BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0262.11
(581 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP030435 41 5e-04
BI418821 39 0.002
BG662122 39 0.002
TC15506 similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich prote... 39 0.003
TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1... 36 0.020
BP054244 34 0.075
TC8946 homologue to GB|AAM66972.1|21617922|AY088650 pyridoxine b... 34 0.075
TC15182 similar to UP|Q9ZQW9 (Q9ZQW9) U2 snRNP auxiliary factor,... 33 0.13
TC14532 similar to UP|Q9FYA8 (Q9FYA8) SC35-like splicing factor ... 32 0.28
TC10006 similar to UP|P93396 (P93396) Transformer-SR ribonucleop... 32 0.28
AV410018 32 0.37
AW720177 32 0.37
TC18242 weakly similar to UP|O70495 (O70495) Plenty-of-prolines-... 32 0.37
TC14308 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial ... 32 0.37
TC19122 similar to UP|Q9LXI5 (Q9LXI5) Zinc finger-like protein, ... 31 0.63
TC14845 31 0.63
TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, pa... 30 0.83
TC10721 similar to UP|MTD_MEDSA (O82515) Probable mannitol dehyd... 30 1.1
BI420843 30 1.1
TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Ar... 30 1.1
>BP030435
Length = 533
Score = 41.2 bits (95), Expect = 5e-04
Identities = 33/110 (30%), Positives = 49/110 (44%), Gaps = 16/110 (14%)
Frame = +1
Query: 405 VGSIAGGFAAGGHTSNSRK------SSI*VIMSAMGRPRPRHSEPSRQKVTIWFSDDDYD 458
V +IAGGF GG TS +RK +++ ++ P I FS DD+
Sbjct: 232 VHTIAGGFGGGGITSAARKRYARAVNTVTEVLFGFSHP------------VITFSSDDFI 375
Query: 459 TYTGKEDDPIVI----------EALIGNGSSADVMFYDAYKKLHLSVKDL 498
D+PIVI L+ GSSAD+++ + +L L+ DL
Sbjct: 376 RIKPHLDNPIVILLRVNQLNVQRVLLDQGSSADIIYGGVFDRLGLNEADL 525
>BI418821
Length = 614
Score = 39.3 bits (90), Expect = 0.002
Identities = 26/63 (41%), Positives = 29/63 (45%), Gaps = 5/63 (7%)
Frame = +1
Query: 342 RRSPTPPRQRA-RTPPRHR----TDDRYCRAPQNGRSPDRRRSFDRGRIPDRHGRDEVIR 396
RR P PPR+R+ R RHR R C PQ RSP + F RI GR E R
Sbjct: 184 RRLPNPPRRRSRRVLHRHRRQRQDQSRRCHRPQRRRSPAHSQGFRPARIRRMEGRREA*R 363
Query: 397 HHR 399
R
Sbjct: 364 WRR 372
>BG662122
Length = 386
Score = 38.9 bits (89), Expect = 0.002
Identities = 28/98 (28%), Positives = 52/98 (52%), Gaps = 10/98 (10%)
Frame = +2
Query: 238 RRKNQDEKKP-----KRKRYENYAPLNSLSQILRERASTDLRDR-----PPPMLTKGDKL 287
RR+ D ++P R+R E+ L+ IL++ + + + PPP +G +
Sbjct: 29 RREALDRRRP*QPMGSRRREEDMELNAHLTDILQDVKAAHMVGKSGQS*PPPR--RG--I 196
Query: 288 DSKRFCEFHDSPDNNTDECLNLKDKVK*LIRAGRLSKY 325
D+ + CE+ S ++ D+C LK +++ LI+ GRL +Y
Sbjct: 197 DTTK*CEYRRSVVHDIDDCFTLKREIEKLIKMGRLKQY 310
>TC15506 similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich protein, partial
(24%)
Length = 677
Score = 38.5 bits (88), Expect = 0.003
Identities = 30/65 (46%), Positives = 34/65 (52%), Gaps = 11/65 (16%)
Frame = +2
Query: 338 SPTSRRSPTPPRQRARTPPRHRTDDRYCRAP---QNGRSPDRR------RSFD--RGRIP 386
