BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0299.12
(418 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18914 300 3e-82
BI418768 290 2e-79
TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] ... 39 0.002
TC14527 similar to PIR|F86340|F86340 protein F2D10.34 [imported]... 37 0.006
AW164008 37 0.008
TC11796 similar to GB|AAP12870.1|30017273|BT006221 At4g35840 {Ar... 37 0.008
BP055954 36 0.013
TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-fi... 35 0.030
TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Ara... 34 0.039
AU252073 34 0.051
TC17278 similar to UP|Q8GT74 (Q8GT74) NEP1-interacting protein 2... 33 0.067
BP036228 33 0.067
AV419203 33 0.067
TC15481 similar to UP|Q9LUR1 (Q9LUR1) RING zinc finger protein-l... 33 0.067
TC12446 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 33 0.087
TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich... 33 0.11
AV408970 33 0.11
TC11970 similar to UP|Q9FQZ7 (Q9FQZ7) Avr9/Cf-9 rapidly elicited... 32 0.19
AV767390 32 0.19
BE122610 32 0.25
>TC18914
Length = 524
Score = 300 bits (768), Expect = 3e-82
Identities = 141/173 (81%), Positives = 154/173 (88%)
Frame = +1
Query: 145 AEHTAVSSGSHHCPYIAYVGPIHPSSSNSGGTVSEASNFNHWNGSSVPNDMPASFTFPAV 204
AEHTAVSS SH CPYIAY+GPIHPS+SNSGGTVSE SNF HWNG P+DMP S+T+PAV
Sbjct: 4 AEHTAVSSASHPCPYIAYIGPIHPSTSNSGGTVSEDSNFPHWNGPPRPSDMPTSYTYPAV 183
Query: 205 DLHYHGWEHHSPPFSTASSRLVAADQPSVSPGNQRPVRGGSDVPRSGSFMHPFLVGHSSA 264
DLHYH WEHHS FS+A+SRL ADQPSVS G QRP R GS+VPRSGSF+HPFLVGHSS
Sbjct: 184 DLHYHSWEHHSSHFSSATSRLGVADQPSVSAGTQRPARVGSEVPRSGSFVHPFLVGHSSG 363
Query: 265 ARAGSSVASSLIPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTPIASGTR 317
ARAG+SVASS+IPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTP+AS +R
Sbjct: 364 ARAGNSVASSMIPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTPVASSSR 522
>BI418768
Length = 546
Score = 290 bits (743), Expect = 2e-79
Identities = 130/134 (97%), Positives = 131/134 (97%)
Frame = +3
Query: 1 MGLGNNEDEGNQEVDGGDGGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIG 60
MGLGNNEDE NQ VDGGDGGG KGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIG
Sbjct: 105 MGLGNNEDEDNQVVDGGDGGGAKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIG 284
Query: 61 SAFNIKGAMQCPNCRKIEKGQWLYANGGRSYPEFNMDDWTYDEDLYDLSYSELQSFGVHW 120
SAFNIKGAMQCPNCRKIEKGQWLYANGGRSYPEFNMDDWTYDEDLYDLSYSELQSFGVHW
Sbjct: 285 SAFNIKGAMQCPNCRKIEKGQWLYANGGRSYPEFNMDDWTYDEDLYDLSYSELQSFGVHW 464
Query: 121 CPFGNLARLPSPFD 134
CPFGNLARLPSPF+
Sbjct: 465 CPFGNLARLPSPFE 506
>TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(58%)
Length = 591
Score = 38.5 bits (88), Expect = 0.002
Identities = 20/48 (41%), Positives = 26/48 (53%)
Frame = +1
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
C+ICL A GD CGH FH+ CI + +K CP+CR+I
Sbjct: 328 CAICLTEFAA-GDEIRVLPQCGHGFHVSCIDAW--LKSHSSCPSCRQI 462
>TC14527 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(42%)
Length = 1095
Score = 37.0 bits (84), Expect = 0.006
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = +2
Query: 18 DGGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
D + + C+ICL D GD CGH FH+ CI + + CP+CR++
