BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0303.2
(291 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP041113 182 6e-47
TC13572 135 6e-33
AV418280 114 1e-26
AV412895 111 2e-25
AV427022 106 5e-24
TC20217 99 8e-22
TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular so... 95 2e-20
AV414139 89 1e-18
BP056597 82 1e-16
BP057427 72 8e-14
AV428268 70 6e-13
AU089037 62 9e-11
TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, part... 62 9e-11
BP033775 52 1e-09
TC15601 57 3e-09
TC14967 53 3e-09
BP056818 55 1e-08
BP040769 54 3e-08
AU089120 52 1e-07
BP034765 51 3e-07
>BP041113
Length = 503
Score = 182 bits (462), Expect = 6e-47
Identities = 87/170 (51%), Positives = 110/170 (64%)
Frame = +1
Query: 12 FWHDDWLGTGCLRGAFYRLYNLSRQKWNCVNTLGSWEHGRWHWKFLWRRPLVGRELEWEK 71
FW +DW+G G LR +YRL+NLS++K C+ G W HG W WK WRR LVGREL W +
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLSKEKRCCILECGGWNHGVWTWKLSWRRALVGRELGWLE 180
Query: 72 GLLDALGTVQLTKGVPDAWVWMPSNVGIYSVNSAYHFLQEPTLPDTDEALVSIWHSFAPS 131
++ L V L++GV D W+W+P G Y+VNSAY FLQ PTL DTD +IW + APS
Sbjct: 181 LMMKDLSGVCLSEGVEDKWLWLPG--GTYTVNSAYSFLQAPTLADTDPIFATIWRTVAPS 354
Query: 132 NVKGFAWRVLLDRFASRENLLKRQVITSLADAQCALCNDYVETGFHLLFS 181
VK FAWR LL R + +NL+KRQV+ A C C VE+ HLLF+
Sbjct: 355 -VKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESVTHLLFA 501
>TC13572
Length = 534
Score = 135 bits (341), Expect = 6e-33
Identities = 60/112 (53%), Positives = 75/112 (66%)
Frame = -3
Query: 2 KILKSGDVTDFWHDDWLGTGCLRGAFYRLYNLSRQKWNCVNTLGSWEHGRWHWKFLWRRP 61
K+L+SG+ T FW ++W G G LR +YRLYNLS+ KW ++ G W G W+W F WRRP
Sbjct: 358 KVLRSGNHTRFWLENWTGVGILREKYYRLYNLSKLKWASIDECGGWSQGVWNWDFRWRRP 179
Query: 62 LVGRELEWEKGLLDALGTVQLTKGVPDAWVWMPSNVGIYSVNSAYHFLQEPT 113
L GREL+W + L + V L GVPD WVW PS G YSVNSA+ FLQ P+
Sbjct: 178 LAGRELDWLQALSLDIVRVPLLAGVPDKWVWKPSEDGSYSVNSAFIFLQVPS 23
>AV418280
Length = 398
Score = 114 bits (286), Expect = 1e-26
Identities = 52/129 (40%), Positives = 72/129 (55%), Gaps = 1/129 (0%)
Frame = -2
Query: 162 DAQCALCNDYVETGFHLLFSCTFSLSVWQGCLHWLGCSFIQPSSARDHLSHFRFGINKLQ 221
D C C E HL F+C FS+ VWQ WLG S P+S + + F
Sbjct: 397 DLTCPFCTTVEECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLANFAQFSITARNKN 218
Query: 222 QRLA-LSIWLAVIWSVWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSD 280
QRL L+IW+A +WS+W+ RN ++FR A + +L+L++ RSW W KA GF+YS +
Sbjct: 217 QRLGELAIWIATVWSLWIQRNSIIFRNNALDHSYLLDLIQSRSWHWLKAKFCGFTYSLYE 38
Query: 281 WFSNPLLCI 289
W S PL C+
Sbjct: 37 WKSCPLECL 11
>AV412895
Length = 405
