BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0253.6
(499 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP037118 186 8e-48
TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, parti... 85 3e-17
TC8572 similar to UP|Q9SCM3 (Q9SCM3) 40S ribosomal protein S2 ho... 42 2e-04
TC14095 homologue to UP|RS2_ARATH (P49688) 40S ribosomal protein... 42 2e-04
TC17927 UP|Q8GRY7 (Q8GRY7) bZIP with a Ring-finger motif, complete 32 0.18
AV428616 31 0.53
BP034429 30 0.91
TC15045 similar to AAR25638 (AAR25638) At1g55300, partial (66%) 30 1.2
AV422054 29 2.0
TC9118 similar to UP|Q84JE4 (Q84JE4) Squamosa promoter binding l... 29 2.0
CB828899 29 2.0
TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subun... 29 2.0
AV417236 28 2.7
TC11689 similar to GB|AAD34673.1|4966342|F3O9 ESTs gb|T04357 and... 28 2.7
BP046076 28 4.5
TC18821 weakly similar to UP|SRP_DROME (P52172) Box A-binding fa... 27 5.9
AV427157 27 7.7
CN825332 27 7.7
TC17592 similar to UP|OM20_SOLTU (P92792) Mitochondrial import r... 27 7.7
>BP037118
Length = 497
Score = 186 bits (472), Expect = 8e-48
Identities = 92/94 (97%), Positives = 92/94 (97%)
Frame = -2
Query: 406 ERHEEHTIAHAIQTSFKKTKVYLWPAPTATGMKAGRIVQTILHLAGFKNVKSKVVGSRNP 465
ERHEEHTIAHAIQTSFKKTKVYLWPAPTATGMKAGRIV TILHLAGFKNVKSKVVGSRNP
Sbjct: 496 ERHEEHTIAHAIQTSFKKTKVYLWPAPTATGMKAGRIVPTILHLAGFKNVKSKVVGSRNP 317
Query: 466 HNTVKAVFKALNAIETPRDVQEKFGRTVVEKYLL 499
HNTVKAVFKALNAIETPRDV EKFGRTVVEKYLL
Sbjct: 316 HNTVKAVFKALNAIETPRDVPEKFGRTVVEKYLL 215
>TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, partial (81%)
Length = 1294
Score = 84.7 bits (208), Expect = 3e-17
Identities = 50/152 (32%), Positives = 77/152 (49%)
Frame = +1
Query: 339 RKGFDVKLINVNRTCKVTKGGQVVKYTAMLACGNYNGVVGFAKAKGPAVPVALQKAYEKC 398
R GF+ +++ V R KV KGG+ +K+ A++ G+ G VG K V A+QK+
Sbjct: 541 RDGFEERVVQVRRITKVVKGGKQLKFRAVVVVGDKKGNVGVGVGKAKEVVAAVQKSAVNA 720
Query: 399 FQNLHFVERHEEHTIAHAIQTSFKKTKVYLWPAPTATGMKAGRIVQTILHLAGFKNVKSK 458
+N+ V + T H + KV L PA TG+ AG V+ +L +AG +N K
Sbjct: 721 RRNIVSVPMTKYLTFPHRSDGDYGAAKVMLRPASPGTGVIAGGAVRIVLEMAGVENALGK 900
Query: 459 VVGSRNPHNTVKAVFKALNAIETPRDVQEKFG 490
+GS N N +A A+ + R+V E+ G
Sbjct: 901 QLGSNNALNNARATVVAVQKMRQFREVAEERG 996
>TC8572 similar to UP|Q9SCM3 (Q9SCM3) 40S ribosomal protein S2 homolog
(At3g57490), partial (91%)
Length = 1076
Score = 42.4 bits (98), Expect = 2e-04
Identities = 35/131 (26%), Positives = 56/131 (42%), Gaps = 8/131 (6%)
Frame = +2
Query: 354 KVTKGGQVVKYTAMLACGNYNGVVGFAKAKGPAVPVALQKAYEKCFQNLHFVERH----- 408
K T+ GQ ++ A + G+ NG VG V A++ ++ V R
Sbjct: 368 KQTRAGQRTRFKAFVVVGDNNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNK 547
Query: 409 --EEHTIAHAIQTSFKKTKVYLWPAPTATGMKAGRIVQTILHLAGFKNVKSKVVGS-RNP 465
+ HT+ + V + PAP +G+ A R+ + +L AG +V + GS +
Sbjct: 548 IGKPHTVPCKVTGKCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTL 727
Query: 466 HNTVKAVFKAL 476
N VKA F L
Sbjct: 728 GNFVKATFDCL 760
>TC14095 homologue to UP|RS2_ARATH (P49688) 40S ribosomal protein S2,
partial (88%)
Length = 1134
Score = 42.4 bits (98), Expect = 2e-04
Identities = 35/131 (26%), Positives = 56/131 (42%), Gaps = 8/131 (6%)
