BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0220.7
(720 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV427921 82 4e-16
BP063694 80 1e-15
AV766088 71 5e-13
AU088980 71 7e-13
TC8302 70 9e-13
TC9039 60 1e-09
BP033602 59 3e-09
BP052632 49 4e-06
BP058111 43 2e-04
TC8301 42 3e-04
AV764934 41 8e-04
BP061842 40 0.001
BP063902 40 0.001
BP078157 40 0.002
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 38 0.006
BP066067 36 0.019
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 36 0.025
AV420911 35 0.042
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 35 0.042
BP081717 34 0.071
>AV427921
Length = 284
Score = 81.6 bits (200), Expect = 4e-16
Identities = 33/87 (37%), Positives = 56/87 (63%)
Frame = +1
Query: 573 TPEQNGVAERMNRTLNERARCMRIQSGLPKMFWPDAINTAAYLINRGPSVPLDYQLPEEV 632
TP+QNGV+ER NRT+ R + SGLPK F P+A+ + +++NR P++ + +PEE
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 633 WYGKEVSLSHLKVFGCVSYVLIDSDKR 659
W G++ ++ H ++ C++Y + KR
Sbjct: 202 WSGRQPAVDHFRISRCLAYAHVPDQKR 282
>BP063694
Length = 511
Score = 79.7 bits (195), Expect = 1e-15
Identities = 47/156 (30%), Positives = 82/156 (52%), Gaps = 5/156 (3%)
Frame = -2
Query: 384 KGNLVVARGKKRGSLYMVAEEDMIAVTEAIN----SSSIWHQRLGHMSEKGMKIMASKGK 439
K V+ R + LY++ ++ +T + S +WH RLGH++ +K + G
Sbjct: 474 KTGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIKQLHKHGC 295
Query: 440 MSNLKHVDLGVC-EHCILGKQRKVSFSKAGRKSKSEKLELVHTDVWGPAPVKSLGGSRYY 498
+ + +C C + K +++ F +++ S L+L+H D+ GP+PV S+ G Y+
Sbjct: 294 LDVSSILPKPICCTSCQMAKSKRLVFHDNNKRA-SAVLDLIHCDLRGPSPVASIDGFSYF 118
Query: 499 VTFIDDSTRKVWVYFLKSKSDVFSVFKKWKTEVENQ 534
V F+DD +R W Y LK KSD V ++K +EN+
Sbjct: 117 VIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMENR 10
>AV766088
Length = 501
Score = 71.2 bits (173), Expect = 5e-13
Identities = 37/107 (34%), Positives = 59/107 (54%)
Frame = -3
Query: 415 SSSIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKSE 474
+ S+WH RLGH S ++ + S +S VCE C+ GK ++ F + +
Sbjct: 316 AQSLWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMP 137
Query: 475 KLELVHTDVWGPAPVKSLGGSRYYVTFIDDSTRKVWVYFLKSKSDVF 521
+++H+D+W +PV S G R+YV F+DD T +W + L +KS VF
Sbjct: 136 -FDILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>AU088980
Length = 360
Score = 70.9 bits (172), Expect = 7e-13
Identities = 41/109 (37%), Positives = 54/109 (48%)
Frame = +2
Query: 577 NGVAERMNRTLNERARCMRIQSGLPKMFWPDAINTAAYLINRGPSVPLDYQLPEEVWYGK 636
N + ER +R + R + S LPK W A+ AAYLIN PS L Q P +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 637 EVSLSHLKVFGCVSYVLIDSDKRDKLDPKAIKCFFIGYGSDMYGYRFWD 685
+L LKVFG + Y + KLDP+A KC F+G+ + GY D
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMD 328
>TC8302
Length = 494
Score = 70.5 bits (171), Expect = 9e-13
Identities = 47/161 (29%), Positives = 76/161 (47%)
