BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0252b.2
(515 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP051903 101 3e-22
BP078889 100 7e-22
BP055246 94 7e-20
TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related prote... 84 4e-17
BG662008 77 9e-15
AI967582 70 6e-13
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 48 4e-06
BP075190 47 6e-06
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 46 1e-05
TC11829 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, p... 45 2e-05
TC15623 similar to UP|P93486 (P93486) Glycine-rich RNA-binding p... 45 4e-05
TC8818 weakly similar to PIR|T48456|T48456 rna binding protein-l... 45 4e-05
BP074232 44 6e-05
TC14147 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprote... 44 8e-05
TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1... 42 2e-04
TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 42 2e-04
AV409413 42 3e-04
TC14208 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 41 5e-04
TC10587 weakly similar to GB|AAC50895.1|1518802|HSU63289 CUG-BP/... 40 7e-04
TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotei... 40 0.001
>BP051903
Length = 342
Score = 101 bits (251), Expect = 3e-22
Identities = 50/77 (64%), Positives = 63/77 (80%)
Frame = +2
Query: 48 VDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDG 107
VDGI + Y+QIRGLFAQFGR+VNVFVQR++K+GR FRFGFVRF S +VA+ AI L+G
Sbjct: 68 VDGIPDRTDYYQIRGLFAQFGRVVNVFVQRQRKIGRLFRFGFVRFASLEVASKAIDFLNG 247
Query: 108 VRLGGASLSVGLAKPPQ 124
RLG A+LSV +A+ P+
Sbjct: 248 FRLGDAALSVSMARFPR 298
>BP078889
Length = 474
Score = 100 bits (248), Expect = 7e-22
Identities = 57/118 (48%), Positives = 72/118 (60%), Gaps = 6/118 (5%)
Frame = +1
Query: 10 GSAVGDGVDVGDEAADEEDGSTPN------QKCSGVSRVCVSLFVDGIAESVSYHQIRGL 63
G+ G D+G E + G T N + G R + FVDGI E++ YH +RGL
Sbjct: 118 GALGGPIRDLGGEEVASDKGETRNDAGGLNRNNGGERRRKPTPFVDGIEEAIEYHHLRGL 297
Query: 64 FAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDGVRLGGASLSVGLAK 121
FA FGR+ N+FVQRK K GRRFRFGFVRF S + AA LDG+R+GG +LSV A+
Sbjct: 298 FALFGRISNLFVQRKHKFGRRFRFGFVRFFSEEQAAAVAFVLDGLRVGGTALSVAPAR 471
>BP055246
Length = 544
Score = 93.6 bits (231), Expect = 7e-20
Identities = 47/82 (57%), Positives = 55/82 (66%)
Frame = -3
Query: 434 RGRKPRQEPPEGCVGLVVVDGGGPSSSAILPSVCSTPLVSESTHRGRYWTRATKAWMLGK 493
R R P Q P VG V VD GG S+SAI+PS + PL S S RYW RATKAW++GK
Sbjct: 542 RDR*PHQVPLVTGVGSVAVDVGGSSTSAIMPSFRTAPLDSGSAPSSRYWMRATKAWLIGK 363
Query: 494 LVGCSFDGGDEAAIRGLAEEME 515
LVGC+FDGGDE GL +E+E
Sbjct: 362 LVGCTFDGGDEETTHGLTQEIE 297
>TC18897 weakly similar to UP|Q9XEV3 (Q9XEV3) TIA-1 related protein, partial
(5%)
Length = 481
Score = 84.3 bits (207), Expect = 4e-17
Identities = 41/96 (42%), Positives = 64/96 (65%)
Frame = +1
Query: 16 GVDVGDEAADEEDGSTPNQKCSGVSRVCVSLFVDGIAESVSYHQIRGLFAQFGRLVNVFV 75
GVD ++ ++E G P G +LF+DGI+E +Y+ IRGLF Q G L+NVF+
Sbjct: 199 GVDSDEDLLEDEAGEEP--AIGGGRAPSFTLFIDGISEPTNYYHIRGLFGQIGSLLNVFL 372
