BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.20
(396 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8398 weakly similar to UP|Q84KK1 (Q84KK1) S haplotype-specific... 78 9e-25
TC18972 106 6e-24
TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromo... 92 1e-19
BI419994 84 5e-17
BG662160 71 3e-13
AV418469 67 4e-12
AV768207 67 5e-12
AW720409 67 5e-12
AV423520 66 1e-11
AV427033 65 2e-11
TC19187 62 2e-10
BP076687 62 2e-10
BP052653 61 3e-10
AV413010 58 3e-09
TC18730 similar to UP|Q963J2 (Q963J2) Voltage-dependent calcium ... 57 7e-09
TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (... 56 9e-09
TC9055 54 5e-08
AI967453 54 6e-08
CB826914 53 1e-07
TC14801 50 3e-07
>TC8398 weakly similar to UP|Q84KK1 (Q84KK1) S haplotype-specific F-box
protein a, partial (9%)
Length = 1602
Score = 78.2 bits (191), Expect(2) = 9e-25
Identities = 67/211 (31%), Positives = 103/211 (48%), Gaps = 18/211 (8%)
Frame = +3
Query: 9 PWLPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYR-TSFSSVTSMTHQRL 67
P L DELI+EIL +PV S++ F+CVCKSWK IS+PQF K H R +S + ++
Sbjct: 72 PILIDELIWEILSLLPVKSLVRFRCVCKSWKLTISNPQFMKLHLRRSSAKAAADFADSQV 251
Query: 68 ASLGCYDRTPFSSVTSLTFQQLVSLAFYDDRK----SLTSCYVQSLLENPLAP-LETCRF 122
+ D S + S F + F D +L + Q + AP L + +
Sbjct: 252 VVMTKRDHIAHSPLLS-KFHIVHDETFTADEPWPVHALREPFNQPHVSICYAPSLFSSQN 428
Query: 123 NVQTKW-VHILGSCNGLLCLADR-----------SCLFRLMNPFTRLASKRSPPLDVRSP 170
N + K ++G+CNGL+ + + SC +NP T+L S+ SP L +
Sbjct: 429 NDKLKGHCRLIGACNGLVSVINEGYNSAESATKLSCCN--LNPATKLRSQVSPTLSLCYK 602
Query: 171 PVDLDVRDDVITQYGFGYDQVNNKYKVLITY 201
+ V +GFGYD +N+ YKV++ +
Sbjct: 603 QFHSLIHS-VFRIFGFGYDPLNDTYKVVVGF 692
Score = 52.0 bits (123), Expect(2) = 9e-25
Identities = 50/201 (24%), Positives = 86/201 (41%), Gaps = 11/201 (5%)
Frame = +1
Query: 206 VTKLYTFGTKSWTTIQNFPCDHQVPWRWW---DKQGKFVSGSLNWWVPERN---GIISFD 259
V +Y G W IQ+FP + W D+ G +++G++NW V N IIS D
Sbjct: 730 VANVYNMGDGCWRRIQSFPDFTREHWGRGTAPDQNGVYLNGTINW-VLRLNIAYDIISLD 906
Query: 260 LEKETYGEVLLPLHMGGD----HPFNHVLEVLSNRLCLCYFN-DIHCVVWLIKKYGNQQS 314
L E + LP + +L VL + LC+ + N D V+W + ++G +S
Sbjct: 907 LGSEVCSRLSLPCFPPPGPKCVYDLPPILGVLKDSLCVLHNNNDKTFVIWKMNEFGAHES 1086
Query: 315 WKKLMLIPHSKLNMAMPMPIHASCCLRPLCISENGIVFMRTIDLQLVSYNTKNGSSDDPE 374
W L K ++ SEN V + T+ Q++ ++ +
Sbjct: 1087WTPLFNFDFKKEDLYR---------FDKFFFSENDEVLLMTLTKQVLYNHSDITFKFNDR 1239
Query: 375 INMGRYRRDDMHIYHESLISP 395
++ + +++ Y ESL+SP
Sbjct: 1240VHNYIHDWNNIENYVESLVSP 1302
>TC18972
Length = 391
Score = 106 bits (265), Expect = 6e-24
Identities = 58/111 (52%), Positives = 68/111 (61%), Gaps = 6/111 (5%)
Frame = +2
Query: 291 LCLCYFNDIHCVVWLIKKYGNQQSWKKLMLIPHSKLNMAMPMPIHASCCLRPLCISENGI 350