Score = 111 bits (277), Expect = 2e-25
Identities = 61/134 (45%), Positives = 78/134 (57%), Gaps = 1/134 (0%)
Frame = +1
Query: 98 GIYSVNSAYHFLQEPTLPDTDEALVSIWHSFAPSNVKGFAWRVLLDRFASRENLLKRQVI 157
G +SVNS++ FL + L + D IW AP N+K F WRVLL R +R+NLLKRQVI
Sbjct: 4 GGFSVNSSFVFL*DQYLEEPDPVFN*IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVI 183
Query: 158 TSLADAQCALCNDYVETGFHLLFSCTFSLSVWQGCLHWLGCSFIQPSSARDHLSHF-RFG 216
+ DA C LC E+G HLLFSC S+ +W C WLG S Q S + HL F G
Sbjct: 184 HNALDAICPLCGLAEESGSHLLFSCAESMLIWYECFAWLGVSTAQVSDPKVHLLQFSSIG 363
Query: 217 INKLQQRLALSIWL 230
+K Q+ +IW+
Sbjct: 364 RSKAQKLGETTIWM 405
>AV427022
Length = 429
Score = 106 bits (264), Expect = 5e-24
Identities = 54/137 (39%), Positives = 81/137 (58%), Gaps = 1/137 (0%)
Frame = +1
Query: 155 QVITSLADAQCALCNDYVETGFHLLFSCTFSLSVWQGCLHWLGCSFIQPSSARDHLSHFR 214
QV+ + +A C LC E+G HLLFSC+ S+ + C WLG S Q S + HL F
Sbjct: 10 QVLHNALEAICPLCCLAEESGSHLLFSCSNSMLI*YECHAWLGVSTAQVSDPKVHLLQFS 189
Query: 215 -FGINKLQQRLALSIWLAVIWSVWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKG 273
G +K Q+ +IW++V+WS+W RN VVF GG + +LE ++ +W+W ++ +
Sbjct: 190 SIGWSKAQKLGESAIWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDD 369
Query: 274 FSYSSSDWFSNPLLCIL 290
F YS +W SNP +C+L
Sbjct: 370 F*YSWYEWKSNPRICLL 420
>TC20217
Length = 561
Score = 99.0 bits (245), Expect = 8e-22
Identities = 68/196 (34%), Positives = 94/196 (47%), Gaps = 10/196 (5%)
Frame = +1
Query: 73 LLDALGTVQLTKGVPDAWVWMPSNVGIYSVNSAYHFLQEPT----LPDTDEALVSIWHSF 128
+LD +V L PD W W+PS GI + F +P + S+W
Sbjct: 4 MLDEAKSVPLE---PDKWCWLPSTDGILQLTLFILFFSHRCCYLQIPFSSSEYGSLWQL- 171
Query: 129 APSNVKGFAWRVLLDRFASRENLLKRQVITSLADAQCALCNDYVETGFH------LLFSC 182
+ F RV+LD +NL K V + N+ F LLFSC
Sbjct: 172 --QM*RPFL*RVMLDWIPWLQNLWKHCV---------S*RNNL*GVAFGCGIL*ALLFSC 318
Query: 183 TFSLSVWQGCLHWLGCSFIQPSSARDHLSHFRFGINKLQQRLALSIWLAVIWSVWLARNE 242
SL +W+ C W+G P + R HL F+FG NK QR A +IWLAVIW++WL RNE
Sbjct: 319 PVSLDIWRHCYRWMGVCTTLPRNPRQHLL*FQFGGNKKHQRGADAIWLAVIWTLWLIRNE 498
Query: 243 VVFRGGAANIYSVLEL 258
++FR G +I +V+E+
Sbjct: 499 IIFRNGMLDIPAVMEV 546
>TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular
solute-binding family 3 protein, partial (3%)
Length = 503
Score = 94.7 bits (234), Expect = 2e-20
Identities = 44/115 (38%), Positives = 64/115 (55%)
Frame = +1
Query: 176 FHLLFSCTFSLSVWQGCLHWLGCSFIQPSSARDHLSHFRFGINKLQQRLALSIWLAVIWS 235
F F + L +W +WLG F+ P + DHL F Q+ LS+WLAV+W
Sbjct: 55 FTSFFLLSLGLEIWCRVFNWLGVCFVCPGTPVDHLLSFAGLFLLAQKDGILSVWLAVVWH 234