Query: 76 QRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDGVRLG 111
Q+++++ R FRFGFVR+++ VA++AI +GV +G
Sbjct: 373 QKQRRIRRNFRFGFVRYSTRDVASMAINLFNGVLMG 480
>BG662008
Length = 457
Score = 76.6 bits (187), Expect = 9e-15
Identities = 35/60 (58%), Positives = 49/60 (81%)
Frame = +3
Query: 44 VSLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIK 103
V+LFVDGI++ V Y QIRGLF+ G L+NVFVQR++++GR FRFGFVRF S ++A+ ++
Sbjct: 99 VTLFVDGISDFVDYSQIRGLFSHIGSLLNVFVQRQRRIGRAFRFGFVRFPSVELASSMVR 278
>AI967582
Length = 356
Score = 70.5 bits (171), Expect = 6e-13
Identities = 52/116 (44%), Positives = 65/116 (55%), Gaps = 7/116 (6%)
Frame = +2
Query: 73 VFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDGVRLGGASLSVGLAKPPQKVVSRSDA 132
VFVQRK ++GRRF FGFV F SW A A+ L+G+++GGA LSV ++ P SRS A
Sbjct: 20 VFVQRKHEVGRRFHFGFVHFLSWSDAXTAVARLNGMKIGGAHLSVTVSTFP----SRSGA 187
Query: 133 KVANV---CSVIKKVD--GSSGL--TGKRKESVSGPFISSWRDVVVGNTKGSRLVS 181
N + K D GS + + K SV + SWRDVVVG G RL S
Sbjct: 188 PSGNSGFRALGLSKSD*RGSISVSQSSMLKGSVFPSTLPSWRDVVVGKL-GVRLPS 352
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 47.8 bits (112), Expect = 4e-06
Identities = 26/85 (30%), Positives = 46/85 (53%)
Frame = +1
Query: 37 SGVSRVCVSLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWK 96
S ++ V FV G+A + H + F+ +G +++ V ++ GR FGFV FTS +
Sbjct: 61 SAMADVEYRCFVGGLAWTTDSHTLEQAFSSYGEIIDSKVVNDRETGRSRGFGFVTFTSEE 240
Query: 97 VAAVAIKSLDGVRLGGASLSVGLAK 121
AI+ ++G L G +++V A+
Sbjct: 241 AMRSAIEGMNGNELDGRNITVNEAQ 315
>BP075190
Length = 512
Score = 47.4 bits (111), Expect = 6e-06
Identities = 26/63 (41%), Positives = 37/63 (58%), Gaps = 3/63 (4%)
Frame = -1
Query: 454 GGGPSSSAILPSVCSTPLVSESTHRG---RYWTRATKAWMLGKLVGCSFDGGDEAAIRGL 510
GGG + + S+ L S +H G Y+TR+ +AW+LGK++G + G D AIRGL
Sbjct: 440 GGG--NGFLYASISIEMLESGPSHAGSVGEYFTRSKRAWLLGKVIGLEYPGSDLEAIRGL 267
Query: 511 AEE 513
EE
Sbjct: 266 EEE 258
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 46.2 bits (108), Expect = 1e-05
Identities = 31/88 (35%), Positives = 44/88 (49%)
Frame = +2
Query: 44 VSLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIK 103
VSLF A S S +++ F +FG+L V K GR FGFV F K AI
Sbjct: 56 VSLFYWWPAWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAID 235
Query: 104 SLDGVRLGGASLSVGLAKPPQKVVSRSD 131
+++G+ L G +++V A+P R D
Sbjct: 236 AMNGMDLDGRTITVDKAQPQGSSRDRDD 319
>TC11829 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, partial (4%)
Length = 738
Score = 45.4 bits (106), Expect = 2e-05
Identities = 33/112 (29%), Positives = 57/112 (50%), Gaps = 8/112 (7%)
Frame = +1
Query: 24 ADEEDGSTPNQKCSGVSRVCV-------SLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQ 76
A+ GST +G+ V +L++ + ++ + LF QFG +V V
Sbjct: 136 ANNNGGSTGGAAQAGLGAGAVKKEIDDTNLYIGYLPPTLEDDGLIQLFQQFGEIVMAKVI 315
Query: 77 RKKKLGRRFRFGFVRFTSWKVAAVAIKSLDGVRLGGASLSVGLA-KPPQKVV 127
+ + G +GFV++ +A AI +++G RL G +++V +A KPPQ VV
