BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0306.4
(968 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU089582 105 2e-36
AV427422 130 8e-31
BP055130 96 8e-21
BG662087 94 1e-19
BF177840 94 1e-19
BP054462 74 1e-13
AV410603 72 5e-13
BE122516 54 1e-07
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 50 2e-06
TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%) 50 2e-06
BP046863 46 3e-05
TC18698 39 0.004
TC12574 36 0.033
BP064003 33 0.28
BP048192 31 0.82
BE122510 28 5.3
BP043519 28 5.3
TC13072 similar to UP|Q9FR53 (Q9FR53) PTOR, partial (8%) 28 7.0
TC9644 homologue to UP|Q9M394 (Q9M394) Fructokinase-like protein... 28 7.0
TC18086 similar to UP|O81023 (O81023) At2g26840 protein, partial... 28 9.1
>AU089582
Length = 383
Score = 105 bits (261), Expect(2) = 2e-36
Identities = 49/76 (64%), Positives = 61/76 (79%)
Frame = +2
Query: 657 AEIYVAEIVRLHGVSTSIVSDSDPKFTSHFWGALHDALGTKLRLSSAYHPQIDGQTERTI 716
A+IY+ EIV LHGV SI+SD +FTSHFW + ALGT+L++S+A+HPQ DGQ+ERTI
Sbjct: 23 AKIYLDEIVSLHGVPVSIISDRGAQFTSHFWRSFQTALGTRLKMSTAFHPQTDGQSERTI 202
Query: 717 QSLEDLLRACVLDNRG 732
Q LED+LRACV D RG
Sbjct: 203 QILEDMLRACVXDLRG 250
Score = 65.9 bits (159), Expect(2) = 2e-36
Identities = 26/45 (57%), Positives = 36/45 (79%)
Frame = +3
Query: 732 GSWDDLQPLIEFTYNNNFHASIGMAPYEALYGRKCRTPLCWYQDG 776
GSWD L+EF YNN++ +SI MAP+EALYGR+CR+P+ W++ G
Sbjct: 249 GSWDQYLSLMEFAYNNSYRSSI*MAPFEALYGRRCRSPIGWFEVG 383
>AV427422
Length = 417
Score = 130 bits (328), Expect = 8e-31
Identities = 64/137 (46%), Positives = 90/137 (64%)
Frame = +1
Query: 621 LPRTQKRHDSIWVIVDRLTKSAHFLPGRTTYNVEKLAEIYVAEIVRLHGVSTSIVSDSDP 680
LP++ K ++++ V+VDRL+K +HF+P + Y + +A+I+V E+VRLHGV SIVSD DP
Sbjct: 10 LPKS-KGYEAVLVVVDRLSKFSHFVPLKHPYTAKVIADIFVREVVRLHGVPLSIVSDRDP 186
Query: 681 KFTSHFWGALHDALGTKLRLSSAYHPQIDGQTERTIQSLEDLLRACVLDNRGSWDDLQPL 740
F S+FW L GTKL++S+AYHP+ DGQTE + LE LR + D SW P
Sbjct: 187 LFMSNFWKELFKMQGTKLKMSTAYHPESDGQTEVVNRCLETYLRCFIADQPKSWAHWVPW 366
Query: 741 IEFTYNNNFHASIGMAP 757
E+ YN ++H S G P
Sbjct: 367 AEYWYNTSYHVSTGQTP 417
>BP055130
Length = 567
Score = 95.9 bits (237), Expect(2) = 8e-21
Identities = 50/104 (48%), Positives = 63/104 (60%)
Frame = +2
Query: 631 IWVIVDRLTKSAHFLPGRTTYNVEKLAEIYVAEIVRLHGVSTSIVSDSDPKFTSHFWGAL 690
I V+VDRL+K HF P R Y ++AE +V+ IV+LHG+ +IVSD D FTS FW
Sbjct: 191 IIVVVDRLSKYGHFAPHRANYTSSQVAETFVSTIVKLHGMPRAIVSDRDKAFTSAFWKHF 370
Query: 691 HDALGTKLRLSSAYHPQIDGQTERTIQSLEDLLRACVLDNRGSW 734
GT L +SS+YHPQ DGQTE + LE LR V + W
