BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0269b.9
(1085 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15312 UP|RPOD_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'... 1827 0.0
AV779752 31 1.2
TC11776 30 1.6
TC10848 weakly similar to UP|C883_ARATH (O23051) Cytochrome P450... 29 3.5
TC14439 similar to UP|Q9FIC1 (Q9FIC1) Gb|AAF19567.1, partial (49%) 29 4.6
BP048030 28 6.0
TC18902 weakly similar to GB|AAP37786.1|30725528|BT008427 At1g51... 28 6.0
BP041257 28 6.0
>TC15312 UP|RPOD_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain
(PEP) (Plastid-encoded RNA polymerase beta'' subunit)
(RNA polymerase beta'' subunit) , partial (84%)
Length = 3522
Score = 1827 bits (4732), Expect(2) = 0.0
Identities = 905/925 (97%), Positives = 909/925 (97%)
Frame = +3
Query: 159 IYIGSRCIVVRNQDIGIGLINRFINFQTQPIFIRTPFTCRNTSWICRLCYGRSPIHGDLV 218
IYIGSRCIVVRNQDIGIGLINRFINFQTQPIFIRTPFTCRNTSWICRLCYGRSPIHG+LV
Sbjct: 138 IYIGSRCIVVRNQDIGIGLINRFINFQTQPIFIRTPFTCRNTSWICRLCYGRSPIHGNLV 317
Query: 219 ELGEAVGIIAGQSIGEPGTQLTLITFHTGGVFTGGTAEYVRSPSNGKIKFNENSAYPTRT 278
ELGEAVGIIAGQSIGEPGTQLTL TFHTGGVFTGGTAEYVRSPSNGKIKFNENSAYPTRT
Sbjct: 318 ELGEAVGIIAGQSIGEPGTQLTLRTFHTGGVFTGGTAEYVRSPSNGKIKFNENSAYPTRT 497
Query: 279 RHGHPAFLCYIDLYVTIESNDIMHNVIIPPKSFLLVQNDQYVKSEQIIAEIRAGTYTLNL 338
RHGHPAFLCYIDLYVTIESNDIMHNVIIPPKSFLLVQNDQYVKSEQIIAEIRAGTYTLNL
Sbjct: 498 RHGHPAFLCYIDLYVTIESNDIMHNVIIPPKSFLLVQNDQYVKSEQIIAEIRAGTYTLNL 677
Query: 339 KEKIRKHIFSDSEGEMHWSTNIYHVSEFAYSNVHILPKTSHLWILSGNSHKSDTVSLSLL 398
KEK+RKHIFSDSEGEMHWSTNIYHVSEFAYSNVHILPKTSHLWILSGNSHKSDTVSLSLL
Sbjct: 678 KEKVRKHIFSDSEGEMHWSTNIYHVSEFAYSNVHILPKTSHLWILSGNSHKSDTVSLSLL 857
Query: 399 KDQDQMSTHSLPTAKRNTSNFLVSNNQVRLCPDHCHFMHPTISPDTSNLLAKKRRNRFII 458
KDQDQMSTHSLPTAKRNTSNFLVSNNQVRLCPDHCHFMHPTISPDTSNLLAKKRRNRFII
Sbjct: 858 KDQDQMSTHSLPTAKRNTSNFLVSNNQVRLCPDHCHFMHPTISPDTSNLLAKKRRNRFII 1037
Query: 459 PFLFRSIRERNNELMPDISVEIPIDGIIHKNSILAYFDDPQYRTQSSGIAKYKTIGIHSI 518
PFLFRSIRERNNELMPDISVEIPIDGIIHKNSILAYFDDPQYRTQSSGIAKYKTIGIHSI
Sbjct: 1038 PFLFRSIRERNNELMPDISVEIPIDGIIHKNSILAYFDDPQYRTQSSGIAKYKTIGIHSI 1217
Query: 519 FQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPITLNK 578
FQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPITLNK
Sbjct: 1218 FQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPITLNK 1397
Query: 579 KSRVGGLVRVEKNKKKIELKIFSGDIHFPGEIDKISQHSAILIPPEMVKKKILRNQKKKT 638
KSRVGGLVRVEKNKKKIELKIFSGDIHFPGEIDKISQHSAILIPPEMVKKK + KKKT