Sbjct: 316 KDRMTGLSKGYGFVKYADITMANNAILAMNGYRLEGRTIAVRVAGKPPQPVV 471
>TC15623 similar to UP|P93486 (P93486) Glycine-rich RNA-binding protein
PsGRBP, partial (88%)
Length = 683
Score = 44.7 bits (104), Expect = 4e-05
Identities = 25/75 (33%), Positives = 38/75 (50%)
Frame = +1
Query: 46 LFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSL 105
LF+ G++ V + F+ FG + V + GR FGFV F S + A+ A+ S+
Sbjct: 190 LFIGGLSYGVDDKSLEDAFSSFGTVAEARVIVDRDTGRSRGFGFVSFDSEESASSALSSM 369
Query: 106 DGVRLGGASLSVGLA 120
DG L G ++ V A
Sbjct: 370 DGQDLNGRNIRVSYA 414
>TC8818 weakly similar to PIR|T48456|T48456 rna binding protein-like -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(90%)
Length = 1079
Score = 44.7 bits (104), Expect = 4e-05
Identities = 25/79 (31%), Positives = 37/79 (46%)
Frame = +3
Query: 46 LFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSL 105
L+V I +I G F QFG + + + R KK G+ FGF+ F S +VA + ++
Sbjct: 219 LYVGRIPHGFYEKEIEGYFGQFGTIKRLRIARNKKTGKSRHFGFIEFESAEVAKIVADTM 398
Query: 106 DGVRLGGASLSVGLAKPPQ 124
L L V + P Q
Sbjct: 399 HNYLLFAHLLQVHVMPPEQ 455
>BP074232
Length = 344
Score = 43.9 bits (102), Expect = 6e-05
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = -1
Query: 481 YWTRATKAWMLGKLVGCSFDGGDEAAIRGLAEEME 515
Y+TRA K W+LGK G F G + AIRGLAE ++
Sbjct: 341 YFTRAKKVWILGKYAGLEFPGSQDEAIRGLAEFLQ 237
>TC14147 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprotein A,
chloroplast precursor (CP29A), partial (63%)
Length = 1602
Score = 43.5 bits (101), Expect = 8e-05
Identities = 28/78 (35%), Positives = 39/78 (49%)
Frame = +3
Query: 48 VDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDG 107
V +A V + LF + GR+V+ V ++ GR FGFV F+S AI+SLDG
Sbjct: 750 VGNLAWGVDNAALESLFREQGRVVDAKVIYDRESGRSRGFGFVTFSSPDEVNSAIRSLDG 929
Query: 108 VRLGGASLSVGLAKPPQK 125
L G ++ V A K
Sbjct: 930 ADLNGRAIKVSQADSKPK 983
>TC8296 similar to GB|AAN28790.1|23505861|AY143851 At5g64200/MSJ1_4
{Arabidopsis thaliana;}, partial (55%)
Length = 1276
Score = 42.4 bits (98), Expect = 2e-04
Identities = 23/77 (29%), Positives = 41/77 (52%)
Frame = +2
Query: 45 SLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKS 104
SL V I + + LF ++G++V++F+ + ++ G F FVR+ A+ A+
Sbjct: 134 SLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAVDR 313
Query: 105 LDGVRLGGASLSVGLAK 121
LDG + G ++V AK
Sbjct: 314 LDGRMVDGREITVQFAK 364
>TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (77%)
Length = 1576
Score = 42.4 bits (98), Expect = 2e-04
Identities = 25/87 (28%), Positives = 46/87 (52%)
Frame = +3
Query: 45 SLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKS 104
++FV + +V+ +R +F +G LV+V + + K R GFV+F A A++
Sbjct: 945 TIFVGNLDPNVTDDHLRQVFGLYGDLVHVKIPQGK------RCGFVQFADRSCAEEALRV 1106
Query: 105 LDGVRLGGASLSVGLAKPPQKVVSRSD 131
L+G LGG ++ + + P ++SD
Sbjct: 1107 LNGTLLGGQNVRLSWGRSPSNKQTQSD 1187
Score = 27.3 bits (59), Expect = 6.1
Identities = 20/64 (31%), Positives = 28/64 (43%), Gaps = 9/64 (14%)
