BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0247.8
(170 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV424663 40 3e-04
AV777083 34 0.012
BP052474 30 0.17
TC12470 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (5%) 28 0.64
TC7852 similar to UP|Q9ZP07 (Q9ZP07) Ribulose 1,5-bisphosphate c... 27 1.8
BP030905 23 2.2
TC9806 similar to UP|Q9FJA0 (Q9FJA0) Gb|AAD21756.1, partial (13%) 27 2.4
TC11570 similar to UP|O22165 (O22165) 60S ribosomal protein L30 ... 26 3.2
TC12293 weakly similar to UP|AAR10436 (AAR10436) YDA, partial (3%) 26 3.2
TC9929 similar to UP|O81396 (O81396) Aconitase-iron regulated pr... 26 3.2
TC9464 25 7.0
TC14273 similar to UP|DCE1_ARATH (Q42521) Glutamate decarboxylas... 25 7.0
TC15123 similar to UP|AAM51564 (AAM51564) Flavonoid 3', 5'-hydro... 25 7.0
BP032314 25 9.2
TC11348 homologue to UP|MSI1_LYCES (O22466) WD-40 repeat protein... 25 9.2
TC9072 weakly similar to GB|AAP37737.1|30725430|BT008378 At1g707... 25 9.2
TC10582 25 9.2
>AV424663
Length = 427
Score = 39.7 bits (91), Expect = 3e-04
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = +2
Query: 16 SLDTSPRVLAKLMSLKAYPKV 36
SLDTSPRVL KL SLKAYPKV
Sbjct: 152 SLDTSPRVLGKLKSLKAYPKV 214
>AV777083
Length = 369
Score = 34.3 bits (77), Expect = 0.012
Identities = 21/48 (43%), Positives = 25/48 (51%), Gaps = 6/48 (12%)
Frame = -1
Query: 127 NILRTYQTPQHLDPTTLLVF------PKLWFDFQCLPSRLLSLLKDFE 168
+IL Q QH DPTTLLVF PKL F+F + R L +E
Sbjct: 315 HILNASQFSQHSDPTTLLVFQN*DLQPKLPFEFVIIT*RFRGCLMSYE 172
>BP052474
Length = 504
Score = 30.4 bits (67), Expect = 0.17
Identities = 18/51 (35%), Positives = 27/51 (52%)
Frame = -1
Query: 54 TMTLRVINTLTKSHASVKFHALTRSHASVEFHAFHKVSRLSGVSSIHKVSR 104
T +++ + K H + H H +V+ H+F KVS L VSSI +SR
Sbjct: 486 TFVIQIXPVVRKXHLARNXHF--SKHLTVKSHSFXKVSYLFIVSSISFISR 340
>TC12470 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (5%)
Length = 825
Score = 28.5 bits (62), Expect = 0.64
Identities = 18/68 (26%), Positives = 32/68 (46%)
Frame = -1
Query: 70 VKFHALTRSHASVEFHAFHKVSRLSGVSSIHKVSRLSEASRLIPWMANKLLNERRSTNIL 129
+K HA + H+ + + AFH R S + + L E+ WM L R+ ++
Sbjct: 363 IK*HAYRQQHSGLSYLAFHDQYR-SIFTRLESSIPLRES-----WMVKNLEQHLRNLSLA 202
Query: 130 RTYQTPQH 137
+ Q+P+H
Sbjct: 201 SSTQSPEH 178
>TC7852 similar to UP|Q9ZP07 (Q9ZP07) Ribulose 1,5-bisphosphate carboxylase
small subunit precursor , partial (98%)
Length = 847
Score = 26.9 bits (58), Expect = 1.8
Identities = 24/89 (26%), Positives = 38/89 (41%)
Frame = +3
Query: 54 TMTLRVINTLTKSHASVKFHALTRSHASVEFHAFHKVSRLSGVSSIHKVSRLSEASRLIP 113
TMTL + T + + + + L +S FH FH + +S K + S +
Sbjct: 153 TMTLPPLQTTVEEYNACRCGLLLARRSSRLFHTFHHSPQ---SNS*RK*NTFSGRGGFLA 323
Query: 114 WMANKLLNERRSTNILRTYQTPQHLDPTT 142
W L +R++ L T +T HLD T
Sbjct: 324 WS----LRQRKA---LSTVRTTSHLDTMT 389
>BP030905
Length = 484
Score = 22.7 bits (47), Expect(2) = 2.2
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +1
Query: 135 PQHLDPTTLLVFPKLWFDFQCLPSRLLSLLKDFEVI 170
PQ+LD TLL F L+ + ++ +LK +V+
Sbjct: 142 PQNLDSATLLFFQNLFPSPKLPLESVIIILKCLDVV 249
Score = 22.3 bits (46), Expect(2) = 2.2
Identities = 9/17 (52%), Positives = 13/17 (75%)
Frame = +2
Query: 113 PWMANKLLNERRSTNIL 129
P KLL+E++STN+L
Sbjct: 5 PSDGQKLLSEKKSTNVL 55
>TC9806 similar to UP|Q9FJA0 (Q9FJA0) Gb|AAD21756.1, partial (13%)
Length = 546
Score = 26.6 bits (57), Expect = 2.4
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = +3
Query: 121 NERRSTNILRTYQTPQHLDPTTLLVFPKLWFDFQCLPSRLLSL 163
+ + S NI+ +Q H+ + F + F CLP+ LSL
