BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0239.15
(483 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV422763 101 2e-22
AV427280 66 1e-11
AV410889 57 9e-09
AU089369 53 1e-07
AV421795 48 4e-06
TC10842 44 6e-05
AU251624 40 6e-04
TC17100 similar to UP|MID2_YEAST (P36027) Mating process protein... 38 0.003
TC15322 weakly similar to UP|Q9LG23 (Q9LG23) F14J16.14 (At1g5589... 38 0.003
BP047039 37 0.005
AV772727 35 0.021
AV417736 32 0.23
BP041405 31 0.51
TC18432 similar to UP|Q8RX69 (Q8RX69) At1g15070/F9L1_1, partial ... 28 2.5
BP040806 28 2.5
BP054863 28 3.3
TC12677 similar to UP|Q9FQD6 (Q9FQD6) Glutathione S-transferase ... 28 3.3
BF177625 27 7.4
AU089121 27 9.7
>AV422763
Length = 410
Score = 101 bits (252), Expect = 2e-22
Identities = 55/137 (40%), Positives = 85/137 (61%), Gaps = 1/137 (0%)
Frame = +2
Query: 188 VHDMKERNIPMSAFAYHAWMNSYAALNDLVGVEKIYELMKLEVETKIDWLTYSNLAAIYV 247
+ +MKE ++ A + +N+Y A D+ G+EK+ M+ + +DW TYS A Y+
Sbjct: 2 MQEMKENDLCNGA-TFTIRLNAYVAAKDVEGMEKLLMQMEADPMATVDWYTYSTAANGYL 178
Query: 248 KAKQFEKAELMLRKLEQAVKPRK-REAYHCLLSLYAGTGNLEKVYSLWDSLKSVTPVLNV 306
KA EKA L+K EQ+VK +K R AY L S YA GN ++VY LW+ +K++ N
Sbjct: 179 KAGNVEKALAALKKSEQSVKGKKLRLAYESLQSTYAAIGNNDEVYRLWNRIKNLPNCWNS 358
Query: 307 SYLCMISALKRLNDMEG 323
SY+CM+S+L +L+D++G
Sbjct: 359 SYICMLSSLLKLDDLDG 409
Score = 32.3 bits (72), Expect = 0.18
Identities = 29/97 (29%), Positives = 45/97 (45%), Gaps = 3/97 (3%)
Frame = +2
Query: 125 PPLAMNKYTYGALLNCYCKELMVNKALAHFDKMNE--LGYVTFLSFNNLIGMYMRLGQPL 182
P ++ YTY N Y K V KALA K + G L++ +L Y +G
Sbjct: 125 PMATVDWYTYSTAANGYLKAGNVEKALAALKKSEQSVKGKKLRLAYESLQSTYAAIGNND 304
Query: 183 KVHELVHDMKERNIPMS-AFAYHAWMNSYAALNDLVG 218
+V+ L + +K N+P +Y ++S L+DL G
Sbjct: 305 EVYRLWNRIK--NLPNCWNSSYICMLSSLLKLDDLDG 409
>AV427280
Length = 268
Score = 65.9 bits (159), Expect = 1e-11
Identities = 32/88 (36%), Positives = 54/88 (61%)
Frame = +3
Query: 149 KALAHFDKMNELGYVTFLSFNNLIGMYMRLGQPLKVHELVHDMKERNIPMSAFAYHAWMN 208
++L+ KM E+GYV+ L++NN++ +Y + Q KV ++ MKE + F+Y +N
Sbjct: 3 ESLSQMQKMKEMGYVSCLNYNNIMCLYAQSDQHEKVPGVLAMMKEDGVSPDIFSYRVCIN 182
Query: 209 SYAALNDLVGVEKIYELMKLEVETKIDW 236
SY A +DL +EK+ E ++ E T +DW
Sbjct: 183 SYGARSDLENMEKLLEEIEREGHTCVDW 266
