BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.17
(1113 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17449 238 3e-83
TC7972 similar to GB|AAO63356.1|28950865|BT005292 At4g16580 {Ara... 73 2e-13
TC10196 homologue to UP|Q39804 (Q39804) BiP isoform B, partial (... 37 0.013
TC11001 homologue to UP|Q9LYF9 (Q9LYF9) Clathrin binding protein... 36 0.029
TC19379 homologue to UP|Q945P0 (Q945P0) Transcriptional activato... 35 0.085
TC19018 similar to UP|CGS5_YEAST (P30283) S-phase entry cyclin 5... 33 0.25
TC17373 similar to GB|AAO63356.1|28950865|BT005292 At4g16580 {Ar... 33 0.32
TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa pro... 32 0.42
TC18517 weakly similar to UP|AAQ82688 (AAQ82688) Epa4p, partial ... 32 0.55
TC9123 similar to UP|O48879 (O48879) Ribosomal protein L22 (Frag... 31 0.94
AV776277 30 1.6
TC9195 similar to UP|ISPF_ARATH (Q9CAK8) 2-C-methyl-D-erythritol... 30 2.1
TC7994 similar to UP|Q8RU73 (Q8RU73) Ribose-5-phosphate isomeras... 30 2.7
TC13243 29 4.7
BP058074 28 6.1
AW719663 28 6.1
TC17732 similar to UP|O80362 (O80362) Chloroplast ribosomal prot... 28 6.1
>TC17449
Length = 741
Score = 238 bits (606), Expect(2) = 3e-83
Identities = 119/120 (99%), Positives = 120/120 (99%)
Frame = +2
Query: 894 STIKPAEVLTRSAAETQTPGSSSVLVAYFDGEALHAANVGNTGFIIIRDGFIFKKSTAKF 953
STIKP+EVLTRSAAETQTPGSSSVLVAYFDGEALHAANVGNTGFIIIRDGFIFKKSTAKF
Sbjct: 2 STIKPSEVLTRSAAETQTPGSSSVLVAYFDGEALHAANVGNTGFIIIRDGFIFKKSTAKF 181
Query: 954 HEFNFPLHIVKGDDPSEIIEGYKVDLCDGDVIIFATNGLFDNLYDQEIASTISKSLQASL 1013
HEFNFPLHIVKGDDPSEIIEGYKVDLCDGDVIIFATNGLFDNLYDQEIASTISKSLQASL
Sbjct: 182 HEFNFPLHIVKGDDPSEIIEGYKVDLCDGDVIIFATNGLFDNLYDQEIASTISKSLQASL 361
Score = 89.7 bits (221), Expect(2) = 3e-83
Identities = 45/47 (95%), Positives = 46/47 (97%)
Frame = +1
Query: 1013 LGPQEIAEVLAKRAQEVGKSTSTRSPFADAAQAIGYVGYSGGKLDDL 1059
L PQEIAEVLAKRAQEVGKSTSTRSPFADAAQAIGYVGYSGGKLDD+
Sbjct: 358 LVPQEIAEVLAKRAQEVGKSTSTRSPFADAAQAIGYVGYSGGKLDDV 498
>TC7972 similar to GB|AAO63356.1|28950865|BT005292 At4g16580 {Arabidopsis
thaliana;}, partial (35%)
Length = 510
Score = 73.2 bits (178), Expect = 2e-13
Identities = 40/111 (36%), Positives = 65/111 (58%), Gaps = 2/111 (1%)
Frame = +1
Query: 951 AKFHEFNFPLHIVKGDDPSEIIEG--YKVDLCDGDVIIFATNGLFDNLYDQEIASTISKS 1008
A+ H+FN P + +G++ G + + + GDVI+ T+GLFDNL++ EI + + +
Sbjct: 4 AQQHDFNCPYQLERGNEGDLPSSGEVFTLPVAPGDVIVAGTDGLFDNLHNDEITAVVEHA 183
Query: 1009 LQASLGPQEIAEVLAKRAQEVGKSTSTRSPFADAAQAIGYVGYSGGKLDDL 1059
+ L P A+ +A A+E ++PF+ AAQ +G+ Y GGKLDDL
Sbjct: 184 AKMGLEPHMTAQKIAALARERALDKFGQTPFSTAAQKVGF-PYYGGKLDDL 333
>TC10196 homologue to UP|Q39804 (Q39804) BiP isoform B, partial (17%)
