BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0249.15
(521 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {... 70 8e-13
TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase... 65 2e-11
TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J... 52 3e-07
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 49 3e-06
TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%) 46 2e-05
TC16655 homologue to UP|O99018 (O99018) Chloroplast protease pre... 45 2e-05
TC9659 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATP... 45 2e-05
AV422700 45 4e-05
CB826675 39 0.002
TC9051 UP|PRS7_ARATH (Q9SSB5) 26S protease regulatory subunit 7 ... 39 0.003
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas... 38 0.005
AV408284 38 0.005
TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), pa... 37 0.006
TC12787 weakly similar to UP|MSP1_YEAST (P28737) MSP1 protein (T... 37 0.008
TC12379 homologue to UP|O04327 (O04327) Cell division protein Ft... 35 0.039
TC14829 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 34 0.051
CN825587 34 0.067
AW719510 33 0.087
AV426828 33 0.087
TC11760 homologue to UP|PRS4_ORYSA (P46466) 26S protease regulat... 33 0.15
>TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
thaliana;}, partial (87%)
Length = 1385
Score = 70.1 bits (170), Expect = 8e-13
Identities = 54/180 (30%), Positives = 89/180 (49%), Gaps = 8/180 (4%)
Frame = +3
Query: 217 STFETLAMEPEAKKAVMEDLNRFVKRKEFYKKVGRAWKRGYLLYGPPGTGKSSLIAAMAN 276
ST++ + + K + E + +K E ++ +G A +G LLYGPPGTGK+ L A+A+
Sbjct: 318 STYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAH 497
Query: 277 -----YLKFDIFDLQLGNIVRDSDLRKLLLATANR---SILVIEDIDCSVDLTERRNGGD 328
+++ +L I S + + L A SI+ +++ID G
Sbjct: 498 HTDCTFIRVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGX 677
Query: 329 GQTDVQAHRESDGWMQLTLSGLLNFIDGLWSSCGDERIIVFTTNHKERLDPALLRPGRMD 388
G ++VQ T+ LLN +DG +S ++ ++ TN + LD ALLRPGR+D
Sbjct: 678 GDSEVQR----------TMLELLNQLDGFEAS--NKIKVLMATNRIDILDQALLRPGRID 821
>TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase subunit
RPT2a, partial (69%)
Length = 916
Score = 65.5 bits (158), Expect = 2e-11
Identities = 50/147 (34%), Positives = 75/147 (51%), Gaps = 2/147 (1%)
Frame = +1
Query: 244 EFYKKVGRAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIFDLQLGNIVRD--SDLRKLLL 301
E Y+ +G +G +LYG PGTGK+ L A+AN + +++ D KL+
Sbjct: 454 ELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLV- 630
Query: 302 ATANRSILVIEDIDCSVDLTERRNGGDGQTDVQAHRESDGWMQLTLSGLLNFIDGLWSSC 361
V +D+ S+ + + G AH + +Q T+ LLN +DG + S
Sbjct: 631 ---RELFRVADDLSPSIVFIDEID-AVGTKRYDAHSGGEREIQRTMLELLNQLDG-FDSR 795
Query: 362 GDERIIVFTTNHKERLDPALLRPGRMD 388
GD ++I+ TN E LDPALLRPGR+D
Sbjct: 796 GDVKVIL-ATNRIESLDPALLRPGRID 873
>TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J8.110 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 771
Score = 51.6 bits (122), Expect = 3e-07
Identities = 43/174 (24%), Positives = 82/174 (46%), Gaps = 8/174 (4%)