>AV410889
Length = 436
Score = 56.6 bits (135), Expect = 9e-09
Identities = 30/90 (33%), Positives = 49/90 (54%), Gaps = 1/90 (1%)
Frame = +1
Query: 32 SLYQRLSELEKTGMSASQTLDQYISEGKVAGKKELMSIVKELRKYRRFQDALQIMKWMES 91
+L+ R+S + + L+Q++ EG+ +L +K+LR +RRF+ ALQI +WM
Sbjct: 166 TLFLRISRTGDPAIPVTPLLNQWVQEGRPINHGDLQFFIKQLRSFRRFKHALQISEWMSD 345
Query: 92 RVESPL-DYNYAVHLDLVCKNEGVVAAENY 120
L + A+ LDL+ K G+ AE Y
Sbjct: 346 ERNHYLHSGDIAIRLDLISKVRGLEEAEKY 435
>AU089369
Length = 728
Score = 53.1 bits (126), Expect = 1e-07
Identities = 31/104 (29%), Positives = 56/104 (53%), Gaps = 2/104 (1%)
Frame = +3
Query: 339 DLRLISAVISAYLSHDMSEEAALVFKDAIKKSKGPFFKIREMFMIFFLEKCQLDGAVSHL 398
D+R+ + +I Y DM ++A + + A ++ P K E+F+ + L K AV L
Sbjct: 24 DIRVANVLIGTYAKLDMLDKAEELKERARRRGAKPNAKTWEIFLDYHLRKGDFKLAVDSL 203
Query: 399 EAALS--EVVGDEWRPTPRVLRAFVKYYKEETDLDGVDELSKIL 440
+ A+S GD+W P+ + +K++++E D+DG E +IL
Sbjct: 204 DKAISIGRGNGDKWVPSSESVGTIMKHFEQEKDVDGAXEFLEIL 335
>AV421795
Length = 267
Score = 47.8 bits (112), Expect = 4e-06
Identities = 23/60 (38%), Positives = 35/60 (58%)
Frame = +2
Query: 32 SLYQRLSELEKTGMSASQTLDQYISEGKVAGKKELMSIVKELRKYRRFQDALQIMKWMES 91
SL+ R+S + + L+Q++ EGK+ EL +K+LR RRF ALQI +WM +
Sbjct: 65 SLFLRISRSGDRAIPMTPLLNQWLQEGKLINYSELQFFIKQLRASRRFNHALQISEWMSN 244
>TC10842
Length = 597
Score = 43.9 bits (102), Expect = 6e-05
Identities = 27/116 (23%), Positives = 60/116 (51%), Gaps = 1/116 (0%)
Frame = +2
Query: 259 LRKLEQAVKPRKREAYHCLLSLYAGTGNLEKVYSLWDSLK-SVTPVLNVSYLCMISALKR 317
L + E+ + + Y L+ LYAG G+ +K+ +W+SL+ + ++N +Y C+ISA
Sbjct: 11 LAETEKRITQSQWITYDFLIILYAGLGSKDKLDQIWNSLRMTKQKMINRNYSCIISAYLM 190
Query: 318 LNDMEGLIRCFKEWESRYGHCDLRLISAVISAYLSHDMSEEAALVFKDAIKKSKGP 373
L ++ + +W++ D+ ++ A+ + +++ A + I+K+ P
Sbjct: 191 LGHVKEVGEVIDQWKNSTIDFDMLACKKIMDAFRATGLADIANNLNMILIEKNINP 358
Score = 33.9 bits (76), Expect = 0.061
Identities = 17/70 (24%), Positives = 32/70 (45%)
Frame = +2
Query: 232 TKIDWLTYSNLAAIYVKAKQFEKAELMLRKLEQAVKPRKREAYHCLLSLYAGTGNLEKVY 291
T+ W+TY L +Y +K + + L + Y C++S Y G++++V
Sbjct: 35 TQSQWITYDFLIILYAGLGSKDKLDQIWNSLRMTKQKMINRNYSCIISAYLMLGHVKEVG 214
Query: 292 SLWDSLKSVT 301
+ D K+ T
Sbjct: 215 EVIDQWKNST 244
>AU251624
Length = 350
