BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0231b.3
(490 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV414637 133 6e-32
TC19141 similar to UP|PUR1_SOYBN (P52418) Amidophosphoribosyltra... 75 2e-14
TC18688 weakly similar to UP|Q9LP77 (Q9LP77) T1N15.9, partial (13%) 35 0.028
TC9459 similar to AAQ23982 (AAQ23982) Transcription factor Rap21... 34 0.062
TC10958 similar to PIR|T47938|T47938 RING finger protein - Arabi... 33 0.081
BP044109 33 0.14
TC12189 similar to UP|Q9FKV6 (Q9FKV6) Eukaryotic translation ini... 33 0.14
BP032533 33 0.14
TC14964 weakly similar to UP|SOX_ARATH (Q9SJA7) Potential sarcos... 33 0.14
TC9423 similar to UP|Q8S8Z8 (Q8S8Z8) Syringolide-induced protein... 32 0.18
TC17661 weakly similar to UP|Q09085 (Q09085) Hydroxyproline-rich... 31 0.40
TC9527 similar to GB|AAB04607.1|1448917|ATHTHSY threonine syntha... 31 0.40
TC20232 31 0.52
TC14338 similar to UP|Q9MAH5 (Q9MAH5) F12M16.16, partial (33%) 31 0.52
TC8179 weakly similar to GB|AAC96325.1|4028592|AF104902 ZIC2 pro... 31 0.52
TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor k... 30 0.68
TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%) 30 0.68
TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precu... 30 0.89
BP028499 30 0.89
TC12082 weakly similar to UP|Q40385 (Q40385) Pistil extensin-lik... 30 1.2
>AV414637
Length = 227
Score = 133 bits (335), Expect = 6e-32
Identities = 64/75 (85%), Positives = 72/75 (95%)
Frame = +3
Query: 92 EASRLCYLALHALQHRGQEGAGIVTVHNNKIFQSVTGVGLVADVFTEAKLDQLPGTLAIG 151
EASRLCYLALHALQHRGQEGAGIVTV+++ + QS+TGVGLV+DVF E+KLDQLPG+LAIG
Sbjct: 3 EASRLCYLALHALQHRGQEGAGIVTVNDSNVLQSITGVGLVSDVFNESKLDQLPGSLAIG 182
Query: 152 HVRYSTAGQSMLKNV 166
HVRYSTAGQSMLKNV
Sbjct: 183 HVRYSTAGQSMLKNV 227
>TC19141 similar to UP|PUR1_SOYBN (P52418) Amidophosphoribosyltransferase,
chloroplast precursor (Glutamine
phosphoribosylpyrophosphate amidotransferase) (ATASE)
(GPAT) , partial (8%)
Length = 488
Score = 75.1 bits (183), Expect = 2e-14
Identities = 39/65 (60%), Positives = 50/65 (76%), Gaps = 1/65 (1%)
Frame = -2
Query: 186 LVNYRSLREKLEENGSIF-NTSSDTEVVLHLIATSKQRPFILRIVDACEKLKGAYSFVFV 244
+ NY +L+ K+E+N SI NT+SDTEVVLHLIATSK R +LR +DAC KL+G+YS + V
Sbjct: 247 VANYGTLKAKVEDNESILINTTSDTEVVLHLIATSKCRSLLLRAIDACHKLQGSYSLMAV 68
Query: 245 TEDKL 249
E KL
Sbjct: 67 AE*KL 53
>TC18688 weakly similar to UP|Q9LP77 (Q9LP77) T1N15.9, partial (13%)
Length = 514
Score = 35.0 bits (79), Expect = 0.028
Identities = 27/85 (31%), Positives = 32/85 (36%), Gaps = 25/85 (29%)
Frame = +3
Query: 7 STLSSSSSSFSKSKP-------------------------FTNTPCFPGNKTLHNPTSFP 41
S SSSSSS S S P F+ TP P T T+ P
Sbjct: 63 SLSSSSSSSSSSSSPLSLNQISPPNAPPS*LSAPPSPEERFSGTPPPPPPATGSASTATP 242
Query: 42 NPTTLSHAHKPPYPSPCKNHNTTFP 66
P T S + PP PSP +H + P
