BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0320b.4
(161 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB827384 172 2e-44
AV415712 105 3e-24
TC12957 similar to UP|Q9FRY9 (Q9FRY9) Response regulator 6, part... 92 3e-20
BP073830 62 5e-11
BP079485 52 6e-08
TC8860 similar to UP|Q9C5T8 (Q9C5T8) Cytokinin receptor CRE1b, p... 47 2e-06
TC13528 similar to UP|Q9SXL4 (Q9SXL4) Histidine kinase 1, partia... 39 3e-04
BP055416 38 0.001
TC18201 homologue to GB|AAB86804.1|2454184|ATU80186 pyruvate deh... 28 0.76
TC8437 homologue to GB|AAB86804.1|2454184|ATU80186 pyruvate dehy... 28 0.99
TC19121 similar to UP|ENAH_MOUSE (Q03173) Enabled protein homolo... 27 2.2
TC18633 similar to GB|AAB94051.1|2736075|AF016644 PAP-specific p... 26 2.9
BP044479 25 8.4
>CB827384
Length = 548
Score = 172 bits (437), Expect = 2e-44
Identities = 87/134 (64%), Positives = 113/134 (83%), Gaps = 2/134 (1%)
Frame = +2
Query: 12 VMESGDIPHVLAVDDNLIDRKLVEKLLRNSSCKVTTAENGPRALEFLGLTSGEQNTLNGR 71
V+E D HVLAVDD+L+DRK++E+LL+ SSCKVT ++G RAL++LGL GE+N+ G
Sbjct: 104 VLEDSDELHVLAVDDSLVDRKVIERLLKISSCKVTVVDSGTRALQYLGL-DGEENSSIGF 280
Query: 72 S--KVNLIITDYCMPGMTGYELLKKIKESSAMKEVPVVIMSSENIPTRINKCLEEGAQMF 129
KVNLI+TDY MPGMTGYELLKKIKESSA +E+PVV+MSSENI TRI++CLEEGA+ F
Sbjct: 281 DGVKVNLIMTDYSMPGMTGYELLKKIKESSAFREIPVVVMSSENILTRIDRCLEEGAEDF 460
Query: 130 ILKPLKQSDIKKLK 143
+LKP+K SD+++L+
Sbjct: 461 LLKPVKLSDVRRLR 502
>AV415712
Length = 353
Score = 105 bits (263), Expect = 3e-24
Identities = 54/79 (68%), Positives = 65/79 (81%)
Frame = +2
Query: 20 HVLAVDDNLIDRKLVEKLLRNSSCKVTTAENGPRALEFLGLTSGEQNTLNGRSKVNLIIT 79
HVLAVDD+LIDRK++E+LL+ SSCKVT ++G RAL+FLGL E + KV+L+IT
Sbjct: 119 HVLAVDDSLIDRKVIERLLKISSCKVTAVDSGIRALQFLGL-EPESDDFAPCLKVDLVIT 295
Query: 80 DYCMPGMTGYELLKKIKES 98
DYCMPGMTGYELLKKIKES
Sbjct: 296 DYCMPGMTGYELLKKIKES 352
>TC12957 similar to UP|Q9FRY9 (Q9FRY9) Response regulator 6, partial (27%)
Length = 493
Score = 92.4 bits (228), Expect = 3e-20
Identities = 49/101 (48%), Positives = 65/101 (63%), Gaps = 16/101 (15%)
Frame = +2
Query: 20 HVLAVDDNLIDRKLVEKLLRNSSCK----------------VTTAENGPRALEFLGLTSG 63
HVLAVDD+LIDR L+E+LL+ SS VT ++ +AL FLGL
Sbjct: 191 HVLAVDDSLIDRMLIERLLKTSSFHGGILIADILGMCVEFTVTAVDSASKALNFLGLVED 370
Query: 64 EQNTLNGRSKVNLIITDYCMPGMTGYELLKKIKESSAMKEV 104
+ ++ VNLIITDYCMPGMTGY+LL+KIKES ++K++
Sbjct: 371 MASEIHQDVDVNLIITDYCMPGMTGYDLLRKIKESKSLKDI 493
>BP073830
Length = 528
Score = 62.0 bits (149), Expect = 5e-11