Score = 40.4 bits (93), Expect = 6e-04
Identities = 24/79 (30%), Positives = 44/79 (55%)
Frame = +1
Query: 32 SLYQRLSELEKTGMSASQTLDQYISEGKVAGKKELMSIVKELRKYRRFQDALQIMKWMES 91
+LY +L + + ++ Q L+Q+I GK K E+ +K+LR+ + + AL++ + M
Sbjct: 109 TLYLKLFKDGGSDLNVRQQLNQFIKSGKRVYKWEVGDTLKKLRQRKLYVPALKLSETMAK 288
Query: 92 RVESPLDYNYAVHLDLVCK 110
R + A+HLDL+ K
Sbjct: 289 RNMIKTVSDQAIHLDLLAK 345
>TC17100 similar to UP|MID2_YEAST (P36027) Mating process protein MID2
(Serine-rich protein SMS1) (Protein kinase A
interference protein), partial (7%)
Length = 704
Score = 38.1 bits (87), Expect = 0.003
Identities = 22/107 (20%), Positives = 52/107 (48%), Gaps = 1/107 (0%)
Frame = +3
Query: 330 EWESRYGHCDLRLISAVISAYLSHDMSEEAALVFKDAIKKSKGPFFKIREMFMIFFLEKC 389
EWE D R+ + ++ Y + E+A + + ++K K P + ++ +
Sbjct: 24 EWELSGNTYDFRVPNILLIGYSKKGLIEKAETMLRSMVEKGKTPNPYSWSIVASGYVAQE 203
Query: 390 QLDGAVSHLEAALSEVVGDE-WRPTPRVLRAFVKYYKEETDLDGVDE 435
++ A L+ AL+ + ++ WRP P V+ + + + + D++ V++
Sbjct: 204 NMEKAFQCLKEALAVLAENKGWRPKPNVVSSILSWVTDNRDIEEVED 344
Score = 27.7 bits (60), Expect = 4.3
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 5/73 (6%)
Frame = +3
Query: 224 ELMKLEVETKIDWLTYS----NLAAI-YVKAKQFEKAELMLRKLEQAVKPRKREAYHCLL 278
E KL E ++ TY N+ I Y K EKAE MLR + + K ++ +
Sbjct: 3 EAEKLLGEWELSGNTYDFRVPNILLIGYSKKGLIEKAETMLRSMVEKGKTPNPYSWSIVA 182
Query: 279 SLYAGTGNLEKVY 291
S Y N+EK +
Sbjct: 183 SGYVAQENMEKAF 221
>TC15322 weakly similar to UP|Q9LG23 (Q9LG23) F14J16.14
(At1g55890/F14J16_4), partial (33%)
Length = 588
Score = 38.1 bits (87), Expect = 0.003
Identities = 18/72 (25%), Positives = 39/72 (54%)
Frame = +2
Query: 171 LIGMYMRLGQPLKVHELVHDMKERNIPMSAFAYHAWMNSYAALNDLVGVEKIYELMKLEV 230
LI +Y + G P + ++ +M ERN + +++A + +Y VE+I++ + E+
Sbjct: 359 LITLYGKSGMPKRARQMFDEMPERNCTRTVLSFNALLAAYLHSKQYRVVERIFKEVPGEI 538
Query: 231 ETKIDWLTYSNL 242
K D ++Y+ +
Sbjct: 539 SVKPDLVSYNTV 574
>BP047039
Length = 527
Score = 37.4 bits (85), Expect = 0.005
Identities = 19/69 (27%), Positives = 37/69 (53%), Gaps = 1/69 (1%)
Frame = -3
Query: 129 MNKYTYGALLNCYCKELMVNKALAHFDKMNELGYV-TFLSFNNLIGMYMRLGQPLKVHEL 187
+N +TY ++N CKE + ++AL KM + G + ++F +I G+ K +L
Sbjct: 501 LNNWTYNTMINGLCKEGLFDEALTLMSKMEDNGCIHDPITFETIISALFEKGENDKAEKL 322