Frame = +3
Query: 218 TFETLAMEPEAKKAVMEDLNRFVKRKEFYKKVGRAWKRGYLLYGPPGTGKSSLIAAMANY 277
TF + E K+++ E + ++R + +K RG LL+GPPGTGK+ L A+AN
Sbjct: 195 TFGDIGAMDEIKESLQELVMLPLRRPDLFKGGLLKPCRGILLFGPPGTGKTMLAXAIANE 374
Query: 278 LKFDIFDLQLGNIVR------DSDLRKL--LLATANRSILVIEDIDCSVDLTERRNGGDG 329
++ + I + ++R L L A +I+ ++++D L +R G+
Sbjct: 375 AGASFINVSMSTITSKWFGEDEKNVRALFSLAAKVAPTIIFVDEVDSM--LGQRTRVGEH 548
Query: 330 QTDVQAHRESDGWMQLTLSGLLNFIDGLWSSCGDERIIVFTTNHKERLDPALLR 383
+ M+ + + DGL ++ G++ +++ TN LD A++R
Sbjct: 549 EA-----------MRKIKNEFMTHWDGLLTAPGEQILVLAATNRPFDLDEAIIR 677
>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (65%)
Length = 901
Score = 48.5 bits (114), Expect = 3e-06
Identities = 38/162 (23%), Positives = 68/162 (41%), Gaps = 35/162 (21%)
Frame = +3
Query: 151 LRSEKRYFELSFLKEHKEMVLECYLPFILDRAKEMKDEDRVLKIHTLNASYCYNVIKWDS 210
++ E++ + L+ +E+ +P ++ + EM D++ + T ++Y ++ +
Sbjct: 186 VKDEQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMADQNNGIVGSTTGSNYYVRILSTIN 365
Query: 211 INLEHPSTFETL---------AMEPEA--------------------------KKAVMED 235
L PS L + PEA K+ + E
Sbjct: 366 RELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREA 545
Query: 236 LNRFVKRKEFYKKVGRAWKRGYLLYGPPGTGKSSLIAAMANY 277
+ + E YK++G RG LLYGPPGTGK+ L A+AN+
Sbjct: 546 VELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANH 671
>TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%)
Length = 532
Score = 45.8 bits (107), Expect = 2e-05
Identities = 42/140 (30%), Positives = 66/140 (47%), Gaps = 9/140 (6%)
Frame = +1
Query: 253 WKRGYLLYGPPGTGKSSLIAAMANYLKFDIFDLQLGNIVR----DSD-LRKLLLATANR- 306
WK G LL+GPPGTGK+ L A+A K F++ ++V DS+ L K+L A
Sbjct: 34 WK-GILLFGPPGTGKTMLAKAVATECKTTFFNISASSVVSKWRGDSEKLVKVLFQLARHH 210
Query: 307 --SILVIEDIDCSVDLTERRNGGDGQTDVQAHRESDGWMQLTLSGLLNFIDGLWSSCGDE 364
S + +++ID + G+ +++ +A R + + + GL DE
Sbjct: 211 APSTIFLDEIDAIIS-----QRGEARSEHEASRRLKTELLIQMDGLTR---------TDE 348
Query: 365 RIIVF-TTNHKERLDPALLR 383
+ V TN LD A+LR
Sbjct: 349 LVFVLAATNLPWELDAAMLR 408
>TC16655 homologue to UP|O99018 (O99018) Chloroplast protease precursor,
partial (39%)
Length = 934
Score = 45.4 bits (106), Expect = 2e-05
Identities = 27/68 (39%), Positives = 38/68 (55%)
Frame = +3
Query: 218 TFETLAMEPEAKKAVMEDLNRFVKRKEFYKKVGRAWKRGYLLYGPPGTGKSSLIAAMANY 277
TF+ +A EAK+ ME + F+K+ E + VG +G LL GPPGTGK+ L A+A
Sbjct: 648 TFDDVAGVDEAKQDFMEVVE-FLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKAIAGE 824
Query: 278 LKFDIFDL 285
F +
Sbjct: 825 AGVPFFSI 848
>TC9659 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATPase,
partial (74%)
Length = 1060
Score = 45.4 bits (106), Expect = 2e-05
Identities = 51/175 (29%), Positives = 82/175 (46%), Gaps = 9/175 (5%)
Frame = +1
Query: 228 AKKAVMEDLNRFVKRKEFYKKVGRAWKRGYLLYGPPGTGKSSL---IAAMANYLKFDIFD 284
AK+++ E + VK +F+ R W R +LLYGPPGTGKS L +A A+ F +
Sbjct: 487 AKQSLQEAVILPVKFPQFFTGKRRPW-RAFLLYGPPGTGKSYLAKAVATEADSTFFSVSS 663
Query: 285 LQLGN--IVRDSDLRKLLLATANR---SILVIEDIDCSVDLTERRNGGDGQTDVQAHRES 339