Sbjct: 1398 KSRVGGLVRVEKNKKKIELKIFSGDIHFPGEIDKISQHSAILIPPEMVKKKNSKESKKKT 1577
Query: 639 NWRYIQWITTTKKKYFVLVRPVILYDIADSINLVKLFPQDLFKEWDNLELKVLNFILYGN 698
NWRYIQWITTTKKKYFVLVRPVILYDIADSINLVKLFPQDLFKEWDNLELKVLNFILYGN
Sbjct: 1578 NWRYIQWITTTKKKYFVLVRPVILYDIADSINLVKLFPQDLFKEWDNLELKVLNFILYGN 1757
Query: 699 GKSIRGILDTSIQLVRTCLVLNWNEDEKSSSIEEALASFVEVSTNGLIRYFLRIDLVKSH 758
GKSIRGILDTSIQLVRTCLVLNWNEDEKSSSIEEALASFVEVSTNGLIRYFLRIDLVKSH
Sbjct: 1758 GKSIRGILDTSIQLVRTCLVLNWNEDEKSSSIEEALASFVEVSTNGLIRYFLRIDLVKSH 1937
Query: 759 ISYIRKRNDPSSSGLISYNESDRININPFFSIYKENIQQSLSQKHGTIRMLLNRNKENRS 818
ISYIRKRNDPSSSGLISYNESDRININPFFSIYKENIQQSLSQKHGTIRMLLNRNKENRS
Sbjct: 1938 ISYIRKRNDPSSSGLISYNESDRININPFFSIYKENIQQSLSQKHGTIRMLLNRNKENRS 2117
Query: 819 FIILSSSNCFQMGPFNNVKYHNGIKEEINQFKRNHKIPIKISLGPLGVAPQIANFFSFYH 878
FIILSSSNCFQMGPFNNVKYHNGIKEEINQFKRNHKIPIKISLGPLGVAPQIANFFSFYH
Sbjct: 2118 FIILSSSNCFQMGPFNNVKYHNGIKEEINQFKRNHKIPIKISLGPLGVAPQIANFFSFYH 2297
Query: 879 LITHNKISSIKKNLQLNKFKETFQVIKYYLMDENERIYKPDLYNNIILNPFHLNWDFIHP 938
LITHNKISSIKKNLQLNKFKETFQVIKYYLMDENERIYKPDLYNNIILNPFHLNWDFIHP
Sbjct: 2298 LITHNKISSIKKNLQLNKFKETFQVIKYYLMDENERIYKPDLYNNIILNPFHLNWDFIHP 2477
Query: 939 NYCEKTFPIISLGQFICENVCIVQTKNGPNLKSGQVITVQMDFVGIRLANPYLATPGATI 998
NYCEKTFPIISLGQFICENVCIVQTKNGPNLKSGQVITVQMDFVGIRLANPYLATPGATI
Sbjct: 2478 NYCEKTFPIISLGQFICENVCIVQTKNGPNLKSGQVITVQMDFVGIRLANPYLATPGATI 2657
Query: 999 HGHYGEMLYEGDILVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISMNLEKRIDAWNER 1058
HGHYGEMLYEGDILVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISMNLEKRIDAWNER
Sbjct: 2658 HGHYGEMLYEGDILVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISMNLEKRIDAWNER 2837
Query: 1059 ITGILGIPWSRKISMSRKLLQDNIS 1083
ITGILGIPW I + Q IS
Sbjct: 2838 ITGILGIPWRFLIGAELTIAQSRIS 2912
Score = 107 bits (267), Expect(2) = 0.0
Identities = 48/48 (100%), Positives = 48/48 (100%)
Frame = +2
Query: 113 *SGSTHCCTPNRLRYRSRDFCKYSE*NDVRKNFDTNINWSCISRRYIY 160
*SGSTHCCTPNRLRYRSRDFCKYSE*NDVRKNFDTNINWSCISRRYIY
Sbjct: 2 *SGSTHCCTPNRLRYRSRDFCKYSE*NDVRKNFDTNINWSCISRRYIY 145
>AV779752
Length = 593
Score = 30.8 bits (68), Expect = 1.2
Identities = 17/64 (26%), Positives = 29/64 (44%), Gaps = 2/64 (3%)
Frame = +3
Query: 141 VRKNFDTNINWSCISRRYIYIGSR--CIVVRNQDIGIGLINRFINFQTQPIFIRTPFTCR 198
++ ++D + W C Y + S C ++ N G+GL F NF+ I +T T
Sbjct: 297 MKLHYDVWVEWVCFFLVYGFTSSEWVCHLISNFLSGMGLSLSFFNFKRNQIEGKTRMTSE 476
Query: 199 NTSW 202
+ W
Sbjct: 477 DDDW 488
>TC11776
Length = 612
Score = 30.4 bits (67), Expect = 1.6
