BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.13
(643 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8045 weakly similar to UP|ST35_ARATH (Q94LW6) Probable sulfate... 332 9e-92
CB829305 213 8e-56
TC8880 similar to UP|ST35_ARATH (Q94LW6) Probable sulfate transp... 206 1e-53
TC11476 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate ... 117 3e-32
TC17837 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate ... 112 2e-25
BP037671 47 1e-05
AW720566 37 0.010
BI416414 30 0.92
BP064580 30 0.92
TC11730 30 1.2
AU088765 28 3.5
TC19668 weakly similar to UP|BYS_DROME (P51406) Bys protein, par... 28 4.6
TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 gluc... 27 7.8
>TC8045 weakly similar to UP|ST35_ARATH (Q94LW6) Probable sulfate
transporter 3.5, partial (39%)
Length = 1616
Score = 332 bits (852), Expect = 9e-92
Identities = 165/389 (42%), Positives = 257/389 (65%)
Frame = +3
Query: 245 WETIVIGMSFLVFILITNYIAKKNKKLFWVAAIAPMISVVVSTFCVYITRADKKGVAIVK 304
WET V+G+ FL F+ T ++ K KLFWV+AIAPM V+V + VY+ K G+ IV
Sbjct: 3 WETTVLGLVFLAFLQFTRHVRNKRPKLFWVSAIAPMTVVIVGSIFVYLVHGQKHGIPIVG 182
Query: 305 HIKKGVNPASASEIFFSGEYFGAGVKIGVVSGMVALTEAVAIARTFAAMKDYSIDGNKEM 364
H+ +G+NP S F +Y A ++ +++G+++L E +AI R+F+ + DGNKEM
Sbjct: 183 HLDRGLNPWSIQYFNFDSKYLPAVMQAALITGVLSLAEGIAIGRSFSVTDNTPHDGNKEM 362
Query: 365 VAMGTMNIVGSLTSSYVATGSFSRSAVNYMAGCKTAVSNIVMSMVLLLTLLVITPLFKYT 424
VA G MN+ GS TS Y+ +G FS++AVNY AG KTA++N+V ++++ LTL + PLF +T
Sbjct: 363 VAFGLMNLFGSFTSCYLTSGPFSKTAVNYNAGGKTAMTNVVQAVLMALTLQFLAPLFGFT 542
Query: 425 PNAVLASIIIAAVMNLVDVQAAILLWKIDKFDFVACMGAFFGVIFKSVEIGLLIAVAISF 484
P L++II +A++ LV+ I L+K+DKFDFV CM AF GV F ++ GL+I+V +
Sbjct: 543 PLVALSAIITSAMLGLVNYTEVIYLYKVDKFDFVICMAAFLGVAFLGMDYGLMISVGLGV 722
Query: 485 AKILLQVTRPRTAILGKLSGTKVYRNILQYPKATQIPGMLIIRVDSAIYFSNSNYIKDRI 544
+ LL V RP T LGKL+ +YR++ QYP +T PG++I+++ S +YFSNS Y+K+R+
Sbjct: 723 IRALLYVARPATCKLGKLNEFGIYRDVEQYPAST-FPGLIIVQLGSPVYFSNSVYVKERV 899
Query: 545 LKWVTDEEVQRTASEFPSIQSLIVEMSPVTDIDTSGIHALEDLFKSLKKREVQLLLANPG 604
++++ ++ S ++ +I++MS VT IDT+ I L +L K L+K +++ L NP
Sbjct: 900 MRYIKSQQ----RSNEDVVEQVILDMSGVTSIDTTAIEGLLELNKMLEKNGIEMFLVNPR 1067
Query: 605 PIVIEKLHASKLSDIIGEDKIFSSVDDAV 633
V+EKL SK D +G++ + ++DDAV
Sbjct: 1068LEVMEKLIISKFVDKLGKESFYLTLDDAV 1154
>CB829305
Length = 509
Score = 213 bits (542), Expect = 8e-56
Identities = 104/165 (63%), Positives = 135/165 (81%)
Frame = +3
Query: 329 VKIGVVSGMVALTEAVAIARTFAAMKDYSIDGNKEMVAMGTMNIVGSLTSSYVATGSFSR 388
+K G+V+G+++LTE +A+ RTFAA+++Y +DGNKEM+A+G MNI GS +S YV TGSFSR
Sbjct: 15 IKTGLVTGILSLTEGIAVGRTFAALRNYQVDGNKEMIAIGVMNIAGSCSSCYVTTGSFSR 194
Query: 389 SAVNYMAGCKTAVSNIVMSMVLLLTLLVITPLFKYTPNAVLASIIIAAVMNLVDVQAAIL 448
SAVNY AG +TAVSNI+M+ +L+TLL + PLF YTPN VLA+III AV+ L+D QAA
Sbjct: 195 SAVNYNAGARTAVSNIIMASAVLVTLLFLMPLFYYTPNVVLAAIIITAVIGLIDYQAAYK 374
Query: 449 LWKIDKFDFVACMGAFFGVIFKSVEIGLLIAVAISFAKILLQVTR 493
LWK+DK DF+AC+ +FFGV+F SV +GL IAVAIS KILL V+R
