BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0306.8
(641 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8045 weakly similar to UP|ST35_ARATH (Q94LW6) Probable sulfate... 291 3e-79
CB829305 197 6e-51
TC8880 similar to UP|ST35_ARATH (Q94LW6) Probable sulfate transp... 178 3e-45
TC11476 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate ... 177 5e-45
TC17837 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate ... 157 5e-39
AW720566 44 8e-05
BP037671 39 0.003
TC12806 UP|O81120 (O81120) Nodule-specific protein Nlj70, complete 33 0.11
BP064580 33 0.14
TC16114 similar to UP|AAQ62433 (AAQ62433) At3g01800, partial (73%) 31 0.70
TC9557 30 0.91
TC16063 30 1.6
AV777506 28 3.5
TC11062 similar to UP|O64438 (O64438) ARG10, complete 28 5.9
BP069929 27 7.7
>TC8045 weakly similar to UP|ST35_ARATH (Q94LW6) Probable sulfate
transporter 3.5, partial (39%)
Length = 1616
Score = 291 bits (744), Expect = 3e-79
Identities = 149/388 (38%), Positives = 247/388 (63%), Gaps = 2/388 (0%)
Frame = +3
Query: 250 ILGSSFLIFILTTRFLGKKKKKLFWLASIAPLVSIILSTLIVFLTRADKSGVKIVKHVKG 309
+LG FL F+ TR + K+ KLFW+++IAP+ +I+ ++ V+L K G+ IV H+
Sbjct: 15 VLGLVFLAFLQFTRHVRNKRPKLFWVSAIAPMTVVIVGSIFVYLVHGQKHGIPIVGHLDR 194
Query: 310 GLNPSSLHELDFNNPHVGEAAKIGLVVAVVALTESIAVGRSFASIKGYQLDGNKEMMSIG 369
GLNP S+ +F++ ++ + L+ V++L E IA+GRSF+ DGNKEM++ G
Sbjct: 195 GLNPWSIQYFNFDSKYLPAVMQAALITGVLSLAEGIAIGRSFSVTDNTPHDGNKEMVAFG 374
Query: 370 FSNIIGSLTSCYVATGSFSRTAVNYAAGCETLISNIVMAITVLISLQFFTKLLYYTPTAI 429
N+ GS TSCY+ +G FS+TAVNY AG +T ++N+V A+ + ++LQF L +TP
Sbjct: 375 LMNLFGSFTSCYLTSGPFSKTAVNYNAGGKTAMTNVVQAVLMALTLQFLAPLFGFTPLVA 554
Query: 430 IASVILSALPGLIDIPEACKIWKVDKLDFLACAGAFFGVLFASVEIGLLAAVMISFLKII 489
++++I SA+ GL++ E ++KVDK DF+ C AF GV F ++ GL+ +V + ++ +
Sbjct: 555 LSAIITSAMLGLVNYTEVIYLYKVDKFDFVICMAAFLGVAFLGMDYGLMISVGLGVIRAL 734
Query: 490 LISIRPDTEALGKLPGTSLFCDVYQYPMAVQIPGVVVIRVKSALLCFANANFVRERIMKW 549
L RP T LGKL ++ DV QYP A PG++++++ S + F+N+ +V+ER+M++
Sbjct: 735 LYVARPATCKLGKLNEFGIYRDVEQYP-ASTFPGLIIVQLGSPVY-FSNSVYVKERVMRY 908
Query: 550 V-TQEESKDDKGNSTNTIQLVILETSNLVDIDTSGIASLEEMQKVLISNGKQLAIANPRW 608
+ +Q+ S +D ++ VIL+ S + IDT+ I L E+ K+L NG ++ + NPR
Sbjct: 909 IKSQQRSNED------VVEQVILDMSGVTSIDTTAIEGLLELNKMLEKNGIEMFLVNPRL 1070
Query: 609 QVIHKLKVSNFVSKIGGR-IYLTVEEAI 635
+V+ KL +S FV K+G YLT+++A+
Sbjct: 1071EVMEKLIISKFVDKLGKESFYLTLDDAV 1154
>CB829305
Length = 509