Sbjct: 371 FKLHGTTLNMSSSYHPQTDGQTEALNKCLELYLRCFVHETPRLW 502
Score = 22.3 bits (46), Expect(2) = 8e-21
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = +3
Query: 739 PLIEFTYNNNFHASIG 754
P E+ YN++F SIG
Sbjct: 519 PWAEYCYNSSFQTSIG 566
>BG662087
Length = 373
Score = 94.0 bits (232), Expect = 1e-19
Identities = 47/113 (41%), Positives = 70/113 (61%)
Frame = +1
Query: 81 GKSRLCVDYRQLNKATVKNRYPLPRIDDLMDQLRGDAVFSKIDLKSSYHQIRVKTEDIPK 140
GK R+ VDY LNKA K+ YPLP ID L+D + + S +D S YHQI++ D K
Sbjct: 16 GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195
Query: 141 TAFRTRYGHYEYLVMPFGVTNAPAVFMDYMNRIFHEFLDRFVVVFIDDILIYS 193
TAF T +Y Y +PFG+ NA A + M+R+F + + R + V++D++++ S
Sbjct: 196 TAFMTARVNYCYQTIPFGLKNAGATYQXLMDRVFXDXVGRNMEVYLDNMIVKS 354
>BF177840
Length = 410
Score = 94.0 bits (232), Expect = 1e-19
Identities = 53/130 (40%), Positives = 73/130 (55%)
Frame = +2
Query: 672 TSIVSDSDPKFTSHFWGALHDALGTKLRLSSAYHPQIDGQTERTIQSLEDLLRACVLDNR 731
TSIVSD D KF SHFW L +GTKL S+ HPQ DGQTE ++L LLR+ + N
Sbjct: 11 TSIVSDRDTKFISHFWRTLWGKVGTKLLYSTTCHPQTDGQTEVVNKTLSTLLRSVLERNL 190
Query: 732 GSWDDLQPLIEFTYNNNFHASIGMAPYEALYGRKCRTPLCWYQDGESLLIGPELLQQTTE 791
W+ P IEF YN H++ +P+E +YG TPL + L+ + + E
Sbjct: 191 KMWETWLPHIEFAYNRVVHSTTKHSPFEIVYGYNPLTPLDLLPMPNTYLLKHKDGKAKAE 370
Query: 792 KVKQIREKMR 801
VK++ EK++
Sbjct: 371 FVKRLHEKIK 400
>BP054462
Length = 422
Score = 73.9 bits (180), Expect = 1e-13
Identities = 40/125 (32%), Positives = 71/125 (56%), Gaps = 1/125 (0%)
Frame = +1
Query: 746 NNNFHASIGMAPYEALYGRKCRTPLCWYQDGESLLIGPELLQQTTEKVKQIREKMRASQS 805
N+N++ S M+P++ALYGR+ L + +L E + +R + SQ
Sbjct: 1 NSNYNRSAKMSPFQALYGREPPVLLQGTTIPSKIAAVNDLQVGRDELLSDLRANLLKSQD 180
Query: 806 RQKSYADQRRRTLEFEEGDHVFLRVTQTTGVGRAIK-SRKLTPKFIGPYQITRRVGPVAY 864
++YA+++RR ++++ GD VFL++ A K + KL+P++ GPY I ++G VAY
Sbjct: 181 MMRTYANKKRRDVDYQIGDEVFLKLQPYRRRSLAKKMNEKLSPRYYGPYPIVAKIGAVAY 360
Query: 865 QIALP 869
++ LP
Sbjct: 361 RLELP 375
>AV410603
Length = 162
Score = 71.6 bits (174), Expect = 5e-13
Identities = 31/54 (57%), Positives = 42/54 (77%)
Frame = +1
Query: 95 ATVKNRYPLPRIDDLMDQLRGDAVFSKIDLKSSYHQIRVKTEDIPKTAFRTRYG 148
+TVK+ +P+P +D+L+D+LRG FSK+DL+S YHQI VK ED KT FRT +G
Sbjct: 1 STVKDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTHHG 162
>BE122516
Length = 364
Score = 53.9 bits (128), Expect = 1e-07
Identities = 28/46 (60%), Positives = 32/46 (68%)
Frame = +2
Query: 1 IRDFC*CCSEEVPRLPPVRDVEFVIDIVRGFGPISIAPYRMAPAEL 46
+R+F EEV LPP R+VEF ID V G GPISIAPYRM+ EL