LCLCY ND+HCV+W I YG Q+SW KL+ IP K+ PI A +RPLCISENG+
Sbjct: 2 LCLCYLNDMHCVLWSINMYGIQESWSKLISIPRCKIX----GPIGAFLHIRPLCISENGL 169
Query: 351 VFMRT----IDLQLVSYNTKNGSSDDPEINM--GRYRRDDMHIYHESLISP 395
V M LQLV YN NG D PEI R+ R M+IYHES +SP
Sbjct: 170 VLMEVDSDHFKLQLVMYNAVNGKLDYPEIMSPGNRHYRHYMYIYHESSVSP 322
>TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromosome 5, TAC
clone:K14B20, partial (7%)
Length = 782
Score = 92.4 bits (228), Expect = 1e-19
Identities = 88/273 (32%), Positives = 120/273 (43%), Gaps = 14/273 (5%)
Frame = +3
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDH--YRTSFSSVTSMTHQRLA 68
LPDELI EIL +PV S++ F+ V K+WK+ ISDPQF K H +R SF +
Sbjct: 48 LPDELIIEILSWLPVKSLLQFRVVSKTWKSFISDPQFVKLHLLHRLSFRNAD-------- 203
Query: 69 SLGCYDRTPFSSVTSLTFQQLVSLAFYDD--RKSLTSCYVQSLLENPLAPLETCRFNVQT 126
++ T L+ DD R ++S V SLLE+P A + +
Sbjct: 204 ----FEHT-----------SLLIKCHTDDFGRPYISSRTVSSLLESPSAIVAS---RSCI 329
Query: 127 KWVHILGSCNGLLCL---------ADRSCLFRLMNPFTRLASKRSPPLDVRSPPVDLDVR 177
+G+CNGL+ L + R NP TR S+ SPP P +L +
Sbjct: 330 SGYDFIGTCNGLVSLRKLNYDESNTNNFSQVRFWNPATRTMSQDSPP---SWSPRNLHL- 497
Query: 178 DDVITQYGFGYDQVNNKYKVLITYGFPRVTKLYTFGTKSWTTIQNFPCDHQVPWRWWDKQ 237
GFGYD ++ YKV+ + +Y G W TIQ P P
Sbjct: 498 -------GFGYDCSSDTYKVVGMIPGLTMVNVYNMGDNCWRTIQISP---HAPMH-LQGS 644
Query: 238 GKFVSGSLNWWV-PERNGIISFDLEKETYGEVL 269
+VS +LNW I+SFDLEKE + L
Sbjct: 645 AVYVSNTLNWLAGTNPYFIVSFDLEKEECAQQL 743
>BI419994
Length = 549
Score = 83.6 bits (205), Expect = 5e-17
Identities = 64/190 (33%), Positives = 87/190 (45%), Gaps = 2/190 (1%)
Frame = +2
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFSSVTSMTHQRLASL 70
LPDELI +IL R+PV S++ FK VCKSW+ LISDPQF K H F+ S TH
Sbjct: 47 LPDELILQILLRLPVRSLLRFKTVCKSWRYLISDPQFTKSH----FNLAASPTH------ 196
Query: 71 GCYDRTPFSSVTSLTFQQLVSLAFYDDRKSLTSCYVQSLLENPLAP--LETCRFNVQTKW 128
R ++ + L + + D SL L+ PLAP + RF
Sbjct: 197 ----RIFLNNTNGF*IESLDTDSSLHDESSLVH------LKFPLAPPHPDNHRFGSSVTG 346
Query: 129 VHILGSCNGLLCLADRSCLFRLMNPFTRLASKRSPPLDVRSPPVDLDVRDDVITQYGFGY 188
+ +LGSC G + LA+ + NP T + R P + YG GY
Sbjct: 347 LKVLGSCRGFVLLANHQGNVVVWNPSTGVRR--------RVPEWCSYLASQCEFLYGIGY 502
Query: 189 DQVNNKYKVL 198
D+ N+ Y V+
Sbjct: 503 DESNDDYLVV 532
>BG662160
Length = 445
Score = 71.2 bits (173), Expect = 3e-13
Identities = 60/192 (31%), Positives = 85/192 (44%), Gaps = 8/192 (4%)
Frame = +2
Query: 6 SRLPWLPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFSSVTSMTHQ 65
+R +LP+EL EIL +PV S++ F+CV KSWK++ISD QF K H S S+ +
Sbjct: 8 ARAQFLPEELRLEILSWLPVKSLVRFRCVSKSWKSIISDSQFIKLHLHRSSSTTRN---- 175
Query: 66 RLASLGCYDRTPFSSVTSLTFQQLVSLAFYDDRKSLTSCYVQSLLENPLAPLETCRFNVQ 125
T F+ Y+QSL+ +P +