Length = 562
Score = 37.4 bits (85), Expect = 0.013
Identities = 27/80 (33%), Positives = 42/80 (51%), Gaps = 3/80 (3%)
Frame = +2
Query: 369 EVEEKLDGDEEDAISTVTPESDMFSDMNNGASGE-VEEKLDFHEA--DALSTDTPGSNVN 425
++ +KL+ DE++ I T E+ + D N A E EEKL EA + + T +
Sbjct: 107 KLADKLESDEKEKIETAVKEALEWLDDNQSAEKEEYEEKLKEVEAVCNPIITAVYQRSGG 286
Query: 426 SDMNNGASGEVEEKIDDHEE 445
+ GASGE E++ D H+E
Sbjct: 287 APGGEGASGEGEDEDDSHDE 346
>TC11001 homologue to UP|Q9LYF9 (Q9LYF9) Clathrin binding protein-like,
partial (5%)
Length = 652
Score = 36.2 bits (82), Expect = 0.029
Identities = 33/130 (25%), Positives = 59/130 (45%), Gaps = 27/130 (20%)
Frame = +3
Query: 422 SNVNSDMNNGASG----EVEEKIDDHEEASVSSITPESDMFTDISSGALGEVD------- 470
+N NS + + +SG E+ DD + S T SSG+ +VD
Sbjct: 117 TNANSSVPSQSSGVPVNSTEDDFDDFDPRGTS---------TKASSGSSNQVDLFGQDLI 269
Query: 471 -EKLDVPEEVAV----SAVTPESDMFSDMN---------NGSAGEVEEKLD--HEEVAVS 514
+ +D P V+V ++ E D+F+D + G++ + ++K+D + A+
Sbjct: 270 GDLMDAPASVSVENPATSDVSEVDLFADASFVSAEPHADKGASSQPQDKVDLFSSQPAIP 449
Query: 515 TVTPEADMFT 524
TVTP D+F+
Sbjct: 450 TVTPTVDLFS 479
Score = 35.8 bits (81), Expect = 0.038
Identities = 33/122 (27%), Positives = 58/122 (47%), Gaps = 27/122 (22%)
Frame = +3
Query: 299 NSGASGEVEEEVDGLAVSAATQELDMFGDLN-----SGGALGEVE---EKLDGDEDDAIS 350
++ A+ V + G+ V++ + D F S G+ +V+ + L GD DA +
Sbjct: 114 STNANSSVPSQSSGVPVNSTEDDFDDFDPRGTSTKASSGSSNQVDLFGQDLIGDLMDAPA 293
Query: 351 TVTPES---------DMFSDLN---------NGASGEVEEKLD-GDEEDAISTVTPESDM 391
+V+ E+ D+F+D + GAS + ++K+D + AI TVTP D+
Sbjct: 294 SVSVENPATSDVSEVDLFADASFVSAEPHADKGASSQPQDKVDLFSSQPAIPTVTPTVDL 473
Query: 392 FS 393
FS
Sbjct: 474 FS 479
Score = 30.0 bits (66), Expect = 2.1
Identities = 47/207 (22%), Positives = 74/207 (35%), Gaps = 6/207 (2%)
Frame = +3
Query: 120 SIEILNNEVDDNLQEESKSSSIVVVGVADQNPQIPINEKESVVLDNESERTVINESQKID 179
S L+ D SKSS+ V Q+ +P+N E D + T S
Sbjct: 57 SARSLSKSQDKMSSRVSKSSTNANSSVPSQSSGVPVNSTEDDFDDFDPRGTSTKAS---- 224
Query: 180 RHLKLDSIEDGDAQHGILSEDGAAECNGVP-SVCEEESQADDHKEVVVSRTVTAVSDVFS 238
Q + +D + P SV E D EV + + VS
Sbjct: 225 --------SGSSNQVDLFGQDLIGDLMDAPASVSVENPATSDVSEVDLFADASFVS-AEP 377
Query: 239 DINGGASEEVEEKVD-GQEEVVVSAVNPELDMFG--DPVSSAFDGHKEVVSAVTPELDVF 295
+ GAS + ++KVD + + V P +D+F +PV + + V D F
Sbjct: 378 HADKGASSQPQDKVDLFSSQPAIPTVTPTVDLFSIPEPVVQPDNKSENYVPVNNSTFDPF 557
Query: 296 GG--LNSGASGEVEEEVDGLAVSAATQ 320
LN+ +V E + S ++Q
Sbjct: 558 AAVPLNTFDGSDVFGEFSSQSDSVSSQ 638
>TC19379 homologue to UP|Q945P0 (Q945P0) Transcriptional activator FHA1,
partial (56%)
Length = 525
Score = 34.7 bits (78), Expect = 0.085
Identities = 29/103 (28%), Positives = 47/103 (45%), Gaps = 2/103 (1%)
