BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0279a.6
(1582 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 89 7e-18
BP063694 77 2e-14
AV780009 71 1e-12
AV766088 67 2e-11
TC8302 62 7e-10
BP033602 62 7e-10
AV766665 58 1e-08
TC9039 55 7e-08
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 47 2e-05
BP063902 44 3e-04
BP075943 39 0.005
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 38 0.011
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.093
BP058111 35 0.12
BI418821 34 0.16
AV421607 34 0.21
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 34 0.21
TC18909 34 0.21
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 33 0.27
AV773507 33 0.27
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 88.6 bits (218), Expect = 7e-18
Identities = 41/82 (50%), Positives = 59/82 (71%)
Frame = +2
Query: 1300 AKKILGMEIHREKGAKKLWLSQNSYVEGVLSRFDMSKANPVSTPLANHFKLSLEQCPKTA 1359
A KILGM+IHR++ +++WLSQ +Y++ VL RF+M NP+STPL ++KLS P +
Sbjct: 203 ANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSMIPSSE 382
Query: 1360 SEIEGMSKISHASAVGCLMYVM 1381
+E MS++ +ASAVG LMY M
Sbjct: 383 AERMEMSRVPYASAVGSLMYAM 448
>BP063694
Length = 511
Score = 77.4 bits (189), Expect = 2e-14
Identities = 42/118 (35%), Positives = 61/118 (51%), Gaps = 1/118 (0%)
Frame = -2
Query: 417 KLWHMRLGHLSERGMMELYKRNLLKGVRSCTIGLC-KYCVLGKQCRVRFKTGQHKTKGIL 475
+LWH RLGH++ + +L+K L +C C + K R+ F + +L
Sbjct: 363 ELWHSRLGHVNFDIIKQLHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAVL 184
Query: 476 DYVHSDVWGPTKEPSVGGFRYFVTFTDDFSRKVWVYFLKYKSEVFAKFKLWKAEVENQ 533
D +H D+ GP+ S+ GF YFV F DDFSR W Y LK KS+ +K +EN+
Sbjct: 183 DLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMENR 10
>AV780009
Length = 529
Score = 71.2 bits (173), Expect = 1e-12
Identities = 42/100 (42%), Positives = 59/100 (59%), Gaps = 1/100 (1%)
Frame = -1
Query: 1408 EAVKWILRYLKGTADRGIMFSREQGVVPLVV-GYVDSDYAGDLDDRRSTTGYVFTLAGGP 1466
+A +LRY+KG +G+ FS + PL + Y DSD+AG D RRS TGY L
Sbjct: 529 QAATRVLRYVKGAPAQGLFFSADS---PLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSL 359
Query: 1467 ICWKSSVQSIVAMSTTEAEYMAVAEAVKEALWLTGLVKKL 1506
I W++ Q+ V+ S++EAEY A+A V E WL+ L + L
Sbjct: 358 ISWRTKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFL 239
>AV766088
Length = 501
Score = 67.0 bits (162), Expect = 2e-11
Identities = 37/106 (34%), Positives = 53/106 (49%)
Frame = -3
Query: 415 ATKLWHMRLGHLSERGMMELYKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTKGI 474
A LWH RLGH S + L + +C+ CV GK R+ F + + T
Sbjct: 316 AQSLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMP 137
Query: 475 LDYVHSDVWGPTKEPSVGGFRYFVTFTDDFSRKVWVYFLKYKSEVF 520
D +HSD+W + S G R++V F DDF+ +W + L KS+VF
Sbjct: 136 FDILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>TC8302
Length = 494
Score = 62.0 bits (149), Expect = 7e-10
Identities = 42/145 (28%), Positives = 68/145 (45%), Gaps = 2/145 (1%)