SP RRSP PP +R R+PP R R+P + RSP RR RSF RGR P
Sbjct: 26 SPGRRRSPPPPPRR-RSPPPRRARSPPRRSPIGRRRSRSPIRRPARSNSRSFSPRRGRPP 202
Query: 387 DRHGR 391
R GR
Sbjct: 203 VRRGR 217
>TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1_4
{Arabidopsis thaliana;}, partial (55%)
Length = 1276
Score = 35.8 bits (81), Expect = 0.020
Identities = 32/103 (31%), Positives = 43/103 (41%), Gaps = 4/103 (3%)
Frame = +2
Query: 351 RARTPPRHRTDDRYCRAPQNGRSPDRRRSFDRGRIPDRH-GRDEVIRHHRMNQISVGSIA 409
R+R+P R DD ++ R R RS+D+ DRH GRD R R S
Sbjct: 437 RSRSPRRRHRDDHRSSRDRDYRRRSRSRSYDKYERGDRHRGRDRDYR-RRSRSRSASLDY 613
Query: 410 GGFAAGGH---TSNSRKSSI*VIMSAMGRPRPRHSEPSRQKVT 449
G G + S SR S+ A P PR S ++ V+
Sbjct: 614 KGRGRGRYDDEPSRSRSRSVDSRSPARRSPSPRRSPSPKRSVS 742
Score = 34.3 bits (77), Expect = 0.057
Identities = 24/58 (41%), Positives = 31/58 (53%), Gaps = 7/58 (12%)
Frame = +2
Query: 334 PLPRSPTSRRSPTPPR----QRARTP---PRHRTDDRYCRAPQNGRSPDRRRSFDRGR 384
P RSP+ RRSP+P R +R+ +P PR + D R +GRSP R RGR
Sbjct: 686 PARRSPSPRRSPSPKRSVSPKRSTSPRKSPRGESPDNRSR---DGRSPTPRSVSPRGR 850
>BP054244
Length = 465
Score = 33.9 bits (76), Expect = 0.075
Identities = 25/61 (40%), Positives = 35/61 (56%), Gaps = 1/61 (1%)
Frame = -1
Query: 141 KESLKTFLNRFNKEAGDIHYLLPQVRLVLVR*ALHPG-PFLTSLDGKKAKTVEEFQARSE 199
KESL+ FL +F KEA + L +VRL L+ A+ G F TS ++ ++EFQ S
Sbjct: 438 KESLRDFLTKFYKEATLVTSLDLKVRLHLLCEAIRMGTSFYTSSAXTPSQDMDEFQRXSI 259
Query: 200 K 200
K
Sbjct: 258 K 256
>TC8946 homologue to GB|AAM66972.1|21617922|AY088650 pyridoxine
biosynthesis protein-like {Arabidopsis thaliana;} ,
partial (45%)
Length = 503
Score = 33.9 bits (76), Expect = 0.075
Identities = 25/69 (36%), Positives = 31/69 (44%), Gaps = 6/69 (8%)
Frame = +3
Query: 336 PRSPTSRRSPTPPRQRARTPPRHRT------DDRYCRAPQNGRSPDRRRSFDRGRIPDRH 389
PRS RR RQR +P R R+ R C P+ P RRRS +R P H
Sbjct: 168 PRSDAPRRRHHGRRQRPTSPHRRRSRRLRRHGPRAC--PRRHPRPRRRRSHER---PSAH 332
Query: 390 GRDEVIRHH 398
++ RHH
Sbjct: 333 QGNQTSRHH 359
>TC15182 similar to UP|Q9ZQW9 (Q9ZQW9) U2 snRNP auxiliary factor, small
subunit, partial (12%)
Length = 642
Score = 33.1 bits (74), Expect = 0.13
Identities = 21/44 (47%), Positives = 24/44 (53%), Gaps = 1/44 (2%)
Frame = +2
Query: 349 RQRARTPPRHRTDDRYCRAPQ-NGRSPDRRRSFDRGRIPDRHGR 391
R R+R+PPR+R Y PQ GR RR FDR RHGR
Sbjct: 71 RSRSRSPPRNR---NYEERPQFGGRGGSGRRDFDRSH--GRHGR 187
>TC14532 similar to UP|Q9FYA8 (Q9FYA8) SC35-like splicing factor SCL30a, 30a
kD, partial (71%)
Length = 1073
Score = 32.0 bits (71), Expect = 0.28
Identities = 20/49 (40%), Positives = 26/49 (52%), Gaps = 5/49 (10%)
Frame = +3
Query: 341 SRRSPTP----PRQRARTPPRHRTDDRYCRAPQNGRS-PDRRRSFDRGR 384
+R+ PT R R R+ R R+ RY R+P+ RS P R RS R R
Sbjct: 405 NRKKPTEMRHRERSRGRSYDRRRSPPRYSRSPRYSRSPPPRHRSRSRSR 551
Score = 26.9 bits (58), Expect = 9.1
Identities = 17/42 (40%), Positives = 20/42 (47%)