Frame = -2
Query: 556 FSDMNNGSAGEVEEKLDHEEVATVSTVTREADMFTDINNGAPGEVEEKLDDKEEVSVSTV 615
F + G+ GEVE + + + V T +R+ A E+ D +EEV +
Sbjct: 497 FLNHGTGAGGEVEVVMRRKWLMGVVTRSRKG--------AAAATAEDAADGEEEVKLLIP 342
Query: 616 TLEADMFTDMNSSAPGEVDEKLDGHEEVADLTSG--ASGEVEE 656
LE + +++ GEV + LD +A+ G A+GEV E
Sbjct: 341 DLEEVLGIELDRRVSGEVKDALDEAAVLAENLEGESAAGEVVE 213
Score = 30.0 bits (66), Expect = 2.1
Identities = 30/111 (27%), Positives = 46/111 (41%), Gaps = 5/111 (4%)
Frame = -2
Query: 366 ASGEVEEKLDGDEEDAISTVTPESDMFSDMNNGASGEVEEKLDFHEADALSTDTPGSNVN 425
A+ E+ DG+EE + E + +++ SGEV++ LD EA L+ + G +
Sbjct: 401 AAATAEDAADGEEEVKLLIPDLEEVLGIELDRRVSGEVKDALD--EAAVLAENLEGES-- 234
Query: 426 SDMNNGASGEVEEKI-----DDHEEASVSSITPESDMFTDISSGALGEVDE 471
A+GEV E D H A + D+ G LG E
Sbjct: 233 ------AAGEVVEDAGVVAGDVHAAAEAGEV--------DVDGGFLGVAAE 123
Score = 28.9 bits (63), Expect = 4.7
Identities = 29/106 (27%), Positives = 44/106 (41%)
Frame = -2
Query: 300 SGASGEVEEEVDGLAVSAATQELDMFGDLNSGGALGEVEEKLDGDEDDAISTVTPESDMF 359
+GA GEVE V + + G A E+ DG+E+ + E +
Sbjct: 482 TGAGGEVE------VVMRRKWLMGVVTRSRKGAAAATAEDAADGEEEVKLLIPDLEEVLG 321
Query: 360 SDLNNGASGEVEEKLDGDEEDAISTVTPESDMFSDMNNGASGEVEE 405
+L+ SGEV++ LD E A+ E + A+GEV E
Sbjct: 320 IELDRRVSGEVKDALD---EAAVLAENLEGE-------SAAGEVVE 213
>TC19018 similar to UP|CGS5_YEAST (P30283) S-phase entry cyclin 5, partial
(4%)
Length = 828
Score = 33.1 bits (74), Expect = 0.25
Identities = 31/96 (32%), Positives = 46/96 (47%), Gaps = 13/96 (13%)
Frame = -3
Query: 20 PSKHSEQ-FRISAPF-----PFPFPRRRISLTHHTHLVSNASSSSSSVPDDVDILSSTEC 73
P K ++ FR +P+ P P +SL T S++SS SSS P V +LSS
Sbjct: 334 PQKSEQRPFRSQSPYLVGSGPLGPPTSPVSL--FTSTSSSSSSPSSSSPSRVCLLSSGSD 161
Query: 74 SDGSFVFQFAKAS-------EIRDKLAELEREKLAC 102
S GS S E R+++ ELE ++++C
Sbjct: 160 SRGSCACSVTGFSVCC*VEEEQRNRVTELETDRVSC 53
>TC17373 similar to GB|AAO63356.1|28950865|BT005292 At4g16580 {Arabidopsis
thaliana;}, partial (15%)
Length = 857
Score = 32.7 bits (73), Expect = 0.32
Identities = 15/38 (39%), Positives = 21/38 (54%)
Frame = +1
Query: 843 GREDAYFISHQNWVAVADGVGQWSPEGNNSGPYIRELM 880
G +H+ + VADGVG W+ G N+ Y +ELM
Sbjct: 694 GERMLILFAHEQVIGVADGVGGWANVGVNAVLYAQELM 807
Score = 32.3 bits (72), Expect = 0.42
Identities = 20/62 (32%), Positives = 31/62 (49%), Gaps = 2/62 (3%)
Frame = +3
Query: 796 DYESSSHLTVPELHSVEMASYGENTSRTELFLVSAAACLPHPSKALTGREDAYFIS--HQ 853
D + ++ T+P + +V S L +++ + LPHP K TG EDA+FI
Sbjct: 579 DEQLANSSTLPNITTV---------SGKTLKMIAGSCYLPHPDKVETGGEDAHFICT*TS 731
Query: 854 NW 855
NW
Sbjct: 732 NW 737
>TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa protein,
mitochondrial precursor, partial (74%)