Sbjct: 176 ----------TDFA-------------------------YLQSLITSPRKSRSASTIAIP 250
Query: 126 TKWVHILGSCNGLLCL----ADRSCLF----RLMNPFTRLASKRSPPLDVRSPPVDLDVR 177
+ G+CNGL+CL DR + RL NP R S+RSPPL + P+ D
Sbjct: 251 DDY-DFCGTCNGLVCLHSSKYDRKDKYTSHVRLWNPAMRSMSQRSPPLYL---PIVYD-- 412
Query: 178 DDVITQYGFGYD 189
+ +GF YD
Sbjct: 413 ----SYFGFDYD 436
>AV418469
Length = 316
Score = 67.4 bits (163), Expect = 4e-12
Identities = 45/116 (38%), Positives = 59/116 (50%)
Frame = +1
Query: 45 PQFAKDHYRTSFSSVTSMTHQRLASLGCYDRTPFSSVTSLTFQQLVSLAFYDDRKSLTSC 104
PQF KDH TS +S T MTH RLA LVS +D + +
Sbjct: 22 PQFGKDHLHTS-NSGTIMTHPRLA--------------------LVS----EDSSKVVTY 126
Query: 105 YVQSLLENPLAPLETCRFNVQTKWVHILGSCNGLLCLADRSCLFRLMNPFTRLASK 160
+ S +NP E +F ++ ++ H +GS NGLLCL D + FRL NPFTRL S+
Sbjct: 127 SMNSFFKNPPTLNEKRKFKMEGEYNHFVGSINGLLCLCDVNDCFRLWNPFTRLVSR 294
>AV768207
Length = 374
Score = 67.0 bits (162), Expect = 5e-12
Identities = 31/52 (59%), Positives = 39/52 (74%)
Frame = +1
Query: 9 PWLPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFSSVT 60
P LP EL EIL R+PV S++ +CVCKSWK+LISDP+FAK+H R S + T
Sbjct: 67 PTLPFELGVEILCRLPVKSLLQLRCVCKSWKSLISDPKFAKNHLRCSSTDFT 222
>AW720409
Length = 575
Score = 67.0 bits (162), Expect = 5e-12
Identities = 56/160 (35%), Positives = 75/160 (46%), Gaps = 4/160 (2%)
Frame = +3
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLI-SDPQFAKDHYRTSFSSVTSMTHQRLAS 69
LP EL EIL +PV ++M F CV KSWK+LI D F K H S +Q + +
Sbjct: 93 LPFELWIEILSWLPVKTLMRFSCVSKSWKSLIYQDRDFKKLHLDRS----PKNNNQVILT 260
Query: 70 LGCYDRTPFSSVTSLTFQQLVSLAFYDDRKSLTSCYVQSLLENPLAPLETCRFNVQTKWV 129
L + FS T F L D++ +S + + P E + + +
Sbjct: 261 L---QKPLFSGRTFFPFPVRRLL---QDQEPSSSSIIDDI------PFEEEDEDEEDNYH 404
Query: 130 HILGSCNGLLCL---ADRSCLFRLMNPFTRLASKRSPPLD 166
+GSCNGL+CL D FRL NP TR +SPPLD
Sbjct: 405 DTIGSCNGLVCLYGINDHGLWFRLWNPATRFRFHKSPPLD 524
>AV423520
Length = 468
Score = 65.9 bits (159), Expect = 1e-11
Identities = 43/112 (38%), Positives = 56/112 (49%), Gaps = 2/112 (1%)
Frame = +3
Query: 4 PHSRLPWLPDELIFEILFRVPVSSVMSFKCVCKSWKTLIS-DPQFAKDHYRTSFSSVTSM 62
P LP LP EL+ EIL R+PV S++ F+CVCKSW +LIS DP+FA+ H R S T
Sbjct: 144 PPPPLPTLPFELVVEILCRLPVKSLLQFRCVCKSWNSLISGDPKFARKHLRCSPKDFT-- 317
Query: 63 THQRLASLGCYDRTPFSSVTSLTFQQLVSLAFYDDRKSLT-SCYVQSLLENP 113
H + F S F L+ +DD ++ S L NP
Sbjct: 318 RHHLI----------FGSTNEFLFTSSPLLSVFDDAAAIAPSTQFDHPLNNP 443
>AV427033
Length = 420
Score = 65.5 bits (158), Expect = 2e-11
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +1
Query: 9 PWLPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTS 55
P LP +L+ EIL R+PV+S++ F+CVCKSW +LISDP+FAK+H S
Sbjct: 118 PPLPFDLVVEILCRLPVNSLLQFRCVCKSWNSLISDPKFAKNHLHCS 258
>TC19187
Length = 632
