BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.16
(185 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17770 weakly similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, ... 229 2e-61
TC9144 67 2e-12
TC15783 similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3g22600/F... 64 1e-11
AV417442 53 4e-08
TC19430 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lip... 42 5e-05
TC9111 42 8e-05
BP055303 41 1e-04
BP039620 41 1e-04
TC9112 weakly similar to UP|Q9SI70 (Q9SI70) F23N19.16, partial (... 40 2e-04
TC13156 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lip... 40 3e-04
AI967893 39 7e-04
TC10327 weakly similar to UP|NLT1_PRUDU (Q43017) Nonspecific lip... 36 0.005
CN825044 35 0.006
BP055578 33 0.030
TC10431 similar to UP|Q7XTF6 (Q7XTF6) OJ991214_12.11 protein, pa... 31 0.11
AI967923 31 0.15
TC17419 weakly similar to UP|Q17796 (Q17796) Prion-like-(q/n-ric... 30 0.33
BP038129 29 0.43
CB828155 28 0.73
CB828923 28 1.2
>TC17770 weakly similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, partial
(42%)
Length = 554
Score = 229 bits (584), Expect = 2e-61
Identities = 114/115 (99%), Positives = 114/115 (99%)
Frame = +3
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG
Sbjct: 174 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 353
Query: 61 QCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
QCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLN TKALALPSACKVSASNSVTNCG
Sbjct: 354 QCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNATKALALPSACKVSASNSVTNCG 518
>TC9144
Length = 835
Score = 67.0 bits (162), Expect = 2e-12
Identities = 34/100 (34%), Positives = 51/100 (51%), Gaps = 3/100 (3%)
Frame = +1
Query: 9 LCVVAICAVDFAHSASSNNAPAPS---ADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSG 65
L + C A S + +A APS DC + ++DCL+FV S +TKP CC
Sbjct: 118 LVLALACHGMSAQSLAPESASAPSPAGVDCFTALTNVSDCLTFVEAGSNLTKPDKGCCPE 297
Query: 66 LKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKV 105
++++ P CLC+ G+ +N+ KA+ LPS C V
Sbjct: 298 FAGLIESNPICLCQLLGKPDFVGIKINLNKAIKLPSVCGV 417
>TC15783 similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3g22600/F16J14_17),
partial (48%)
Length = 578
Score = 64.3 bits (155), Expect = 1e-11
Identities = 29/77 (37%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Frame = -2
Query: 30 APSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFK-SSSQFG 88
A + C++ + ++ CL+++T S+ P CCS L SV++++P CLC S FG
Sbjct: 376 AAQSGCTSALTSLSPCLNYITGSSS--SPSPSCCSQLSSVVQSSPQCLCSLLNGGGSSFG 203
Query: 89 VVLNVTKALALPSACKV 105
+ +N T AL+LP CKV
Sbjct: 202 ITMNQTLALSLPGPCKV 152
>AV417442
Length = 391
Score = 52.8 bits (125), Expect = 4e-08
Identities = 25/69 (36%), Positives = 36/69 (51%), Gaps = 1/69 (1%)
Frame = +3
Query: 36 SNLILKMTDCLSFVTNDS-TVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVT 94
S + T C+SF+TN S T P +CC +KS+ CLC + F + +N T
Sbjct: 183 SAISTSFTPCMSFLTNSSGNGTSPTAECCDSIKSLTSGGRDCLCLVVTGNVPFSIPINRT 362
Query: 95 KALALPSAC 103
A++LP AC
Sbjct: 363 LAISLPRAC 389
>TC19430 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lipid-transfer
protein 3 precursor (LTP 3) (CW20) (CW-20) (CW-19),
partial (53%)
Length = 520
Score = 42.4 bits (98), Expect = 5e-05
Identities = 25/91 (27%), Positives = 46/91 (50%), Gaps = 5/91 (5%)
Frame = +3
Query: 27 NAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAF 81
+A + CS++I + C+S++ + S KP G CCSG K++ + C
Sbjct: 72 SASEAAISCSDVIKDLKPCVSYLVSGSG--KPPGACCSGAKALASAVSTSEDKKAACNCI 245
Query: 82 KSSSQFGVVLNVTKALALPSACKVSASNSVT 112
KS+++ + +N A ALP C ++ +V+
Sbjct: 246 KSTAK-SIKMNSQLAKALPGNCGINVPINVS 335
