BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0253.3
(594 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10521 172 2e-43
AV428867 29 1.9
BP049555 29 1.9
TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromos... 29 2.5
TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin at... 29 2.5
TC8736 similar to PIR|T06396|T06396 isoprenylated protein - soyb... 29 2.5
TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetyla... 28 4.2
AW719523 28 5.5
AV418628 28 5.5
TC9445 similar to PIR|T08956|T08956 AIM1 protein - Arabidopsis t... 28 5.5
TC14310 homologue to UP|Q9M3H6 (Q9M3H6) Histone H2B, partial (92%) 28 5.5
TC20256 similar to UP|CYB_LAMGT (Q94T68) Cytochrome b, partial (6%) 27 7.2
AV408295 27 7.2
BP034655 27 7.2
TC17570 similar to UP|O48933 (O48933) Histidinol dehydrogenase (... 27 7.2
TC10919 similar to UP|SRS1_ORYSA (P83649) Salt-stress root prote... 27 9.4
>TC10521
Length = 751
Score = 172 bits (435), Expect = 2e-43
Identities = 83/84 (98%), Positives = 83/84 (98%)
Frame = +2
Query: 511 EVHKLLGTVLVDKDAALRWKVRCAEIFPYSVYFEGRHSSASPNSALNQICKKSFENGSSS 570
EVHKLLGTVLVDKDA LRWKVRCAEIFPYSVYFEGRHSSASPNSALNQICKKSFENGSSS
Sbjct: 2 EVHKLLGTVLVDKDAXLRWKVRCAEIFPYSVYFEGRHSSASPNSALNQICKKSFENGSSS 181
Query: 571 HSVGNCKVESITSNGKLDTFKDLT 594
HSVGNCKVESITSNGKLDTFKDLT
Sbjct: 182 HSVGNCKVESITSNGKLDTFKDLT 253
>AV428867
Length = 628
Score = 29.3 bits (64), Expect = 1.9
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 251 LHSHVYFHKAAVGAIRCYIKLHDSPPKSTA 280
L +FHK +G +C++K+ PPK TA
Sbjct: 511 LRKSFFFHKGWLGVFKCFLKIW--PPKQTA 594
>BP049555
Length = 555
Score = 29.3 bits (64), Expect = 1.9
Identities = 14/57 (24%), Positives = 32/57 (55%)
Frame = +2
Query: 287 SKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAKPVDPDPRGEK 343
S L ++ +Q+Q +AEA+ +++ EEK EE + + K++ + + + + E+
Sbjct: 206 STFLEQMGEEAKQEQPQAEAKPEEKTEEKAEEKAEEKPAAEEKKEEEKPEEEKKEEE 376
>TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromosome 3, P1
clone: MSJ11, partial (39%)
Length = 1403
Score = 28.9 bits (63), Expect = 2.5
Identities = 12/36 (33%), Positives = 22/36 (60%)
Frame = +1
Query: 274 SPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAK 309
+P ++ A++ + K+LP +K+K RQK K + K
Sbjct: 376 TPQRNVAKDHQLLKKMLPGKKRKRRQKMWKCKKLVK 483
>TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment
subunit precursor, partial (3%)
Length = 1235
Score = 28.9 bits (63), Expect = 2.5
Identities = 21/84 (25%), Positives = 37/84 (44%), Gaps = 8/84 (9%)
Frame = +1
Query: 273 DSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKE--------AEEKNEESSASGI 324
++P EEDD+++ L KKK + ++ AE E E++ E + G
Sbjct: 727 EAPQAEEGEEDDEINDLFKMGKKKKKNERSPAEIALLVENVMAELEVTAEEDAELNRQGK 906
Query: 325 SKSGKRQAKPVDPDPRGEKLLQVE 348
K + P+ + +K LQ+E
Sbjct: 907 PAINKLKKLPLLTEVLSKKQLQLE 978
>TC8736 similar to PIR|T06396|T06396 isoprenylated protein - soybean
(fragment) {Glycine max;}, partial (84%)
Length = 1268
Score = 28.9 bits (63), Expect = 2.5
Identities = 18/69 (26%), Positives = 29/69 (41%)
Frame = +2
Query: 275 PPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQAKP 334
PP+ +++ D K +KKK +K + A KK+ EK + + K + K
Sbjct: 473 PPEPEKKKEADKPKAAEHEKKKDGEKPKAAAEPEKKKDSEKPKAAEPEKKKDGDKEKPKS 652
Query: 335 VDPDPRGEK 343
P EK
Sbjct: 653 DGPKKGDEK 679
>TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (65%)
Length = 1341
Score = 28.1 bits (61), Expect = 4.2
Identities = 17/68 (25%), Positives = 29/68 (42%)
Frame = +1
Query: 273 DSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSASGISKSGKRQA 332
DS +S E DD+ P+ KK+ Q +++ A K + +A+ GK+
Sbjct: 616 DSDDESDDESDDEEET--PTPAKKVDQGKKRPNESASKTPVSGKKAKNATPEKPDGKKNV 789
Query: 333 KPVDPDPR 340
P P+
Sbjct: 790 HTATPHPK 813
>AW719523
Length = 543