Frame = +3
Query: 337 RSPTSRRSPTPPRQRARTPPRHRTDDRYCRAPQNGRSPDRRR 378
RSP RSP PPR R+R+ R R SP+ RR
Sbjct: 492 RSPRYSRSP-PPRHRSRSRSRDYYSPPPKRRDSRSVSPEERR 614
>TC10006 similar to UP|P93396 (P93396) Transformer-SR ribonucleoprotein
(Fragment), partial (30%)
Length = 453
Score = 32.0 bits (71), Expect = 0.28
Identities = 26/89 (29%), Positives = 42/89 (46%), Gaps = 2/89 (2%)
Frame = +3
Query: 334 PLPRSPTSRRSPTPPRQRARTP-PRHRTDDRYCRAPQNGRSPDRRRSFDRGRIP-DRHGR 391
P P+S + R PR R+R+P PR R+ + P RS R R R R P DR+G
Sbjct: 192 PRPKSRSPRAKSRSPRPRSRSPRPRSRSRSLEKQRP---RSRSRSRGRSRSRSPEDRNGG 362
Query: 392 DEVIRHHRMNQISVGSIAGGFAAGGHTSN 420
+ + ++++ + FA G ++
Sbjct: 363 NTLYVTGLSSRVTERDLEEHFAKEGKVAS 449
Score = 30.8 bits (68), Expect = 0.63
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +3
Query: 334 PLPRSPTSRRSPTPPRQRARTP-PRHRTDDRYCRAPQNGRSPDRRRSFDRGRIPDRHGRD 392
P P+S + R PR ++R+P PR R+ R+ + R RS RGR R D
Sbjct: 171 PRPKSRSPRPKSRSPRAKSRSPRPRSRSPRPRSRSRSLEKQRPRSRSRSRGRSRSRSPED 350
>AV410018
Length = 429
Score = 31.6 bits (70), Expect = 0.37
Identities = 20/51 (39%), Positives = 23/51 (44%), Gaps = 3/51 (5%)
Frame = -2
Query: 336 PRSPTSRRSPTPPRQRARTPPRHRTDDRYCRAP---QNGRSPDRRRSFDRG 383
P PT+R + R R P HR R R P Q SP RRR+ RG
Sbjct: 335 PAWPTARAGSSVTACRRRLPRGHRAPPRRSRTP*RRQGSHSPPRRRAPCRG 183
>AW720177
Length = 505
Score = 31.6 bits (70), Expect = 0.37
Identities = 14/26 (53%), Positives = 17/26 (64%)
Frame = +2
Query: 333 LPLPRSPTSRRSPTPPRQRARTPPRH 358
+PLP P SR +P P +R R PPRH
Sbjct: 332 VPLPHPPPSRXAPNP--KRPRLPPRH 403
>TC18242 weakly similar to UP|O70495 (O70495) Plenty-of-prolines-101,
partial (5%)
Length = 490
Score = 31.6 bits (70), Expect = 0.37
Identities = 19/53 (35%), Positives = 29/53 (53%), Gaps = 1/53 (1%)
Frame = +3
Query: 337 RSPTSRRSPTPPRQRARTPPRHRTDDRYCRAPQNGR-SPDRRRSFDRGRIPDR 388
RS ++ RSP +R+R+P R R+ R+ + ++G SP R S R R R
Sbjct: 18 RSRSNSRSPPRRYRRSRSPSRSRSSRRHYSSSRSGSPSPSRTHSRSRSRSRSR 176
>TC14308 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial (96%)
Length = 764
Score = 31.6 bits (70), Expect = 0.37
Identities = 23/60 (38%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = +1
Query: 339 PTSRRSPTPPRQRARTPPRHRTDDRYCRAPQNGRSPDRRRSFDRGR--IPDRHGRDEVIR 396
P RSP R R+PPR R+ R P+ RSP RSF R +P+ R E R
Sbjct: 82 PRQTRSP-----RRRSPPRRRSRRWLRRLPRRRRSPRPGRSFPRRAQPLPETRRRREARR 246
>TC19122 similar to UP|Q9LXI5 (Q9LXI5) Zinc finger-like protein, partial
(58%)
Length = 737
Score = 30.8 bits (68), Expect = 0.63
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = -1
Query: 336 PRSPTSRRSPTPPRQRARTPPRHRTDDRY-CRAPQNGRSPDRRRSFDR 382
P +P +RR+P P R H ++R CR + R DRRR R
Sbjct: 512 PHAPPARRAPARPLLLRRRRRSHNNNNRLRCRRRRRARLRDRRRQRQR 369
>TC14845
Length = 1110
Score = 30.8 bits (68), Expect = 0.63
Identities = 22/70 (31%), Positives = 33/70 (46%), Gaps = 3/70 (4%)