Identities = 28/45 (62%), Positives = 38/45 (84%)
Frame = -3
Query: 88 GYELLKKIKESSAMKEVPVVIMSSENIPTRINKCLEEGAQMFILK 132
GY+LLK+IKESS+++ +PVVI S EN+ +RINKCLEEG + F L+
Sbjct: 475 GYDLLKEIKESSSLRYIPVVIRSFENVLSRINKCLEEGVEEFFLE 341
>BP079485
Length = 473
Score = 51.6 bits (122), Expect = 6e-08
Identities = 34/74 (45%), Positives = 36/74 (47%), Gaps = 37/74 (50%)
Frame = +2
Query: 106 VVIMSSENIPTRINK-------------------------------------CLEEGAQM 128
VVIMSS+NIPT+INK CLEEGAQM
Sbjct: 29 VVIMSSKNIPTQINK*FN*FLVFPFSSFHPTIVSCTCILLNNVLNHFFALTRCLEEGAQM 208
Query: 129 FILKPLKQSDIKKL 142
FILKPLKQSD KKL
Sbjct: 209 FILKPLKQSDTKKL 250
Score = 30.4 bits (67), Expect = 0.15
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = +3
Query: 146 LSNCSSLGVGMHIIVF 161
LSN SSLGVGMHI VF
Sbjct: 411 LSNLSSLGVGMHITVF 458
>TC8860 similar to UP|Q9C5T8 (Q9C5T8) Cytokinin receptor CRE1b, partial
(13%)
Length = 1012
Score = 47.0 bits (110), Expect = 2e-06
Identities = 35/143 (24%), Positives = 62/143 (42%), Gaps = 18/143 (12%)
Frame = +2
Query: 21 VLAVDDNLIDRKLVEKLLRNSSCKVTTAENGPRALEFLGLTSGEQNTLNGRSKVNLIITD 80
+L VDDNL++R++ L+N V A +G ALE L + D
Sbjct: 77 ILVVDDNLVNRRVAAGALKNFGADVKCALSGKAALEMLQYP----------DDFDSCFMD 226
Query: 81 YCMPGMTGYELLKKIK---------------ESSAMK---EVPVVIMSSENIPTRINKCL 122
MP M G+E ++I+ E + K +P++ M+++ I +KCL
Sbjct: 227 IQMPEMDGFEATRRIRMMEREASEQLKSESGEENGKKSEFHMPILAMTADVIHATYDKCL 406
Query: 123 EEGAQMFILKPLKQSDIKKLKCK 145
G ++ KP ++ ++ + K
Sbjct: 407 NCGMDGYVSKPFEEENLYQAVAK 475
>TC13528 similar to UP|Q9SXL4 (Q9SXL4) Histidine kinase 1, partial (6%)
Length = 520
Score = 39.3 bits (90), Expect = 3e-04
Identities = 22/70 (31%), Positives = 38/70 (53%), Gaps = 2/70 (2%)
Frame = +3
Query: 75 NLIITDYCMPGMTGYELLKKIKESS--AMKEVPVVIMSSENIPTRINKCLEEGAQMFILK 132
+LI+ D MP M GYE K I++S +P+V +++ + +CL+ G ++ K
Sbjct: 24 DLILMDCQMPKMDGYEATKAIRKSEEGTALHIPIVALTAHAMSCDEAQCLQVGMDAYLTK 203
Query: 133 PLKQSDIKKL 142
P+ D KK+
Sbjct: 204 PI---DFKKM 224
>BP055416
Length = 500
Score = 37.7 bits (86), Expect = 0.001
Identities = 19/62 (30%), Positives = 34/62 (54%), Gaps = 2/62 (3%)
Frame = -2
Query: 75 NLIITDYCMPGMTGYELLKKIKESSAMK--EVPVVIMSSENIPTRINKCLEEGAQMFILK 132
+L++ D MP M GYE K+I++S +P+V +++ + KCL G ++ K
Sbjct: 391 DLVLMDCQMPKMDGYEATKEIRKSEVGTGLHIPIVALTAHAMSCDEAKCLGVGMDAYLTK 212
Query: 133 PL 134
P+
Sbjct: 211 PI 206
>TC18201 homologue to GB|AAB86804.1|2454184|ATU80186 pyruvate dehydrogenase
E1 beta subunit {Arabidopsis thaliana;} , partial (25%)