>TC9111
Length = 534
Score = 41.6 bits (96), Expect = 8e-05
Identities = 30/115 (26%), Positives = 53/115 (46%), Gaps = 1/115 (0%)
Frame = +2
Query: 2 ASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQ 61
A + ++ V I A+ F+ + + ++ P+ + NLIL C ++T+ T P
Sbjct: 68 AMPLMMVALVFTIGAIFFSTAQAQSSIPSCA---QNLIL----CADYLTS----TNPPAS 214
Query: 62 CCSGLKSVLKTAPSCLCEAFKSSSQF-GVVLNVTKALALPSACKVSASNSVTNCG 115
CC +K ++T +CLC F S ++ +AL L C V++ S G
Sbjct: 215 CCDAIKKTVETQLTCLCNLFYSPGLLQSFKISTDQALQLSRRCGVTSDLSSCKSG 379
>BP055303
Length = 515
Score = 41.2 bits (95), Expect = 1e-04
Identities = 23/54 (42%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
Frame = -1
Query: 64 SGLKSVLKTAPSCLCEAFK---SSSQFGVVLNVTKALALPSACKVSASNSVTNC 114
S L V+K+ P CLCE SS + +N T+AL LPSAC V T C
Sbjct: 515 SQLSFVVKSQPQCLCEVVNGGASSIAASLNINQTQALTLPSACNVQTPPITTTC 354
>BP039620
Length = 503
Score = 41.2 bits (95), Expect = 1e-04
Identities = 27/103 (26%), Positives = 44/103 (42%)
Frame = +3
Query: 3 SKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQC 62
S+V+++L V + S + N+ C+N + + C FV ++ TKP C
Sbjct: 60 SQVAVLLLXVVVLGTKTEMSEAQNS-------CTNQLTNLNVCAPFVVPGASNTKPSADC 218
Query: 63 CSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKV 105
C L +V CLC + +SQ LPS C++
Sbjct: 219 CGALSAV---NHECLCSTLRIASQ------------LPSQCQL 302
>TC9112 weakly similar to UP|Q9SI70 (Q9SI70) F23N19.16, partial (53%)
Length = 807
Score = 40.0 bits (92), Expect = 2e-04
Identities = 28/115 (24%), Positives = 50/115 (43%), Gaps = 1/115 (0%)
Frame = +1
Query: 2 ASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQ 61
A + ++ V I A+ F+ + + ++ P+ C+ ++ D L T T P
Sbjct: 88 AMPLMMVALVFTIGAIFFSTAQAQSSIPS----CAQNLIPCADHL-------TSTNPPAS 234
Query: 62 CCSGLKSVLKTAPSCLCEAFKSSSQF-GVVLNVTKALALPSACKVSASNSVTNCG 115
CC +K ++T +CLC F S ++ +AL L C V++ S G
Sbjct: 235 CCDAIKKTVETQLTCLCNLFYSPGLLQSFKISTDQALQLSRRCGVTSDLSSCKSG 399
>TC13156 weakly similar to UP|NLT3_HORVU (Q43766) Nonspecific lipid-transfer
protein 3 precursor (LTP 3) (CW20) (CW-20) (CW-19),
partial (53%)
Length = 603
Score = 39.7 bits (91), Expect = 3e-04
Identities = 25/91 (27%), Positives = 45/91 (48%), Gaps = 5/91 (5%)
Frame = +1
Query: 27 NAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPS-----CLCEAF 81
+A + CS++I + C+S++ + S KP CCSG K++ + C
Sbjct: 61 SASEAAISCSDVIKDLKPCVSYLVSGS--GKPPVACCSGAKALASAVSTSEDKKAACNCI 234
Query: 82 KSSSQFGVVLNVTKALALPSACKVSASNSVT 112
KS+++ + +N A ALP C ++ SV+
Sbjct: 235 KSTAK-SIKMNSQLAEALPGNCGINTPISVS 324
>AI967893
Length = 258
Score = 38.5 bits (88), Expect = 7e-04
Identities = 21/71 (29%), Positives = 40/71 (55%), Gaps = 1/71 (1%)
Frame = +3
Query: 5 VSLILCVVA-ICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCC 63
+ LIL V+A +CA A + C+N+++ ++ CL+++T +S+ P CC
Sbjct: 84 MGLILVVMAMLCA-----------GAAAQSSCTNVLVSLSPCLNYITGNSST--PSSGCC 224
Query: 64 SGLKSVLKTAP 74
S L +V+++ P
Sbjct: 225 SQLATVVRSQP 257
>TC10327 weakly similar to UP|NLT1_PRUDU (Q43017) Nonspecific lipid-transfer
protein 1 precursor (LTP 1), partial (52%)
Length = 683
Score = 35.8 bits (81), Expect = 0.005
Identities = 28/126 (22%), Positives = 52/126 (41%), Gaps = 10/126 (7%)
Frame = +1
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
+A VSL + + AV A + C ++ + C+S++ N + G
Sbjct: 82 VARLVSLAIVFAVLVAVTTVPRAEG------AVTCGQVVNSLYPCVSYLMNGGNMVP--G 237
Query: 61 QCCSGLKSVLKTAP---------SCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSV 111
QCC+G++++ A +C+ +A +S N+ A LP C V+ +
Sbjct: 238 QCCNGIRNLYGMATNTPDRRAVCTCIKQAVSNSGFSFTSYNLNLAAGLPKKCGVNIPYQI 417
Query: 112 T-NCGC 116
+ N C
Sbjct: 418 SPNTDC 435
>CN825044
