BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0260.2
(694 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780009 308 1e-84
BP075943 189 1e-48
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 119 2e-27
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 117 8e-27
BP066094 97 8e-23
AV410378 101 3e-22
AV424544 101 5e-22
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 89 2e-18
BP084332 84 1e-16
BP043850 74 8e-14
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 69 3e-12
AU088980 65 3e-11
AV411594 65 5e-11
BP057233 64 6e-11
AV766665 56 2e-08
TC10484 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragm... 37 2e-06
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 48 5e-06
AV769206 47 1e-05
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 45 5e-05
BP084259 44 9e-05
>AV780009
Length = 529
Score = 308 bits (790), Expect = 1e-84
Identities = 150/171 (87%), Positives = 155/171 (89%)
Frame = -1
Query: 514 QAATRVLRYVKGAPGQGLFFSATASLKLQAYSDSDWAGCPDTRRSINGYSIFLGSSLISW 573
QAATRVLRYVKGAP QGLFFSA + LKLQAYSDSDWAGCPDTRRS+ GYSIFLG+SLISW
Sbjct: 529 QAATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISW 350
Query: 574 RTKKQTTVSRSSSEAEYRALAAIVCEVQWLSYLFQFLHLRVPFPVPLFCDNQSAIHIAQN 633
RTKKQTTVSRSSSEAEYRALAA VCEVQWLSYLFQFL L VP PVPLFCDNQSA+HIA N
Sbjct: 349 RTKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHN 170
Query: 634 PTFHERTKHIELDCHVVRTKLQAGLIHLLPVSTRDQLADIFTKPLPPSLFP 684
PTFHERTKHIELDCHVVR KLQAGLIHLLP+ST QLADIFTKP P FP
Sbjct: 169 PTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKPCPHLCFP 17
>BP075943
Length = 547
Score = 189 bits (481), Expect = 1e-48
Identities = 98/168 (58%), Positives = 124/168 (73%), Gaps = 1/168 (0%)
Frame = -3
Query: 372 TSTSITALLLYVDDVLLTGNSFEEIQAVKSFLHSQFRIKDLGDLKFFLGLEVARSAKGIC 431
+S S TALLLYVDDVLL GN EIQ VKS LH QFRIKDLG+ K+FLGLE+ARS GI
Sbjct: 542 SSGSFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIV 363
Query: 432 LTQRKYALELLTDAGLLGCKP-VRTPMDSTLHLSQTGGTLLDDPTSYRRLVGRLLYLTTT 490
L QRKYAL+L++D+G G +P + ++ L GT L D SYRR+VGRLLYL TT
Sbjct: 362 LNQRKYALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTT 183
Query: 491 RPDLNYVVQQLSQYLASPTDLHQQAATRVLRYVKGAPGQGLFFSATAS 538
RPD+ + V QLSQ+L++PTD+H+Q T L+Y+ G+PG GLF+ A++S
Sbjct: 182 RPDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSS 39
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 119 bits (297), Expect = 2e-27
Identities = 58/96 (60%), Positives = 70/96 (72%), Gaps = 2/96 (2%)
Frame = +1
Query: 601 QWLSYLFQFLHLRVPFPVP--LFCDNQSAIHIAQNPTFHERTKHIELDCHVVRTKLQAGL 658
QWL+YL Q L+VPF P ++CDN SA HIA NP FHERTKHIE+DCH+VR ++Q GL
Sbjct: 1 QWLTYLLQ--DLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGL 174
Query: 659 IHLLPVSTRDQLADIFTKPLPPSLFPTFVLKLGLYN 694
IHLLP+S+ + LADI+TK L P F KLGL N
Sbjct: 175 IHLLPISSSEPLADIYTKALSPQNFHQICAKLGLIN 282
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 117 bits (292), Expect = 8e-27
Identities = 60/99 (60%), Positives = 71/99 (71%), Gaps = 2/99 (2%)
Frame = +1