Sbjct: 375 LWKVDKLDFLACLCSFFGVLFISVPLGLGIAVAISVFKILLHVSR 509
>TC8880 similar to UP|ST35_ARATH (Q94LW6) Probable sulfate transporter 3.5,
partial (30%)
Length = 794
Score = 206 bits (523), Expect = 1e-53
Identities = 106/218 (48%), Positives = 142/218 (64%), Gaps = 2/218 (0%)
Frame = +3
Query: 20 HKVGTAPKQTLYLEIKHSVKETFFFDDPLSQFKGQ-SRKRKLVLGMQSVFPIVEWGRDYN 78
H V ++ Y ++K +KETFF DDP Q K + +R R+++ G+Q PI EW +Y
Sbjct: 123 HGVNFTAQRGFYTKLKSGLKETFFPDDPFRQIKEEENRSRRIIKGVQYYVPIFEWLPNYT 302
Query: 79 LQKFKGDFIAGLTIASLCIPQDIAYAKLANLNPEHALYTSFVAPLVYAFMGTSRDIAIGP 138
L+ F DFI+GLTI SL IPQ I+YAKLANL P LY+SFV PLVYA G+SR +A+G
Sbjct: 303 LRLFISDFISGLTITSLAIPQGISYAKLANLPPIVGLYSSFVPPLVYAIFGSSRHMAVGT 482
Query: 139 VAVVSLLLGTMLTDEIADSKNPD-YLRLAYTATFFAGITQLALGFFRLGFLIDFLSHAAI 197
+A SLL+G ++ + +P YL L +T TF G+ Q LG FRLG L+DF SH+ I
Sbjct: 483 LAAASLLIGQTISTVASPETDPTLYLHLIFTTTFVTGVFQACLGIFRLGILVDFFSHSTI 662
Query: 198 VGFMGGAAITIALQQLKGLLGLKTFTKKTDIVSVMRSV 235
GFMGG A + QQLKG G+K F+ KT++V V +S+
Sbjct: 663 TGFMGGTAFILIAQQLKGFFGMKHFSTKTNLVEVAKSI 776
>TC11476 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate transporter
3, partial (18%)
Length = 597
Score = 117 bits (294), Expect(2) = 3e-32
Identities = 54/87 (62%), Positives = 69/87 (79%)
Frame = +1
Query: 64 MQSVFPIVEWGRDYNLQKFKGDFIAGLTIASLCIPQDIAYAKLANLNPEHALYTSFVAPL 123
+QS+FPI+ W RDY KFK D +AGLT+ASLCIPQ I YA LA ++PE+ LYTS V PL
Sbjct: 217 LQSLFPILTWIRDYTFSKFKDDLLAGLTLASLCIPQSIGYAHLAQVDPEYGLYTSIVPPL 396
Query: 124 VYAFMGTSRDIAIGPVAVVSLLLGTML 150
+Y MG+SR+IAIGP AVVS+LL +++
Sbjct: 397 IYPLMGSSREIAIGPAAVVSMLLSSLV 477
Score = 38.1 bits (87), Expect(2) = 3e-32
Identities = 17/27 (62%), Positives = 19/27 (69%)
Frame = +2
Query: 162 YLRLAYTATFFAGITQLALGFFRLGFL 188
Y L +T TFF+GI Q A G FRLGFL
Sbjct: 515 YRNLIFTVTFFSGIFQAAFGIFRLGFL 595
>TC17837 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate transporter
3, partial (16%)
Length = 466
Score = 112 bits (280), Expect = 2e-25
Identities = 51/102 (50%), Positives = 75/102 (73%)
Frame = +1
Query: 285 VSTFCVYITRADKKGVAIVKHIKKGVNPASASEIFFSGEYFGAGVKIGVVSGMVALTEAV 344
+ST VY+++ADK GV I+KH+K G+NP+S ++ F G KIG++ ++ LTEA+
Sbjct: 1 LSTLIVYLSKADKIGVNILKHVKGGLNPSSVHQLQFHDPNVGQAAKIGLICAIIGLTEAI 180
Query: 345 AIARTFAAMKDYSIDGNKEMVAMGTMNIVGSLTSSYVATGSF 386
A+ R+FA+++ Y +DGN+EM++MG NI GSLTS YVATG F
Sbjct: 181 AVGRSFASIQGYHLDGNEEMLSMGVTNIAGSLTSCYVATGKF 306
>BP037671
Length = 468
Score = 46.6 bits (109), Expect = 1e-05
Identities = 26/61 (42%), Positives = 41/61 (66%), Gaps = 1/61 (1%)
Frame = -3
Query: 576 IDTSGIHALEDLFKSLKKREVQLLLANPGPIVIEKL-HASKLSDIIGEDKIFSSVDDAVA 634
IDTSGI ++ +++K+ V+L+L NP VIEKL A + ++ I ED +F +V +AVA
Sbjct: 460 IDTSGISFFKEFKSTMEKKGVELVLVNPLAEVIEKLKKADEANEFIREDNLFLTVGEAVA 281
Query: 635 T 635
+
Sbjct: 280 S 278
>AW720566
Length = 549
Score = 37.0 bits (84), Expect = 0.010
Identities = 19/47 (40%), Positives = 29/47 (61%)
Frame = +2
Query: 577 DTSGIHALEDLFKSLKKREVQLLLANPGPIVIEKLHASKLSDIIGED 623
