BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0318.1
(799 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16580 146 1e-35
BE122634 77 8e-15
BP053707 59 4e-09
BP053311 34 0.10
TC12439 similar to UP|Q9FMJ2 (Q9FMJ2) Ankyrin-like protein, part... 33 0.14
BU494198 33 0.14
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 33 0.18
TC15123 similar to UP|AAM51564 (AAM51564) Flavonoid 3', 5'-hydro... 31 0.88
AU089281 31 0.88
TC10820 weakly similar to UP|Q9M9Y0 (Q9M9Y0) F4H5.19 protein, pa... 30 1.1
TC15522 30 1.5
TC11703 homologue to UP|Q9ZSQ6 (Q9ZSQ6) T-complex protein 1 epsi... 30 2.0
TC14764 similar to UP|O22198 (O22198) 4-alpha-glucanotransferase... 29 3.3
TC10064 29 3.3
CB826695 28 4.4
TC8372 similar to UP|O04334 (O04334) At2g30610 protein, partial ... 28 4.4
TC10720 similar to GB|AAM70580.1|21700913|AY124871 At2g47330/T8I... 28 5.7
BP036242 28 7.4
TC7839 homologue to UP|Q93XW1 (Q93XW1) 14-3-3 protein, complete 28 7.4
AV767945 27 9.7
>TC16580
Length = 683
Score = 146 bits (368), Expect = 1e-35
Identities = 80/169 (47%), Positives = 109/169 (64%)
Frame = +2
Query: 629 SDLLMLPKDMLIDRHVREEVCPSITLSLIIRVLCNFTPDEFCPDPVPGTVLEALNAETIA 688
SDLLMLPKDML+ +R+EVCP S I ++L NF PDEFCPDP+P V EAL+++
Sbjct: 2 SDLLMLPKDMLLSESIRKEVCPMFNASQIKKILDNFVPDEFCPDPIPTDVFEALDSKDDL 181
Query: 689 ERRLSAESVRSFPYAAAAVVYVPPSSSNVAEKVAEAGGKSHLARNVSAVQRRGYTSDEEL 748
E +SV +FP AA +VY PP ++ +A + G +S L R+ S+V R+ YTSD+EL
Sbjct: 182 ED--GKDSVNNFPCIAAPIVYSPPPATTIASITGDIGSESQLRRSQSSVVRKSYTSDDEL 355
Query: 749 EELDSPLTSIIDKLPLSPTVSANGQDNQKEHKSYTTTTNARYQLLREVW 797
+EL+SPL+SI+ SP VS +KE + T + RY+LLR VW
Sbjct: 356 DELNSPLSSILFSGSPSP-VSTKPNWKKKESR---TESAVRYELLRNVW 490
>BE122634
Length = 301
Score = 77.4 bits (189), Expect = 8e-15
Identities = 38/72 (52%), Positives = 52/72 (71%)
Frame = +1
Query: 579 NSVGNWSRWLTDMFGMDVEDCVQEYQDSGENDERQGGDGEPKPFVLLNDLSDLLMLPKDM 638
+S+G+WSRWL+D+FG+D D ++DS END + + KPF LLN LSDL+MLP +M
Sbjct: 88 DSIGDWSRWLSDLFGIDDSD---SHEDSNENDGPKY-ESSFKPFPLLNSLSDLMMLPSEM 255
Query: 639 LIDRHVREEVCP 650
L D +R+EVCP
Sbjct: 256 LADGSMRKEVCP 291
>BP053707
Length = 476
Score = 58.5 bits (140), Expect = 4e-09
Identities = 41/104 (39%), Positives = 60/104 (57%), Gaps = 2/104 (1%)
Frame = -2
Query: 696 SVRSFPYAAAAVVYVPPSSSNVAEKVAEAGGKSHLARNVSAVQRRGYTSDEELEELDSPL 755
S+ SFP +A + Y PP + +V + E G + L R+ S V ++ YTSD+EL+ELDSPL
Sbjct: 457 SITSFPCSAGSTFYEPPPARSVVXMLKEVGTPNSL-RSGSFVLQKLYTSDDELDELDSPL 281
Query: 756 TSI-IDKLPL-SPTVSANGQDNQKEHKSYTTTTNARYQLLREVW 797
+++ +D L S A G+ +K RY+LLREVW
Sbjct: 280 SALGMDDSSLGSMKKFAVGKGGRKV---------VRYELLREVW 176