Query: 598 CEVQWLSYLFQFLHLRVPFPVP--LFCDNQSAIHIAQNPTFHERTKHIELDCHVVRTKLQ 655
CE+ WL+Y+ Q LRVPF P L+CD+QSA HIA N FHERTKH+++DCHVVR KLQ
Sbjct: 1 CEL*WLTYILQ--DLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQ 174
Query: 656 AGLIHLLPVSTRDQLADIFTKPLPPSLFPTFVLKLGLYN 694
A L HLLP+S+ DQ ADI TKPL F V KLG+ N
Sbjct: 175 AKLFHLLPISSVDQTADILTKPLESGPFSHLVSKLGVLN 291
>BP066094
Length = 532
Score = 97.4 bits (241), Expect(2) = 8e-23
Identities = 50/149 (33%), Positives = 85/149 (56%), Gaps = 1/149 (0%)
Frame = +2
Query: 275 AKTTTLRLLLALAASQGWFLHQLDVDNAFLHGTLDEEIYMRLPPGVSSPR-PNQVCLLQK 333
+KT +RLL++ + + LHQ++V +AFL+G + EE+Y+ PPG + + + L+K
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 334 SLYGLKQASRQWYNTLVAALKSLGYEQCPHDHTLLLKRTSTSITALLLYVDDVLLTGNSF 393
SLYGLKQA R WY L + L + D TL K I + +YVDD++ +
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 394 EEIQAVKSFLHSQFRIKDLGDLKFFLGLE 422
+ + ++F ++ +G+LK+FLG++
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 26.9 bits (58), Expect(2) = 8e-23
Identities = 10/18 (55%), Positives = 15/18 (82%)
Frame = +3
Query: 259 YTQVEGLDYLDTFSPVAK 276
Y+Q G+DY +TF+PVA+
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
>AV410378
Length = 358
Score = 101 bits (252), Expect = 3e-22
Identities = 57/123 (46%), Positives = 76/123 (61%), Gaps = 3/123 (2%)
Frame = +1
Query: 109 PQVPEVLPVPQTAT--RHSTRSRRVPSHFHDYQLALPSSIAALNSSSKV-SSTGIKHPLS 165
P E L P+ R S R RR PS+ DY L AA + SKV SS GI +PLS
Sbjct: 1 PLPSETLSNPEEGNVQRISNRVRRPPSYLQDYHCTL----AASTTVSKVPSSAGISYPLS 168
Query: 166 SVLSYTNLSPKHYAFTTNVSSTVEPTTYTQAAQCKEWRDAMDKELEALSRNNTWLIVDLP 225
V+SY L+P + AF N+++ EPT Y++A + + WR AMD+E+EAL RN+TW++VD P
Sbjct: 169 KVISYHKLTPSYRAFIMNITTVSEPTRYSEAVKHECWRKAMDQEIEALERNHTWILVDKP 348
Query: 226 PTK 228
P K
Sbjct: 349 PDK 357
>AV424544
Length = 276
Score = 101 bits (251), Expect = 5e-22
Identities = 52/89 (58%), Positives = 63/89 (70%)
Frame = +3
Query: 349 LVAALKSLGYEQCPHDHTLLLKRTSTSITALLLYVDDVLLTGNSFEEIQAVKSFLHSQFR 408
L + L LGY Q HDH+L K S T +L+YVDD++L GN EIQ VK+ L QFR
Sbjct: 9 LSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQFR 188
Query: 409 IKDLGDLKFFLGLEVARSAKGICLTQRKY 437
IKDLG LK+FLGLEVARS+ G+ L+QRKY
Sbjct: 189 IKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 89.0 bits (219), Expect = 2e-18
Identities = 38/81 (46%), Positives = 57/81 (69%)
Frame = +3
Query: 598 CEVQWLSYLFQFLHLRVPFPVPLFCDNQSAIHIAQNPTFHERTKHIELDCHVVRTKLQAG 657
CE WL+Y L + V ++CDN+SA+H+A N FH+RT++IE+DCH+V K+ G
Sbjct: 18 CEALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFG 197
Query: 658 LIHLLPVSTRDQLADIFTKPL 678
++HLL V + DQ+AD+FTK +
Sbjct: 198 ILHLLHVPSSDQVADVFTKTI 260
>BP084332
Length = 368
Score = 83.6 bits (205), Expect = 1e-16
Identities = 43/112 (38%), Positives = 69/112 (61%)
Frame = +2
Query: 578 QTTVSRSSSEAEYRALAAIVCEVQWLSYLFQFLHLRVPFPVPLFCDNQSAIHIAQNPTFH 637
Q+T++ S++EAEY + A ++ W+ + + + + +P++CDN +AI +++NP H
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQI-LESNIPIYCDNTAAISLSKNPILH 208
