BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.12
(180 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13114 99 3e-22
TC11547 similar to UP|Q9SE03 (Q9SE03) Copper chaperone homolog C... 75 9e-15
TC16731 similar to UP|Q84KJ2 (Q84KJ2) Copper chaperone, partial ... 69 6e-13
BP034759 66 4e-12
BP049555 56 4e-09
TC19711 49 5e-07
CB828502 49 5e-07
TC13685 similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800),... 49 7e-07
BI418928 48 1e-06
TC16918 weakly similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g... 47 1e-06
TC12523 47 3e-06
BG662295 44 1e-05
AV418862 43 4e-05
BP052316 30 0.19
AV408400 28 0.71
TC16644 similar to UP|Q85UH4 (Q85UH4) Cytochrome oxidase subunit... 28 1.2
BP035425 27 1.6
TC12877 27 2.1
TC10030 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partia... 27 2.7
AV768715 25 6.0
>TC13114
Length = 504
Score = 99.4 bits (246), Expect = 3e-22
Identities = 49/49 (100%), Positives = 49/49 (100%)
Frame = +3
Query: 132 VKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG 180
VKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG
Sbjct: 3 VKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFWNG 149
>TC11547 similar to UP|Q9SE03 (Q9SE03) Copper chaperone homolog CCH, partial
(71%)
Length = 528
Score = 74.7 bits (182), Expect = 9e-15
Identities = 36/65 (55%), Positives = 48/65 (73%), Gaps = 1/65 (1%)
Frame = +1
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q VV++V + CQGCAG V R L KMEGV SFNID++ ++VTV G++ P VLE++SK K
Sbjct: 31 QTVVLKVRMSCQGCAGAVNRVLEKMEGVESFNIDLKEQKVTVKGNVKPEDVLETVSKSGK 210
Query: 174 RAEFW 178
+ FW
Sbjct: 211 KTAFW 225
>TC16731 similar to UP|Q84KJ2 (Q84KJ2) Copper chaperone, partial (83%)
Length = 517
Score = 68.6 bits (166), Expect = 6e-13
Identities = 30/65 (46%), Positives = 47/65 (72%), Gaps = 1/65 (1%)
Frame = +3
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKV-K 173
Q VV++V + C+GC G VKR L K+EGV S++ID++ ++V V G++ P VL+++SK K
Sbjct: 66 QTVVLKVGMSCEGCVGAVKRVLGKLEGVESYDIDLKEQKVVVKGNVQPDTVLQTVSKTGK 245
Query: 174 RAEFW 178
+ FW
Sbjct: 246 KTAFW 260
>BP034759
Length = 358
Score = 65.9 bits (159), Expect = 4e-12
Identities = 29/45 (64%), Positives = 39/45 (86%)
Frame = -1
Query: 134 RHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISKVKRAEFW 178
+HLS+M+GVTSFNID +K+VTV+G ++P+ VL SISKVK A+FW
Sbjct: 358 KHLSRMQGVTSFNIDFAAKKVTVVGDVTPLSVLASISKVKNAQFW 224
>BP049555
Length = 555
Score = 55.8 bits (133), Expect = 4e-09
Identities = 28/106 (26%), Positives = 53/106 (49%)
Frame = +2
Query: 64 SKLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVVVMRVAI 123
+K Q ++ + ++ K +++ + ++E E P+E +K V+ V +
Sbjct: 236 AKQEQPQAEAKPEEKTEEKAEEKAEEKPAAEEKKEEEKPEEEKKEEEPKPPAPCVLYVDL 415
Query: 124 HCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESI 169
HC GCA K++R++ KM GV ID+ VT+ G + P + + I
Sbjct: 416 HCVGCAKKIERYMMKMRGVEGLVIDMAKNEVTIKGIVEPQAICDMI 553
>TC19711
Length = 579
Score = 48.9 bits (115), Expect = 5e-07
Identities = 26/59 (44%), Positives = 36/59 (60%), Gaps = 1/59 (1%)
Frame = +3
Query: 121 VAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESI-SKVKRAEFW 178
V + C GC KVK+ LS + GV S I+ + ++VTV G++ P VL+ S KRAE W