Query: 236 VWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCIL 290
VW+ RNE VFR G + LVK + W+W KA F++++ +WF+ P+LC+L
Sbjct: 235 VWVGRNEAVFREGVFLPECIFYLVKLKVWEWLKARSCHFTHATYEWFNEPILCLL 399
>AV414139
Length = 309
Score = 88.6 bits (218), Expect = 1e-18
Identities = 41/102 (40%), Positives = 51/102 (49%)
Frame = -2
Query: 41 VNTLGSWEHGRWHWKFLWRRPLVGRELEWEKGLLDALGTVQLTKGVPDAWVWMPSNVGIY 100
+ +G W +G W W+F WRR L RE E L+ AL V L +G D W W IY
Sbjct: 308 IGGMGQWHNGVWEWEFRWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIY 129
Query: 101 SVNSAYHFLQEPTLPDTDEALVSIWHSFAPSNVKGFAWRVLL 142
+V SAY L +P E +W APSN F WR+LL
Sbjct: 128 TVRSAY*LLLDPIADSDSEVYEKVWVGIAPSNANAFVWRLLL 3
>BP056597
Length = 414
Score = 82.0 bits (201), Expect = 1e-16
Identities = 43/131 (32%), Positives = 63/131 (47%), Gaps = 1/131 (0%)
Frame = +3
Query: 27 FYRLYNLSRQKWNC-VNTLGSWEHGRWHWKFLWRRPLVGRELEWEKGLLDALGTVQLTKG 85
F L+ +S Q+ NC + +G W +W W F W+ P+ G ++ + + + L G
Sbjct: 9 FDSLFLISNQQ-NCKIVEMGHWLENKWVWDFSWKNPISGEDVLKLGVMKQIVSSFSLING 185
Query: 86 VPDAWVWMPSNVGIYSVNSAYHFLQEPTLPDTDEALVSIWHSFAPSNVKGFAWRVLLDRF 145
D WVW+ G YSV SAY L +W + APSN WRV LDR
Sbjct: 186 KKDTWVWILEGDGQYSVKSAYDLLSGLDTTIGISVFSKLWKACAPSNAVALGWRVFLDRI 365
Query: 146 ASRENLLKRQV 156
+++NL +R V
Sbjct: 366 QTKDNLSRRHV 398
>BP057427
Length = 480
Score = 72.4 bits (176), Expect = 8e-14
Identities = 33/88 (37%), Positives = 53/88 (59%), Gaps = 1/88 (1%)
Frame = -1
Query: 203 PSSARDHLSHFRFGINKLQQRL-ALSIWLAVIWSVWLARNEVVFRGGAANIYSVLELVKR 261
P A + F GI L++R A SIW+A + S+W +RN ++F+G + +++ ++
Sbjct: 477 PIEASVNFLQFMQGIGNLKRRRGAWSIWVAALASIWYSRNSLIFKGEELDFQRLVDQIQT 298
Query: 262 RSWQWNKANMKGFSYSSSDWFSNPLLCI 289
+SW W KA + GFSYS +W S PL C+
Sbjct: 297 KSWSWLKAKVTGFSYSLIEWKSQPLSCL 214
>AV428268
Length = 429
Score = 69.7 bits (169), Expect = 6e-13
Identities = 42/143 (29%), Positives = 63/143 (43%), Gaps = 1/143 (0%)
Frame = +3
Query: 100 YSVNSAYHFLQEPTLPDTDEALVSIWHSFAPSNVKGFAWRVLLDRFASRENLLKRQVITS 159
YSV SAY FL D +W PSN +W+VL++R ++ NL +R V+ S
Sbjct: 6 YSVKSAYDFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVIS 185
Query: 160 LADAQCALCNDYVETGFHLLFSCTFSLSVWQGCLHWLGCSFIQPSSARDHLSHFRFGINK 219
C LC+ E+ HLLFSC +W G + P+ + H
Sbjct: 186 NV---CPLCSLDEESTDHLLFSCPIV*RIWSKISE*FGVFSVFPNDSHGHFLQHLGSCGS 356
Query: 220 LQ-QRLALSIWLAVIWSVWLARN 241
L ++ +W+A + +W RN
Sbjct: 357 LNFRKRGWFVWIAAVVCIWQGRN 425
>AU089037
Length = 245
Score = 62.4 bits (150), Expect = 9e-11
Identities = 28/59 (47%), Positives = 37/59 (62%)
Frame = -1
Query: 124 IWHSFAPSNVKGFAWRVLLDRFASRENLLKRQVITSLADAQCALCNDYVETGFHLLFSC 182