Query: 638 ERTKHIELDCHVVRTKLQAGLIHLLPVSTRDQLADIFTKPLPPSLFPTFVLK 689
R KHIE+ H +R +Q G++ L V T Q ADIFTKPL F F+LK
Sbjct: 209 SRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRF-NFILK 361
>BP043850
Length = 515
Score = 73.9 bits (180), Expect = 8e-14
Identities = 44/94 (46%), Positives = 50/94 (52%)
Frame = -1
Query: 599 EVQWLSYLFQFLHLRVPFPVPLFCDNQSAIHIAQNPTFHERTKHIELDCHVVRTKLQAGL 658
E+ WL L L P PL DN SAI IA NP +HE T+HIE+DCH VR +
Sbjct: 512 EIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRRV 333
Query: 659 IHLLPVSTRDQLADIFTKPLPPSLFPTFVLKLGL 692
I L VST Q+ADI TK L V KL L
Sbjct: 332 ITLPHVSTSVQIADILTKSLTRQRHNFLVSKLML 231
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 68.9 bits (167), Expect = 3e-12
Identities = 40/117 (34%), Positives = 62/117 (52%), Gaps = 3/117 (2%)
Frame = +2
Query: 353 LKSLGYEQCPHDHTLLLKR-TSTSITALLLYVDDVLLTGNSFEEIQAVKSFLHSQFRIKD 411
+ SLGY + DH KR LLLYVDD+L+ G + + +Q +K+ L +F +KD
Sbjct: 14 IMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDMKD 193
Query: 412 LGDLKFFLGLEVARSAKG--ICLTQRKYALELLTDAGLLGCKPVRTPMDSTLHLSQT 466
LG LG+++ R K I L+Q+ Y ++L + C P+ TP+ LS +
Sbjct: 194 LGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSS 364
>AU088980
Length = 360
Score = 65.5 bits (158), Expect = 3e-11
Identities = 26/49 (53%), Positives = 38/49 (77%)
Frame = +2
Query: 7 LKTFGSLCYASTLNRNRTKLDPRAQKCVFIGYRHGTKGFLLFDIKSREV 55
LK FG+LCY+STL N KLDPRA+KCVF+G++ GT G+++ D+ + +
Sbjct: 200 LKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPTTXI 346
>AV411594
Length = 244
Score = 64.7 bits (156), Expect = 5e-11
Identities = 32/65 (49%), Positives = 43/65 (65%)
Frame = +2
Query: 418 FLGLEVARSAKGICLTQRKYALELLTDAGLLGCKPVRTPMDSTLHLSQTGGTLLDDPTSY 477
FL +A+S KGI L++RKYAL+LL D +GCKP P+D ++ L+ T G LL D T Y
Sbjct: 50 FLRT*IAKSKKGILLSRRKYALQLLDDTRNMGCKPPPFPLDPSIKLNSTDGELLSDVTLY 229
Query: 478 RRLVG 482
R +G
Sbjct: 230 IRFIG 244
Score = 28.1 bits (61), Expect = 4.9
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = +3
Query: 403 LHSQFRIKDLGDLKFFLGLEVARSAKGIC 431
L F++K L D+K+FLGLE K C
Sbjct: 6 LAKSFKLKVLDDMKYFLGLE*PSLRKEFC 92
>BP057233
Length = 473
Score = 64.3 bits (155), Expect = 6e-11
Identities = 34/81 (41%), Positives = 47/81 (57%)
Frame = -2
Query: 612 LRVPFPVPLFCDNQSAIHIAQNPTFHERTKHIELDCHVVRTKLQAGLIHLLPVSTRDQLA 671
L +P L+CDN SA +A NP H R+KHIE+D H +R ++ + + V T DQ+A
Sbjct: 469 LPMPRKPILWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIA 290
Query: 672 DIFTKPLPPSLFPTFVLKLGL 692
D TKPL + F KLG+
Sbjct: 289 DCLTKPLSHTRFSQLRDKLGV 227
>AV766665
Length = 601
Score = 55.8 bits (133), Expect = 2e-08
Identities = 27/76 (35%), Positives = 43/76 (56%)
Frame = +3
Query: 517 TRVLRYVKGAPGQGLFFSATASLKLQAYSDSDWAGCPDTRRSINGYSIFLGSSLISWRTK 576
TR+ Y+K +G F + ++ +D+ G R S GY +FL +L++WR+K
Sbjct: 369 TRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTWRSK 548
Query: 577 KQTTVSRSSSEAEYRA 592
+Q ++RSS EAE RA
Sbjct: 549 QQNIIARSSGEAELRA 596
>TC10484 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragment),
partial (20%)