Query: 188 VHDMKERNI 196
+ +M R++
Sbjct: 321 LREMIARDL 295
>AV772727
Length = 528
Score = 35.4 bits (80), Expect = 0.021
Identities = 23/87 (26%), Positives = 43/87 (48%), Gaps = 2/87 (2%)
Frame = -2
Query: 68 SIVKELRKYRRFQDALQIMKWMESRVESPLDYNYAVHLDLVCKNEGVVAAENYFNSL--P 125
S++ L K A+ +++ ++ + P Y Y + +D +CK+ V A+ F L
Sbjct: 512 SLLDALCKNHHVDKAIALLERVKDKGIQPDMYTYNIIIDGLCKSGRVKDAQELFQDLLIK 333
Query: 126 PLAMNKYTYGALLNCYCKELMVNKALA 152
+N TY ++N C E + ++ALA
Sbjct: 332 GYRLNVVTYNIMINGLCIEGLSDEALA 252
>AV417736
Length = 428
Score = 32.0 bits (71), Expect = 0.23
Identities = 23/90 (25%), Positives = 43/90 (47%)
Frame = +1
Query: 175 YMRLGQPLKVHELVHDMKERNIPMSAFAYHAWMNSYAALNDLVGVEKIYELMKLEVETKI 234
Y+R GQP K L+ M++ + + + ++ + + +++E M E+
Sbjct: 85 YVRSGQPGKAEALLTSMRKYGLQPNVVIFTTIISGWCTAGKMDRAVRLHEKMH-EMGIPS 261
Query: 235 DWLTYSNLAAIYVKAKQFEKAELMLRKLEQ 264
+ TY L Y +AKQ KAE +L +E+
Sbjct: 262 NLKTYETLMWGYGEAKQPWKAEELLVTMEE 351
>BP041405
Length = 546
Score = 30.8 bits (68), Expect = 0.51
Identities = 28/121 (23%), Positives = 56/121 (46%), Gaps = 5/121 (4%)
Frame = +3
Query: 81 DALQIMKWMESRVESPLDYNYAVHLDLVCKNE----GVVAAENYFNSLPPLAMNKYTYGA 136
D + + + ME +P Y AV ++ +CKN G + + P+ + Y Y A
Sbjct: 162 DVVHVFQEMEEAGVTPDSYCNAVLIEGLCKNHRSDWGYQFLQEFRKVNAPIEV--YAYTA 335
Query: 137 LLNCYCKELMVNKALAHFDKMNELGYVTFLS-FNNLIGMYMRLGQPLKVHELVHDMKERN 195
+++ +C E+ +++A + M G V ++ ++ LI Y + + +L DM +
Sbjct: 336 VIHGFCNEMKLDEAESVVLDMERQGLVPDVNIYSALICGYCKSHNLPRALDLYADMISKG 515
Query: 196 I 196
I
Sbjct: 516 I 518
>TC18432 similar to UP|Q8RX69 (Q8RX69) At1g15070/F9L1_1, partial (6%)
Length = 769
Score = 28.5 bits (62), Expect = 2.5
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +3
Query: 310 CMISALKRLNDMEGLIRCFKEWESRYGHCDL 340
C+I+ L +++ LIR F W + HC L
Sbjct: 162 CLITTLIPTENLKELIRAFMYWSMTFSHCFL 254
>BP040806
Length = 534
Score = 28.5 bits (62), Expect = 2.5
Identities = 25/112 (22%), Positives = 48/112 (42%), Gaps = 2/112 (1%)
Frame = +1
Query: 133 TYGALLNCYCKELMVNKALAHFDKM--NELGYVTFLSFNNLIGMYMRLGQPLKVHELVHD 190
TY ALL YC+ ++++A + ++M + L +++ LI + + V L D
Sbjct: 199 TYTALLAAYCRSNLLDEAFSILNEMKNHPLCQPDVFTYSTLIKPCVDAFKFDLVELLYED 378