Score = 197 bits (500), Expect = 6e-51
Identities = 97/162 (59%), Positives = 124/162 (75%)
Frame = +3
Query: 329 AAKIGLVVAVVALTESIAVGRSFASIKGYQLDGNKEMMSIGFSNIIGSLTSCYVATGSFS 388
A K GLV +++LTE IAVGR+FA+++ YQ+DGNKEM++IG NI GS +SCYV TGSFS
Sbjct: 12 AIKTGLVTGILSLTEGIAVGRTFAALRNYQVDGNKEMIAIGVMNIAGSCSSCYVTTGSFS 191
Query: 389 RTAVNYAAGCETLISNIVMAITVLISLQFFTKLLYYTPTAIIASVILSALPGLIDIPEAC 448
R+AVNY AG T +SNI+MA VL++L F L YYTP ++A++I++A+ GLID A
Sbjct: 192 RSAVNYNAGARTAVSNIIMASAVLVTLLFLMPLFYYTPNVVLAAIIITAVIGLIDYQAAY 371
Query: 449 KIWKVDKLDFLACAGAFFGVLFASVEIGLLAAVMISFLKIIL 490
K+WKVDKLDFLAC +FFGVLF SV +GL AV IS KI+L
Sbjct: 372 KLWKVDKLDFLACLCSFFGVLFISVPLGLGIAVAISVFKILL 497
>TC8880 similar to UP|ST35_ARATH (Q94LW6) Probable sulfate transporter 3.5,
partial (30%)
Length = 794
Score = 178 bits (451), Expect = 3e-45
Identities = 84/173 (48%), Positives = 111/173 (63%)
Frame = +3
Query: 64 LQGIFPILSWGRNYTAAKFRKDILAGLTIASLCIPQSIGYATLAHLDPQYGLYTSVVPPL 123
+Q PI W NYT F D ++GLTI SL IPQ I YA LA+L P GLY+S VPPL
Sbjct: 258 VQYYVPIFEWLPNYTLRLFISDFISGLTITSLAIPQGISYAKLANLPPIVGLYSSFVPPL 437
Query: 124 IYAVMGTSREVAVGPVAVVSLLLFSMVQKLVDPAVDPVAYTKLVFLTTLFAGIFQTAFGL 183
+YA+ G+SR +AVG +A SLL+ + + P DP Y L+F TT G+FQ G+
Sbjct: 438 VYAIFGSSRHMAVGTLAAASLLIGQTISTVASPETDPTLYLHLIFTTTFVTGVFQACLGI 617
Query: 184 FRLGFLVDFLSHAAIVGFVAGAAIVIGLQQFKGLLGITHFTTKTDIISVMKAV 236
FRLG LVDF SH+ I GF+ G A ++ QQ KG G+ HF+TKT+++ V K++
Sbjct: 618 FRLGILVDFFSHSTITGFMGGTAFILIAQQLKGFFGMKHFSTKTNLVEVAKSI 776
>TC11476 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate transporter
3, partial (18%)
Length = 597
Score = 177 bits (449), Expect = 5e-45
Identities = 93/185 (50%), Positives = 123/185 (66%), Gaps = 3/185 (1%)
Frame = +1
Query: 7 LDIEKNTQEVRSQWVLNAPEPPTAWNMVTDSVKKTISQFPRKLSYLKDQP---CNTLMSF 63
L +E +Q RSQWVL++P PP W + +SVKKTI + +S
Sbjct: 37 LHLENASQPGRSQWVLDSPNPPPLWKKLFNSVKKTILPHGNNRFCFSSKNRTFWGHALSL 216
Query: 64 LQGIFPILSWGRNYTAAKFRKDILAGLTIASLCIPQSIGYATLAHLDPQYGLYTSVVPPL 123
LQ +FPIL+W R+YT +KF+ D+LAGLT+ASLCIPQSIGYA LA +DP+YGLYTS+VPPL
Sbjct: 217 LQSLFPILTWIRDYTFSKFKDDLLAGLTLASLCIPQSIGYAHLAQVDPEYGLYTSIVPPL 396
Query: 124 IYAVMGTSREVAVGPVAVVSLLLFSMVQKLVDPAVDPVAYTKLVFLTTLFAGIFQTAFGL 183
IY +MG+SRE+A+GP AVVS+LL S+VQK+ DP +P + K L F +
Sbjct: 397 IYPLMGSSREIAIGPAAVVSMLLSSLVQKVEDPIANP*SL*KSNLHCDLLLWNFSSCIWH 576
Query: 184 FRLGF 188
F++GF
Sbjct: 577 FQVGF 591