LG+ + L L A SI+ +++ID L +R G+ + +A R
Sbjct: 664 SDLGSKWMGESEKLVSNLFQMARESAPSIIFVDEID---SLCGQRGEGN---ESEASRRI 825
Query: 340 DGWMQLTLSGLLNFIDGLWSSCGDERIIVF-TTNHKERLDPALLRPGRMDVHIHM 393
+ + + G+ N D++++V TN LD A+ R R D I++
Sbjct: 826 KTELLVQMQGVGN---------NDQKVLVLAATNTPYALDQAIRR--RFDKRIYI 957
>AV422700
Length = 472
Score = 44.7 bits (104), Expect = 4e-05
Identities = 18/28 (64%), Positives = 23/28 (81%)
Frame = +2
Query: 247 KKVGRAWKRGYLLYGPPGTGKSSLIAAM 274
+K+G W RG LLYGPPGTGK+SL+ A+
Sbjct: 287 RKLGLKWPRGLLLYGPPGTGKTSLVRAV 370
>CB826675
Length = 551
Score = 39.3 bits (90), Expect = 0.002
Identities = 38/128 (29%), Positives = 59/128 (45%), Gaps = 11/128 (8%)
Frame = +1
Query: 243 KEFYKKVGRAWKRGYLLYGPPGTGKSSLIAAM-----ANYLKF---DIFDLQLGN---IV 291
KE ++K+G +G LLYGPPGTGK+ + A A +LK + + +G+ +V
Sbjct: 205 KERFQKLGVRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLV 384
Query: 292 RDSDLRKLLLATANRSILVIEDIDCSVDLTERRNGGDGQTDVQAHRESDGWMQLTLSGLL 351
RD+ L + I+ I++ID G + D +Q T+ LL
Sbjct: 385 RDA---FQLAKEKSPCIIFIDEIDAI-----------GTKRFDSEVSGDREVQRTMLELL 522
Query: 352 NFIDGLWS 359
N +DG S
Sbjct: 523 NQLDGFSS 546
>TC9051 UP|PRS7_ARATH (Q9SSB5) 26S protease regulatory subunit 7 (26S
proteasome subunit 7) (26S proteasome AAA-ATPase subunit
RPT1a) (Regulatory particle triple-A ATPase subunit 1a),
partial (46%)
Length = 867
Score = 38.5 bits (88), Expect = 0.003
Identities = 20/49 (40%), Positives = 34/49 (68%)
Frame = +2
Query: 340 DGWMQLTLSGLLNFIDGLWSSCGDERIIVFTTNHKERLDPALLRPGRMD 388
D +Q T+ ++N +DG + + G+ ++++ TN + LDPALLRPGR+D
Sbjct: 164 DNEVQRTMLEIVNQLDG-FDARGNIKVLM-ATNRPDTLDPALLRPGRLD 304
>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
RPT4a, partial (49%)
Length = 648
Score = 37.7 bits (86), Expect = 0.005
Identities = 22/74 (29%), Positives = 39/74 (51%)
Frame = +2
Query: 203 YNVIKWDSINLEHPSTFETLAMEPEAKKAVMEDLNRFVKRKEFYKKVGRAWKRGYLLYGP 262
YN++ D N+ ++ + + + + E + + E + +VG +G LLYGP
Sbjct: 419 YNMLHEDPGNV----SYSAVGGLSDQIRELRESIELPLMNPELFLRVGIKPPKGVLLYGP 586
Query: 263 PGTGKSSLIAAMAN 276
PGTGK+ L A+A+
Sbjct: 587 PGTGKTLLARAIAS 628
>AV408284
Length = 425
Score = 37.7 bits (86), Expect = 0.005
Identities = 22/70 (31%), Positives = 39/70 (55%), Gaps = 8/70 (11%)
Frame = +1
Query: 254 KRGYLLYGPPGTGKSSLIAAMANYLKFDIFDLQLGNIVRD--SDLRKLLLAT------AN 305
++G LLYGPPGTGK+ L A+A ++++ N++ D +KL+ A
Sbjct: 91 QKGVLLYGPPGTGKTMLAKAIAKESGAVFINVRISNLMSKWFGDAQKLVAAVFSLAHKLQ 270
Query: 306 RSILVIEDID 315
+I+ I+++D
Sbjct: 271 PAIIFIDEVD 300
>TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), partial (32%)
Length = 1418
Score = 37.4 bits (85), Expect = 0.006
Identities = 21/56 (37%), Positives = 32/56 (56%)
Frame = +3
Query: 347 LSGLLNFIDGLWSSCGDERIIVFTTNHKERLDPALLRPGRMDVHIHMSYCSYQGFK 402
LS +L IDGL S + I+ +N + +DPALLRPGR D +++ S ++
Sbjct: 597 LSQMLAEIDGL-SDSSQDLFIIGASNRPDLIDPALLRPGRFDKLLYVGVNSDASYR 761
Score = 36.6 bits (83), Expect = 0.010
Identities = 18/49 (36%), Positives = 30/49 (60%)
Frame = +2
