BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0276.3
(333 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP049512 52 6e-09
AV406816 40 4e-04
TC7942 similar to UP|Q8LPE6 (Q8LPE6) Epoxide hydrolase (Fragmen... 39 0.001
TC16751 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase ... 39 0.001
TC7941 similar to UP|O49857 (O49857) Epoxide hydrolase , partia... 36 0.008
TC15968 36 0.010
TC16547 similar to UP|O49857 (O49857) Epoxide hydrolase , parti... 35 0.018
TC11862 similar to UP|Q39856 (Q39856) Epoxide hydrolase , parti... 34 0.030
TC17917 similar to GB|AAO63324.1|28950801|BT005260 At5g06770 {Ar... 31 0.25
BP067888 29 1.3
BP048208 27 3.7
AU252197 27 3.7
AU251527 27 4.8
TC19109 similar to UP|Q84LE0 (Q84LE0) Phytocyanin protein, PUP2,... 26 8.2
TC11665 similar to UP|AAR37673 (AAR37673) Cytochrome c-type biog... 26 8.2
>BP049512
Length = 519
Score = 52.4 bits (124), Expect(2) = 6e-09
Identities = 24/25 (96%), Positives = 25/25 (100%)
Frame = -1
Query: 250 FSSDSYLLDGVDKIRHINTTIVQGR 274
FSSDSYLLDG+DKIRHINTTIVQGR
Sbjct: 519 FSSDSYLLDGLDKIRHINTTIVQGR 445
Score = 23.9 bits (50), Expect(2) = 6e-09
Identities = 9/14 (64%), Positives = 10/14 (71%), Gaps = 1/14 (7%)
Frame = -2
Query: 275 YDVCCPM-MSAWDL 287
YDVCCP +WDL
Sbjct: 443 YDVCCPYDCQSWDL 402
>AV406816
Length = 417
Score = 40.4 bits (93), Expect = 4e-04
Identities = 32/100 (32%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +1
Query: 47 VVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKL 105
VVFLHG P S ++ +R I D RG G S P E+ T L+ D+ +
Sbjct: 112 VVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRGYGLSDPPPEPENATFSVLLSDLLAI 291
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L + + + G +GS A +S HP++V G+I G+
Sbjct: 292 LDALALSKVFLVGKDFGSRPAYLFSILHPERVLGVITFGV 411
>TC7942 similar to UP|Q8LPE6 (Q8LPE6) Epoxide hydrolase (Fragment) ,
partial (91%)
Length = 1288
Score = 39.3 bits (90), Expect = 0.001
Identities = 34/129 (26%), Positives = 54/129 (41%), Gaps = 5/129 (3%)
Frame = +3
Query: 44 GHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + + + T + L+ DI
Sbjct: 66 GPVVLFLHGFPELWYSWRHQILSLSSRGYRAVAPDLRGYGDTEAPSSVTSYTCFHLVGDI 245
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI----FLLRKKEIDWFYE 158
L + L + + + G WG+ L PD++ + G+ FL R +
Sbjct: 246 IALIDSLGVDKVYLVGHDWGAVLGWYVCLFRPDRIKAYVCLGVTFRPFLARNPNVKTL-- 419
Query: 159 GGAAAIFPD 167
G A+F D
Sbjct: 420 DGFRAMFGD 446
>TC16751 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase , partial
(55%)
Length = 638
Score = 38.9 bits (89), Expect = 0.001
Identities = 32/100 (32%), Positives = 45/100 (45%), Gaps = 1/100 (1%)
Frame = +2
Query: 47 VVFLHGGPGGGTSPSNRRFFDPEF-YRIILFDQRGAGKSTPHACLEHNTTWDLIDDIEKL 105
VVFLHG P + ++ YR I FD RG G S A E DL+ + L
Sbjct: 101 VVFLHGFPEIWYTWRHQMIAVANAGYRAIAFDFRGYGLSEHPAEPEKANLMDLVGETVGL 280
Query: 106 REHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI 145
+ L I + + G +G+ + HP+KV +I GI
Sbjct: 281 LDSLGINKAILVGKDFGAIPGYLVAALHPEKVASVITLGI 400
>TC7941 similar to UP|O49857 (O49857) Epoxide hydrolase , partial (48%)
Length = 610
Score = 36.2 bits (82), Expect = 0.008
Identities = 26/99 (26%), Positives = 43/99 (43%), Gaps = 1/99 (1%)
Frame = +2
Query: 44 GHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ YR + D RG G + + + T + L+ DI
Sbjct: 182 GPVVLFLHGFPELWYSWRHQILSLSSRGYRAVAPDLRGYGDTEAPSSVTSYTCFHLVGDI 361
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
L + L + + + G WG+ L PD++ +
Sbjct: 362 IALIDSLGVDKVYLVGHDWGAVLGWYVCLFRPDRIKAYV 478
>TC15968
Length = 689
Score = 35.8 bits (81), Expect = 0.010
Identities = 37/127 (29%), Positives = 56/127 (43%), Gaps = 10/127 (7%)
Frame = +2
Query: 28 VSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPSNRRFFDPEF---YRIILFDQRGAGKS 84
V + H + SG + +V HG GT S + F P +R+IL+D GAG +
Sbjct: 92 VEEAHNVKVLGSGTRA---IVLAHGF---GTDQSLWKHFVPYLTNDFRVILYDNMGAGTT 253
Query: 85 TP-------HACLEHNTTWDLIDDIEKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKV 137
P H+ LE +DL+ +E+LR + G S + + S S PD