Sbjct: 215 VREFSDVFPEEVSELPPEREVEFSID*VPGTGPISIAPYRMSLVEL 352
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 49.7 bits (117), Expect = 2e-06
Identities = 27/90 (30%), Positives = 45/90 (50%)
Frame = +2
Query: 681 KFTSHFWGALHDALGTKLRLSSAYHPQIDGQTERTIQSLEDLLRACVLDNRGSWDDLQPL 740
+FTS D +G ++R SS HPQ +GQTE + + ++ + + G W D P+
Sbjct: 20 QFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYEAEGRWIDELPI 199
Query: 741 IEFTYNNNFHASIGMAPYEALYGRKCRTPL 770
+ ++YN +SI P+ YG P+
Sbjct: 200 VLWSYNTMPQSSIKETPF*LTYGADTMLPV 289
>TC19412 similar to UP|Q84KB0 (Q84KB0) Pol protein, partial (7%)
Length = 519
Score = 49.7 bits (117), Expect = 2e-06
Identities = 25/51 (49%), Positives = 35/51 (68%), Gaps = 4/51 (7%)
Frame = +3
Query: 829 RVTQTTGVGRAIKSRKLTPKFIGPYQITRRVGPVAYQIALPPFL----PIF 875
RV+ GV R K KL+P+FIGP+++ RVG V+Y++ALPP L P+F
Sbjct: 3 RVSPMKGVLRFGKKGKLSPRFIGPFEVLERVGSVSYRLALPPDLSAVHPVF 155
>BP046863
Length = 580
Score = 45.8 bits (107), Expect = 3e-05
Identities = 23/81 (28%), Positives = 50/81 (61%), Gaps = 1/81 (1%)
Frame = +1
Query: 785 LLQQTTEKVKQIREKMRASQSRQKSYADQRRRTLEFEEGDHVFLRVTQTTGVGRAI-KSR 843
L+++ + ++R + +Q + ++ A++ RR ++++ G+ VFL++ A K++
Sbjct: 274 LIEERDALLLELRGNLLKAQDQMRAQANKHRRYVDYQVGNWVFLKLQPYKLQNLAQRKNQ 453
Query: 844 KLTPKFIGPYQITRRVGPVAY 864
KL+P+F GP+++ RV VAY
Sbjct: 454 KLSPRFYGPFKVLERVVQVAY 516
>TC18698
Length = 808
Score = 38.9 bits (89), Expect = 0.004
Identities = 17/54 (31%), Positives = 32/54 (58%)
Frame = -2
Query: 140 KTAFRTRYGHYEYLVMPFGVTNAPAVFMDYMNRIFHEFLDRFVVVFIDDILIYS 193
KT + +Y Y VMP G+ N + M++IFH+ + + V V+++D+++ S
Sbjct: 801 KTTLKINRVNYYYQVMPLGLKNI*TTYQRLMDKIFHKQI*KNVEVYVEDMIVKS 640
>TC12574
Length = 325
Score = 35.8 bits (81), Expect = 0.033
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = +2
Query: 166 FMDYMNRIFHEFLDRFVVVFIDDILIYS*DTKE 198
F + +N IF F + F++VFI+DIL Y+ D +E
Sbjct: 2 FKNSVNHIFESFFEHFMIVFINDILSYTEDKEE 100
>BP064003
Length = 509
Score = 32.7 bits (73), Expect = 0.28
Identities = 22/78 (28%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Frame = +1
Query: 388 GCTFTIFSDHKSLKYLFDQKELNMRQRRWMEFIKD---YEFTLQYHPGKANVVADALSRK 444
G F I +D+ + QK R R W +F+ + +Q ++N VADALSRK
Sbjct: 115 GSRFVIKTDNVATSSFLTQKRAPTRAR-WQDFLSGGVRHGPRVQAREDQSNKVADALSRK 291
Query: 445 MHVSLKMVKELELLEQFR 462
++ ++E+ + Q +
Sbjct: 292 AELASNKLEEIADMSQLK 345
>BP048192
Length = 551
Score = 31.2 bits (69), Expect = 0.82
Identities = 12/37 (32%), Positives = 23/37 (61%), Gaps = 3/37 (8%)
Frame = +3
