BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0318.12
(1553 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP054462 278 4e-75
BP055633 247 1e-65
AV427422 196 2e-50
BP055130 138 1e-35
BP046863 131 9e-31
BF177840 93 4e-19
BG662087 92 6e-19
AU089582 74 1e-17
AV426985 75 8e-14
AV410603 74 2e-13
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 55 1e-07
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 47 2e-05
BP063723 44 3e-04
AV410570 40 0.002
AV777492 39 0.005
TC11563 39 0.008
TC18698 38 0.014
TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526 At2g01... 37 0.018
TC19663 similar to UP|Q8H6S8 (Q8H6S8) Translation initiation fac... 36 0.041
TC15001 similar to UP|GLE1_YEAST (Q12315) RNA export factor GLE1... 36 0.053
>BP054462
Length = 422
Score = 278 bits (712), Expect = 4e-75
Identities = 139/140 (99%), Positives = 139/140 (99%)
Frame = +1
Query: 1311 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 1370
NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 180
Query: 1371 MMRAYANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAY 1430
MMR YANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAY
Sbjct: 181 MMRTYANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAY 360
Query: 1431 RLELPAHSRVHPVFHVSLLK 1450
RLELPAHSRVHPVFHVSLLK
Sbjct: 361 RLELPAHSRVHPVFHVSLLK 420
>BP055633
Length = 528
Score = 247 bits (630), Expect = 1e-65
Identities = 126/127 (99%), Positives = 127/127 (99%)
Frame = -2
Query: 181 VTTWTKFKEAMLERFQLTSNSSPFAALLALKQEGSVEEFVGQFERFAGMLKGIDEEHYMD 240
VTTWTKFKEAMLE+FQLTSNSSPFAALLALKQEGSVEEFVGQFERFAGMLKGIDEEHYMD
Sbjct: 527 VTTWTKFKEAMLEQFQLTSNSSPFAALLALKQEGSVEEFVGQFERFAGMLKGIDEEHYMD 348
Query: 241 IFVNGLKEEIAAEIKLYEPKSLTIMVKKALMVEQKNLAVSKAGIGSTSRYNSSFKPPFRS 300
IFVNGLKEEIAAEIKLYEPKSLTIMVKKALMVEQKNLAVSKAGIGSTSRYNSSFKPPFRS
Sbjct: 347 IFVNGLKEEIAAEIKLYEPKSLTIMVKKALMVEQKNLAVSKAGIGSTSRYNSSFKPPFRS 168
Query: 301 TTFQKSV 307
TTFQKSV
Sbjct: 167 TTFQKSV 147
Score = 49.3 bits (116), Expect = 5e-06
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = -2
Query: 785 LIYSLTMEEHVVHLTQVLQLMDQHDLK 811
L+YSLTMEEHVVHLTQVL LMD DLK
Sbjct: 104 LVYSLTMEEHVVHLTQVLXLMDHXDLK 24
>AV427422
Length = 417
Score = 196 bits (499), Expect = 2e-50
Identities = 83/137 (60%), Positives = 112/137 (81%)
Frame = +1
Query: 1186 GLPKSNGKDTILVVVDRLTKYAHFIPLRHPFTAPEVAALFLHEVVRLHGFPTTIVSDRDS 1245
GLPKS G + +LVVVDRL+K++HF+PL+HP+TA +A +F+ EVVRLHG P +IVSDRD
Sbjct: 7 GLPKSKGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDP 186
Query: 1246 LFLSSFWKELFRVSGTQLKFSSAYHPQTDGQTEVVNRCLEVYLRCLTGSRPRKWVTCLPW 1305
LF+S+FWKELF++ GT+LK S+AYHP++DGQTEVVNRCLE YLRC +P+ W +PW
Sbjct: 187 LFMSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPW 366
Query: 1306 AEFWFNSNYNRSAKMSP 1322
AE+W+N++Y+ S +P