Frame = +3
Query: 102 RRLFNLRMGEGNSVTEHINSFNTIISQLSSVKITFDNELMVLSLLQSLPDSWAATVTAVS 161
+RL+ LRM E S+++HI+ NT+ +QLS+ T LL+SLPDS+ V +
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 162 NSARDNKLKFDDIRDLILSEDIRRKDSGESSNTFGSALNTESRGRGSQKSHNQSQGRGRS 221
N+ N L + +++L ED +R N E R S+ + RGRS
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR-------------*NKEDR*DSSKPMEALTMTRGRS 323
Query: 222 KSRGRSQTRVRNDITCWNCDRKGHFTNQCKAPRKKKNYQKR 262
KSR + ++ C++C ++ H C +K + R
Sbjct: 324 KSRKKI------NLKCYHCGQR*HLKKDCWFNKKSSDVTPR 428
>TC9039
Length = 1218
Score = 60.1 bits (144), Expect = 1e-09
Identities = 51/222 (22%), Positives = 101/222 (44%), Gaps = 6/222 (2%)
Frame = +2
Query: 35 PLTGKKPNDMKQ----EDWDLLDRQALGVIRLTLSKNVAFNIVNE-KTTADLMKALSNMY 89
PL +K + +Q E WD +R +L +I+ + + I E K D + + +
Sbjct: 251 PLLLRKSSTSEQRKDYEKWDRSNRMSLMIIKRGIPEVFRGTISEEIKGAKDFLAEIEKRF 430
Query: 90 EKPFAANKVHLIRRLFNLR-MGEGNSVTEHINSFNTIISQLSSVKITFDNELMVLSLLQS 148
K A L++ L +++ G+GN + E+I + I S+L ++K+ ++L++ +L S
Sbjct: 431 AKSDKAETSTLLQNLISMKYQGKGN-IREYIMGMSNIASKLKALKLELSDDLLIHLVLLS 607
Query: 149 LPDSWAATVTAVSNSARDNKLKFDDIRDLILSEDIRRKDSGESSNTFGSALNTESRGRGS 208
LP ++ +S + K +++ + E+ R K + S F S S+ +G
Sbjct: 608 LPAQFSQF--KISYNCPKEKWSLNELISFCVQEEERLKQERKESAHFVST----SKDKGK 769
Query: 209 QKSHNQSQGRGRSKSRGRSQTRVRNDITCWNCDRKGHFTNQC 250
+K + + + Q + D TC+ C+ GH +C
Sbjct: 770 RKKTVEPKNEAADAPAPKKQ---KEDDTCYFCNVSGHMKKKC 886
Score = 48.9 bits (115), Expect = 3e-06
Identities = 26/86 (30%), Positives = 45/86 (52%), Gaps = 2/86 (2%)
Frame = +3
Query: 319 VYLADGKPLDIVGIGDIDIRSSNGTLWTLHNVRHVPGIKRNLISIGQLDDEGYHTTFGGG 378
+Y+ DGK +++ IG + G L + VP +RNLIS+ LD GY +FG
Sbjct: 960 IYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLDKSGYSCSFGNS 1139
Query: 379 AWKVTKGNLVVARGKKRG--SLYMVA 402
+++ + +V G G +LY+++
Sbjct: 1140 KAELSFNSNIVGTGSLIGYDNLYLLS 1217
>BP033602
Length = 533
Score = 58.9 bits (141), Expect = 3e-09
Identities = 31/101 (30%), Positives = 54/101 (52%), Gaps = 1/101 (0%)
Frame = +3
Query: 407 IAVTEAINSSSIWHQRLGHMSEKGMK-IMASKGKMSNLKHVDLGVCEHCILGKQRKVSFS 465
++ T + +WH RLGH + + ++ + K N ++ CE C+L K ++ +
Sbjct: 243 MSCTSVRDQIMLWHNRLGHPNFQYLRHLFPDLFKNVNCSSLE---CESCVLAKNQRAPYY 413
Query: 466 KAGRKSKSEKLELVHTDVWGPAPVKSLGGSRYYVTFIDDST 506
+ S L+H+DVWGP+ + + G R++VTFIDD T
Sbjct: 414 SQPYHA-SRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>BP052632
Length = 489
Score = 48.5 bits (114), Expect = 4e-06
Identities = 23/73 (31%), Positives = 40/73 (54%)
Frame = -3
Query: 629 PEEVWYGKEVSLSHLKVFGCVSYVLIDSDKRDKLDPKAIKCFFIGYGSDMYGYRFWDEQN 688
P E+W G+ ++S+ FGC ++L +D K D K K +GY S+ Y ++ +
Sbjct: 481 PYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNSRT 302
Query: 689 RKIIRSINVTFNE 701
+ + SINV F++
Sbjct: 301 QVVEESINVKFDD 263
>BP058111