Length = 1752
Score = 32.3 bits (72), Expect = 0.42
Identities = 22/75 (29%), Positives = 36/75 (47%)
Frame = +2
Query: 442 DHEEASVSSITPESDMFTDISSGALGEVDEKLDVPEEVAVSAVTPESDMFSDMNNGSAGE 501
D E ++ I +D +LGE +K +P EVA S++ + M+ +A E
Sbjct: 1190 DQERKALIDIRNSADTSIYSIEKSLGEYRDK--IPSEVAKEIEDAVSELRTAMSGDNADE 1363
Query: 502 VEEKLDHEEVAVSTV 516
++ KLD AVS +
Sbjct: 1364 IKSKLDAANKAVSKI 1408
Score = 31.2 bits (69), Expect = 0.94
Identities = 21/72 (29%), Positives = 35/72 (48%)
Frame = +2
Query: 501 EVEEKLDHEEVAVSTVTPEADMFTDVNSGALGEVDEKLDVPEEVAVSAVTPESDMFSDMN 560
E+ + D E A+ + AD +LGE +K +P EVA S++ + M+
Sbjct: 1172 ELHAQKDQERKALIDIRNSADTSIYSIEKSLGEYRDK--IPSEVAKEIEDAVSELRTAMS 1345
Query: 561 NGSAGEVEEKLD 572
+A E++ KLD
Sbjct: 1346 GDNADEIKSKLD 1381
>TC18517 weakly similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (4%)
Length = 493
Score = 32.0 bits (71), Expect = 0.55
Identities = 26/93 (27%), Positives = 42/93 (44%), Gaps = 2/93 (2%)
Frame = -2
Query: 166 ESERTVINESQKIDRHLKLDSIEDGDAQHGILSEDGAAECNGVPSVCEEESQADDHKEVV 225
E E + + DR + + E+ + G +DG +GVP +EE + +DH V
Sbjct: 432 EDETVIDGAGEDNDRAVAEEEEEEPGEEDGEEDDDG----DGVPDEGDEEDEEEDHGVV- 268
Query: 226 VSRTVTAVSDVFSDINGGASEEVE--EKVDGQE 256
V++V GG +E V E V+G+E
Sbjct: 267 ----AEEVAEVVLHAEGGFAEGVRAGEGVEGEE 181
>TC9123 similar to UP|O48879 (O48879) Ribosomal protein L22 (Fragment),
partial (85%)
Length = 942
Score = 31.2 bits (69), Expect = 0.94
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = +1
Query: 29 ISAPFPFPFPRRRISLTHHTHLVSNASSSSSSVPDDVDILSSTECSDGSF 78
+S+ +PFP P +S HH+ L N SS+S P D L + SF
Sbjct: 64 VSSEWPFPSPSTALSSLHHS-LPPNPSSNSLHAPSPFDALLPLSPTKPSF 210
>AV776277
Length = 480
Score = 30.4 bits (67), Expect = 1.6
Identities = 13/29 (44%), Positives = 16/29 (54%)
Frame = -2
Query: 22 KHSEQFRISAPFPFPFPRRRISLTHHTHL 50
+H R S+PFPFP R + L HH L
Sbjct: 479 RHHHIHRNSSPFPFPLRSRSMLLLHHRRL 393
>TC9195 similar to UP|ISPF_ARATH (Q9CAK8) 2-C-methyl-D-erythritol
2,4-cyclodiphosphate synthase, chloroplast precursor
(MECPS) (MECDP-synthase) , partial (72%)
Length = 1077
Score = 30.0 bits (66), Expect = 2.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 19 VPSKHSEQFRISAPFPFPFPRRRISLTHHTHLVSNASSSSSS 60
VP K + + +PF F FPR+ HHTH+ S S ++S
Sbjct: 116 VPFKITSSSSLLSPFSFSFPRK-----HHTHVPSLRLSPTAS 226
>TC7994 similar to UP|Q8RU73 (Q8RU73) Ribose-5-phosphate isomerase
precursor , partial (81%)
Length = 1355
Score = 29.6 bits (65), Expect = 2.7
Identities = 33/141 (23%), Positives = 52/141 (36%), Gaps = 11/141 (7%)
Frame = -3
Query: 168 ERTVINESQKIDRHLKLDSIEDGDAQHGILSEDGAAECNGVPSVCEE--ESQADDHKEVV 225
E++ +++ + L DG+ + GI+ EDG + G + ADD E+