>BP053311
Length = 529
Score = 33.9 bits (76), Expect = 0.10
Identities = 31/140 (22%), Positives = 56/140 (39%), Gaps = 2/140 (1%)
Frame = +2
Query: 148 SKVKVSPSESSRGLKERSDRKTNKLQSKVSDSNQKKPMNSTKGPSRVTNKNTSSNNSKPV 207
S++ +S S+S G + RKT K + N +KP N T P N N+
Sbjct: 53 SQLGLSTSQSYSGFNDVDFRKTKPNTEKATPQNTQKPKNDTNRPHEHPNPNSKPKXKXRP 232
Query: 208 KA--PVKASSESSEGVDENHVLEVKEIEILDGASNGAQSLGSEDERHETVNAEENGEHED 265
+ PV + ++++ N+ + ++ ++ + G E V E E
Sbjct: 233 RXXPPVVSVDDANKDKGFNNFKSLPKLPLMKASVXGVWF-------EEAVELEAKVIGEG 391
Query: 266 KAALELKIEEMELRVEKLEE 285
K A + E + V+K +E
Sbjct: 392 KKAEIRNVGEWKSLVQKKKE 451
>TC12439 similar to UP|Q9FMJ2 (Q9FMJ2) Ankyrin-like protein, partial (5%)
Length = 534
Score = 33.5 bits (75), Expect = 0.14
Identities = 24/87 (27%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = +3
Query: 134 NEDEKVEKASKDAKSKVKVSP-SESSRGLKERSDRKTNKLQSKVSDSNQKKPMNSTKGPS 192
N D + + D K + +V SES +KE+ T+ + D P ++TKG +
Sbjct: 258 NVDVSQDSTTNDVKEQSEVKQQSESESEVKEQPTDTTDSKPRRFEDDAGDLPEDATKGDT 437
Query: 193 RVTNKNTSSNNSKPVKAPVKASSESSE 219
VT++N N+ P K K + +E
Sbjct: 438 SVTSEN---NSDLPEKQEDKLDEKPAE 509
>BU494198
Length = 510
Score = 33.5 bits (75), Expect = 0.14
Identities = 26/115 (22%), Positives = 48/115 (41%)
Frame = +3
Query: 134 NEDEKVEKASKDAKSKVKVSPSESSRGLKERSDRKTNKLQSKVSDSNQKKPMNSTKGPSR 193
N D SKD S K + S+ +S ++T+K+ + + K ++T GP +
Sbjct: 33 NTDSSKISRSKDDVSTPKSAKSKHETPKTGKSKQETSKIAAASKAKSPKSGKSNTNGPGK 212
Query: 194 VTNKNTSSNNSKPVKAPVKASSESSEGVDENHVLEVKEIEILDGASNGAQSLGSE 248
V + S +S++ DE +E+E G ++ + +GSE
Sbjct: 213 VKS----------------VSLKSTDSEDETSDDSTREVEDTKGKTSTSSKVGSE 329
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 33.1 bits (74), Expect = 0.18
Identities = 24/86 (27%), Positives = 38/86 (43%), Gaps = 2/86 (2%)
Frame = -2
Query: 182 KKPMNSTKGPSRVTNKNTSSNNSK-PVKAPVKASSESSEGVDE-NHVLEVKEIEILDGAS 239
++ ++S G N N SN+ K P P + G DE + E +E + D
Sbjct: 551 EEDLSSEDGAGYGNNSNNKSNSKKAPEGGPGAGAGPEENGEDEEDEDGEDQEDDDDDDED 372
Query: 240 NGAQSLGSEDERHETVNAEENGEHED 265
+ + G EDE E V E+N + E+
Sbjct: 371 DDEEDDGGEDEEEEGVEEEDNEDEEE 294
>TC15123 similar to UP|AAM51564 (AAM51564) Flavonoid 3', 5'-hydroxylase,
partial (24%)
Length = 592
Score = 30.8 bits (68), Expect = 0.88
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = -2
Query: 2 KETEKRKASRNSQTKGS--RRTERREDKLHQDNSSKTLNEKGTESKTPQD 49
K TE+ ++ +SQ K S RTE + H D SS++ + ++ TP+D
Sbjct: 219 KCTEQAQSPSSSQHKSSSQHRTESARNHSHADQSSRSSRSETSQDYTPED 70
>AU089281
Length = 376
Score = 30.8 bits (68), Expect = 0.88
Identities = 24/73 (32%), Positives = 36/73 (48%), Gaps = 1/73 (1%)
