BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0248.1
(119 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780221 45 3e-06
BP060613 43 1e-05
AV767121 39 2e-04
AV780705 33 0.013
TC14879 27 0.70
AV779048 26 2.0
TC8475 similar to UP|GAL1_ARATH (Q9SEE5) Galactokinase (Galacto... 25 2.6
TC13681 similar to UP|ASPG_LUPAL (P50288) L-asparaginase (L-asp... 25 3.5
TC12281 homologue to UP|Q949G4 (Q949G4) N3 like protein, partial... 25 3.5
BP081719 25 4.5
AV769794 25 4.5
BP075017 24 5.9
TC13263 similar to UP|Q9M5L9 (Q9M5L9) Mismatch binding protein M... 24 5.9
AV412627 24 5.9
TC13982 24 5.9
BP065867 24 5.9
TC9881 24 5.9
BP067224 24 7.7
TC9986 similar to UP|Q9M427 (Q9M427) Oligouridylate binding prot... 24 7.7
TC8625 similar to UP|O23062 (O23062) CODED for BY A. THALIANA CD... 24 7.7
>AV780221
Length = 461
Score = 45.1 bits (105), Expect = 3e-06
Identities = 24/61 (39%), Positives = 37/61 (60%)
Frame = +3
Query: 33 KGGQTHSWYLVPWCEVSCPKENGGLGLRSTSLNNIAMLEKLVESLLRDKGKLWGRVLSQK 92
+GG+ +W V W V PK+ GGLG+++ L N A+L K +L+++ LW +VL K
Sbjct: 129 EGGKKIAW--VKWSTVCRPKDEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIK 302
Query: 93 Y 93
Y
Sbjct: 303 Y 305
>BP060613
Length = 378
Score = 43.1 bits (100), Expect = 1e-05
Identities = 21/90 (23%), Positives = 40/90 (44%)
Frame = +1
Query: 30 IWAKGGQTHSWYLVPWCEVSCPKENGGLGLRSTSLNNIAMLEKLVESLLRDKGKLWGRVL 89
+W T + W ++ K+ GGLG + + N A+L K +L + LW ++L
Sbjct: 43 VWWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQIL 222
Query: 90 SQKYLSNKSVLAGKYKRGDSYIWKGIVSAK 119
Y + L + G S++W ++ +
Sbjct: 223 KALYFPHHDFLQTTKRTGASWVWSSLLHGR 312
>AV767121
Length = 525
Score = 38.9 bits (89), Expect = 2e-04
Identities = 31/115 (26%), Positives = 49/115 (41%)
Frame = +2
Query: 3 SLPLLEKNEFTFKLHLHLEYDKNIRSCIWAKGGQTHSWYLVPWCEVSCPKENGGLGLRST 62
+LPL + F + + + +R+ +W + V W +V PKE GGLG++
Sbjct: 71 ALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENKIA*VKWTDVCKPKELGGLGIKDL 250
Query: 63 SLNNIAMLEKLVESLLRDKGKLWGRVLSQKYLSNKSVLAGKYKRGDSYIWKGIVS 117
N A+L K L + LW RV+ + Y G S+ W I+S
Sbjct: 251 FTFNKALLGK*RWRYLTEPDSLWRRVIEAQ--------PDHYSCGSSW-WNDILS 388
>AV780705
Length = 524
Score = 33.1 bits (74), Expect = 0.013
Identities = 16/62 (25%), Positives = 28/62 (44%)
Frame = -2
Query: 52 KENGGLGLRSTSLNNIAMLEKLVESLLRDKGKLWGRVLSQKYLSNKSVLAGKYKRGDSYI 111
KE GG+G R N+A L + +L + LW RV+ Y ++ + ++
Sbjct: 187 KEKGGVGFRDLRTQNLAFLARQAWRVLTNPEALWVRVMKSLYFPTPALCLLLWDGTHPWM 8
Query: 112 WK 113
W+
Sbjct: 7 WR 2
>TC14879
Length = 1021
Score = 27.3 bits (59), Expect = 0.70
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = +3
Query: 27 RSCIWAKGGQTHSWYLVPWCEVSCPKENG 55
R C W + G+ +SW+ PWC V + G
Sbjct: 330 RLCWWLRLGRINSWH--PWCCVCTSDQQG 410
>AV779048
Length = 531
Score = 25.8 bits (55), Expect = 2.0
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +3
Query: 7 LEKNEFTFKLHLHLEYDKNIRSCIWAK 33
+ N F+ + HLH+ D +RS IW K
Sbjct: 207 IRTNLFSNRYHLHMFADPILRSRIWIK 287
>TC8475 similar to UP|GAL1_ARATH (Q9SEE5) Galactokinase (Galactose kinase) ,
partial (86%)
Length = 1662
Score = 25.4 bits (54), Expect = 2.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1
Query: 64 LNNIAMLEKLVESLLRDKGKLWGRVL 89
LNN + +LVE+ L+ K KLW L
Sbjct: 1488 LNNALVHPRLVETFLQIKDKLWDYAL 1411
>TC13681 similar to UP|ASPG_LUPAL (P50288) L-asparaginase (L-asparagine
amidohydrolase) , partial (35%)
Length = 615
Score = 25.0 bits (53), Expect = 3.5
Identities = 15/48 (31%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = -2
Query: 27 RSCIWAKGGQTHSWYLVPWCEVSCPKENGGLGLRSTSL-NNIAMLEKL 73
+ C+WA H+W L + P E L LR+ L ++ L+KL
Sbjct: 227 QQCLWACARAQHNWQLQ*EKDPGIPSEQPHLWLRARVLWIHLGRLQKL 84