Length = 530
Score = 35.4 bits (80), Expect = 0.006
Identities = 24/79 (30%), Positives = 34/79 (42%)
Frame = +3
Query: 37 NLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKA 96
NL +T C +FV + T P CC LK V CLC+ + +++ KA
Sbjct: 96 NLTAIVTQCANFVKKEGTKIPPSSACCETLKDV---DIPCLCKHI--TPPIASQISMEKA 260
Query: 97 LALPSACKVSASNSVTNCG 115
+ + C V S T CG
Sbjct: 261 VYVAKTCGVEVP-SGTKCG 314
>BP055578
Length = 506
Score = 33.1 bits (74), Expect = 0.030
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
Frame = +1
Query: 38 LILKMTDCLSFVT---NDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVT 94
L+ + CL + + N+ T T G CC S C C+ + +S G LN T
Sbjct: 112 LMSPFSPCLPYFSSPPNNRTETA-SGACCHAFSSATTVNSLCFCQLLRDASILGFPLNTT 288
Query: 95 KALAL 99
+ L+L
Sbjct: 289 RLLSL 303
>TC10431 similar to UP|Q7XTF6 (Q7XTF6) OJ991214_12.11 protein, partial (38%)
Length = 582
Score = 31.2 bits (69), Expect = 0.11
Identities = 23/108 (21%), Positives = 42/108 (38%)
Frame = +2
Query: 8 ILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLK 67
IL ++ ++ S + S N +K+ C S +++ CC+ ++
Sbjct: 74 ILGLLVFVSISEIQSVEAAGECGRSTTPDNEAMKLIPCASAAQDENASVSQS--CCAQVQ 247
Query: 68 SVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
+ K PSCLC S+ +N A+ +P C + CG
Sbjct: 248 KIGKN-PSCLCAVVLSNMAKMSGVNPKIAITIPKRCNLDNRPIGYKCG 388
>AI967923
Length = 302
Score = 30.8 bits (68), Expect = 0.15
Identities = 18/62 (29%), Positives = 25/62 (40%), Gaps = 3/62 (4%)
Frame = +3
Query: 45 CLSFVT---NDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPS 101
CL +V+ N+ T T CC + C C + G LN T+ L+L S
Sbjct: 69 CLPYVSSPPNNLTGTASTA-CCDAFSAATAVDSLCFCHLLRDPVILGFSLNTTRLLSLSS 245
Query: 102 AC 103
C
Sbjct: 246 VC 251
>TC17419 weakly similar to UP|Q17796 (Q17796)
Prion-like-(q/n-rich)-domain-bearing protein protein 9,
partial (5%)
Length = 621
Score = 29.6 bits (65), Expect = 0.33
Identities = 29/104 (27%), Positives = 42/104 (39%), Gaps = 2/104 (1%)
Frame = +2
Query: 12 VAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQC--CSGLKSV 69
V +CA D +++ S P S+D N I L N + T P SG+K
Sbjct: 197 VRVCA-DCFNNSRSQEVPHASSDGVNNITDTISKLDIDGNVDSKTLPSADNNPVSGIKEC 373
Query: 70 LKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTN 113
P C+CEA SS ALP K+S + + ++
Sbjct: 374 KCGMPLCICEAPAPSSD-----------ALPQQKKISPAMTASS 472
>BP038129
Length = 558
Score = 29.3 bits (64), Expect = 0.43
Identities = 23/57 (40%), Positives = 28/57 (48%), Gaps = 5/57 (8%)
Frame = +1
Query: 120 CLSLIIPHLGFLRFIFFCIKFGIFNLLLMKNYI-----PLWISCYVSNEVFFFLFCF 171
C SL H FLRF++FC F LL + YI PL + C N + FF F F
Sbjct: 16 CTSL--SHFHFLRFVWFC-AFSCSPLLSIPEYITSSITPLSLPC---NSMRFFKFGF 168
>CB828155
Length = 542
Score = 28.5 bits (62), Expect = 0.73
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1
Query: 101 SACKVSASNSVTNCGCEFFCLSLIIPHL 128
S C ++ +N T CGC+F+CL + H+
Sbjct: 262 SVCGLTVTNG-TLCGCQFWCLQSKLSHI 342
>CB828923
Length = 488
Score = 27.7 bits (60), Expect = 1.2
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +1
Query: 133 FIFFCIKFGIFNLLLMKNYIPLWISCYVSNEVFFFLFCF 171
FI I F ++++L++ + ++ + S VFFF FCF
Sbjct: 280 FIHTLIFFALYSILIIAVRVHIYTG*F-SRSVFFFFFCF 393
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,438,043
Number of Sequences: 28460
Number of extensions: 81351
Number of successful extensions: 796
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of query: 185
length of database: 4,897,600
effective HSP length: 85
effective length of query: 100
effective length of database: 2,478,500
effective search space: 247850000
effective search space used: 247850000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0314.16