Frame = -2
Query: 163 ERSDRKTNKLQSKVSDSNQ-KKPMNSTKGPSRVTNKNTSSNNSKPVKAPVKASSESSEGV 221
E+S R +K ++K S+ Q K S K PS+ + S NSK A A+ S
Sbjct: 357 EKSRRFISKTEAKKSNEQQ*TKADKSRKKPSKQRRR---SRNSKAEAAMHIAAETSRNPT 187
Query: 222 DENHVLEVKEIEI 234
D N ++ KEI++
Sbjct: 186 DNNKLMAEKEIQL 148
>TC10820 weakly similar to UP|Q9M9Y0 (Q9M9Y0) F4H5.19 protein, partial (7%)
Length = 619
Score = 30.4 bits (67), Expect = 1.1
Identities = 19/43 (44%), Positives = 22/43 (50%), Gaps = 6/43 (13%)
Frame = +2
Query: 1 MKETEKRKASRNSQTKGS------RRTERREDKLHQDNSSKTL 37
+KE EKRKA + K RR ERRE QD SSK +
Sbjct: 140 LKENEKRKAHEAEKAKEEQLSKKRRREERREKYRTQDKSSKKM 268
>TC15522
Length = 1383
Score = 30.0 bits (66), Expect = 1.5
Identities = 23/89 (25%), Positives = 39/89 (42%), Gaps = 3/89 (3%)
Frame = +1
Query: 146 AKSKVKVSPSESSRGLKERSDRKTNKLQSKVSDSNQKKPMNSTKGPSRVTNKNTSSNNSK 205
AKS SPS +S S K+ ++ + P + ++ N S++S
Sbjct: 313 AKSLDFASPSSASPSSSVFSPMKSYFSSPCLNSFTMQAPNPESLALNKDVNSKKGSSSSS 492
Query: 206 PVKAPVKASSESSEGV---DENHVLEVKE 231
P+K S E+S+ V D NH+ ++ E
Sbjct: 493 PMKCDAPRSEETSDSVTTEDLNHLCQLVE 579
>TC11703 homologue to UP|Q9ZSQ6 (Q9ZSQ6) T-complex protein 1 epsilon subunit
(Fragment), partial (53%)
Length = 665
Score = 29.6 bits (65), Expect = 2.0
Identities = 24/83 (28%), Positives = 40/83 (47%), Gaps = 7/83 (8%)
Frame = +1
Query: 81 VNRSEEALAEMKVNAMVA-------NQASDTEKEQKEGNGEVSDTDTVKDSVSSQGDSFT 133
VNR + +LAE+ VNA+VA + D K + + G++ DT+ + V + S
Sbjct: 4 VNRCKRSLAEIAVNAVVAVADLERKDVNLDLIKVEGKVGGKLEDTELIYGIVVDKDMSHP 183
Query: 134 NEDEKVEKASKDAKSKVKVSPSE 156
+++E DAK + P E
Sbjct: 184 QMPKQIE----DAKIAILTCPFE 240
>TC14764 similar to UP|O22198 (O22198) 4-alpha-glucanotransferase, partial
(10%)
Length = 608
Score = 28.9 bits (63), Expect = 3.3
Identities = 20/54 (37%), Positives = 28/54 (51%), Gaps = 5/54 (9%)
Frame = +3
Query: 696 SVRSFPYAAAAVVYVPPSSSNVAE-----KVAEAGGKSHLARNVSAVQRRGYTS 744
S RSFP+ + V P S+S+VAE K+A A K L + VQ R + +
Sbjct: 201 SGRSFPHEDSQVEASPVSASSVAEAVSDKKIAGAADKIRLPSESNGVQPRDHVA 362
>TC10064
Length = 593
Score = 28.9 bits (63), Expect = 3.3
Identities = 24/80 (30%), Positives = 37/80 (46%)
Frame = -2
Query: 292 ALEVSLYSVVPEHGSSAHKVHTPARRLSRLYLHASKHWTQNRRATIAKNTVSGLILVAKS 351
AL L+ + G H + P+ L +S QN AT K T+ LIL A+
Sbjct: 319 ALIWMLHHCI*NSGKKPHALDLPS-----FTLDSSNIGEQNCHATFFK-TLLQLILKAEP 158
Query: 352 CGNDVSRLTFWLSNTIVLRE 371
+ S +FW+S I+++E
Sbjct: 157 IMGEASVSSFWVSELILMKE 98
>CB826695
Length = 554
Score = 28.5 bits (62), Expect = 4.4
Identities = 23/80 (28%), Positives = 33/80 (40%), Gaps = 1/80 (1%)
Frame = +2