Identities = 22/71 (30%), Positives = 35/71 (48%)
Frame = -3
Query: 725 EKSSSIEEALASFVEVSTNGLIRYFLRIDLVKSHISYIRKRNDPSSSGLISYNESDRINI 784
EK S + +++VEV T R FLR++ H+ Y+ K S + +YN + +
Sbjct: 607 EKISCMRVNRSTYVEVITV*ESRIFLRLESHSLHMEYVFKLKGNSRA---TYNSKRKQ*L 437
Query: 785 NPFFSIYKENI 795
N I K+NI
Sbjct: 436 NQNICILKQNI 404
>TC10848 weakly similar to UP|C883_ARATH (O23051) Cytochrome P450 88A3 ,
partial (16%)
Length = 889
Score = 29.3 bits (64), Expect = 3.5
Identities = 15/60 (25%), Positives = 31/60 (51%)
Frame = -3
Query: 262 SNGKIKFNENSAYPTRTRHGHPAFLCYIDLYVTIESNDIMHNVIIPPKSFLLVQNDQYVK 321
++ KI +S P + P+ + ++D+Y + S+ ++N +I S +L+ YVK
Sbjct: 194 TSSKICLQRHSMSPKWQQTPKPSIVPFLDVYRLLNSSLSLYNKLISDVSLMLISISLYVK 15
>TC14439 similar to UP|Q9FIC1 (Q9FIC1) Gb|AAF19567.1, partial (49%)
Length = 2120
Score = 28.9 bits (63), Expect = 4.6
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 188 PIFIRTPFTCRNTSWICRL 206
P+F+R FTC+ W CR+
Sbjct: 1559 PLFVRANFTCKKRLWGCRM 1615
>BP048030
Length = 323
Score = 28.5 bits (62), Expect = 6.0
Identities = 18/48 (37%), Positives = 22/48 (45%)
Frame = +2
Query: 248 GVFTGGTAEYVRSPSNGKIKFNENSAYPTRTRHGHPAFLCYIDLYVTI 295
GV +G A RSPSN F ++ T P F C I LY +I
Sbjct: 59 GVPSGLVASQTRSPSNPTTSFISSANSLIVTSFPLPTFKCSIPLYFSI 202
>TC18902 weakly similar to GB|AAP37786.1|30725528|BT008427 At1g51560
{Arabidopsis thaliana;}, partial (11%)
Length = 575
Score = 28.5 bits (62), Expect = 6.0
Identities = 8/18 (44%), Positives = 13/18 (71%)
Frame = -2
Query: 349 DSEGEMHWSTNIYHVSEF 366
D+EG++HW N++H F
Sbjct: 574 DNEGDLHWYNNLFHFRNF 521
>BP041257
Length = 478
Score = 28.5 bits (62), Expect = 6.0
Identities = 14/43 (32%), Positives = 21/43 (48%)
Frame = -2
Query: 845 EINQFKRNHKIPIKISLGPLGVAPQIANFFSFYHLITHNKISS 887
E+ +FK +H I SL P ++ + S H +TH I S
Sbjct: 420 ELGEFKTSHIFLIHFSLFPFPISHHLLELLSKPHSLTHRLIHS 292
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.327 0.142 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,589,382
Number of Sequences: 28460
Number of extensions: 321896
Number of successful extensions: 2114
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 2080
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2110
length of query: 1085
length of database: 4,897,600
effective HSP length: 100
effective length of query: 985
effective length of database: 2,051,600
effective search space: 2020826000
effective search space used: 2020826000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0269b.9