQC 193
>TC10877 similar to UP|O22840 (O22840) At2g43630 protein, partial (17%)
Length = 629
Score = 27.7 bits (60), Expect = 5.7
Identities = 23/58 (39%), Positives = 26/58 (44%), Gaps = 6/58 (10%)
Frame = -3
Query: 310 PPMTLPPGLLSPSSSLSSPSAFSIGDQGDMWSPGFRNNSSFDARLDNS------FHGE 361
PP LPPG L+P LS S F I R+ SS D L+ S FHGE
Sbjct: 501 PPPPLPPGGLAPLPPLSKYSPF*I---------CCRSTSSIDCPLNFSIAFSIDFHGE 355
>BI421016
Length = 507
Score = 27.7 bits (60), Expect = 5.7
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = -3
Query: 310 PPMTLPPGLLSPSSSLSSPSAFSI 333
PP TLPP LL+ S L SPS S+
Sbjct: 91 PPPTLPPPLLAFVSLLISPSQLSL 20
>TC13505 similar to UP|Q9T0K5 (Q9T0K5) Extensin-like protein, partial (7%)
Length = 359
Score = 27.3 bits (59), Expect = 7.5
Identities = 24/80 (30%), Positives = 36/80 (45%)
Frame = +1
Query: 248 LRACHSQPLSMMEYARGCSSNIISLAEHLGTRISDHVLPVTPNKLSEDMVKCISAIYCKL 307
LR+ P +M + C ++ LA + ++D L T S+D VKC C+L
Sbjct: 1 LRSFIVLPKTMGTTSPCCFITVVFLAA-IAVGLADQSLKTT----SKDEVKCTP---CEL 156
Query: 308 ADPPMTLPPGLLSPSSSLSS 327
A PP PP P + +S
Sbjct: 157 ASPPPPSPPPPSQPPPTPTS 216
>AV422058
Length = 442
Score = 27.3 bits (59), Expect = 7.5
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 237 SSPPAEPLAKSLRACHSQPLSM 258
S+PP+ P SL +C+ Q LSM
Sbjct: 358 SAPPSNPFLASLSSCYLQELSM 423
>TC10403 similar to UP|O80815 (O80815) T8F5.22 protein, partial (15%)
Length = 1392
Score = 27.3 bits (59), Expect = 7.5
Identities = 19/47 (40%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
Frame = -3
Query: 307 LADPPMTLPPGL-LSPSSSLSSPSAFSIGDQGDMWSPGFRNNSSFDA 352
LA PP LPP L P + S P F G + P F SFDA
Sbjct: 1057 LAPPPQPLPPSLPPQPPGNASLPGEF*TFAPGKLLKPSF---PSFDA 926
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,057,715
Number of Sequences: 28460
Number of extensions: 158224
Number of successful extensions: 975
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 968
length of query: 624
length of database: 4,897,600
effective HSP length: 96
effective length of query: 528
effective length of database: 2,165,440
effective search space: 1143352320
effective search space used: 1143352320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0323a.7