>TC12281 homologue to UP|Q949G4 (Q949G4) N3 like protein, partial (22%)
Length = 488
Score = 25.0 bits (53), Expect = 3.5
Identities = 7/18 (38%), Positives = 12/18 (65%)
Frame = -3
Query: 14 FKLHLHLEYDKNIRSCIW 31
+++H HL Y K+ C+W
Sbjct: 141 YQIHHHLNYAKHKAKCVW 88
>BP081719
Length = 374
Score = 24.6 bits (52), Expect = 4.5
Identities = 9/17 (52%), Positives = 14/17 (81%)
Frame = -1
Query: 52 KENGGLGLRSTSLNNIA 68
++NGGL L +SLNN++
Sbjct: 311 EDNGGLNLSRSSLNNVS 261
>AV769794
Length = 559
Score = 24.6 bits (52), Expect = 4.5
Identities = 10/31 (32%), Positives = 12/31 (38%), Gaps = 7/31 (22%)
Frame = +1
Query: 27 RSCIWAKGGQTHSWYLVPW-------CEVSC 50
R +W G H W L+ W CE C
Sbjct: 355 RRAVWINSGVKHGWCLLRWTGGGALLCE*KC 447
>BP075017
Length = 443
Score = 24.3 bits (51), Expect = 5.9
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +2
Query: 96 NKSVLAGKYKRGDSYIWKGI 115
++S LAGK+K DSY W+G+
Sbjct: 308 SRSFLAGKFKILDSY-WEGL 364
>TC13263 similar to UP|Q9M5L9 (Q9M5L9) Mismatch binding protein Mus3
(Fragment), partial (5%)
Length = 599
Score = 24.3 bits (51), Expect = 5.9
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 1 IGSLPLLEKNEFTFKLHLHLEYDKNI 26
+GS+ L+KNE F L +DKN+
Sbjct: 365 VGSIFALQKNELPFS*LTSLLFDKNL 288
>AV412627
Length = 326
Score = 24.3 bits (51), Expect = 5.9
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = -2
Query: 69 MLEKLVESLLRDKGKLWGRVLSQKYLSNKSVLAGKYKRGDSYIWK 113
+L +LVE L D + W +V +K + ++ L + G S WK
Sbjct: 277 ILLRLVEESLADTARRWSKVSERK--TEENALFFGEEEGGSMKWK 149
>TC13982
Length = 551
Score = 24.3 bits (51), Expect = 5.9
Identities = 6/14 (42%), Positives = 10/14 (70%)
Frame = +2
Query: 42 LVPWCEVSCPKENG 55
L+PWC+ +C + G
Sbjct: 14 LIPWCKCACSRRRG 55
>BP065867
Length = 524
Score = 24.3 bits (51), Expect = 5.9
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1
Query: 10 NEFTFKLHLHLEYDKNIRSCIW 31
N F+ + HLH+ D +RS IW
Sbjct: 370 NLFSNRYHLHMFDDPILRSMIW 435
>TC9881
Length = 559
Score = 24.3 bits (51), Expect = 5.9
Identities = 19/60 (31%), Positives = 29/60 (47%), Gaps = 6/60 (10%)
Frame = -1
Query: 39 SWYLVPWCEVSC---PKENGG---LGLRSTSLNNIAMLEKLVESLLRDKGKLWGRVLSQK 92
SWY + CEV C P G LG +TS N A +K + + ++ + W ++L K
Sbjct: 352 SWYTLQLCEVGCTCSP*NISGIESLGTWATS-TNPASSQKCQKIMFLERNQ-WTKILDHK 179
>BP067224
Length = 348
Score = 23.9 bits (50), Expect = 7.7
Identities = 10/28 (35%), Positives = 14/28 (49%)
Frame = +2
Query: 91 QKYLSNKSVLAGKYKRGDSYIWKGIVSA 118
+KY +L GK K G + WK + A
Sbjct: 221 KKYDGGLKILLGKIKLGAGFSWKSLGKA 304
>TC9986 similar to UP|Q9M427 (Q9M427) Oligouridylate binding protein, partial
(46%)
Length = 1150
Score = 23.9 bits (50), Expect = 7.7
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -1
Query: 3 SLPLLEKNEFTFKLHLHLEYDKNIR 27
SLP LEK+ + F H L D+ I+
Sbjct: 1075 SLPFLEKHRYLFWSHQTLGVDQTIQ 1001
>TC8625 similar to UP|O23062 (O23062) CODED for BY A. THALIANA CDNA N96903,
partial (59%)
Length = 844
Score = 23.9 bits (50), Expect = 7.7
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +3
Query: 30 IWAKGGQTHSWYLVPWCEVS 49
+W +GG TH + + +C +S
Sbjct: 756 LWGEGGYTHPFMSLIFCSIS 815
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.137 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,650,536
Number of Sequences: 28460
Number of extensions: 39743
Number of successful extensions: 197
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of query: 119
length of database: 4,897,600
effective HSP length: 79
effective length of query: 40
effective length of database: 2,649,260
effective search space: 105970400
effective search space used: 105970400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)
Lotus: description of TM0248.1