Sbjct: 367 AEYWYNTSYHVSTGQTP 417
>BP055130
Length = 567
Score = 138 bits (348), Expect(2) = 1e-35
Identities = 63/112 (56%), Positives = 79/112 (70%)
Frame = +2
Query: 1192 GKDTILVVVDRLTKYAHFIPLRHPFTAPEVAALFLHEVVRLHGFPTTIVSDRDSLFLSSF 1251
G I+VVVDRL+KY HF P R +T+ +VA F+ +V+LHG P IVSDRD F S+F
Sbjct: 179 GFTVIIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAF 358
Query: 1252 WKELFRVSGTQLKFSSAYHPQTDGQTEVVNRCLEVYLRCLTGSRPRKWVTCL 1303
WK F++ GT L SS+YHPQTDGQTE +N+CLE+YLRC PR WV+ L
Sbjct: 359 WKHFFKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLWVSYL 514
Score = 30.0 bits (66), Expect(2) = 1e-35
Identities = 10/17 (58%), Positives = 14/17 (81%)
Frame = +3
Query: 1301 TCLPWAEFWFNSNYNRS 1317
TCLPWAE+ +NS++ S
Sbjct: 510 TCLPWAEYCYNSSFQTS 560
>BP046863
Length = 580
Score = 131 bits (329), Expect = 9e-31
Identities = 65/112 (58%), Positives = 87/112 (77%)
Frame = +1
Query: 1339 TIPSKIAAVNDLQVGRDELLSDLRANLLKSQDMMRAYANKKRRDVDYQIGDEVFLKLQPY 1398
+I + AVN L RD LL +LR NLLK+QD MRA ANK RR VDYQ+G+ VFLKLQPY
Sbjct: 241 SIKLSVEAVNMLIEERDALLLELRGNLLKAQDQMRAQANKHRRYVDYQVGNWVFLKLQPY 420
Query: 1399 RRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAYRLELPAHSRVHPVFHVSLLK 1450
+ ++LA++ N+KLSPR+YGP+ ++ ++ VAY L+L + SRVHPVFH+SLL+
Sbjct: 421 KLQNLAQRKNQKLSPRFYGPFKVLERVVQVAY*LDLXSESRVHPVFHLSLLE 576
>BF177840
Length = 410
Score = 92.8 bits (229), Expect = 4e-19
Identities = 47/106 (44%), Positives = 65/106 (60%)
Frame = +2
Query: 1237 TTIVSDRDSLFLSSFWKELFRVSGTQLKFSSAYHPQTDGQTEVVNRCLEVYLRCLTGSRP 1296
T+IVSDRD+ F+S FW+ L+ GT+L +S+ HPQTDGQTEVVN+ L LR +
Sbjct: 11 TSIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNL 190
Query: 1297 RKWVTCLPWAEFWFNSNYNRSAKMSPFQALYGREPPVLLQGTTIPS 1342
+ W T LP EF +N + + K SPF+ +YG P L +P+
Sbjct: 191 KMWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDLLPMPN 328
>BG662087
Length = 373
Score = 92.0 bits (227), Expect = 6e-19
Identities = 46/119 (38%), Positives = 67/119 (55%)
Frame = +1
Query: 676 GSWRFCVDYRALNKITVPNKFPIPVIDELLDEIGPARVFSKIDLKSGYHQIMMKAEDVEK 735
G WR VDY LNK + +P+P ID+L+D + S +D SGYHQI M D +K
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195
Query: 736 TAFRTHEGHYEFLVMPFGLTNAPSTFQSLMNEVLRPVLRKCALVFFDDILIYSLTMEEH 794
TAF T +Y + +PFGL NA +T+Q LM+ V + + V+ D++++ S H
Sbjct: 196 TAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKSALRANH 372
>AU089582
Length = 383
Score = 74.3 bits (181), Expect(2) = 1e-17
Identities = 35/72 (48%), Positives = 48/72 (66%)
Frame = +2
Query: 1218 APEVAALFLHEVVRLHGFPTTIVSDRDSLFLSSFWKELFRVSGTQLKFSSAYHPQTDGQT 1277
A + A ++L E+V LHG P +I+SDR + F S FW+ GT+LK S+A+HPQTDGQ+
Sbjct: 11 ASQYAKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQS 190
Query: 1278 EVVNRCLEVYLR 1289
E + LE LR
Sbjct: 191 ERTIQILEDMLR 226
Score = 33.9 bits (76), Expect(2) = 1e-17