Score = 46.6 bits (109), Expect = 1e-05
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = +2
Query: 160 PVAYTKLVFLTTLFAGIFQTAFGLFRLGFL 189
P AY L+F T F+GIFQ AFG+FRLGFL
Sbjct: 506 PEAYRNLIFTVTFFSGIFQAAFGIFRLGFL 595
>TC17837 similar to UP|SUT3_STYHA (P53393) Low affinity sulphate transporter
3, partial (16%)
Length = 466
Score = 157 bits (397), Expect = 5e-39
Identities = 74/102 (72%), Positives = 93/102 (90%)
Frame = +1
Query: 286 LSTLIVFLTRADKSGVKIVKHVKGGLNPSSLHELDFNNPHVGEAAKIGLVVAVVALTESI 345
LSTLIV+L++ADK GV I+KHVKGGLNPSS+H+L F++P+VG+AAKIGL+ A++ LTE+I
Sbjct: 1 LSTLIVYLSKADKIGVNILKHVKGGLNPSSVHQLQFHDPNVGQAAKIGLICAIIGLTEAI 180
Query: 346 AVGRSFASIKGYQLDGNKEMMSIGFSNIIGSLTSCYVATGSF 387
AVGRSFASI+GY LDGN+EM+S+G +NI GSLTSCYVATG F
Sbjct: 181 AVGRSFASIQGYHLDGNEEMLSMGVTNIAGSLTSCYVATGKF 306
>AW720566
Length = 549
Score = 43.9 bits (102), Expect = 8e-05
Identities = 20/45 (44%), Positives = 30/45 (66%)
Frame = +2
Query: 580 DTSGIASLEEMQKVLISNGKQLAIANPRWQVIHKLKVSNFVSKIG 624
DT+ I L E+ K+L NG ++ + NPR +V+ KL +S FV K+G
Sbjct: 407 DTTAIEGLLELNKMLEKNGIEMFLVNPRLEVMEKLIISKFVDKLG 541
>BP037671
Length = 468
Score = 38.9 bits (89), Expect = 0.003
Identities = 21/59 (35%), Positives = 35/59 (58%), Gaps = 2/59 (3%)
Frame = -3
Query: 579 IDTSGIASLEEMQKVLISNGKQLAIANPRWQVIHKLKVSNFVSKI--GGRIYLTVEEAI 635
IDTSGI+ +E + + G +L + NP +VI KLK ++ ++ ++LTV EA+
Sbjct: 460 IDTSGISFFKEFKSTMEKKGVELVLVNPLAEVIEKLKKADEANEFIREDNLFLTVGEAV 284
>TC12806 UP|O81120 (O81120) Nodule-specific protein Nlj70, complete
Length = 2069
Score = 33.5 bits (75), Expect = 0.11
Identities = 19/56 (33%), Positives = 32/56 (56%), Gaps = 1/56 (1%)
Frame = +1
Query: 407 MAITVLISLQF-FTKLLYYTPTAIIASVILSALPGLIDIPEACKIWKVDKLDFLAC 461
+ I +++ + F FT+ YY T ++ ++L LP + I E CKIWK K + + C
Sbjct: 748 LMIMIILQISFNFTQSEYYATTTVM--LLLLTLPLAVVIVEDCKIWK-SKQELINC 906
>BP064580
Length = 538
Score = 33.1 bits (74), Expect = 0.14
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +3
Query: 268 KKKKLFWLASIAPLVSIILSTL 289
KK KLFW+++ PL S+ILST+
Sbjct: 471 KKPKLFWVSAAXPLTSVILSTI 536
>TC16114 similar to UP|AAQ62433 (AAQ62433) At3g01800, partial (73%)
Length = 1297
Score = 30.8 bits (68), Expect = 0.70
Identities = 30/87 (34%), Positives = 44/87 (50%), Gaps = 3/87 (3%)
Frame = +3
Query: 510 CDVYQYPMAVQIPGVVVIRVKSALLCFANANFVRERIMKWVTQEESKDDKGNSTNTIQLV 569
CDV P A+QI + RV + A ANF+ E + +SKDD G ST + LV