Sbjct: 708 EKSAAATLDEVEERVDLVGAVDGEIEAGIVVEDGERDAEGSRLLLRALGGGDADDVGELA 529
Query: 226 VSRTVTAVSDVFSDINGGASEEVEEK---------VDGQEEVVVSAVNPELDMFGDPVSS 276
+ + D + GGA E E+ V G+ VV D FG +
Sbjct: 528 GGEKLAELGD--DEGGGGAGAEAEDHAGLDVVDGLVGGESLEVVLGEGDGTDGFGSGGGA 355
Query: 277 AFDGHKEVVSAVTPELDVFGG 297
F G V + V GG
Sbjct: 354 *FKGSGGA*DKVGGGIVVCGG 292
>TC13243
Length = 449
Score = 28.9 bits (63), Expect = 4.7
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -2
Query: 32 PFPFPFPRRRISLTHH 47
PFPFPFP RR L H
Sbjct: 106 PFPFPFPSRRFPLVLH 59
>BP058074
Length = 575
Score = 28.5 bits (62), Expect = 6.1
Identities = 22/73 (30%), Positives = 30/73 (40%)
Frame = -3
Query: 991 GLFDNLYDQEIASTISKSLQASLGPQEIAEVLAKRAQEVGKSTSTRSPFADAAQAIGYVG 1050
GL NLYD A+ S SLQ + + V A R + T S F + A
Sbjct: 414 GLIVNLYDNRFAAQRSISLQINAYRSPSSSVFASRQNGMSNQTVENSGFTSSDTA----- 250
Query: 1051 YSGGKLDDLKTSG 1063
+ DD++ SG
Sbjct: 249 ----ESDDVEESG 223
>AW719663
Length = 474
Score = 28.5 bits (62), Expect = 6.1
Identities = 32/151 (21%), Positives = 61/151 (40%), Gaps = 9/151 (5%)
Frame = +1
Query: 79 VFQFAKASEIRDKLAELEREKLACEVVEGGGKVGIQALLSDS--IEILNNEVDDNLQEES 136
V+Q +E K + E K +VV G K + L+ ++ ++ + E D+ + ES
Sbjct: 19 VYQIKHTNE--KKASYGESAKTGNDVVRLGRK-DLNPLVEETTVVDARHKEQDEESEHES 189
Query: 137 KSSSIV---VVGVADQNPQIPINEKESVVLDNESERTVINESQKIDRHLKLDSI----ED 189
K+ + V D+N E+E + LD+ + K + H+KLD E+
Sbjct: 190 KTEEQINLDEVNSVDENEPEHKPEEEHIKLDDANGVDENEVENKPEEHIKLDDANGVDEN 369
Query: 190 GDAQHGILSEDGAAECNGVPSVCEEESQADD 220
+ ++ +G + E E +
Sbjct: 370 SEHSQDLVEHEGEGSSENARTDIESEQHVXE 462
>TC17732 similar to UP|O80362 (O80362) Chloroplast ribosomal protein L10,
partial (77%)
Length = 903
Score = 28.5 bits (62), Expect = 6.1
Identities = 25/70 (35%), Positives = 30/70 (42%)
Frame = +3
Query: 17 RNVPSKHSEQFRISAPFPFPFPRRRISLTHHTHLVSNASSSSSSVPDDVDILSSTECSDG 76
R+ PS S +P PFP PRRR S AS SSS + S+T+ S
Sbjct: 111 RDPPSNPSATASHQSPPPFPAPRRRKQ--------STASGSSSRIATS-SPESTTKASPS 263
Query: 77 SFVFQFAKAS 86
S F K S
Sbjct: 264 SSSRSFGKRS 293
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.307 0.127 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,688,355
Number of Sequences: 28460
Number of extensions: 187252
Number of successful extensions: 922
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 864
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 915
length of query: 1113
length of database: 4,897,600
effective HSP length: 100
effective length of query: 1013
effective length of database: 2,051,600
effective search space: 2078270800
effective search space used: 2078270800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0314.17