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +3
Query: 1299 WVTCLPWAEFWFNSNYNRSAKMSPFQALYGR 1329
W L EF +N++Y S M+PF+ALYGR
Sbjct: 255 WDQYLSLMEFAYNNSYRSSI*MAPFEALYGR 347
>AV426985
Length = 422
Score = 75.1 bits (183), Expect = 8e-14
Identities = 38/131 (29%), Positives = 75/131 (57%), Gaps = 1/131 (0%)
Frame = +1
Query: 353 EMKEKLRKGECFRCDEKFGPNHICKNKQFRVMLLRDDGEEEEIETLEPLTEEIEEA-MEL 411
EM+ + K C+ CDEKF NH C NK ++ L D+ + ++ + T +++ +E
Sbjct: 28 EMQLRREKNLCYWCDEKFSFNHKCPNKYLMMLQLTDENDSDDTSNQQVTTTISDDSPVED 207
Query: 412 KHLSLNSIQGISSRKSLKVWGKLKGTAIVVLVDCGATHNFISQSLAREMELQITETPAYT 471
H SLN+++G + +++ G++ ++ VLVD G++ F+ +A+ ++L I P +
Sbjct: 208 HHFSLNAMRGFTGVGTIRFTGQIGNISVQVLVDGGSSDCFLQPHIAKFLQLPIESKPNFK 387
Query: 472 VEVGDGHVVKS 482
V VG+G +++
Sbjct: 388 VLVGNGQKMET 420
>AV410603
Length = 162
Score = 73.9 bits (180), Expect = 2e-13
Identities = 32/53 (60%), Positives = 42/53 (78%)
Frame = +1
Query: 691 TVPNKFPIPVIDELLDEIGPARVFSKIDLKSGYHQIMMKAEDVEKTAFRTHEG 743
TV + FP+P +DELLDE+ ++ FSK+DL+SGYHQI++K ED KT FRTH G
Sbjct: 4 TVKDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 54.7 bits (130), Expect = 1e-07
Identities = 24/42 (57%), Positives = 34/42 (80%), Gaps = 1/42 (2%)
Frame = +3
Query: 1410 KLSPRYYGPYPIVAKIGAVAYRLELPAH-SRVHPVFHVSLLK 1450
KLSPR+ GP+ ++ ++G+V+YRL LP S VHPVFHVS+L+
Sbjct: 48 KLSPRFIGPFEVLERVGSVSYRLALPPDLSAVHPVFHVSMLR 173
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 47.4 bits (111), Expect = 2e-05
Identities = 24/71 (33%), Positives = 38/71 (52%)
Frame = +2
Query: 1260 GTQLKFSSAYHPQTDGQTEVVNRCLEVYLRCLTGSRPRKWVTCLPWAEFWFNSNYNRSAK 1319
G Q++FSS HPQT+GQTE N+ + ++ +W+ LP + +N+ S K
Sbjct: 62 GIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWIDELPIVLWSYNTMPQSSIK 241
Query: 1320 MSPFQALYGRE 1330
+PF YG +
Sbjct: 242 ETPF*LTYGAD 274
>BP063723
Length = 479
Score = 43.5 bits (101), Expect = 3e-04
Identities = 25/59 (42%), Positives = 33/59 (55%), Gaps = 4/59 (6%)
Frame = -3
Query: 1494 IQWHSLPACENSWESAAQLHEAFPDFPLEDKVKHLGGGID----EHRPSIKKVYERRNK 1548
I+W LP E+SWE +L + FP+ LED++ +L GG D RP VY RR K
Sbjct: 477 IRWKDLPTFEDSWEDFCKLLDPFPNHQLEDQL-NLQGGRDVANPSSRPRFGDVYARRPK 304
>AV410570
Length = 412
Score = 40.4 bits (93), Expect = 0.002
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +2
Query: 347 RKLTGEEMKEKLRKGECFRCDEKFGPNHICKNKQ 380
++++ EM+ + KG CF CDEK+ NH C NKQ
Sbjct: 83 KRISPAEMQLRREKGLCFTCDEKYSWNHKCPNKQ 184
>AV777492
Length = 472
Score = 39.3 bits (90), Expect = 0.005
Identities = 15/19 (78%), Positives = 18/19 (93%)
Frame = +1
Query: 1280 VNRCLEVYLRCLTGSRPRK 1298
+NRCLEVY RCLTG+RP+K
Sbjct: 403 LNRCLEVYFRCLTGARPKK 459
>TC11563
Length = 470
Score = 38.5 bits (88), Expect = 0.008
Identities = 23/62 (37%), Positives = 31/62 (49%)
Frame = +2