Query: 191 MKERNIPMSAFAYHAWMNSYAALNDLVGVEKIYELMKLEVETKIDWLTYSNL 242
M R+I + + ++ Y +EK+ M K D T + +
Sbjct: 379 MAARSIMPNTVTQNIVLSGYGKAGKFDQMEKVLSSMLESTSCKPDVWTMNTI 534
>BP054863
Length = 498
Score = 28.1 bits (61), Expect = 3.3
Identities = 27/94 (28%), Positives = 40/94 (41%), Gaps = 1/94 (1%)
Frame = +3
Query: 45 MSASQTLDQYISEGKVAGKKELMSIVKELRKYRRFQDALQIMKWMESRVESPLDYNYAVH 104
++A + LD +S +V +E I +R+Y + + W E N V+
Sbjct: 72 LTAIE*LDFKLSSKRVT*HRERRFIFSWVRQYSSSHACI*NLIWKE--------LNKTVN 227
Query: 105 LDLVCKNEGVVAAENYFNSL-PPLAMNKYTYGAL 137
L VCK V + NYF L P L N T +L
Sbjct: 228 LFFVCKFSCFVVSANYFPLLIPALYRNSTTIFSL 329
>TC12677 similar to UP|Q9FQD6 (Q9FQD6) Glutathione S-transferase GST 22
(Fragment) , complete
Length = 876
Score = 28.1 bits (61), Expect = 3.3
Identities = 14/51 (27%), Positives = 24/51 (46%)
Frame = +3
Query: 418 RAFVKYYKEETDLDGVDELSKILMTHNFDDSWLQSSIAAAESSPEIYPILN 468
RA ++YY E+ G + L K + + WL + A P I+ ++N
Sbjct: 228 RAIIRYYAEKYKNQGTELLGKTIEERGLVEQWL--DVEAHSFQPAIHNLVN 374
>BF177625
Length = 284
Score = 26.9 bits (58), Expect = 7.4
Identities = 18/83 (21%), Positives = 40/83 (47%), Gaps = 1/83 (1%)
Frame = +1
Query: 134 YGALLNCYCKELMVNKALAHFDKMNELGYV-TFLSFNNLIGMYMRLGQPLKVHELVHDMK 192
Y +LNC + L V++ FD+M + G+ +++N ++ ++ + KV L K
Sbjct: 16 YSCVLNCCSQALPVDELSRLFDEMLQRGFAPNTITYNVMLDVFGKAKLFRKVRRLYFMAK 195
Query: 193 ERNIPMSAFAYHAWMNSYAALND 215
++ + + Y+ + +Y D
Sbjct: 196 KQGL-VDVITYNTIIAAYGKNKD 261
>AU089121
Length = 584
Score = 26.6 bits (57), Expect = 9.7
Identities = 12/21 (57%), Positives = 16/21 (76%), Gaps = 2/21 (9%)
Frame = +2
Query: 266 VKPRKREAY--HCLLSLYAGT 284
+K RKR A+ HC LS+Y+GT
Sbjct: 164 IKQRKRRAHITHCDLSIYSGT 226
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,840,126
Number of Sequences: 28460
Number of extensions: 96856
Number of successful extensions: 441
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 432
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 438
length of query: 483
length of database: 4,897,600
effective HSP length: 94
effective length of query: 389
effective length of database: 2,222,360
effective search space: 864498040
effective search space used: 864498040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0239.15