Query: 391 FTIFSDHKSLKYLFDQ---KELNMRQRRWMEFIKDYE 424
F ++D+ ++ Y Q KE+++ + RW +IKDY+
Sbjct: 198 FLTYADNNAVGYFIKQGFTKEIHLEKERWQGYIKDYD 308
>BE122510
Length = 385
Score = 28.5 bits (62), Expect = 5.3
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = -2
Query: 573 KKQVVEYVAACLTCQKAKVE 592
+K+ EYV+ LTCQKAKVE
Sbjct: 384 EKE*SEYVSTRLTCQKAKVE 325
>BP043519
Length = 456
Score = 28.5 bits (62), Expect = 5.3
Identities = 19/59 (32%), Positives = 26/59 (43%)
Frame = +3
Query: 101 YPLPRIDDLMDQLRGDAVFSKIDLKSSYHQIRVKTEDIPKTAFRTRYGHYEYLVMPFGV 159
Y LP + LMD+ G D Y + + K AFR +YG + VMPF +
Sbjct: 237 YALPMLSILMDKGTGAVTSVPSDAPDDYMAL---LDLKSKPAFRAKYGVKDEWVMPFEI 404
>TC13072 similar to UP|Q9FR53 (Q9FR53) PTOR, partial (8%)
Length = 616
Score = 28.1 bits (61), Expect = 7.0
Identities = 23/95 (24%), Positives = 39/95 (40%)
Frame = +1
Query: 277 IEGFAKIVGPLTQLTRKDQPFAWTEACKTSFQTLKERLTTSPVLILPQPEEPYEVYCDAS 336
IEG K++ PL ++ E K + T+KER+ + + YE +
Sbjct: 322 IEGMLKVLEPLHEMLE--------EGAKKNNVTIKERVFIEAYR--QELLDAYECCMNYK 471
Query: 337 HQGLGCVLMQHWKVVAYASRQLKTHEKNYPTHDLE 371
G L Q W + + R++ ++ T DLE
Sbjct: 472 RTGKDAELTQAWDIYYHVYRKIDKQLQSLTTLDLE 576
>TC9644 homologue to UP|Q9M394 (Q9M394) Fructokinase-like protein, partial
(17%)
Length = 552
Score = 28.1 bits (61), Expect = 7.0
Identities = 15/46 (32%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Frame = -1
Query: 343 VLMQHWKVVAYASRQLKTHEKNYPTHD-LELTAIVFSLKIWRHYLY 387
+L+Q W V+ Y + K HE N D + L A + ++ R Y+Y
Sbjct: 465 LLIQKWYVLGYT*VEAKVHENNLSNLDFVLLPAPILD*RLSREYIY 328
>TC18086 similar to UP|O81023 (O81023) At2g26840 protein, partial (47%)
Length = 954
Score = 27.7 bits (60), Expect = 9.1
Identities = 14/42 (33%), Positives = 21/42 (49%), Gaps = 5/42 (11%)
Frame = +3
Query: 287 LTQLTRKDQPFAWTEACKTSFQTLKER-----LTTSPVLILP 323
L + + + AW E C T+F L+ LT +P L+LP
Sbjct: 750 LLHMAKTKRRIAWKEPCHTNFDKLETSIFYIFLTFTPALLLP 875
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.141 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,876,625
Number of Sequences: 28460
Number of extensions: 232513
Number of successful extensions: 1338
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1330
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1337
length of query: 968
length of database: 4,897,600
effective HSP length: 99
effective length of query: 869
effective length of database: 2,080,060
effective search space: 1807572140
effective search space used: 1807572140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0306.4