Frame = +3
Query: 102 QRLYSLKMQEGGDLQAHVYAFNNILADLTRLGVTVDDEDKTIILLCSLPGSYDHLVTTLT 161
+RLY+L+M E + H+ N + A L+ T+ + ++ +LL SLP SYD LV +
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 162 YGK--DSITLDSISSTLLQHAQRRRSVEEGGGSSGEGLFVKGGQDRGRGKGKAVDSGKKK 219
+ + L +S LL+ +R + E+ SS K + +
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSS---------------KPMEALTMTRG 317
Query: 220 RSKSKDRKTAECYSCKQIGHWKRDC 244
RSKS+ + +CY C Q H K+DC
Sbjct: 318 RSKSRKKINLKCYHCGQR*HLKKDC 392
>BP033602
Length = 533
Score = 62.0 bits (149), Expect = 7e-10
Identities = 35/90 (38%), Positives = 50/90 (54%)
Frame = +3
Query: 414 DATKLWHMRLGHLSERGMMELYKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTKG 473
D LWH RLGH + + + L+ +L K V +C+ C+ CVL K R + + +
Sbjct: 264 DQIMLWHNRLGHPNFQYLRHLFP-DLFKNV-NCSSLECESCVLAKNQRAPYYSQPYHASR 437
Query: 474 ILDYVHSDVWGPTKEPSVGGFRYFVTFTDD 503
+HSDVWGP+K + G R+FVTF DD
Sbjct: 438 PFYLIHSDVWGPSKITTQFGKRWFVTFIDD 527
>AV766665
Length = 601
Score = 58.2 bits (139), Expect = 1e-08
Identities = 34/90 (37%), Positives = 49/90 (53%), Gaps = 4/90 (4%)
Frame = +3
Query: 1403 GKQHWEAVKW----ILRYLKGTADRGIMFSREQGVVPLVVGYVDSDYAGDLDDRRSTTGY 1458
GK W ++K I YLK + RG +F +E + G+ +DY G + DR ST GY
Sbjct: 333 GKNRWSSLKTFTTRIF*YLKANSRRGPLFQKEGK--SSMDGFTYADYLGSIVDRLSTMGY 506
Query: 1459 VFTLAGGPICWKSSVQSIVAMSTTEAEYMA 1488
L+G + W+S Q+I+A S+ EAE A
Sbjct: 507 YMFLSGNLVTWRSKQQNIIARSSGEAELRA 596
>TC9039
Length = 1218
Score = 55.5 bits (132), Expect = 7e-08
Identities = 37/149 (24%), Positives = 67/149 (44%), Gaps = 6/149 (4%)
Frame = +2
Query: 102 QRLYSLKMQEGGDLQAHVYAFNNILADLTRLGVTVDDEDKTIILLCSLPGSYDHLVTTLT 161
Q L S+K Q G+++ ++ +NI + L L + + D+ ++L SLP + +
Sbjct: 467 QNLISMKYQGKGNIREYIMGMSNIASKLKALKLELSDDLLIHLVLLSLPAQFSQFKISYN 646
Query: 162 YGKDSITLDSISSTLLQHAQRRRSVEEGGGSSGEGLFVKGGQDRGRGK------GKAVDS 215
K+ +L+ + S +Q +R + + FV +D+G+ K +A D+
Sbjct: 647 CPKEKWSLNELISFCVQEEERLKQERKESAH-----FVSTSKDKGKRKKTVEPKNEAADA 811
Query: 216 GKKKRSKSKDRKTAECYSCKQIGHWKRDC 244
K+ K D CY C GH K+ C
Sbjct: 812 PAPKKQKEDDT----CYFCNVSGHMKKKC 886
Score = 36.6 bits (83), Expect = 0.032
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = +3
Query: 311 YVYLGDDKPCIIKGM*QVKIALDDGGVRTLSQVRYVPEVTKNLISLGTLHENGYS 365
Y+Y+GD K ++ + ++ L G L VP +NLIS+ L ++GYS
Sbjct: 957 YIYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLDKSGYS 1121
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 47.4 bits (111), Expect = 2e-05
Identities = 20/44 (45%), Positives = 28/44 (63%)
Frame = +3
Query: 1461 TLAGGPICWKSSVQSIVAMSTTEAEYMAVAEAVKEALWLTGLVK 1504
T AGG + W S +Q VA+ST EAE++A EA E LW+ ++
Sbjct: 9 TFAGGAVAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQ 140
>BP063902
Length = 494
Score = 43.5 bits (101), Expect = 3e-04
Identities = 29/95 (30%), Positives = 45/95 (46%)
Frame = -3
Query: 372 RDILRVSKGAMTVMRAKRTAGNIYKLLGSTVMGDVASVETDDDATKLWHMRLGHLSERGM 431