Score = 27.7 bits (60), Expect = 5.5
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +2
Query: 294 KKKLRQKQRKAEARAKKEAEEK 315
+ K R+KQRK + KKEA+EK
Sbjct: 440 RDKSREKQRKKNLQVKKEAKEK 505
>AV418628
Length = 309
Score = 27.7 bits (60), Expect = 5.5
Identities = 22/68 (32%), Positives = 32/68 (46%), Gaps = 12/68 (17%)
Frame = -1
Query: 29 PSLFSDLSSLY----DHPGKANILEQLILELENSIRTTGTYPGRVEKEP--------PST 76
P L S+LSS + PG++ L L+ EL + T PGR P +
Sbjct: 309 PLLLSELSSFFAPVRTTPGRSLSLPLLLSELSSFFAPVRTTPGRSLSLPLLLLSELLSES 130
Query: 77 LMWILFLL 84
L+++LFLL
Sbjct: 129 LLFLLFLL 106
>TC9445 similar to PIR|T08956|T08956 AIM1 protein - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (58%)
Length = 1401
Score = 27.7 bits (60), Expect = 5.5
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -2
Query: 536 IFPYSVYFEGRHSSASPNSALNQICKKSFENGSSS 570
+FP S F+ + SP A+ + C+K F N S+
Sbjct: 908 VFPISPTFDQKLKQCSPKCAIGKGCRKFFSNCHSN 804
>TC14310 homologue to UP|Q9M3H6 (Q9M3H6) Histone H2B, partial (92%)
Length = 502
Score = 27.7 bits (60), Expect = 5.5
Identities = 16/54 (29%), Positives = 30/54 (54%), Gaps = 2/54 (3%)
Frame = +3
Query: 271 LHDSPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKE--AEEKNEESSAS 322
L PP + A+ + ++ P+ +K +K+ KAE + KE +E+K ++S S
Sbjct: 39 LSSPPPMAPAKAEKKPAEKKPAAEKAPAEKKPKAEKKIAKEGGSEKKKKKSKKS 200
>TC20256 similar to UP|CYB_LAMGT (Q94T68) Cytochrome b, partial (6%)
Length = 543
Score = 27.3 bits (59), Expect = 7.2
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -1
Query: 278 STAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNEESSA 321
S ++EDD K K ++K++KAE+ AKK ++ E+ A
Sbjct: 543 SDSKEDDKTDK-----NNKKKKKKKKAESNAKKPKSDRFEKLEA 427
>AV408295
Length = 426
Score = 27.3 bits (59), Expect = 7.2
Identities = 21/84 (25%), Positives = 42/84 (50%), Gaps = 7/84 (8%)
Frame = +3
Query: 259 KAAVGAIRCYIKLHDSPPKSTAEE----DDDMSKLLPSQKKKLRQKQRKAEAR--AKKEA 312
K V A K+ + K T EE +++ + + ++KK ++++KAEA A
Sbjct: 153 KDEVAAAATEEKVEEEEEKKTEEEKKPAEEEKKEEVVEEEKKPAEEEKKAEAEGEATTSG 332
Query: 313 EEKN-EESSASGISKSGKRQAKPV 335
EEKN E+ + ++G++ + +
Sbjct: 333 EEKNVVEAVTEVVVEAGEKTVEAI 404
>BP034655
Length = 517
Score = 27.3 bits (59), Expect = 7.2
Identities = 12/44 (27%), Positives = 26/44 (58%)
Frame = +2
Query: 274 SPPKSTAEEDDDMSKLLPSQKKKLRQKQRKAEARAKKEAEEKNE 317
SPP + + D + +KKK ++K++K + + KK+ ++K +
Sbjct: 95 SPPHISPPQILDSPTTMKRKKKKKKKKKKKKKKKKKKKKKKKTK 226
>TC17570 similar to UP|O48933 (O48933) Histidinol dehydrogenase (Fragment),
partial (51%)
Length = 811
Score = 27.3 bits (59), Expect = 7.2
Identities = 8/24 (33%), Positives = 16/24 (66%)
Frame = -3
Query: 212 LAVEKHYTDITEDQFDFHSYCLRK 235
L+ +H++ +T +Q + H YC R+
Sbjct: 434 LSCSQHFSQLTSEQHERHKYCCRR 363
>TC10919 similar to UP|SRS1_ORYSA (P83649) Salt-stress root protein RS1,
partial (14%)
Length = 551
Score = 26.9 bits (58), Expect = 9.4
Identities = 17/62 (27%), Positives = 30/62 (47%), Gaps = 6/62 (9%)
Frame = +3
Query: 288 KLLPSQKKKLRQKQRKAEARAKK------EAEEKNEESSASGISKSGKRQAKPVDPDPRG 341
+++ ++KK +K + AE +K + EEK E + + K + KPV+P
Sbjct: 42 EIVVEEEKKEEEKPQVAEETTEKVESEPPKEEEKPAEPVVAEAPPAAKEEEKPVEPVAEV 221
Query: 342 EK 343
EK
Sbjct: 222 EK 227
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,594,133
Number of Sequences: 28460
Number of extensions: 126496
Number of successful extensions: 710
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 701
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 707
length of query: 594
length of database: 4,897,600
effective HSP length: 95
effective length of query: 499
effective length of database: 2,193,900
effective search space: 1094756100
effective search space used: 1094756100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0253.3