Score = 61.6 bits (148), Expect = 2e-10
Identities = 39/92 (42%), Positives = 52/92 (56%), Gaps = 7/92 (7%)
Frame = +1
Query: 238 GKFVSGSLNWWVPERNG------IISFDLEKETYGEVLLPLHMGGDHPFNHVLEVLSNRL 291
GKFVSG+LNW G I+S DL+KETY E+L P + + L VL L
Sbjct: 7 GKFVSGTLNWAANYSVGVSSLWIIVSLDLQKETYKEILPPDYEK-EECSTPTLSVLKGCL 183
Query: 292 CLCYFND-IHCVVWLIKKYGNQQSWKKLMLIP 322
C+ Y + VVW++K YG ++SW KL+ IP
Sbjct: 184 CMNYDHKRTDFVVWMMKDYGVRESWVKLVTIP 279
>BP076687
Length = 389
Score = 61.6 bits (148), Expect = 2e-10
Identities = 27/42 (64%), Positives = 34/42 (80%)
Frame = -3
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHY 52
LP+ELI +IL R+PV S++ FK VCKSW +LISDP+F K HY
Sbjct: 381 LPEELIPQILLRLPVRSLLRFKSVCKSWLSLISDPKFGKSHY 256
>BP052653
Length = 552
Score = 61.2 bits (147), Expect = 3e-10
Identities = 30/60 (50%), Positives = 39/60 (65%)
Frame = +1
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFSSVTSMTHQRLASL 70
LP +LI +IL R+PV S++ FK VCKSW +LISDPQF K H F + TH+ L +
Sbjct: 187 LPHDLIVQILLRLPVRSLLRFKSVCKSWLSLISDPQFGKSH----FDLAAAPTHRCLVKI 354
>AV413010
Length = 416
Score = 57.8 bits (138), Expect = 3e-09
Identities = 26/47 (55%), Positives = 36/47 (76%)
Frame = +3
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFS 57
LPDELI +IL ++PV+S++ FK V KSW + IS+PQFAK H+ + S
Sbjct: 96 LPDELIIQILLQLPVTSLLRFKSVSKSWFSQISNPQFAKSHFNLAAS 236
>TC18730 similar to UP|Q963J2 (Q963J2) Voltage-dependent calcium channel
alpha13 subunit (Fragment), partial (5%)
Length = 515
Score = 56.6 bits (135), Expect = 7e-09
Identities = 30/57 (52%), Positives = 36/57 (62%), Gaps = 4/57 (7%)
Frame = +2
Query: 5 HSRLP-WLP---DELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFS 57
H R P + P ELI IL R+PV S++ FK VCKSW +LISDP+F HY S S
Sbjct: 191 HGRTPLYFPISLKELILHILLRLPVRSLLRFKSVCKSWLSLISDPKFGXSHYDISAS 361
>TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (SLF)-S4
protein, partial (10%)
Length = 551
Score = 56.2 bits (134), Expect = 9e-09
Identities = 28/48 (58%), Positives = 35/48 (72%), Gaps = 2/48 (4%)
Frame = +3
Query: 11 LPDELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDH--YRTSF 56
LPDELI EIL +PV S++ F+ V K+WK+ ISDPQF K H +R SF
Sbjct: 21 LPDELIIEILSWLPVKSLLQFRVVSKTWKSFISDPQFVKLHLLHRLSF 164
Score = 45.1 bits (105), Expect = 2e-05
Identities = 39/115 (33%), Positives = 50/115 (42%), Gaps = 1/115 (0%)
Frame = +3
Query: 149 RLMNPFTRLASKRSPPLDVRSPPVDLDVRDDVITQYGFGYDQVNNKYKVLITYGFPRVTK 208
R NP TR S+ SPP P +L + GFGYD ++ YKV+ +
Sbjct: 252 RFWNPATRTMSQDSPP---SWSPRNLHL--------GFGYDCSSDTYKVVGMIPGLTMVN 398
Query: 209 LYTFGTKSWTTIQNFPCDHQVPWRWWDKQGKFVSGSLNWWV-PERNGIISFDLEK 262
+Y G W TIQ P P +VS +LNW I+SFDLEK
Sbjct: 399 VYNMGDNCWRTIQISP---HAPMH-LQGSAVYVSNTLNWLAGTNPYFIVSFDLEK 551