R ILR K M ++R + G++Y + S+ +AS +WH RLGH + +
Sbjct: 294 RPILRHLKTGMPLLRCN-SLGDLYPVTRSSPFAGLAS--------SVWHHRLGHPASSAL 142
Query: 432 MELYKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKT 466
L L+ S + +C CVLGK R+ F +
Sbjct: 141 NHLRNNKLIFCEPSRSSSVCDSCVLGKHVRLPFSS 37
>BP075943
Length = 547
Score = 39.3 bits (90), Expect = 0.005
Identities = 38/145 (26%), Positives = 64/145 (43%), Gaps = 2/145 (1%)
Frame = -3
Query: 1300 AKKILGMEIHREKGAKKLWLSQNSYVEGVLSRFDMSKANPVSTPLANHFKLSLEQCPKTA 1359
AK LG+EI R + L+Q Y ++S P +H S T
Sbjct: 413 AKYFLGLEIARSTSG--IVLNQRKYALQLISDSGHFGFQP---RFYSHGTNSQTLGTNTG 249
Query: 1360 SEIEGMSKISHASAVGCLMYVMVCTRPDLAQAASQVCKFMSKPGKQHWEAVKWILRYLKG 1419
+ + + S+ VG L+Y+ TRPD+ A +Q+ +F+S P H + + L+YL G
Sbjct: 248 TPLTDIG--SYRRIVGRLLYLNT-TRPDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*G 78
Query: 1420 TADRGIMFSREQGV--VPLVVGYVD 1442
+ G+ + +P ++G D
Sbjct: 77 SPGSGLFYPASSSTL*LPSLIGGPD 3
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 38.1 bits (87), Expect = 0.011
Identities = 40/154 (25%), Positives = 66/154 (41%), Gaps = 1/154 (0%)
Frame = -1
Query: 1321 QNSYVEGVLSRFDMSKANPVSTPLANHFKLSLEQCPKTASEIEGMSKISHASAVGCLMYV 1380
Q YV+ +L +F M+ + +ST + +E + + + + S +G + Y+
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGK---EIEAGRRNGGK--RVDSTYYKSLIGSVRYL 575
Query: 1381 MVCTRPDLAQAASQVCKFMSKPGKQHWEAVKWILRYLKGTADRGIMFSREQGVVPLVVGY 1440
R D+ +FM +P H + + LRY+KGT GI LV+
Sbjct: 574 NT-VRSDIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFM--------LVITM 425
Query: 1441 VDSDYAGDLDDRRSTT-GYVFTLAGGPICWKSSV 1473
+S LD + T GY F L G I W S++
Sbjct: 424 *NS-----LDTQIVTRPGYAFHLGRGAISWSSTI 338
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 35.0 bits (79), Expect = 0.093
Identities = 24/70 (34%), Positives = 31/70 (44%)
Frame = +1
Query: 186 VEEGGGSSGEGLFVKGGQDRGRGKGKAVDSGKKKRSKSKDRKTAECYSCKQIGHWKRDCP 245
VE S G G GG+ GRG+G +D K CY C + GH+ R+C
Sbjct: 88 VELSHNSKGGG----GGRGGGRGRG------------GEDLK---CYECGEPGHFARECR 210
Query: 246 NRSGKSGNSS 255
+R G G S
Sbjct: 211 SRGGSRGLGS 240
>BP058111
Length = 570
Score = 34.7 bits (78), Expect = 0.12
Identities = 19/63 (30%), Positives = 29/63 (45%)
Frame = -1
Query: 418 LWHMRLGHLSERGMMELYKRNLLKGVRSCTIGLCKYCVLGKQCRVRFKTGQHKTKGILDY 477
LWH+RLGH S + + L K + T C C + KQ ++ F + I D
Sbjct: 186 LWHLRLGHTSSKKLAILQKNFPFITCKKIT-SPCDTCHMAKQKKLSFPNSVTLSSEIFDL 10
Query: 478 VHS 480
+H+
Sbjct: 9 IHT 1
>BI418821
Length = 614
Score = 34.3 bits (77), Expect = 0.16
Identities = 22/74 (29%), Positives = 27/74 (35%), Gaps = 6/74 (8%)
Frame = +2
Query: 189 GGGSSGEGLFVKGGQDRGRGKGKAVDSGKKKRSKSKDRKT------AECYSCKQIGHWKR 242
GG GE GG G G D+G R + A CY+C GH R
Sbjct: 332 GGWRGGERRNGGGGGGGGGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLAR 511
Query: 243 DCPNRSGKSGNSSS 256
DC + SG +
Sbjct: 512 DCNRSNNNSGGGGA 553
Score = 30.8 bits (68), Expect = 1.8