Sbjct: 3 VRMDCDGCELKVKKTLSSLSGVKSVEINRKQQKVTVTGYVEPNKVLKKAKSTGKRAEIW 179
>CB828502
Length = 536
Score = 48.9 bits (115), Expect = 5e-07
Identities = 24/66 (36%), Positives = 41/66 (61%), Gaps = 2/66 (3%)
Frame = +3
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESIS--KV 172
Q V ++V + C GC KV++ + M+GV S +I+ ++ +VTV G++ P V+ I+
Sbjct: 114 QTVEVKVKMDCDGCERKVRKAVEGMKGVNSVDIERKASKVTVTGYVEPNKVVSRIAHHTG 293
Query: 173 KRAEFW 178
K+AE W
Sbjct: 294 KKAEIW 311
>TC13685 similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800), partial
(37%)
Length = 585
Score = 48.5 bits (114), Expect = 7e-07
Identities = 21/65 (32%), Positives = 41/65 (62%), Gaps = 1/65 (1%)
Frame = +2
Query: 111 DNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESIS 170
D+ VV++V +HC+GCA K+ ++L +GV + + + +VTV G + P + +S++
Sbjct: 170 DDTTTTVVLKVDMHCEGCASKIVKNLRAFKGVETVKAESNTGKVTVSGKVDPTKLRDSLA 349
Query: 171 -KVKR 174
K+K+
Sbjct: 350 EKIKK 364
Score = 35.8 bits (81), Expect = 0.004
Identities = 20/86 (23%), Positives = 39/86 (45%), Gaps = 7/86 (8%)
Frame = +2
Query: 64 SKLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQ-------V 116
+KL S + E VS PK ++ + +++ E + +K +
Sbjct: 326 TKLRDSLAEKIKKKVELVSPQPKKEKEKAENKDKDTETNNKAEKKTEEKKINKDKQAVTT 505
Query: 117 VVMRVAIHCQGCAGKVKRHLSKMEGV 142
V++V +HCQGC ++ + + K +GV
Sbjct: 506 AVLKVPLHCQGCIDRIGKFVLKTKGV 583
>BI418928
Length = 496
Score = 47.8 bits (112), Expect = 1e-06
Identities = 18/55 (32%), Positives = 33/55 (59%)
Frame = +3
Query: 117 VVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHISPVVVLESISK 171
VV+++ +HC+GC K+KR L GV D+ ++TV+G + P V + +++
Sbjct: 54 VVLKLDLHCEGCVKKIKRALRNFNGVEDVKADLSGNKLTVIGKVDPTKVRDKLAE 218
Score = 44.7 bits (104), Expect = 1e-05
Identities = 31/99 (31%), Positives = 50/99 (50%), Gaps = 7/99 (7%)
Frame = +3
Query: 55 RLINYANKYSKLVQSR-SRSTSSDRESVSRVPKIKQRNLDQV-EEENEPPKELQ-----K 107
+L NK +LV S + + D+ + P+ K + D+ EEE P E + K
Sbjct: 207 KLAEKTNKNVELVSSPPKKDAAGDKPPPEKKPEEKPKTDDKKPEEEKTKPDEKKAEEKTK 386
Query: 108 TPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFN 146
TP + VVM++ +HC GC K+++ + K +GV S N
Sbjct: 387 TPKQST---VVMKIRLHCDGCIHKIEKIVLKAKGVESVN 494
>TC16918 weakly similar to UP|Q9FJH5 (Q9FJH5) Gb|AAC98457.1 (At5g60800),
partial (24%)
Length = 498
Score = 47.4 bits (111), Expect = 1e-06
Identities = 23/80 (28%), Positives = 42/80 (51%), Gaps = 4/80 (5%)
Frame = +2
Query: 96 EEENEPPKELQKTPTDNVFQ----VVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVES 151
E++ +P E K P + +V ++ +HC+GC K+KR + EGV + ++E+
Sbjct: 65 EKKEQPKNETAKKPDEGAKNDAPAPIVYKLDLHCEGCIKKIKRTVRHFEGVENVKAELEA 244
Query: 152 KRVTVMGHISPVVVLESISK 171
+VTV G V + I++
Sbjct: 245 NKVTVTGKFDAVKLQAKIAE 304
>TC12523
Length = 603
Score = 46.6 bits (109), Expect = 3e-06
Identities = 26/83 (31%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +2
Query: 97 EENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTV 156
EE P E K +Q V++V IHC GC +VK+ L ++GV + ID +V V
Sbjct: 38 EEEAPRGETLK------YQTWVLKVLIHCDGCTKRVKKILQGIDGVYTTEIDPRQHKVIV 199
Query: 157 MGHISPVVVLESISKV-KRAEFW 178
G++ ++ +++ K E W
Sbjct: 200 TGNVDAETLIRRLTRSGKSVELW 268
>BG662295
Length = 382
Score = 44.3 bits (103), Expect = 1e-05
Identities = 18/46 (39%), Positives = 32/46 (69%)