+W APSN FAWR++LDR +R NL +RQVI +A C +C+ E+ HL FSC
Sbjct: 191 LWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLFFSC 15
>TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, partial (5%)
Length = 579
Score = 62.4 bits (150), Expect = 9e-11
Identities = 25/60 (41%), Positives = 40/60 (66%)
Frame = +3
Query: 230 LAVIWSVWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCI 289
+A +WS+ L RN ++F GG + ++L+ + RSW W K+ M GF+ S +W +NPL+CI
Sbjct: 15 MATVWSLXLVRNSILFSGGVLDKANILDSIPWRSWFWLKSKMDGFTSSLFEWKTNPLICI 194
>BP033775
Length = 530
Score = 52.4 bits (124), Expect(2) = 1e-09
Identities = 21/50 (42%), Positives = 33/50 (66%)
Frame = -2
Query: 240 RNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCI 289
RN+V+F G N +V++L+K ++W+W + K FSYSS W NP +C+
Sbjct: 394 RNDVLFNGKTINSEAVVDLIKFKAWKWLQCKTKDFSYSSYAWNWNPCICL 245
Score = 25.8 bits (55), Expect(2) = 1e-09
Identities = 11/43 (25%), Positives = 18/43 (41%)
Frame = -1
Query: 195 WLGCSFIQPSSARDHLSHFRFGINKLQQRLALSIWLAVIWSVW 237
WLG +Q R H + + ++W A IW++W
Sbjct: 530 WLGVITVQEEDPRRHFDQHGSTLVQSLGSGWWTVWCAGIWTIW 402
>TC15601
Length = 613
Score = 57.4 bits (137), Expect = 3e-09
Identities = 21/62 (33%), Positives = 38/62 (60%)
Frame = +2
Query: 228 IWLAVIWSVWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSDWFSNPLL 287
+ +A+ W++W RN ++F+ G + V+E+ + R+W W +A K F +S+ +W NP
Sbjct: 83 VCIAIAWTLWNLRNMIIFKEGHLDGERVVEIAQLRAWNWLRAKKKDFKFSTYEWVVNPRY 262
Query: 288 CI 289
CI
Sbjct: 263 CI 268
>TC14967
Length = 655
Score = 52.8 bits (125), Expect(2) = 3e-09
Identities = 24/62 (38%), Positives = 33/62 (52%)
Frame = +2
Query: 187 SVWQGCLHWLGCSFIQPSSARDHLSHFRFGINKLQQRLALSIWLAVIWSVWLARNEVVFR 246
S+W +WLG F+ P S DHL F + + LS+WL V+W + + RNE V R
Sbjct: 101 SIWCRVFNWLGVCFVCPGSPVDHLMSFAGLFLSVNKDGILSVWLVVVWHILVGRNEAVLR 280
Query: 247 GG 248
G
Sbjct: 281 EG 286
Score = 24.3 bits (51), Expect(2) = 3e-09
Identities = 10/36 (27%), Positives = 21/36 (57%)
Frame = +1
Query: 255 VLELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCIL 290
+ +LVK + W+W +A F+ ++ + +LC+L
Sbjct: 304 IFDLVKLKVWEWLRARSCHFTRATYELIEQ-ILCLL 408
>BP056818
Length = 589
Score = 55.1 bits (131), Expect = 1e-08
Identities = 21/56 (37%), Positives = 33/56 (58%)
Frame = +2
Query: 234 WSVWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCI 289
W++W RN V+F G S+ E + R+W W +KGF+ S+ +W + P+LCI
Sbjct: 242 WAIWQHRNYVIFMEGRNQDVSIFEGAQLRAWHWFTGRVKGFNCSTYEWLAQPILCI 409
>BP040769
Length = 549
Score = 53.9 bits (128), Expect = 3e-08
Identities = 27/64 (42%), Positives = 36/64 (56%)