Identities = 17/59 (28%), Positives = 21/59 (34%)
Frame = +2
Query: 200 KGGQDRGRGKGKAVDSGKKKRSKSKDRKTAECYSCKQIGHWKRDCPNRSGKSGNSSSAA 258
+GG+ R G G G CY+C GH RDC + G AA
Sbjct: 341 RGGERRNGGGGGGGGGG--------------CYNCGDTGHLARDCHRSNNNGGGGGGAA 475
Score = 30.0 bits (66), Expect = 3.0
Identities = 25/81 (30%), Positives = 31/81 (37%), Gaps = 21/81 (25%)
Frame = +2
Query: 189 GGGSSGEGLFVKG-----------GQDRGRGKGKAV-----DSGKKKRSKSKDRKT---- 228
GGG G G + G + G G G A D+G R ++
Sbjct: 368 GGGGGGGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGG 547
Query: 229 -AECYSCKQIGHWKRDCPNRS 248
A CY+C GH RDC NRS
Sbjct: 548 GAGCYNCGDTGHLARDC-NRS 607
>AV421607
Length = 245
Score = 33.9 bits (76), Expect = 0.21
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 231 CYSCKQIGHWKRDCPNRSGKS 251
CY C + GHW RDCP+ + S
Sbjct: 54 CYKCGRPGHWSRDCPSSAPNS 116
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 33.9 bits (76), Expect = 0.21
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 450 LCKYCVLGKQCRVRFKTGQHKTKGILDYVHSDVW 483
+C CVLGK R+ F + + T D +HSD+W
Sbjct: 1026 VCDSCVLGKHVRLPFSSSETITLRPFDILHSDLW 1127
>TC18909
Length = 621
Score = 33.9 bits (76), Expect = 0.21
Identities = 24/87 (27%), Positives = 39/87 (44%), Gaps = 1/87 (1%)
Frame = +2
Query: 1384 TRPDLAQAASQVCKFMSKPGKQHWEAVKWILRYL-KGTADRGIMFSREQGVVPLVVGYVD 1442
T P ++ + ++VC F+S+ ++H A K IL K G + D
Sbjct: 326 TSPQISFSVNKVC*FLSESLEEHGTAGKCILNVT*KAL*TMGFFIQTN------FPAFSD 487
Query: 1443 SDYAGDLDDRRSTTGYVFTLAGGPICW 1469
+D+A ++DDRRST + G W
Sbjct: 488 ADWASNVDDRRSTFWEIVFTLGPKFRW 568
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 33.5 bits (75), Expect = 0.27
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 4/50 (8%)
Frame = +2
Query: 201 GGQDRGRGKGKAVDSGKKKRSK----SKDRKTAECYSCKQIGHWKRDCPN 246
G + R RG+ + D G + RS+ S+ EC+ C + GH+ R+CP+
Sbjct: 320 GDRYRERGRDRD-DRGDRDRSRGYGGSRGSNGGECFKCGKPGHFARECPS 466
>AV773507
Length = 496
Score = 33.5 bits (75), Expect = 0.27
Identities = 19/62 (30%), Positives = 28/62 (44%), Gaps = 2/62 (3%)
Frame = +2
Query: 201 GGQDRGRG-KGKAVDSGKKKRSKSKDRKTAE-CYSCKQIGHWKRDCPNRSGKSGNSSSAA 258
GG D +G +G SG + + DR + C+ C + GH RDCP G G
Sbjct: 254 GGDDADQGYRGGGYSSGGRGSYGAGDRVGQDDCFECGRPGHRARDCPLAGGGGGRGRGGG 433
Query: 259 NV 260
++
Sbjct: 434 SL 439
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.341 0.149 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,589,527
Number of Sequences: 28460
Number of extensions: 452495
Number of successful extensions: 3903
Number of sequences better than 10.0: 57
Number of HSP's better than 10.0 without gapping: 3704
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3841
length of query: 1582
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1480
effective length of database: 1,994,680
effective search space: 2952126400
effective search space used: 2952126400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0279a.6