Length = 479
Score = 37.0 bits (84), Expect(2) = 2e-06
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +3
Query: 602 WLSYLFQFLHLRVPFPVPLFCDNQSAIHIAQNPT 635
W+ + + + P+PL+CDNQ+A+HI+ NP+
Sbjct: 3 WVGQILSEMGIERISPMPLWCDNQAALHISSNPS 104
Score = 31.6 bits (70), Expect(2) = 2e-06
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = +2
Query: 635 TFHERTKHIELDCH 648
+ HERTKHIE+DCH
Sbjct: 107 SLHERTKHIEVDCH 148
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 48.1 bits (113), Expect = 5e-06
Identities = 41/144 (28%), Positives = 60/144 (41%), Gaps = 4/144 (2%)
Frame = -1
Query: 434 QRKYALELLTDAGLLGCKPVRTPMDST-LHLSQTGGTLLDDPTSYRRLVGRLLYLTTTRP 492
Q+ Y ++L + K + T + + + G D T Y+ L+G + YL T R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 493 DLNYVVQQLSQYLASPTDLHQQAATRVLRYVKGAPGQGLFFSATA---SLKLQAYSDSDW 549
D+ V S+++ P D H Q A R LRY+KG G+F SL Q +
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIFMLVITM*NSLDTQIVTRP-- 389
Query: 550 AGCPDTRRSINGYSIFLGSSLISW 573
GY+ LG ISW
Sbjct: 388 -----------GYAFHLGRGAISW 350
>AV769206
Length = 536
Score = 46.6 bits (109), Expect = 1e-05
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = -3
Query: 22 NRTKLDPRAQKCVFIGYRHGTKGFLLFDIKSREVLLSRHVLFHETEFPFKTM 73
N TKL +++CVFIGY KG+ D +S + +S+ VLFHE FP+ ++
Sbjct: 519 NSTKLSLHSKECVFIGYSINHKGYKCLD-QSGRIYISKDVLFHEHRFPYPSL 367
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 44.7 bits (104), Expect = 5e-05
Identities = 17/29 (58%), Positives = 23/29 (78%)
Frame = +2
Query: 620 LFCDNQSAIHIAQNPTFHERTKHIELDCH 648
L+CDNQ AI++ QN TFH R+KHI++ H
Sbjct: 173 LYCDNQGAIYLGQNSTFHSRSKHIDVRYH 259
Score = 33.5 bits (75), Expect = 0.12
Identities = 28/114 (24%), Positives = 51/114 (44%), Gaps = 5/114 (4%)
Frame = +3
Query: 571 ISWRTKKQTTVSRSSSEAEYRALAAIVCEVQWLSYLFQ--FLHLRVPFPVPLFC---DNQ 625
++W ++ Q V+ S++EAE+ A E+ W+ Q + H P+ C +
Sbjct: 27 VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSH-----PILCCIVITK 191
Query: 626 SAIHIAQNPTFHERTKHIELDCHVVRTKLQAGLIHLLPVSTRDQLADIFTKPLP 679
+ +A+ F + + +R L + L+ L + T D AD+ TK LP
Sbjct: 192 ALFTLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLP 353
>BP084259
Length = 385
Score = 43.9 bits (102), Expect = 9e-05
Identities = 19/58 (32%), Positives = 29/58 (49%)
Frame = -3
Query: 591 RALAAIVCEVQWLSYLFQFLHLRVPFPVPLFCDNQSAIHIAQNPTFHERTKHIELDCH 648
R + + E WL L + L +P +F +N +A ++ N FH KH+ LDCH
Sbjct: 179 RTIGSTAAEFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDCH 6
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,148,171
Number of Sequences: 28460
Number of extensions: 205906
Number of successful extensions: 1259
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 1236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1249
length of query: 694
length of database: 4,897,600
effective HSP length: 97
effective length of query: 597
effective length of database: 2,136,980
effective search space: 1275777060
effective search space used: 1275777060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0260.2