Sbjct: 309 HSKHSGNIVVVFQA*MHVQCCVIFYFDLNFSPFTCLPAPFLSL 437
>TC11570 similar to UP|O22165 (O22165) 60S ribosomal protein L30
(At2g44860/T13E15.13), partial (64%)
Length = 1282
Score = 26.2 bits (56), Expect = 3.2
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = -3
Query: 7 VISQTTSPTSLDTSPRVLAKLM 28
++ T PTSLD +P+V K++
Sbjct: 1076 IVCNLTEPTSLDNTPKVCPKIL 1011
>TC12293 weakly similar to UP|AAR10436 (AAR10436) YDA, partial (3%)
Length = 637
Score = 26.2 bits (56), Expect = 3.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = -1
Query: 138 LDPTTLLVFPKLWFDFQCLPSRLLS 162
LDP L+ + +F+F+C+ S++LS
Sbjct: 460 LDPPDFLLSLEAFFNFRCIASKILS 386
>TC9929 similar to UP|O81396 (O81396) Aconitase-iron regulated protein 1,
partial (10%)
Length = 583
Score = 26.2 bits (56), Expect = 3.2
Identities = 26/87 (29%), Positives = 37/87 (41%)
Frame = -2
Query: 35 KVKSILRQNTIIIKRVQPLTMTLRVINTLTKSHASVKFHALTRSHASVEFHAFHKVSRLS 94
K K +R N +I V + R I+ LT++ FH + H F + LS
Sbjct: 429 KEKYGIRNNKLIDDSV---SKNKRKISWLTETG*VFPFHGII-------IHMFQYTALLS 280
Query: 95 GVSSIHKVSRLSEASRLIPWMANKLLN 121
VS HK L +L+ WMA L+
Sbjct: 279 RVS**HKEVCLHSPVQLLQWMAKHHLS 199
>TC9464
Length = 567
Score = 25.0 bits (53), Expect = 7.0
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -1
Query: 61 NTLTKSHASVKFHALTRSHASVEFH 85
N + ++A V+ H +HAS+ FH
Sbjct: 270 NVIPLAYARVRVHRFLEAHASMAFH 196
>TC14273 similar to UP|DCE1_ARATH (Q42521) Glutamate decarboxylase 1 (GAD
1) , partial (89%)
Length = 1826
Score = 25.0 bits (53), Expect = 7.0
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 2 SACNAVISQTTSPTSLDTSPRVLAK 26
S+ + IS+ +P S TSP+VLAK
Sbjct: 964 SSSISTISELINPPSPSTSPKVLAK 1038
>TC15123 similar to UP|AAM51564 (AAM51564) Flavonoid 3', 5'-hydroxylase,
partial (24%)
Length = 592
Score = 25.0 bits (53), Expect = 7.0
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = -3
Query: 118 KLLNERRSTNILRTYQTPQHL 138
+LLN RR I+R +Q+PQ++
Sbjct: 275 QLLNTRRQLPIIRMHQSPQNV 213
>BP032314
Length = 426
Score = 24.6 bits (52), Expect = 9.2
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -2
Query: 70 VKFHALTRSHASVEFHAFHKVSRL 93
+ FH+LTR A + + F ++ RL
Sbjct: 287 LSFHSLTRESAFIHVNIFKRIERL 216
>TC11348 homologue to UP|MSI1_LYCES (O22466) WD-40 repeat protein MSI1,
partial (16%)
Length = 547
Score = 24.6 bits (52), Expect = 9.2
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = -3
Query: 123 RRSTNILRTYQTPQHLDP---TTLLV 145
+RSTN + Y TP+H P TTL +
Sbjct: 323 KRSTNTNKKYPTPKHSTPIFQTTLTI 246
>TC9072 weakly similar to GB|AAP37737.1|30725430|BT008378 At1g70770
{Arabidopsis thaliana;}, partial (6%)
Length = 584
Score = 24.6 bits (52), Expect = 9.2
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = -3
Query: 129 LRTYQTPQHLDPTTLLVFPKLWFDFQC 155
+ Y Q P T+ +F WFDF C
Sbjct: 402 INNYVNGQRKLPWTMALFLLRWFDFTC 322
>TC10582
Length = 382
Score = 24.6 bits (52), Expect = 9.2
Identities = 9/17 (52%), Positives = 10/17 (57%)
Frame = +1
Query: 145 VFPKLWFDFQCLPSRLL 161
+FP WF F CL R L
Sbjct: 136 IFPSTWFRFNCLGVRSL 186
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,897,333
Number of Sequences: 28460
Number of extensions: 36485
Number of successful extensions: 230
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of query: 170
length of database: 4,897,600
effective HSP length: 84
effective length of query: 86
effective length of database: 2,506,960
effective search space: 215598560
effective search space used: 215598560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0247.8