Sbjct: 219 CDV---PGAIQIRALESNRVSTLP---AAANFIVESRRAFAKGRKSKDDAGMSTIEVPLV 380
Query: 570 ILET--SNLVDIDTSGIASLE-EMQKV 593
+ T +N V + I++L E+ K+
Sbjct: 381 LGPTIKANAVSQMEAAISALSTELSKL 461
>TC9557
Length = 538
Score = 30.4 bits (67), Expect = 0.91
Identities = 14/32 (43%), Positives = 16/32 (49%)
Frame = +1
Query: 444 IPEACKIWKVDKLDFLACAGAFFGVLFASVEI 475
IP+ C I KV FL C G FF + S I
Sbjct: 400 IPDCCNI*KVRLTTFLICPGLFFSCIIVSCNI 495
>TC16063
Length = 585
Score = 29.6 bits (65), Expect = 1.6
Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = -2
Query: 362 NKEMMSIGFSNIIGSLTSCYVA-TGSFSRTAVNYAAGCETLISNIVMAITVLISLQFFTK 420
N + G +N G++ +C + +G FS V+ AGC+ + + + V + FF++
Sbjct: 557 NTRVSPFGLAN--GTVKTCLLKPSGDFSHQTVSTVAGCQLSAATALYS*AVYVMNHFFSR 384
Query: 421 LLY 423
+ Y
Sbjct: 383 ISY 375
>AV777506
Length = 449
Score = 28.5 bits (62), Expect = 3.5
Identities = 18/53 (33%), Positives = 24/53 (44%), Gaps = 2/53 (3%)
Frame = +1
Query: 160 PVAYTKLVFLTTLFAGIFQTAFGLFRLGFLVDFLSHAAIVG--FVAGAAIVIG 210
P YT L + +F F + RLGF+ FLS+ V F+ I IG
Sbjct: 139 PHHYTLLFISVLVLCPLFPPVFIILRLGFMPSFLSNLFSVSPFFILSLGISIG 297
>TC11062 similar to UP|O64438 (O64438) ARG10, complete
Length = 1032
Score = 27.7 bits (60), Expect = 5.9
Identities = 20/69 (28%), Positives = 32/69 (45%), Gaps = 1/69 (1%)
Frame = +2
Query: 242 NPWQPRNF-ILGSSFLIFILTTRFLGKKKKKLFWLASIAPLVSIILSTLIVFLTRADKSG 300
+PWQPR+F + F IF LG +++ + LA A V +++ RA
Sbjct: 275 SPWQPRSFAVKDEIFCIFEGALDNLGSLRQQ-YGLAKSANEVLLVIEAYKALRDRAPYPA 451
Query: 301 VKIVKHVKG 309
+V H+ G
Sbjct: 452 NHVVGHLSG 478
>BP069929
Length = 421
Score = 27.3 bits (59), Expect = 7.7
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = +1
Query: 563 TNTIQLVILETSNLVDIDTSGIASLEEMQKVLISNGKQLAIANPRWQVIHKLKVSNFVSK 622
T Q + SNL + TS +M+ + N K + +PR QV+H + SNF+ +
Sbjct: 52 TTKSQTMYRYMSNLFNSITS------KMEAKVTKNIKGCPLHDPRSQVLHNIDTSNFLKR 213
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.139 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,087,469
Number of Sequences: 28460
Number of extensions: 124020
Number of successful extensions: 797
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 794
length of query: 641
length of database: 4,897,600
effective HSP length: 96
effective length of query: 545
effective length of database: 2,165,440
effective search space: 1180164800
effective search space used: 1180164800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0306.8