Frame = +3
Query: 354 KVTKGGQVVKYTAMLACGNYNGVVGFAKAKGPAVPVALQKAYEKCFQNLHFVERH----- 408
K T+ GQ ++ A + G+ NG VG V A++ ++ V R
Sbjct: 348 KQTRAGQRTRFKAFVVVGDNNGHVGLGVKCSKEVATAIRGGIILAKLSVIPVRRGYWGNK 527
Query: 409 --EEHTIAHAIQTSFKKTKVYLWPAPTATGMKAGRIVQTILHLAGFKNVKSKVVGS-RNP 465
+ HT+ + V + PAP +G+ A R+ + +L AG +V + GS +
Sbjct: 528 IGKPHTVPCKVTGKCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTL 707
Query: 466 HNTVKAVFKAL 476
N VKA F L
Sbjct: 708 GNFVKATFDCL 740
>TC17927 UP|Q8GRY7 (Q8GRY7) bZIP with a Ring-finger motif, complete
Length = 1793
Score = 32.3 bits (72), Expect = 0.18
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +1
Query: 254 FEKEKQTIQGQGDAADDDDEQEFDDMKEK 282
+E K++ Q +GD DDDDE + DD++ +
Sbjct: 715 YESHKESSQMEGDDDDDDDEDDVDDLENE 801
>AV428616
Length = 356
Score = 30.8 bits (68), Expect = 0.53
Identities = 16/46 (34%), Positives = 25/46 (53%)
Frame = -3
Query: 315 LEEEIRDLAEERNELTEKKRRPLYRKGFDVKLINVNRTCKVTKGGQ 360
L+EE DL EE +EKK+R ++G +++ + V GGQ
Sbjct: 186 LKEEDLDLQEEEGW*SEKKKRTYTKQGIEMESVAVRTEVTTRHGGQ 49
>BP034429
Length = 567
Score = 30.0 bits (66), Expect = 0.91
Identities = 25/108 (23%), Positives = 48/108 (44%), Gaps = 4/108 (3%)
Frame = -1
Query: 255 EKEKQTIQGQGDAADDDDEQEFDDMKEKDNIILAKLDAIDKKLEEKLAELEYTFGKKGKV 314
E +T Q + +D + EFDD+ E + + + L + + E+++ + KKG
Sbjct: 531 EVANRTEQLRRMTGEDFEGLEFDDLLELEKTLPSGLKRVIELKEKRIMDEITAVQKKGIQ 352
Query: 315 LEEEIRDLAEERNELTEKKRRPLYRKGFDVKLI----NVNRTCKVTKG 358
LEEE + L ++ L + K L ++ + ++N C G
Sbjct: 351 LEEENKLLKQKMAMLCKGKSHFLLDSNITMQEVASSDSMNNVCSCNSG 208
>TC15045 similar to AAR25638 (AAR25638) At1g55300, partial (66%)
Length = 1050
Score = 29.6 bits (65), Expect = 1.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Frame = +1
Query: 62 DLLAQVEKDRLREKNERMRAGLDTADIDAEGEEDYMGVGPLIEKLEKEKLKECPELMRYE 121
+L+++VEKD L+ D + EG+E+ G + K L
Sbjct: 544 ELVSRVEKDLLKIMAGGTADSFDVVAAEQEGDENVRGANKKTAPTPEPKHDAPETLTNAG 723
Query: 122 EPTDSDSDEDE 132
+P SDSDE +
Sbjct: 724 DPDRSDSDESD 756
>AV422054
Length = 399
Score = 28.9 bits (63), Expect = 2.0
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1
Query: 3 GYLQYGRQTFRQILRCSTSAGN 24
GYLQYGR RQI+R S+ N
Sbjct: 139 GYLQYGRLGVRQIIRFRDSSSN 204
>TC9118 similar to UP|Q84JE4 (Q84JE4) Squamosa promoter binding
like-protein, partial (16%)
Length = 779
Score = 28.9 bits (63), Expect = 2.0
Identities = 17/62 (27%), Positives = 30/62 (47%)
Frame = +2
Query: 119 RYEEPTDSDSDEDEYEASQKKFDDFERKFKRHQDLLKNFTDADTLDDAFKWMNRIDKFED 178
R+EE + + +E+E E ++ D +R K+H+ + T D D K R +D
Sbjct: 11 RHEE--EEEEEEEEEEEKREDVRDHKRSRKKHRSHRSSHTSRDRDRDGRKHKRRHSSSKD 184
Query: 179 KH 180
K+
Sbjct: 185 KY 190
>CB828899
Length = 491
Score = 28.9 bits (63), Expect = 2.0
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +3
Query: 3 GYLQYGRQTFRQILRCSTSAGN 24
GYLQYGR RQI+R S+ N
Sbjct: 156 GYLQYGRLGVRQIIRFRDSSSN 221
>TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subunit-like
protein, partial (20%)
Length = 979
Score = 28.9 bits (63), Expect = 2.0
Identities = 15/58 (25%), Positives = 33/58 (56%)
Frame = -1
Query: 241 QLGPTSDALQNAGFEKEKQTIQGQGDAADDDDEQEFDDMKEKDNIILAKLDAIDKKLE 298