Frame = -3
Query: 336 PRSPTSRRSPTPPRQRARTPPRHR---TDDRYCRAPQNGRSPDRRRSFDRGRIPDRHGRD 392
P P +RRS T +ART P H+ ++ R+ A +NG R+ GR GR
Sbjct: 553 PWEPDARRSKTESTNQARTHPEHQHLPSESRHRAATENGGG----RATASGRETACCGRR 386
Query: 393 EVIRHHRMNQ 402
++R R +
Sbjct: 385 GMLRRRRKRE 356
>TC19515 similar to UP|Q9YGK0 (Q9YGK0) Vitellogenin precursor, partial (3%)
Length = 504
Score = 30.4 bits (67), Expect = 0.83
Identities = 24/81 (29%), Positives = 35/81 (42%), Gaps = 3/81 (3%)
Frame = +3
Query: 433 MGRPRPRHSEPSRQKVTIWFSDDDYDTYTGKEDDPIVIEALIGNGSSADVMFYDAY---K 489
+GR RP + +DD D G ++DP++ L G S +D M +D Y
Sbjct: 36 IGRCRPTDDDSDPDDAESEDEEDDDDDDDGDDEDPLLGPGL-GRESDSDDMSHDDYDSAS 212
Query: 490 KLHLSVKDLLLYDHDLVGFTG 510
+ D +L D D G TG
Sbjct: 213 DIDDEDADFILDDLDFDGGTG 275
>TC10721 similar to UP|MTD_MEDSA (O82515) Probable mannitol dehydrogenase
(NAD-dependent mannitol dehydrogenase) , complete
Length = 1297
Score = 30.0 bits (66), Expect = 1.1
Identities = 12/27 (44%), Positives = 20/27 (73%)
Frame = +1
Query: 239 RKNQDEKKPKRKRYENYAPLNSLSQIL 265
R+ + EKK K+K + N+ +NSLS++L
Sbjct: 37 RRTEKEKKKKKKAWPNHLKVNSLSKLL 117
>BI420843
Length = 377
Score = 30.0 bits (66), Expect = 1.1
Identities = 22/53 (41%), Positives = 23/53 (42%), Gaps = 3/53 (5%)
Frame = -3
Query: 337 RSPTSRRSPTPPRQRARTPP---RHRTDDRYCRAPQNGRSPDRRRSFDRGRIP 386
RSP SR S PP RTP RHR D AP S RR F + P
Sbjct: 285 RSPPSRSSEPPPEHHTRTPAALVRHRRGDSPPPAPP--PSVQTRRCFVKCSAP 133
>TC15958 similar to GB|AAP37838.1|30725632|BT008479 At4g24220 {Arabidopsis
thaliana;}, partial (52%)
Length = 800
Score = 30.0 bits (66), Expect = 1.1
Identities = 43/156 (27%), Positives = 56/156 (35%), Gaps = 14/156 (8%)
Frame = +1
Query: 233 RIRESRRKNQDEKKPKRKRYENYAPLNSLSQILRERASTDLRDRPPPMLTKGDKLDSKRF 292
R ++ RR + + P+R PL SL + R ST L R P L L S+R
Sbjct: 277 RRQQPRRDSPSSRHPRR-------PLESLRR--RPPPSTLLERRSPNRLHPVRHLRSRRH 429
Query: 293 CEFHDSPDNNTDECLNLKDKVK*LIRAGRLSKYVALSYGDLPLPRSPTSRRSPTPPRQRA 352
P L L D+ I G+L R +R P QRA
Sbjct: 430 PSQALRPHRRHPHLLRLLDQPLHRI-------------GELRGQRRHAQKRPPRRHPQRA 570
Query: 353 RTPPRH--------------RTDDRYCRAPQNGRSP 374
++PPR R DR RA +G SP
Sbjct: 571 QSPPRDAPNRL*ALRRPFRARRQDRVPRAAVHGGSP 678
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.142 0.435
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,012,928
Number of Sequences: 28460
Number of extensions: 155323
Number of successful extensions: 1745
Number of sequences better than 10.0: 93
Number of HSP's better than 10.0 without gapping: 1571
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1711
length of query: 581
length of database: 4,897,600
effective HSP length: 95
effective length of query: 486
effective length of database: 2,193,900
effective search space: 1066235400
effective search space used: 1066235400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0262.11