Frame = +3
Query: 64 FAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDGV---------RLGGAS 114
FA G L V V R K + +GF+ FTS A +++ +G RL A+
Sbjct: 393 FAHTGELATVKVIRNKNTNQSEGYGFLEFTSRAGAERILQTYNGTIMPNGGQNFRLNWAT 572
Query: 115 LSVG 118
S G
Sbjct: 573 FSAG 584
>AV409413
Length = 422
Score = 41.6 bits (96), Expect = 3e-04
Identities = 30/115 (26%), Positives = 51/115 (44%)
Frame = +2
Query: 48 VDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKSLDG 107
V GI SVS +++G F+++G++V + R R FGF+ F + KV + +
Sbjct: 2 VGGIPTSVSEDELKGFFSKYGKVVEHEIIRDHTTKRSRGFGFIVFDNEKVVDTILTDGNM 181
Query: 108 VRLGGASLSVGLAKPPQKVVSRSDAKVANVCSVIKKVDGSSGLTGKRKESVSGPF 162
+ + G + + A+P + S S A+ DG SG SG +
Sbjct: 182 IDMAGTQVEIKKAEPKKSSNSGSLPPFASDSRPRSYNDGFSGFADSYGSFGSGGY 346
>TC14208 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (41%)
Length = 837
Score = 40.8 bits (94), Expect = 5e-04
Identities = 24/88 (27%), Positives = 46/88 (52%)
Frame = +2
Query: 45 SLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKS 104
++FV + +V+ +R +F+ +G LV+V + K R GFV+F A A++
Sbjct: 194 TIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGK------RCGFVQFADRSGAEEALRV 355
Query: 105 LDGVRLGGASLSVGLAKPPQKVVSRSDA 132
L+G LGG ++ + + P ++ D+
Sbjct: 356 LNGTLLGGQNVRLSWGRSPSNKQAQPDS 439
>TC10587 weakly similar to GB|AAC50895.1|1518802|HSU63289 CUG-BP/hNab50
{Homo sapiens;} , partial (19%)
Length = 482
Score = 40.4 bits (93), Expect = 7e-04
Identities = 24/73 (32%), Positives = 36/73 (48%)
Frame = +1
Query: 45 SLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIKS 104
+LF+ I + + F FGR+++ V K G FGFV + S + A AI
Sbjct: 16 NLFIYHIPQEFGDQDLANAFQPFGRVISAKVFVDKATGVSKCFGFVSYDSPEAAQSAISM 195
Query: 105 LDGVRLGGASLSV 117
++G +LGG L V
Sbjct: 196 MNGYQLGGKKLKV 234
>TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotein,
chloroplast precursor, partial (28%)
Length = 717
Score = 39.7 bits (91), Expect = 0.001
Identities = 23/78 (29%), Positives = 41/78 (52%)
Frame = +3
Query: 44 VSLFVDGIAESVSYHQIRGLFAQFGRLVNVFVQRKKKLGRRFRFGFVRFTSWKVAAVAIK 103
+S++V + SV ++ +F + G + N + ++ GR FGFV +S AI
Sbjct: 78 LSVYVGNLPWSVDTARLEEIFREHGNVENARIVMDRETGRSRGFGFVTMSSEADINGAIA 257
Query: 104 SLDGVRLGGASLSVGLAK 121
+LDG L G ++ V +A+
Sbjct: 258 ALDGQSLDGRTIRVSVAE 311
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.139 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,495,030
Number of Sequences: 28460
Number of extensions: 147739
Number of successful extensions: 818
Number of sequences better than 10.0: 71
Number of HSP's better than 10.0 without gapping: 805
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 815
length of query: 515
length of database: 4,897,600
effective HSP length: 94
effective length of query: 421
effective length of database: 2,222,360
effective search space: 935613560
effective search space used: 935613560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0252b.2