Sbjct: 243 TPPTSSRSASPPSPSPANSHMASSP 317
>TC9459 similar to AAQ23982 (AAQ23982) Transcription factor Rap212,
partial (12%)
Length = 434
Score = 33.9 bits (76), Expect = 0.062
Identities = 20/57 (35%), Positives = 29/57 (50%)
Frame = +3
Query: 13 SSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSPCKNHNTTFPEPL 69
++S ++S N+ C P + ++P+S P T S A PP P P TT P PL
Sbjct: 6 NASKNRSNSSPNSTCQPSPPSKNSPSSSPPSPTSSPAPPPPSPPP---FQTTKPPPL 167
>TC10958 similar to PIR|T47938|T47938 RING finger protein - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (38%)
Length = 779
Score = 33.5 bits (75), Expect = 0.081
Identities = 20/61 (32%), Positives = 26/61 (41%), Gaps = 5/61 (8%)
Frame = +1
Query: 13 SSSFSKSKPFTNTPCFPGNK-----TLHNPTSFPNPTTLSHAHKPPYPSPCKNHNTTFPE 67
SS+FS S P + P P + T PT+ P P S PP SP + + P
Sbjct: 130 SSTFSSSSPLSAPPSPPSSASSTSPTSSTPTTSPPPHRTSPTRPPPLHSPQSSSGNSSPS 309
Query: 68 P 68
P
Sbjct: 310 P 312
>BP044109
Length = 497
Score = 32.7 bits (73), Expect = 0.14
Identities = 21/53 (39%), Positives = 27/53 (50%)
Frame = +2
Query: 5 NFSTLSSSSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSP 57
N +T S +S++ SKS P T TP P +PTS P P + A KP P
Sbjct: 140 NKTTPSHASNATSKSTPRTQTPTTPP----PSPTSSPKPPPSTFATKPSTSPP 286
Score = 28.5 bits (62), Expect = 2.6
Identities = 20/70 (28%), Positives = 28/70 (39%), Gaps = 3/70 (4%)
Frame = +2
Query: 15 SFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAH---KPPYPSPCKNHNTTFPEPLCN 71
S ++P P +L + +S T+LS +H PP P P N N T P N
Sbjct: 2 SLQSTRPEQQQPPHTATTSLSHSSS----TSLSSSHFFPSPPPPPPPPNKNKTTPSHASN 169
Query: 72 DDEDQKPREE 81
PR +
Sbjct: 170ATSKSTPRTQ 199
>TC12189 similar to UP|Q9FKV6 (Q9FKV6) Eukaryotic translation initiation
factor 3 subunit 7, partial (30%)
Length = 658
Score = 32.7 bits (73), Expect = 0.14
Identities = 18/41 (43%), Positives = 23/41 (55%)
Frame = +1
Query: 17 SKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSP 57
SKS F +TP G+ T PT PT+LS + PP P+P
Sbjct: 40 SKSAQFLSTPTVGGHWTTQPPT----PTSLSTSPSPPSPAP 150
>BP032533
Length = 441
Score = 32.7 bits (73), Expect = 0.14
Identities = 20/62 (32%), Positives = 28/62 (44%), Gaps = 9/62 (14%)
Frame = +3
Query: 7 STLSSSSSSFSKSKPFTNTPCFPG---------NKTLHNPTSFPNPTTLSHAHKPPYPSP 57
S S++S+S S S PF+++P P N H+PTS TL PP P
Sbjct: 159 SNSSATSASHSSSSPFSSSPSSPSHTNHRTHGLNLHRHSPTSSQKQKTLPSTSTPPSLKP 338
Query: 58 CK 59
+
Sbjct: 339 VR 344
>TC14964 weakly similar to UP|SOX_ARATH (Q9SJA7) Potential sarcosine oxidase
, partial (33%)
Length = 612
Score = 32.7 bits (73), Expect = 0.14
Identities = 16/62 (25%), Positives = 29/62 (45%)
Frame = +1
Query: 7 STLSSSSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSPCKNHNTTFP 66
S ++S+++S S ++P + T +PT+ + + PP PSPC + T
Sbjct: 337 SRIASATTSPMNSSTAASSPRSSPENSTSRTTGLSSPTSTAVSSSPPKPSPCSRRSPTNS 516
Query: 67 EP 68
P
Sbjct: 517 AP 522