Sbjct: 254 NPEYFDFERHSSLE-GYAYDLLSILEELR----VDSCIFVGHSVSAMIGAVASISRPDLF 418
Query: 138 TGIILRG 144
+ II+ G
Sbjct: 419 SKIIMVG 439
>TC16547 similar to UP|O49857 (O49857) Epoxide hydrolase , partial (50%)
Length = 550
Score = 35.0 bits (79), Expect = 0.018
Identities = 29/112 (25%), Positives = 46/112 (40%), Gaps = 2/112 (1%)
Frame = +1
Query: 44 GHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ Y + D RG G + + T + ++ DI
Sbjct: 112 GPVVLFLHGFPELWYSWRHQILTLSSRGYHAVAPDLRGYGDTDSPISITTYTCFHIVGDI 291
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI-FLLRKKEI 153
L +HL + + WGS + P++V + + FL R EI
Sbjct: 292 VALIDHLGAKQVFLVAHDWGSIIGWYLCMFRPERVKAYVCLSVPFLPRNPEI 447
>TC11862 similar to UP|Q39856 (Q39856) Epoxide hydrolase , partial (50%)
Length = 564
Score = 34.3 bits (77), Expect = 0.030
Identities = 24/99 (24%), Positives = 43/99 (43%), Gaps = 1/99 (1%)
Frame = +1
Query: 44 GHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P S ++ + YR + D RG G + + ++++ DI
Sbjct: 76 GPVVLFLHGFPELWYSWRHQILALSSQGYRAVAPDLRGYGDTEAPPSVTSYNCFNIVGDI 255
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGII 141
L + L + + + G WG+ + PDK+ +
Sbjct: 256 VALIDSLGVDQVFLVGHDWGAIIGWYVCLFRPDKIKAYV 372
>TC17917 similar to GB|AAO63324.1|28950801|BT005260 At5g06770 {Arabidopsis
thaliana;}, partial (35%)
Length = 587
Score = 31.2 bits (69), Expect = 0.25
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +1
Query: 16 PHIEPYSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGG 56
P +E ++TGL S T ++ SG P G FLH PGG
Sbjct: 160 PEMESFTTGLGSKSKPCTKFFSTSGCPFGDGCHFLHYVPGG 282
>BP067888
Length = 534
Score = 28.9 bits (63), Expect = 1.3
Identities = 23/112 (20%), Positives = 47/112 (41%), Gaps = 2/112 (1%)
Frame = +3
Query: 44 GHPVVFLHGGPGGGTSPSNRRF-FDPEFYRIILFDQRGAGKSTPHACLEHNTTWDLIDDI 102
G V+FLHG P + ++ YR + D RG G + A + + ++ D+
Sbjct: 123 GPVVLFLHGFPELWYTWRHQILSLSALGYRAVAPDLRGYGDTDAPASVTRYACFHMVGDV 302
Query: 103 EKLREHLEIPEWQVFGGSWGSTLALAYSQSHPDKVTGIILRGI-FLLRKKEI 153
L + L + + + WG+ + P+K+ + + F+ R ++
Sbjct: 303 VALIDSLGVDQVFLVAHDWGAMMGWYLCWFRPEKIKAYVCLSVPFMPRNPKV 458
>BP048208
Length = 470
Score = 27.3 bits (59), Expect = 3.7
Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 3/38 (7%)
Frame = -1
Query: 182 GCFVDAYKKRLNSDDIQTQFAAA---RAWTKWEMMTAH 216
GCF + ++ L + I + ++A + WT W M AH
Sbjct: 437 GCFSEGERQELEGEGIGGENSSASRLKEWTTWTMRKAH 324
>AU252197
Length = 350
Score = 27.3 bits (59), Expect = 3.7
Identities = 14/39 (35%), Positives = 20/39 (50%)
Frame = +3
Query: 78 QRGAGKSTPHACLEHNTTWDLIDDIEKLREHLEIPEWQV 116
Q+G T + + DL D EKLR HL+ +W+V
Sbjct: 198 QKGKKDKTSEQLVLYADNHDLSADNEKLRAHLQGMQWRV 314
>AU251527
Length = 390
Score = 26.9 bits (58), Expect = 4.8
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -2
Query: 19 EPYSTGLLKVSDLHTIYWEQSGNP 42
E +ST L+KVS L +I+W +S P
Sbjct: 368 ELFSTKLVKVSILTSIFWYKSSKP 297
>TC19109 similar to UP|Q84LE0 (Q84LE0) Phytocyanin protein, PUP2, partial
(5%)
Length = 479
Score = 26.2 bits (56), Expect = 8.2
Identities = 14/41 (34%), Positives = 23/41 (55%)
Frame = -1
Query: 21 YSTGLLKVSDLHTIYWEQSGNPSGHPVVFLHGGPGGGTSPS 61
+ T ++ DLHT+ W Q+ +P+ P +HG +SPS
Sbjct: 467 FRTCKIRHCDLHTLPWLQNRSPALSP---MHGTLHSPSSPS 354
>TC11665 similar to UP|AAR37673 (AAR37673) Cytochrome c-type biogenesis
protein CcmE, partial (16%)
Length = 469
Score = 26.2 bits (56), Expect = 8.2
Identities = 11/17 (64%), Positives = 12/17 (69%)
Frame = -2
Query: 56 GGTSPSNRRFFDPEFYR 72
GGTSPS RR F P +R
Sbjct: 210 GGTSPSKRRLFPPLTHR 160
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,383,230
Number of Sequences: 28460
Number of extensions: 92601
Number of successful extensions: 389
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 388
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 389
length of query: 333
length of database: 4,897,600
effective HSP length: 91
effective length of query: 242
effective length of database: 2,307,740
effective search space: 558473080
effective search space used: 558473080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0276.3