BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0312.5
(204 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP085186 76 5e-15
AV421890 32 0.077
CB826758 31 0.13
BP037078 29 0.65
TC15689 similar to UP|Q9FJQ2 (Q9FJQ2) Similarity to AP2 domain t... 29 0.65
AV411437 28 0.85
TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-... 28 1.1
TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) prec... 28 1.1
BI417335 28 1.5
TC7822 similar to UP|Q42139 (Q42139) H+-transporting ATP synthas... 27 1.9
BP034655 27 1.9
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 27 2.5
BP033927 26 4.2
BP071245 26 5.5
TC18630 26 5.5
TC9663 similar to UP|Q9FFK7 (Q9FFK7) Signal recognition particle... 26 5.5
TC7925 homologue to UP|RPE_SPIOL (Q43157) Ribulose-phosphate 3-e... 26 5.5
AV406709 26 5.5
TC19183 26 5.5
TC14370 similar to UP|O24375 (O24375) Alpha-glucosidase , parti... 25 7.2
>BP085186
Length = 388
Score = 75.9 bits (185), Expect = 5e-15
Identities = 42/85 (49%), Positives = 53/85 (61%), Gaps = 26/85 (30%)
Frame = -1
Query: 145 AKAKEQISFLNPELAINFKGSDPYARIVDGKLVSPNS----------------------- 181
A+A QISFL+P+LAIN +GS+PYARIV+GKL+SP++
Sbjct: 388 AQALNQISFLHPDLAINLEGSNPYARIVNGKLISPDNADGEGNEEEEEEEEEEEEEEEEE 209
Query: 182 ---AEEEEEKNEEDNGNNKEGEGEN 203
AE EEEKNEE+N N +EGEGEN
Sbjct: 208 EEEAEPEEEKNEEENVNTQEGEGEN 134
>AV421890
Length = 366
Score = 32.0 bits (71), Expect = 0.077
Identities = 14/16 (87%), Positives = 16/16 (99%)
Frame = +1
Query: 15 CQALITKGLEITAISK 30
CQAL+TKGLEI+AISK
Sbjct: 319 CQALLTKGLEISAISK 366
>CB826758
Length = 537
Score = 31.2 bits (69), Expect = 0.13
Identities = 25/78 (32%), Positives = 38/78 (48%), Gaps = 10/78 (12%)
Frame = +2
Query: 74 KKAADLASELNKMKKDFENSLKTKDEEIVNLNTEAVQMK----------SSLESLQAEYS 123
K A L +E ++K E L+T+ EE+ NL ++A ++K SSLES A
Sbjct: 131 KAAESLYAEELNLRKMAEEELETEKEELENLKSQADKVKEELRLVQDQKSSLESQLASSE 310
Query: 124 KLSVESSSLKISVLDHLE 141
+ E IS +D L+
Sbjct: 311 LMVKELEQKIISAVDLLQ 364
>BP037078
Length = 529
Score = 28.9 bits (63), Expect = 0.65
Identities = 22/76 (28%), Positives = 31/76 (39%)
Frame = +1
Query: 83 LNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYSKLSVESSSLKISVLDHLEA 142
L KD LK KDE I S++ESLQ E + L + S + A
Sbjct: 190 LESQIKDKSQELKKKDEVIAEKEKFIQDKSSTVESLQNEVASLQKKGSLDAQEEVGKAHA 369
Query: 143 GFAKAKEQISFLNPEL 158
+ ++Q+ L EL
Sbjct: 370 RAGELQKQVENLKREL 417
>TC15689 similar to UP|Q9FJQ2 (Q9FJQ2) Similarity to AP2 domain
transcription factor, partial (15%)
Length = 872
Score = 28.9 bits (63), Expect = 0.65
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 4/55 (7%)
Frame = -2
Query: 146 KAKEQIS----FLNPELAINFKGSDPYARIVDGKLVSPNSAEEEEEKNEEDNGNN 196
KA + IS F NP+ F S + K V+P+S EEE+ ++ D G+N
Sbjct: 514 KAAQSISKEGYFSNPK----FSTSSHWFESEKSKKVTPDSGEEEQGEDSSDRGDN 362
>AV411437