Query: 227 EAKKAVMEDLNRFVKRKEFYKKVGRAWKRGYLLYGPPGTGKSSLIAAMA 275
+ KK++++ + + K+ + G + G LLYGPPGTGK+ L A+A
Sbjct: 245 DVKKSILDTVQLPLLHKDLFAS-GLRKRSGVLLYGPPGTGKTLLAKAVA 388
>TC12787 weakly similar to UP|MSP1_YEAST (P28737) MSP1 protein (TAT-binding
homolog 4), partial (20%)
Length = 389
Score = 37.0 bits (84), Expect = 0.008
Identities = 28/99 (28%), Positives = 51/99 (51%), Gaps = 10/99 (10%)
Frame = +3
Query: 240 VKRKEFYKKVGRAWK--RGYLLYGPPGTGKSSLIAAMANYLKFDIFDLQLGNIV-----R 292
+KR E + K G+ K +G LL+GPPGTGK+ L A+A + ++ + +I
Sbjct: 36 LKRPELFCK-GQLTKPCKGILLFGPPGTGKTMLAKAVATEAGANFINISMSSITSKWFGE 212
Query: 293 DSDLRKLLLATANR---SILVIEDIDCSVDLTERRNGGD 328
K + + A++ S++ ++++D L R N G+
Sbjct: 213 GEKYVKAVFSLASKISPSVIFVDEVDXM--LXRRENPGE 323
>TC12379 homologue to UP|O04327 (O04327) Cell division protein FtsH isolog,
partial (15%)
Length = 505
Score = 34.7 bits (78), Expect = 0.039
Identities = 20/43 (46%), Positives = 26/43 (59%)
Frame = +1
Query: 346 TLSGLLNFIDGLWSSCGDERIIVFTTNHKERLDPALLRPGRMD 388
TL+ LL +DG E I + +TN + LDPAL+RPGR D
Sbjct: 181 TLNQLLVCLDGFEGR--GEVITIASTNRPDILDPALVRPGRFD 303
>TC14829 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (28%)
Length = 662
Score = 34.3 bits (77), Expect = 0.051
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +2
Query: 367 IVFTTNHKERLDPALLRPGRMD 388
++ TN + LDPALLRPGR+D
Sbjct: 20 VIMATNRADTLDPALLRPGRLD 85
>CN825587
Length = 663
Score = 33.9 bits (76), Expect = 0.067
Identities = 19/43 (44%), Positives = 26/43 (60%)
Frame = +2
Query: 346 TLSGLLNFIDGLWSSCGDERIIVFTTNHKERLDPALLRPGRMD 388
T++ LL +DG + G I++ TN + LD ALLRPGR D
Sbjct: 104 TINQLLTEMDGFSGNSGV--IVLAATNRPDVLDSALLRPGRFD 226
>AW719510
Length = 358
Score = 33.5 bits (75), Expect = 0.087
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = -2
Query: 367 IVFTTNHKERLDPALLRPGRMD 388
++ TN + LDPALLRPGR+D
Sbjct: 342 VLMATNRPDTLDPALLRPGRLD 277
>AV426828
Length = 401
Score = 33.5 bits (75), Expect = 0.087
Identities = 15/21 (71%), Positives = 17/21 (80%)
Frame = +1
Query: 258 LLYGPPGTGKSSLIAAMANYL 278
LLYGPPGTGK+S I A+A L
Sbjct: 121 LLYGPPGTGKTSTILAVARKL 183
>TC11760 homologue to UP|PRS4_ORYSA (P46466) 26S protease regulatory subunit
4 homolog (TAT-binding protein homolog 2), partial (27%)
Length = 555
Score = 32.7 bits (73), Expect = 0.15
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +3
Query: 362 GDERIIVFTTNHKERLDPALLRPGRMD 388
GD ++I+ T+ E LDPALLRPGR+D
Sbjct: 6 GDVKVIL-ATHSIESLDPALLRPGRID 83
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,117,131
Number of Sequences: 28460
Number of extensions: 102297
Number of successful extensions: 611
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 605
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 608
length of query: 521
length of database: 4,897,600
effective HSP length: 94
effective length of query: 427
effective length of database: 2,222,360
effective search space: 948947720
effective search space used: 948947720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0249.15