Frame = -3
Query: 183 TFSLSVWQGCLHWLGCSFIQPSSARDHLSHFRFGINKLQQRLALSIWLAVIWSVWLARNE 242
+FSL L WLG + P SA D L F + ++ + +SIW+AV+W VWL RN
Sbjct: 238 SFSLKKGLRILQWLGKPMVVPVSAMDLLLQFLWDTSQKIREGLMSIWVAVVWGVWLGRNS 59
Query: 243 VVFR 246
VFR
Sbjct: 58 KVFR 47
Score = 38.9 bits (89), Expect = 0.001
Identities = 27/94 (28%), Positives = 42/94 (43%), Gaps = 1/94 (1%)
Frame = -3
Query: 33 LSRQKWNCVNTLGSWEHGRWHWKFLWRRPLVGRELEWEKGLLDALGTVQLTKGVPD-AWV 91
+S Q+ V +G W + W W W PL E + LL + + L KG+ W+
Sbjct: 376 ISLQQDLTVKEVGLWRNESWFWDLRWSVPLNNVENQQLTSLLALVSSFSLKKGLRILQWL 197
Query: 92 WMPSNVGIYSVNSAYHFLQEPTLPDTDEALVSIW 125
P V + +++ FL + T E L+SIW
Sbjct: 196 GKPMVVPVSAMDLLLQFLWD-TSQKIREGLMSIW 98
>AU089120
Length = 586
Score = 52.0 bits (123), Expect = 1e-07
Identities = 36/125 (28%), Positives = 59/125 (46%), Gaps = 10/125 (8%)
Frame = +2
Query: 149 ENLLKRQVITSLADAQCALCNDYVETGFHLLFSCTFSLSVWQGCLHWLGCSFIQPSSARD 208
E L +R++I +L+ + C E+ HL F C S ++W WL + + P +
Sbjct: 2 EFLKRRRIIQTLS----SFCLHSEESIPHLFFRCFQSWNLWSSVHRWLVFTVVTPEEGKM 169
Query: 209 HLSHF--RFGINKLQQRLALSIWLAVIWSVWLARNEVVF--------RGGAANIYSVLEL 258
H F + ++ L IWL+ IW +W RN+VVF RG + S++ +
Sbjct: 170 HFIMF*DYYSGRDIKNGLGF-IWLSTIWFIWHLRNKVVFNGRCFRLVRGFRFDSISIVAM 346
Query: 259 VKRRS 263
KR+S
Sbjct: 347 AKRKS 361
Score = 41.2 bits (95), Expect = 2e-04
Identities = 15/34 (44%), Positives = 23/34 (67%)
Frame = +3
Query: 256 LELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCI 289
L+ ++ RSW W K +KG +S +W SNPL+C+
Sbjct: 315 LDSIQYRSWLWLKGKVKGLCFSLYEWSSNPLVCL 416
>BP034765
Length = 506
Score = 50.8 bits (120), Expect = 3e-07
Identities = 21/59 (35%), Positives = 33/59 (55%)
Frame = -2
Query: 231 AVIWSVWLARNEVVFRGGAANIYSVLELVKRRSWQWNKANMKGFSYSSSDWFSNPLLCI 289
A+ W++W RN +VF GG S+ + + + QW A K F +S +W S P+LC+
Sbjct: 493 AMAWALWEHRNWIVFNGGLLQEDSIFDKAQLKVCQWLSAKGKDFHFSMYEWLSQPILCL 317
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.138 0.483
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,659,723
Number of Sequences: 28460
Number of extensions: 140596
Number of successful extensions: 1308
Number of sequences better than 10.0: 103
Number of HSP's better than 10.0 without gapping: 1264
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1292
length of query: 291
length of database: 4,897,600
effective HSP length: 89
effective length of query: 202
effective length of database: 2,364,660
effective search space: 477661320
effective search space used: 477661320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0303.2