Length = 417
Score = 28.5 bits (62), Expect = 0.85
Identities = 15/38 (39%), Positives = 19/38 (49%)
Frame = +2
Query: 165 SDPYARIVDGKLVSPNSAEEEEEKNEEDNGNNKEGEGE 202
S P V G AEEEEE+ EE+ + +GE E
Sbjct: 2 SPPPLAAVSGSSAQETIAEEEEEEEEEEEEDGVDGELE 115
>TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-phosphate
reductase, partial (54%)
Length = 547
Score = 28.1 bits (61), Expect = 1.1
Identities = 11/16 (68%), Positives = 14/16 (86%)
Frame = +3
Query: 183 EEEEEKNEEDNGNNKE 198
EEEEE+ EE+NGN+ E
Sbjct: 18 EEEEEEEEEENGNHPE 65
>TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) precursor,
partial (51%)
Length = 1566
Score = 28.1 bits (61), Expect = 1.1
Identities = 17/65 (26%), Positives = 33/65 (50%), Gaps = 7/65 (10%)
Frame = +1
Query: 142 AGF-AKAKEQISFLNPELAINFKGSDPYARIV------DGKLVSPNSAEEEEEKNEEDNG 194
+GF A+ +Q+ ++ + A + D R + D +SP SA+E+E++ +ED
Sbjct: 361 SGFGAEFLQQVFNIDHDTAKQLQSPDDQRRQIVKVEGDDLSFISPESADEDEDEEDEDED 540
Query: 195 NNKEG 199
+ G
Sbjct: 541 QEQHG 555
>BI417335
Length = 491
Score = 27.7 bits (60), Expect = 1.5
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = -3
Query: 182 AEEEEEKNEEDNGNNKEGEGE 202
A++EEE EED+ + ++GEGE
Sbjct: 87 AQDEEEDEEEDSFSAEQGEGE 25
>TC7822 similar to UP|Q42139 (Q42139) H+-transporting ATP synthase CHAIN9 -
like protein (AT4G32260/F10M6_100) , partial (76%)
Length = 907
Score = 27.3 bits (59), Expect = 1.9
Identities = 25/99 (25%), Positives = 53/99 (53%), Gaps = 9/99 (9%)
Frame = +3
Query: 46 KQLEEKDKEITKLKATMKLLSNSNKTNEKKA--------ADLASELNKMKKDFENSLKTK 97
K ++++D I + ++K S+ K E++A A++++ LNKMKK+ + ++ K
Sbjct: 426 KFMDDRDAAIREKLNSVKDTSSEVKQLEEQANAVLKAARAEISAALNKMKKETQVEVEQK 605
Query: 98 -DEEIVNLNTEAVQMKSSLESLQAEYSKLSVESSSLKIS 135
E + ++ E + ++LE + E K S++S +S
Sbjct: 606 IAEGRLKVDAELQEALANLEKQKEETIK-SLDSQIAALS 719
>BP034655
Length = 517
Score = 27.3 bits (59), Expect = 1.9
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +1
Query: 183 EEEEEKNEEDNGNNKEGEGENH 204
EEEEE+ EED +++E E E++
Sbjct: 193 EEEEEEEEEDEEDDEEDEDEDY 258
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 26.9 bits (58), Expect = 2.5
Identities = 9/21 (42%), Positives = 19/21 (89%)
Frame = +2
Query: 183 EEEEEKNEEDNGNNKEGEGEN 203
+EEEE +++D+ +++EGEG++
Sbjct: 824 DEEEEDDDDDDEDDEEGEGKS 886
>BP033927
Length = 493
Score = 26.2 bits (56), Expect = 4.2
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = +2
Query: 177 VSPNSAEEEEEKNEEDNGNNKEGEGE 202
VSP+ +E ++ K + + + EGEGE
Sbjct: 404 VSPDQSESKKRKRKREGEGDGEGEGE 481
>BP071245
Length = 552
Score = 25.8 bits (55), Expect = 5.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +1
Query: 169 ARIVDGKLVSPNSAEEEEEKNEEDNGNNKE 198
+ ++D L+ PN+ E ++E +N NK+
Sbjct: 295 SNLMDEMLIPPNAKRGRESESESENEENKD 384
>TC18630
Length = 351
Score = 25.8 bits (55), Expect = 5.5