Query: 597 EDCVQE-YQDSGENDERQGGDGEPKPFVLLNDLSDLLMLPKDMLIDRHVREEVCPSITLS 655
E+ +Q Y D D+R EPK FV L L ++ +L + D H E P +
Sbjct: 50 EEVIQAWYMDDSNEDQRLPHHREPKEFVSLAKLDEIGVLSWKLDADNH---ETDPDLKKI 220
Query: 656 LIIRVLCNFTPDEFCPDPVP 675
R E CP+ +P
Sbjct: 221 CEERGYTYKDVCEICPEKMP 280
>TC8372 similar to UP|O04334 (O04334) At2g30610 protein, partial (98%)
Length = 1366
Score = 28.5 bits (62), Expect = 4.4
Identities = 19/44 (43%), Positives = 25/44 (56%)
Frame = +1
Query: 711 PPSSSNVAEKVAEAGGKSHLARNVSAVQRRGYTSDEELEELDSP 754
P SS+ V AEAG L+ ++ G+T EELEEL+SP
Sbjct: 898 PRSSAYVQLLYAEAGWFQGLSE---MLEYSGFTGWEELEELESP 1020
>TC10720 similar to GB|AAM70580.1|21700913|AY124871 At2g47330/T8I13.17
{Arabidopsis thaliana;}, partial (6%)
Length = 634
Score = 28.1 bits (61), Expect = 5.7
Identities = 15/37 (40%), Positives = 20/37 (53%)
Frame = +2
Query: 170 NKLQSKVSDSNQKKPMNSTKGPSRVTNKNTSSNNSKP 206
N+ Q S S + +NST S V TS+N+SKP
Sbjct: 188 NRRQFVASTSPAQSAVNSTSQSSGVNPSQTSTNSSKP 298
>BP036242
Length = 525
Score = 27.7 bits (60), Expect = 7.4
Identities = 26/88 (29%), Positives = 35/88 (39%)
Frame = +3
Query: 29 HQDNSSKTLNEKGTESKTPQDRSAANNLVGDSNTASENSETYENVVIDYVDDVNRSEEAL 88
H + L EK ES + R T N +TYE V+ D V + SEE
Sbjct: 3 HTPQPQQQLQEKSEESLSVSTRKKV--------TFDSNVKTYEPVLQDEVGGLRESEEG- 155
Query: 89 AEMKVNAMVANQASDTEKEQKEGNGEVS 116
KV A+V + S T+ E + S
Sbjct: 156 --GKVEALV--KQSQTKSSSSEDSSVTS 227
>TC7839 homologue to UP|Q93XW1 (Q93XW1) 14-3-3 protein, complete
Length = 1284
Score = 27.7 bits (60), Expect = 7.4
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 9/47 (19%)
Frame = -2
Query: 268 ALELKIEEM---------ELRVEKLEEELREVAALEVSLYSVVPEHG 305
+L LK+E + E RV+KLE+ + +V L + L SVV + G
Sbjct: 500 SLHLKVEHLGITGGSRGDESRVKKLEDTVADVGELGLDLRSVVADDG 360
>AV767945
Length = 290
Score = 27.3 bits (59), Expect = 9.7
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 698 RSFPYAAAAVVYVPPSSSNVAEKVA 722
RSFP+ AA+ + SSS +EK+A
Sbjct: 196 RSFPFPAASFMLFTSSSSRTSEKIA 270
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.127 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,673,118
Number of Sequences: 28460
Number of extensions: 151063
Number of successful extensions: 758
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 750
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 753
length of query: 799
length of database: 4,897,600
effective HSP length: 98
effective length of query: 701
effective length of database: 2,108,520
effective search space: 1478072520
effective search space used: 1478072520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0318.1