DT+ I L +L K L+K +++ L NP V+EKL SK D +G++
Sbjct: 407 DTTAIEGLLELNKMLEKNGIEMFLVNPRLEVMEKLIISKFVDKLGKE 547
>BI416414
Length = 516
Score = 30.4 bits (67), Expect = 0.92
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +2
Query: 577 DTSGIHALEDLFKSLKKREVQLLLANPGPIVIEKL 611
DT+ I L +L K L+K +++ L NP V+EKL
Sbjct: 407 DTTAIEGLLELNKMLEKNGIEMFLVNPRLEVMEKL 511
>BP064580
Length = 538
Score = 30.4 bits (67), Expect = 0.92
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +3
Query: 267 KNKKLFWVAAIAPMISVVVST 287
K KLFWV+A P+ SV++ST
Sbjct: 471 KKPKLFWVSAAXPLTSVILST 533
>TC11730
Length = 914
Score = 30.0 bits (66), Expect = 1.2
Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 11/99 (11%)
Frame = +2
Query: 322 GEYFGAGVKIGVVSGMVALTEA-----------VAIARTFAAMKDYSIDGNKEMVAMGTM 370
G G G+ I VVS V L+E + T A +++ + +
Sbjct: 416 GLKLGLGLSIPVVSSGVILSEPKEELEVDECHDADWSDTEAQLEELVLSNLDAVFKSAIK 595
Query: 371 NIVGSLTSSYVATGSFSRSAVNYMAGCKTAVSNIVMSMV 409
IVG VAT + RS + Y GCK +SNIV +++
Sbjct: 596 KIVGCGYPEDVATKAILRSGICY--GCKDTISNIVDNII 706
>AU088765
Length = 423
Score = 28.5 bits (62), Expect = 3.5
Identities = 17/62 (27%), Positives = 32/62 (51%)
Frame = +1
Query: 508 YRNILQYPKATQIPGMLIIRVDSAIYFSNSNYIKDRILKWVTDEEVQRTASEFPSIQSLI 567
Y NI QY +A + + + Y+++RIL+WV +EE A+ S++ +I
Sbjct: 67 YYNINQYKEALRSSFISYFGCGVSNLLC*FPYLQERILRWVREEEELIKANNGXSLKCII 246
Query: 568 VE 569
++
Sbjct: 247 LD 252
>TC19668 weakly similar to UP|BYS_DROME (P51406) Bys protein, partial (12%)
Length = 646
Score = 28.1 bits (61), Expect = 4.6
Identities = 20/67 (29%), Positives = 32/67 (46%), Gaps = 1/67 (1%)
Frame = -1
Query: 128 MGTSRDIAI-GPVAVVSLLLGTMLTDEIADSKNPDYLRLAYTATFFAGITQLALGFFRLG 186
MGTS ++ + +++ GT + +I S LRL + + AG+T L F R
Sbjct: 298 MGTSGISSLFSSIGFINIETGTEI--DIRSSSFSPRLRLWFNSLLIAGVTNLCFCFSRRN 125
Query: 187 FLIDFLS 193
F + F S
Sbjct: 124 FKLSFCS 104
>TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 glucosidase
SCW10 precursor (Soluble cell wall protein 10) ,
partial (6%)
Length = 1209
Score = 27.3 bits (59), Expect = 7.8
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 3/50 (6%)
Frame = +2
Query: 321 SGEYFGA--GVKIGVVSGMVALTEAVAIARTFAAMKDYS-IDGNKEMVAM 367
+G FG+ G I +SG VA + A IAR FA + ++GNK+ +A+
Sbjct: 407 AGLLFGSVTGTVIVSISGTVAASVAFLIARYFARERILKLVEGNKKFLAV 556
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.138 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,025,769
Number of Sequences: 28460
Number of extensions: 105004
Number of successful extensions: 566
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 560
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 562
length of query: 643
length of database: 4,897,600
effective HSP length: 96
effective length of query: 547
effective length of database: 2,165,440
effective search space: 1184495680
effective search space used: 1184495680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0314.13