>TC9423 similar to UP|Q8S8Z8 (Q8S8Z8) Syringolide-induced protein B13-1-9,
partial (53%)
Length = 969
Score = 32.3 bits (72), Expect = 0.18
Identities = 21/71 (29%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Frame = +2
Query: 2 AASNFSTLSSSSSSFSKSKPFTNTPCFPGNKTL---HNPTSFPNPTTLSHAHKPPYPSPC 58
AA + SS+SS S+ +++P + + T H P+S +PT SH+ P P+
Sbjct: 143 AAETAAAASSASSGRLSSQSSSSSPSYSSSSTSLSNHAPSSSMSPTPNSHSSTTPPPTTT 322
Query: 59 KNHNTTFPEPL 69
T+F L
Sbjct: 323 TATTTSFATTL 355
>TC17661 weakly similar to UP|Q09085 (Q09085) Hydroxyproline-rich
glycoprotein (HRGP) (Fragment), partial (8%)
Length = 417
Score = 31.2 bits (69), Expect = 0.40
Identities = 18/49 (36%), Positives = 27/49 (54%)
Frame = +1
Query: 20 KPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSPCKNHNTTFPEP 68
+P + P P +LH+P+ P+PT H H PP +P +H+T F P
Sbjct: 28 QPPPSPPPPPPCHSLHSPS--PSPTPPPHPHGPPRINPL-HHSTLFNPP 165
>TC9527 similar to GB|AAB04607.1|1448917|ATHTHSY threonine synthase
{Arabidopsis thaliana;} , partial (23%)
Length = 544
Score = 31.2 bits (69), Expect = 0.40
Identities = 22/60 (36%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -1
Query: 12 SSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSH-AHKPPYPSPCKNHNTTFPEPLC 70
S SFS S + P + G T S NP T SH + PP P P +HN++ P C
Sbjct: 205 SPRSFSASSVPSKPPQYAGK*T-----SQSNPPTPSHPSAPPPSPPPQSHHNSSAHAPPC 41
>TC20232
Length = 601
Score = 30.8 bits (68), Expect = 0.52
Identities = 16/38 (42%), Positives = 18/38 (47%), Gaps = 8/38 (21%)
Frame = -2
Query: 37 PTSFPNPTT--------LSHAHKPPYPSPCKNHNTTFP 66
P SFPNPT LS H+PP P P + T P
Sbjct: 132 PPSFPNPTPP*PPTASPLSRPHRPPPPPPPSTSSLTSP 19
>TC14338 similar to UP|Q9MAH5 (Q9MAH5) F12M16.16, partial (33%)
Length = 1751
Score = 30.8 bits (68), Expect = 0.52
Identities = 23/62 (37%), Positives = 29/62 (46%), Gaps = 4/62 (6%)
Frame = +3
Query: 11 SSSSSFSKSKPFTNTPCFPGNKTLHNPTSFP-NPTTLSHAH---KPPYPSPCKNHNTTFP 66
SS +SF K +P T P L P P +P +LS A PPYPSP ++ P
Sbjct: 36 SSLASFPKPEP*T*LPSSLAPSPLQPPPPLPPSPPSLSSAATVPSPPYPSPPAATSSPPP 215
Query: 67 EP 68
P
Sbjct: 216AP 221
>TC8179 weakly similar to GB|AAC96325.1|4028592|AF104902 ZIC2 protein {Homo
sapiens;} , partial (4%)
Length = 587
Score = 30.8 bits (68), Expect = 0.52
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
Frame = -3
Query: 11 SSSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTT---LSHAHKPPYPSP 57
SSSSS S + +++ P + + P S P+ T+ LS +HKP P+P
Sbjct: 438 SSSSSSSSASTASHSGYSPSSDSKTEPASTPSATSSKALSSSHKPDPPTP 289
>TC19569 similar to UP|AAR11301 (AAR11301) Lectin-like receptor kinase 1;1,
partial (16%)
Length = 486
Score = 30.4 bits (67), Expect = 0.68
Identities = 23/76 (30%), Positives = 32/76 (41%), Gaps = 5/76 (6%)
Frame = +1
Query: 8 TLSSSSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSPCKNHNTTFP- 66