Sbjct: 473 DSSPREWLATTECAICLAEF-DAGDEIRVLPQCGHGFHVGCIDTWLGSHSS--CPSCRQV 643
>AW164008
Length = 351
Score = 36.6 bits (83), Expect = 0.008
Identities = 18/49 (36%), Positives = 30/49 (60%)
Frame = +3
Query: 27 SVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
+V+C+IC +++ +S +L CGHQ+H DCI + + + CP CR
Sbjct: 189 TVACAICKDLMGVG--QSAKRLPCGHQYHGDCIVPWLSSRNS--CPVCR 323
>TC11796 similar to GB|AAP12870.1|30017273|BT006221 At4g35840 {Arabidopsis
thaliana;}, partial (86%)
Length = 1114
Score = 36.6 bits (83), Expect = 0.008
Identities = 20/49 (40%), Positives = 26/49 (52%)
Frame = +1
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRK 76
VSCS+CL+ G+ + HC H FHL CI G+ CP CR+
Sbjct: 604 VSCSVCLQDF-QLGETVRSLPHCHHMFHLPCIDKWLFRHGS--CPLCRR 741
>BP055954
Length = 518
Score = 35.8 bits (81), Expect = 0.013
Identities = 19/53 (35%), Positives = 26/53 (48%)
Frame = +1
Query: 23 KGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
+G G +CSICL + + C H FHL C+ S + G+ CP CR
Sbjct: 280 RGHGETTCSICLCEFKE-AEMLRMMPECRHHFHLCCLDSWLKLNGS--CPVCR 429
>TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-finger,
complete
Length = 2092
Score = 34.7 bits (78), Expect = 0.030
Identities = 18/47 (38%), Positives = 25/47 (52%)
Frame = +2
Query: 29 SCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
SC ICLE + D K CGH +H++CI ++K CP C+
Sbjct: 1781 SCVICLEEYKNMDDVGTLKT-CGHDYHVNCIKKWLSMK--KLCPICK 1912
>TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Arabidopsis
thaliana;}, partial (79%)
Length = 1297
Score = 34.3 bits (77), Expect = 0.039
Identities = 19/48 (39%), Positives = 25/48 (51%)
Frame = +3
Query: 28 VSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
++CS+CLE V N L C HQFH +CI +G CP C+
Sbjct: 876 LTCSVCLEQV--NVGDILRSLPCLHQFHANCIDPWLRQQGT--CPVCK 1007
>AU252073
Length = 258
Score = 33.9 bits (76), Expect = 0.051
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +1
Query: 23 KGFGSVSCSICLEVVADNGDRSWAKL--HCGHQFHLDCIGSAFNIKGAMQCPNCR 75
+ F S+ + C+ +AD +R ++ CGH FHL CI ++ CP CR
Sbjct: 4 EAFSSIEDTQCVICLADYKEREVLRIMPKCGHTFHLSCID--IWLRKQSTCPVCR 162
>TC17278 similar to UP|Q8GT74 (Q8GT74) NEP1-interacting protein 2, partial
(62%)
Length = 610
Score = 33.5 bits (75), Expect = 0.067
Identities = 20/62 (32%), Positives = 30/62 (48%)
Frame = +3
Query: 15 DGGDGGGGKGFGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNC 74
+ GD G + SCS+CL+ G+ + +C H FHL CI + + CP C
Sbjct: 285 NNGDASGQRD----SCSVCLQDF-QLGETVRSLPYCHHMFHLPCIDEW--LSKHVSCPLC 443
Query: 75 RK 76
R+
Sbjct: 444 RR 449
>BP036228
Length = 626
Score = 33.5 bits (75), Expect = 0.067
Identities = 24/75 (32%), Positives = 32/75 (42%), Gaps = 10/75 (13%)
Frame = +2
Query: 12 QEVDGGDGGGGKG----------FGSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGS 61
QE+ GG G K GS++CS C+++ DR CGH F L C
Sbjct: 338 QELVGGPSSGEKPPAAGGILDIFDGSINCSFCMQL----PDRP-VTTPCGHNFCLKCFEK 502
Query: 62 AFNIKGAMQCPNCRK 76
+G C NCR+
Sbjct: 503 WIG-QGKRTCANCRR 544
>AV419203
Length = 393
Score = 33.5 bits (75), Expect = 0.067
Identities = 15/46 (32%), Positives = 23/46 (49%)
Frame = +1
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
C +CLE + + L C H FH++CI + ++CP CR