++G SDA+ GFE + I+ + + ++++E+E + E + I +++ D +LE
Sbjct: 190 EMGIESDAVMRLGFEGR*ERIEIRDEEEEEEEEEEEEGFVEIEIEIEIEIEI*DLRLE 17
>AV417236
Length = 399
Score = 28.5 bits (62), Expect = 2.7
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -1
Query: 305 EYTFGKKGKVLEEEIRDLAEERNELTEKKR 334
E + KKGKVLE E+ + AEE EK+R
Sbjct: 141 ELKWEKKGKVLEGEMEEGAEEEKVNREKQR 52
>TC11689 similar to GB|AAD34673.1|4966342|F3O9 ESTs gb|T04357 and
gb|AA595092 come from this gene. {Arabidopsis thaliana;}
, partial (26%)
Length = 573
Score = 28.5 bits (62), Expect = 2.7
Identities = 14/36 (38%), Positives = 19/36 (51%)
Frame = +3
Query: 457 SKVVGSRNPHNTVKAVFKALNAIETPRDVQEKFGRT 492
S + R+P +KA FKA E P+ +EK G T
Sbjct: 54 SNLAPDRHPERRLKAAFKAFEEAELPKLKEEKPGLT 161
>BP046076
Length = 593
Score = 27.7 bits (60), Expect = 4.5
Identities = 15/53 (28%), Positives = 21/53 (39%)
Frame = -2
Query: 136 SQKKFDDFERKFKRHQDLLKNFTDADTLDDAFKWMNRIDKFEDKHFRLRPEYR 188
S+K RK + + + +D D LDD +KW K R YR
Sbjct: 541 SEKSKVKVRRKLREPRFCFQTRSDVDVLDDGYKWRKYGQKVVKNSLHPRSYYR 383
>TC18821 weakly similar to UP|SRP_DROME (P52172) Box A-binding factor (ABF)
(Serpent protein) (GATA-binding factor-B) (Transcription
factor GATA-B) (dGATA-B), partial (3%)
Length = 767
Score = 27.3 bits (59), Expect = 5.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +2
Query: 18 CSTSAGNVVRNSVRSQLGCEGTALSSSYSGK 48
C+ S+G RNS S G A + S+SG+
Sbjct: 218 CARSSGGCARNSAGSARSSAGLATAKSFSGR 310
>AV427157
Length = 379
Score = 26.9 bits (58), Expect = 7.7
Identities = 11/50 (22%), Positives = 26/50 (52%), Gaps = 7/50 (14%)
Frame = +3
Query: 127 DSDEDEYEASQKKFDDFE-------RKFKRHQDLLKNFTDADTLDDAFKW 169
D + DE E+ ++K + + R + + +++ ++ D LDD ++W
Sbjct: 75 DGEGDESESKRRKLESYATELSGATRAIREPRVVVQTTSEVDILDDGYRW 224
>CN825332
Length = 612
Score = 26.9 bits (58), Expect = 7.7
Identities = 15/36 (41%), Positives = 22/36 (60%), Gaps = 1/36 (2%)
Frame = +3
Query: 24 NVVRNSVRSQLGCEGTALS-SSYSGKSLYSTSSDAR 58
+VV +S+RSQLGC T S S Y+ K + S+ +
Sbjct: 87 HVVCSSIRSQLGCSKTLASRSHYAAKFPVTLKSEGK 194
>TC17592 similar to UP|OM20_SOLTU (P92792) Mitochondrial import receptor
subunit TOM20 (Translocase of outer membrane 20 kDa
subunit), partial (58%)
Length = 482
Score = 26.9 bits (58), Expect = 7.7
Identities = 19/56 (33%), Positives = 27/56 (47%), Gaps = 4/56 (7%)
Frame = +1
Query: 112 KECPELMRYEEPTDSDSDEDEYEASQKKFDD---FERKFKRHQ-DLLKNFTDADTL 163
K+ P+ R D+D+D D + Q +FD FE K + + KN DAD L
Sbjct: 49 KKNPKFSRSLSKGDADADADAMDMQQSEFDRLLFFEHARKAAEAEYAKNPLDADNL 216
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.134 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,566,468
Number of Sequences: 28460
Number of extensions: 73808
Number of successful extensions: 485
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 476
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 485
length of query: 499
length of database: 4,897,600
effective HSP length: 94
effective length of query: 405
effective length of database: 2,222,360
effective search space: 900055800
effective search space used: 900055800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0253.6