Query: 1491 EVLIQWHSLPACENSWESAAQLHEAFPDFPLEDKVKHLGGGIDEHRPSIKKVYERRNKRK 1550
++LIQW C +WE + + ++FP F L DKV G G IKKV +K
Sbjct: 14 QLLIQWEGAANC--TWELLSYIQDSFPQFALADKVTFYGEG----NVMIKKVVGATSKGH 175
Query: 1551 VT 1552
VT
Sbjct: 176 VT 181
>TC18698
Length = 808
Score = 37.7 bits (86), Expect = 0.014
Identities = 17/55 (30%), Positives = 33/55 (59%)
Frame = -2
Query: 734 EKTAFRTHEGHYEFLVMPFGLTNAPSTFQSLMNEVLRPVLRKCALVFFDDILIYS 788
+KT + + +Y + VMP GL N +T+Q LM+++ + K V+ +D+++ S
Sbjct: 804 KKTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKS 640
>TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526
At2g01100/F23H14.7 {Arabidopsis thaliana;}, partial
(33%)
Length = 902
Score = 37.4 bits (85), Expect = 0.018
Identities = 29/128 (22%), Positives = 54/128 (41%), Gaps = 7/128 (5%)
Frame = +3
Query: 3 ASRMEARVEAIERFIEVMNRDREEERRNREEDRRIRDEERRERAIEHERVEQRF------ 56
A++ +A EA ER ++ + + + E D DE +R H + ++R
Sbjct: 330 AAKAKADAEARERKLKAAKHKKRADS-DSESDYDSDDESKRTSRRSHRKHKKRSHYDSDH 506
Query: 57 -RNLEAIVATMTRRRAGEHGDSDGEQERRRMEEERTVNGGQPGSVRDEADARENLQRTAN 115
+ E + T++ + E D G++ R EEER Q +RD+ ++ ++
Sbjct: 507 EKRKEKLSKRKTKKWSSESSDFSGDESESRSEEERRRKRKQKKKLRDQVSRSDSSDSDSS 686
Query: 116 AQTERWRK 123
A RK
Sbjct: 687 ADEVSKRK 710
>TC19663 similar to UP|Q8H6S8 (Q8H6S8) Translation initiation factor,
partial (10%)
Length = 485
Score = 36.2 bits (82), Expect = 0.041
Identities = 23/73 (31%), Positives = 39/73 (52%), Gaps = 4/73 (5%)
Frame = +3
Query: 22 RDREEERRNREEDRRIRDEE---RRERAIEHERVEQRFRNLEAIVATMTRRR-AGEHGDS 77
R EER+ REE+ ++R EE RR+ +E + E R R E + +++ G+
Sbjct: 231 RKEAEERKKREEEEKLRKEEEERRRQEELERQAEEARRRKKEREKEKLLKKKQEGKLLTG 410
Query: 78 DGEQERRRMEEER 90
++E+RR+E R
Sbjct: 411 KQKEEQRRLEAMR 449
>TC15001 similar to UP|GLE1_YEAST (Q12315) RNA export factor GLE1, partial
(4%)
Length = 988
Score = 35.8 bits (81), Expect = 0.053
Identities = 29/91 (31%), Positives = 44/91 (47%), Gaps = 9/91 (9%)
Frame = +2
Query: 2 VASRMEARVEAIERFIEVMNRDREEERRNREED---------RRIRDEERRERAIEHERV 52
VA ++E EA IE R++EE+ R +ED ++I + +RRE + R
Sbjct: 518 VAVQLEKEKEAA--LIEA--REKEEQARKEKEDLDRMLEENRKKIEEAQRREALEQQRRE 685
Query: 53 EQRFRNLEAIVATMTRRRAGEHGDSDGEQER 83
E+R+R LE I + A + EQER
Sbjct: 686 EERYRELEEI--QRQKEEAMRRKKQEEEQER 772
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,586,914
Number of Sequences: 28460
Number of extensions: 332764
Number of successful extensions: 1808
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 1759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1799
length of query: 1553
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1451
effective length of database: 1,994,680
effective search space: 2894280680
effective search space used: 2894280680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0318.12