Sbjct: 256 CPVCLEEFHEGNEVRG--LPCAHNFHVECIDEWLRLN--VKCPRCR 381
>TC15481 similar to UP|Q9LUR1 (Q9LUR1) RING zinc finger protein-like,
partial (32%)
Length = 706
Score = 33.5 bits (75), Expect = 0.067
Identities = 17/52 (32%), Positives = 24/52 (45%)
Frame = +1
Query: 26 GSVSCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCRKI 77
G C++CL ++G+ C H FH DCI F+ CP CR +
Sbjct: 412 GPPECAVCLSEF-ESGETGRVLPKCKHAFHTDCIDMWFHSHST--CPLCRTL 558
>TC12446 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (38%)
Length = 550
Score = 33.1 bits (74), Expect = 0.087
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = +2
Query: 29 SCSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNC 74
+CSICLE + D+ + C H+FHL CI + + QCP C
Sbjct: 200 ACSICLEDLCDSEPSTVTG--CKHEFHLQCILEW--CQRSSQCPMC 325
>TC15560 similar to SP|Q03173|NDPP_MOUSE NPC derived proline rich protein 1
(NDPP-1). {Mus musculus;}, partial (8%)
Length = 1134
Score = 32.7 bits (73), Expect = 0.11
Identities = 29/109 (26%), Positives = 41/109 (37%), Gaps = 1/109 (0%)
Frame = +3
Query: 213 HHSPPFSTASSRLVAADQPSVS-PGNQRPVRGGSDVPRSGSFMHPFLVGHSSAARAGSSV 271
HH P +TAS+ ++ PS S P P +P + P L H + S++
Sbjct: 102 HHYPLTTTASASSSSSSSPSSS*PSPSLPPPSPPPLPPPQA---PPLPSHLPSPEPSSTM 272
Query: 272 ASSLIPPYPGSNARARDRVQALQAYYQPQQPPNSTTMRTPIASGTRRPS 320
S PP P P QPP+S R P +S P+
Sbjct: 273 PPSTPPPQPTKPCPLPKST--------PSQPPSSAPHRVPTSSSLASPT 395
>AV408970
Length = 195
Score = 32.7 bits (73), Expect = 0.11
Identities = 13/21 (61%), Positives = 15/21 (70%)
Frame = -1
Query: 4 GNNEDEGNQEVDGGDGGGGKG 24
G D G +EV GGDGGGG+G
Sbjct: 159 GEGADGGEEEVAGGDGGGGEG 97
>TC11970 similar to UP|Q9FQZ7 (Q9FQZ7) Avr9/Cf-9 rapidly elicited protein
132, partial (24%)
Length = 789
Score = 32.0 bits (71), Expect = 0.19
Identities = 16/44 (36%), Positives = 23/44 (51%)
Frame = +1
Query: 32 ICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
+CL + D G+++ C H FH+DCI F + CP CR
Sbjct: 4 VCLSEL-DEGEKARLLPKCNHGFHVDCIDMWF--QSHSTCPLCR 126
>AV767390
Length = 447
Score = 32.0 bits (71), Expect = 0.19
Identities = 16/46 (34%), Positives = 21/46 (44%)
Frame = -3
Query: 30 CSICLEVVADNGDRSWAKLHCGHQFHLDCIGSAFNIKGAMQCPNCR 75
C ICL D + +L CGH FH C+ +I CP C+
Sbjct: 430 CCICLSSYDDGVELR--ELPCGHHFHCGCVDKWLHINAT--CPLCK 305
>BE122610
Length = 386
Score = 31.6 bits (70), Expect = 0.25
Identities = 14/32 (43%), Positives = 18/32 (55%), Gaps = 2/32 (6%)
Frame = +1
Query: 47 KLHCGHQFHLDCIGSAFN--IKGAMQCPNCRK 76
KL+C H FHL C+ S + + CP CRK
Sbjct: 25 KLNCNHLFHLACLRSWLDQGLTEIYTCPTCRK 120
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.133 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,354,473
Number of Sequences: 28460
Number of extensions: 166038
Number of successful extensions: 1486
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 1425
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1468
length of query: 418
length of database: 4,897,600
effective HSP length: 93
effective length of query: 325
effective length of database: 2,250,820
effective search space: 731516500
effective search space used: 731516500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0299.12