Frame = +1
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVMGHI 160
Q V ++V + C+GC VK + K++G+ S N+++E +RVTV G++
Sbjct: 238 QTVELKVRMCCKGCERVVKNAIYKLKGIDSVNVELEMERVTVTGYV 375
>AV418862
Length = 235
Score = 42.7 bits (99), Expect = 4e-05
Identities = 20/74 (27%), Positives = 42/74 (56%)
Frame = +1
Query: 98 ENEPPKELQKTPTDNVFQVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDVESKRVTVM 157
E P+E ++ P + V++V +HC+ C+ ++KR + +++GV S D+++ V+V
Sbjct: 7 EKPKPEEKKEEPQ---ITIAVLKVHMHCEACSQEIKRRIERIKGVESAEPDLKNSLVSVK 177
Query: 158 GHISPVVVLESISK 171
G ++E + K
Sbjct: 178 GVFETEKLVEYVYK 219
>BP052316
Length = 397
Score = 30.4 bits (67), Expect = 0.19
Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Frame = +2
Query: 115 QVVVMRVAIHCQGCAGKVKRHLSKMEGVTSFNIDV---ESKRVTVMGHISPVVVLESISK 171
Q V++V IHC GC KV+ + S E + + S++VTV G + +++ +++
Sbjct: 191 QTCVLKVNIHCDGCKQKVQAN-SFRELKVFHQVQIACSNSQKVTVSGSVDSATLIKKLTQ 367
>AV408400
Length = 429
Score = 28.5 bits (62), Expect = 0.71
Identities = 16/53 (30%), Positives = 27/53 (50%)
Frame = +3
Query: 65 KLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQKTPTDNVFQVV 117
+++ S S T S RES+S+ K K+ Q + +EP + + + QVV
Sbjct: 36 RIMDSESEDTKSQRESLSKKIKSKKNKKKQDDTSSEPVQANRSETINAKVQVV 194
>TC16644 similar to UP|Q85UH4 (Q85UH4) Cytochrome oxidase subunit III,
partial (7%)
Length = 620
Score = 27.7 bits (60), Expect = 1.2
Identities = 11/27 (40%), Positives = 18/27 (65%)
Frame = -1
Query: 36 IVVVPKRHDRTIFVDDDHTRLINYANK 62
+V PK+HD+ + V DD ++L+ A K
Sbjct: 95 VVT*PKQHDKILRVQDDQSQLLKQAPK 15
>BP035425
Length = 371
Score = 27.3 bits (59), Expect = 1.6
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -1
Query: 161 SPVVVLESISKVKRAEFW 178
+P VVL S+SKVK A+ W
Sbjct: 371 TPSVVLASVSKVKNAQLW 318
>TC12877
Length = 887
Score = 26.9 bits (58), Expect = 2.1
Identities = 11/30 (36%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Frame = +1
Query: 116 VVVMRVAIH--CQGCAGKVKRHLSKMEGVT 143
+ +++ IH C+GC KVK+ L K++ +T
Sbjct: 136 ICILKFNIHFKCEGCEQKVKKLLQKIDVIT 225
>TC10030 similar to UP|Q94JM3 (Q94JM3) AT5g62000/mtg10_20, partial (13%)
Length = 588
Score = 26.6 bits (57), Expect = 2.7
Identities = 20/69 (28%), Positives = 32/69 (45%)
Frame = -3
Query: 47 IFVDDDHTRLINYANKYSKLVQSRSRSTSSDRESVSRVPKIKQRNLDQVEEENEPPKELQ 106
+FV D I++ + V S +T S ES + +QRNL + PPKE+Q
Sbjct: 217 LFVTSDVA--ISHRSSQIPCVSFTSHTTFSSTESA----EAEQRNLRSEIQILNPPKEIQ 56
Query: 107 KTPTDNVFQ 115
+ + + Q
Sbjct: 55 QAQSSTISQ 29
>AV768715
Length = 413
Score = 25.4 bits (54), Expect = 6.0
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = -3
Query: 16 FMCHSRASTAVCMSTRDPRSI 36
F+CH R ++V MS+ DP +I
Sbjct: 81 FICHGR*YSSVMMSSNDPYNI 19
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.129 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,747,013
Number of Sequences: 28460
Number of extensions: 29422
Number of successful extensions: 188
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of query: 180
length of database: 4,897,600
effective HSP length: 84
effective length of query: 96
effective length of database: 2,506,960
effective search space: 240668160
effective search space used: 240668160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0314.12