T SSSSSS+S P P + H+P++ P TT PP P+ + T P
Sbjct: 43 TSSSSSSSYSPPPP-------PSQQPTHSPSTSPTSTTT----PPPSPTKVTENPPTAPS 189
Query: 67 ----EPLCNDDEDQKP 78
P + E+ P
Sbjct: 190 TSTKSPTSSASEEPSP 237
>TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%)
Length = 1036
Score = 30.4 bits (67), Expect = 0.68
Identities = 22/67 (32%), Positives = 29/67 (42%)
Frame = +3
Query: 2 AASNFSTLSSSSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPYPSPCKNH 61
+A + S SSSSSS S S +P P+ P + + PP PSP
Sbjct: 189 SAPSASG*SSSSSSSSSSSAVLASPSTSSTAPTTPPSPSPPSNSPTSTSPPPQPSP---P 359
Query: 62 NTTFPEP 68
N+T P P
Sbjct: 360 NSTSPSP 380
Score = 29.6 bits (65), Expect = 1.2
Identities = 22/71 (30%), Positives = 33/71 (45%), Gaps = 9/71 (12%)
Frame = +3
Query: 7 STLSSSSSSFSKSKPFTNT--------PCFPGNK-TLHNPTSFPNPTTLSHAHKPPYPSP 57
S+ SSSSSS + P T++ P P N T +P P+P + P P+
Sbjct: 219 SSSSSSSSSAVLASPSTSSTAPTTPPSPSPPSNSPTSTSPPPQPSPPNSTSPSPPQTPTR 398
Query: 58 CKNHNTTFPEP 68
+H+ T+P P
Sbjct: 399 KTSHSPTYPLP 431
>TC14645 similar to UP|Q41122 (Q41122) Proline-rich protein precursor,
partial (52%)
Length = 1060
Score = 30.0 bits (66), Expect = 0.89
Identities = 17/42 (40%), Positives = 19/42 (44%), Gaps = 2/42 (4%)
Frame = +2
Query: 29 PGNKTLHNPTSFP--NPTTLSHAHKPPYPSPCKNHNTTFPEP 68
P +H PTS P PT H H PP P+P TT P
Sbjct: 326 PIKPPVHPPTSVPVKPPTGHHHHHHPPSPAPAPALYTTITLP 451
>BP028499
Length = 491
Score = 30.0 bits (66), Expect = 0.89
Identities = 23/79 (29%), Positives = 34/79 (42%), Gaps = 17/79 (21%)
Frame = +3
Query: 7 STLSSSSSSFSKSKPFTNTPCFPGNKTLHNPTSFPNPTTLSHAHKPPY-------PSPCK 59
S++ S ++S + S + P P TL +P S P+ + S +H P PSP
Sbjct: 141 SSI*SHNASTAHSTSSASDPSVPPGATLPHPPSSPSTISPSISHPSPITITTTLPPSPSP 320
Query: 60 N----------HNTTFPEP 68
N H TT P+P
Sbjct: 321 NAPSSSSPHHHHRTTKPKP 377
>TC12082 weakly similar to UP|Q40385 (Q40385) Pistil extensin-like protein,
partial (11%)
Length = 308
Score = 29.6 bits (65), Expect = 1.2
Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 4/47 (8%)
Frame = +1
Query: 26 PCFPGNKTLHNPTS----FPNPTTLSHAHKPPYPSPCKNHNTTFPEP 68
P N + +PTS P PT PP P P +H++T P P
Sbjct: 79 PWILANLRIRSPTSPWDPLPTPTPTPTPPSPPPPWPLDSHHSTPPPP 219
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.136 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,546,906
Number of Sequences: 28460
Number of extensions: 146898
Number of successful extensions: 1472
Number of sequences better than 10.0: 114
Number of HSP's better than 10.0 without gapping: 1335
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1431
length of query: 490
length of database: 4,897,600
effective HSP length: 94
effective length of query: 396
effective length of database: 2,222,360
effective search space: 880054560
effective search space used: 880054560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0231b.3