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -3
Query: 183 EEEEEKNEEDNGNNKEGEGE 202
E+E EED N+EGEGE
Sbjct: 160 EKEGRVREEDMAENEEGEGE 101
>TC9663 similar to UP|Q9FFK7 (Q9FFK7) Signal recognition particle
receptor beta subunit-like protein, partial (44%)
Length = 612
Score = 25.8 bits (55), Expect = 5.5
Identities = 16/58 (27%), Positives = 32/58 (54%), Gaps = 2/58 (3%)
Frame = +1
Query: 18 LITKGLEITAISKMIDLESNVFDGTDNAKQL--EEKDKEITKLKATMKLLSNSNKTNE 73
++TKG + ++ L + T + K+ + +KEI KL+A+ +S+++ TNE
Sbjct: 37 ILTKGSVVKKKIPLLILCNKTDKVTAHTKEFIRRQLEKEIDKLRASRSAVSDADVTNE 210
>TC7925 homologue to UP|RPE_SPIOL (Q43157) Ribulose-phosphate 3-epimerase,
chloroplast precursor (Pentose-5-phosphate 3-epimerase)
(PPE) (RPE) (R5P3E) , partial (94%)
Length = 1387
Score = 25.8 bits (55), Expect = 5.5
Identities = 29/112 (25%), Positives = 46/112 (40%), Gaps = 1/112 (0%)
Frame = +3
Query: 70 KTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYSKLSVES 129
K NE A L S + + + +SL +K N + + ++ S++ S
Sbjct: 135 KQNEMAATSLCSSTLQSQINGPSSLHSKTAIFNNHPRSLTFSRRKISTIVKASSRVDKFS 314
Query: 130 SSLKISVLDHLEAGFAKAKEQISFLNPELAINFKGSD-PYARIVDGKLVSPN 180
S I L A FAK EQ+ A+ G D + ++DG+ V PN
Sbjct: 315 KSDIIVSPSILSANFAKLGEQVK------AVELAGCDWIHVDVMDGRFV-PN 449
>AV406709
Length = 430
Score = 25.8 bits (55), Expect = 5.5
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1
Query: 181 SAEEEEEKNEEDNGNNKEGE 200
S E EEE+ EED N +E E
Sbjct: 145 SPEREEEEEEEDGSNGEEEE 86
>TC19183
Length = 573
Score = 25.8 bits (55), Expect = 5.5
Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 1/74 (1%)
Frame = +2
Query: 64 LLSNSNKTNEKKAADLASELNKMK-KDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEY 122
L S S+ T+ ++ + + K K + + ++ + EI E+ + + E L+ E
Sbjct: 44 LQSRSSMTDPLRSVKVXPDPIKAKVTELKMKIENINSEIGEREQESSVLDEAREKLKREL 223
Query: 123 SKLSVESSSLKISV 136
ESS LKI V
Sbjct: 224 DHYDAESSKLKIQV 265
>TC14370 similar to UP|O24375 (O24375) Alpha-glucosidase , partial (9%)
Length = 837
Score = 25.4 bits (54), Expect = 7.2
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = -2
Query: 144 FAKAKEQISFLNPELAINFKGSDPYARIVDGKLVSPN 180
F K +SFL L +NFK + +I G L N
Sbjct: 497 FFKTSASLSFLRQHLRVNFKSTQLQTKISFGTLSYKN 387
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.303 0.123 0.311
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,241,658
Number of Sequences: 28460
Number of extensions: 20551
Number of successful extensions: 149
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of query: 204
length of database: 4,897,600
effective HSP length: 86
effective length of query: 118
effective length of database: 2,450,040
effective search space: 289104720
effective search space used: 289104720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.8 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0312.5