>TC9055
Length = 577
Score = 53.9 bits (128), Expect = 5e-08
Identities = 48/160 (30%), Positives = 73/160 (45%), Gaps = 7/160 (4%)
Frame = +2
Query: 243 GSLNWWVP-----ERNGIISFDLEKETYGEVLLPLHMGGDHPFNHVLEVLSNRLCLCY-F 296
G +NW E+ I+S D+ +E Y LL L G + ++VL N LCL Y F
Sbjct: 2 GCVNWLANKFEPFEQLVIVSLDMRQEAYR--LLSLPQGTSELPSAEIQVLGNCLCLFYDF 175
Query: 297 NDIHCVVWLIKKYGNQQSWKKLMLIPHSKLNMAMPMPIHASCCLRPLCISENGIVFMRTI 356
H V W + +YG +SW L+ I + L ++ +C+ +G +FM
Sbjct: 176 KRTHFVAWKMSEYGLPESWTPLLTISYEHLRWDHRFYLNQWL----ICLCGDGHIFMLAK 343
Query: 357 DLQLV-SYNTKNGSSDDPEINMGRYRRDDMHIYHESLISP 395
+LV YN + S E+ + D + Y ESL+SP
Sbjct: 344 KQKLVIIYNLSDNSVKYVELASNKLWL-DANDYVESLVSP 460
>AI967453
Length = 456
Score = 53.5 bits (127), Expect = 6e-08
Identities = 23/38 (60%), Positives = 29/38 (75%)
Frame = +1
Query: 15 LIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHY 52
LI EIL R+PV S++ FK VCK W++LISDP FA H+
Sbjct: 16 LITEILLRLPVKSLVRFKAVCKFWRSLISDPHFATSHF 129
>CB826914
Length = 575
Score = 52.8 bits (125), Expect = 1e-07
Identities = 23/46 (50%), Positives = 33/46 (71%)
Frame = +1
Query: 14 ELIFEILFRVPVSSVMSFKCVCKSWKTLISDPQFAKDHYRTSFSSV 59
+LI EIL +PV S++ FK VCK W++LISDP FA H++ + S+
Sbjct: 175 DLIIEILLMLPVKSIVRFKAVCKLWRSLISDPLFASLHFQRAAPSL 312
>TC14801
Length = 595
Score = 50.1 bits (118), Expect(2) = 3e-07
Identities = 44/127 (34%), Positives = 67/127 (52%), Gaps = 8/127 (6%)
Frame = +2
Query: 277 DHPFNHVLEVLSNRLCLC-YFNDIHC-VVWLIKKYGNQQSWKKLM-LIPHSKLNMAMPMP 333
DH F +L VL LC+ Y + H VV +K+YG +SW +L +I H ++
Sbjct: 83 DH-FGSILGVLRGCLCISQYHKETHSFVVSQMKEYGVNESWTQLFNIIVHERI------- 238
Query: 334 IHASCCLRPLCISENG--IVFMRTIDLQLVSYNTKNGSSDDPEIN---MGRYRRDDMHIY 388
+H+ C +C+SENG ++F + Q V YN ++ +D +I +G Y R+ Y
Sbjct: 239 LHSPCF--AMCMSENGDVLLFAESGVSQAVLYNQRDNKLEDTKIANNIVGSYVRN----Y 400
Query: 389 HESLISP 395
ESLISP
Sbjct: 401 IESLISP 421
Score = 20.8 bits (42), Expect(2) = 3e-07
Identities = 8/9 (88%), Positives = 9/9 (99%)
Frame = +1
Query: 255 IISFDLEKE 263
I+SFDLEKE
Sbjct: 1 IVSFDLEKE 27
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.139 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,263,566
Number of Sequences: 28460
Number of extensions: 161942
Number of successful extensions: 1356
Number of sequences better than 10.0: 96
Number of HSP's better than 10.0 without gapping: 1327
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1338
length of query: 396
length of database: 4,897,600
effective HSP length: 92
effective length of query: 304
effective length of database: 2,279,280
effective search space: 692901120
effective search space used: 692901120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0314.20