Length = 636
Score = 28.1 bits (61), Expect = 0.76
Identities = 22/77 (28%), Positives = 37/77 (47%), Gaps = 4/77 (5%)
Frame = +2
Query: 52 PRALEFLGLTSGEQNTLNGRSKVN--LIITDYCM-PGMTGYELLKKIKES-SAMKEVPVV 107
P ++ L + +T+ K ++I + CM G G L I E+ + + PVV
Sbjct: 26 PEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAITENFNDYLDAPVV 205
Query: 108 IMSSENIPTRINKCLEE 124
+SS+++PT LEE
Sbjct: 206 CLSSQDVPTPYTGPLEE 256
>TC8437 homologue to GB|AAB86804.1|2454184|ATU80186 pyruvate dehydrogenase
E1 beta subunit {Arabidopsis thaliana;} , partial (40%)
Length = 915
Score = 27.7 bits (60), Expect = 0.99
Identities = 23/86 (26%), Positives = 42/86 (48%), Gaps = 4/86 (4%)
Frame = +1
Query: 52 PRALEFLGLTSGEQNTLNGRSKVN--LIITDYCM-PGMTGYELLKKIKES-SAMKEVPVV 107
P ++ L + +T+ K ++I + CM G G L I E+ + + P+V
Sbjct: 205 PEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAISENFNDYLDAPIV 384
Query: 108 IMSSENIPTRINKCLEEGAQMFILKP 133
+SS+++PT LEE M +++P
Sbjct: 385 CLSSQDVPTPYAGPLEE---MTVVQP 453
>TC19121 similar to UP|ENAH_MOUSE (Q03173) Enabled protein homolog (NPC
derived proline-rich protein 1) (NDPP-1), partial (3%)
Length = 414
Score = 26.6 bits (57), Expect = 2.2
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -2
Query: 124 EGAQMFILKPLKQSDIKKLKCKLS 147
EGAQ +L P+K + ++L CKL+
Sbjct: 332 EGAQAALLDPMKTVEGRQLNCKLA 261
>TC18633 similar to GB|AAB94051.1|2736075|AF016644 PAP-specific phosphatase
{Arabidopsis thaliana;} , partial (45%)
Length = 882
Score = 26.2 bits (56), Expect = 2.9
Identities = 12/42 (28%), Positives = 21/42 (49%)
Frame = +3
Query: 112 ENIPTRINKCLEEGAQMFILKPLKQSDIKKLKCKLSNCSSLG 153
E + +N+CL E Q + KP ++ +S C+S+G
Sbjct: 330 EAVVRTVNECLAEAPQFGVPKPKSALGASQVLEIISRCNSIG 455
>BP044479
Length = 548
Score = 24.6 bits (52), Expect = 8.4
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 61 TSGEQNTLNGRSKVNLIITDYCMPGMT 87
TS QN +G+ ++NL+ +C P +T
Sbjct: 344 TSTLQNMTSGKQQINLLKRYHCRPFLT 424
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.132 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,669,307
Number of Sequences: 28460
Number of extensions: 29611
Number of successful extensions: 153
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of query: 161
length of database: 4,897,600
effective HSP length: 83
effective length of query: 78
effective length of database: 2,535,420
effective search space: 197762760
effective search space used: 197762760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0320b.4