Length = 570
Score = 42.7 bits (99), Expect = 2e-04
Identities = 23/65 (35%), Positives = 35/65 (53%)
Frame = -1
Query: 417 SIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKSEKL 476
++WH RLGH S K + I+ K + C+ C + KQ+K+SF + S SE
Sbjct: 189 NLWHLRLGHTSSKKLAILQKNFPFITCKKI-TSPCDTCHMAKQKKLSFPNSVTLS-SEIF 16
Query: 477 ELVHT 481
+L+HT
Sbjct: 15 DLIHT 1
>TC8301
Length = 1243
Score = 42.4 bits (98), Expect = 3e-04
Identities = 23/45 (51%), Positives = 30/45 (66%), Gaps = 1/45 (2%)
Frame = +3
Query: 100 LIRRLFNLRMGEGNSVTEHINSFNTIISQLSSVKITF-DNELMVL 143
L RRL LRM E S+ +HIN+ NT+ +QLS+ T +NELM L
Sbjct: 789 LKRRL*TLRMSESTSMPDHINNLNTMFAQLSASDFTIGENELMTL 923
>AV764934
Length = 406
Score = 40.8 bits (94), Expect = 8e-04
Identities = 24/75 (32%), Positives = 36/75 (48%)
Frame = -2
Query: 417 SIWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKSEKL 476
S WH RLGH +K + S +S + C LGK ++ + +
Sbjct: 219 STWHSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHRLPSQMS--TTTYLPF 46
Query: 477 ELVHTDVWGPAPVKS 491
EL+++D+WGPAPV S
Sbjct: 45 ELIYSDLWGPAPVCS 1
>BP061842
Length = 318
Score = 40.4 bits (93), Expect = 0.001
Identities = 16/28 (57%), Positives = 21/28 (74%)
Frame = -3
Query: 476 LELVHTDVWGPAPVKSLGGSRYYVTFID 503
LELV D+WGPAP S G +YY++F+D
Sbjct: 88 LELVPCDLWGPAPCVSTNGFKYYLSFVD 5
>BP063902
Length = 494
Score = 40.0 bits (92), Expect = 0.001
Identities = 23/84 (27%), Positives = 37/84 (43%)
Frame = -3
Query: 384 KGNLVVARGKKRGSLYMVAEEDMIAVTEAINSSSIWHQRLGHMSEKGMKIMASKGKMSNL 443
K + + R G LY V A +SS+WH RLGH + + + + +
Sbjct: 273 KTGMPLLRCNSLGDLYPVTRSSPFAGL----ASSVWHHRLGHPASSALNHLRNNKLIFCE 106
Query: 444 KHVDLGVCEHCILGKQRKVSFSKA 467
VC+ C+LGK ++ FS +
Sbjct: 105 PSRSSSVCDSCVLGKHVRLPFSSS 34
>BP078157
Length = 395
Score = 39.7 bits (91), Expect = 0.002
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = -2
Query: 418 IWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSF 464
+WH RLGH+S+K +K++++ + L C C L K R++SF
Sbjct: 148 LWHSRLGHLSDKVLKVVSNLVPFTVLPDFHSHNCNVCPLSKMRRLSF 8
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 37.7 bits (86), Expect = 0.006
Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
Frame = +2
Query: 166 DNKLKFDDIRDLILSEDIR-RKDSGESSNTFGSALNTESRGRGSQKSHNQSQGRGRSKSR 224
D+K D+ D + D+ R + + + GS+ + + R ++ ++ R +SR
Sbjct: 203 DDKKAMDEAIDAMNGMDLDGRTITVDKAQPQGSSRDRDDGDRYRERGRDRDDRGDRDRSR 382
Query: 225 GRSQTRVRNDITCWNCDRKGHFTNQC 250
G +R N C+ C + GHF +C
Sbjct: 383 GYGGSRGSNGGECFKCGKPGHFAREC 460
>BP066067
Length = 505
Score = 36.2 bits (82), Expect = 0.019
Identities = 24/75 (32%), Positives = 39/75 (52%), Gaps = 3/75 (4%)
Frame = -2
Query: 290 SWVIDSGASFH-TIPSKELLSNYICGKFGKVYLADGKPLDIVGIGDIDIRSS--NGTLWT 346
SW DSGAS H T + L+ + +VY+ G+ L I+ IG S + T ++
Sbjct: 300 SW*ADSGASHHVTNDPQHLMESITMPGQEQVYVCSGQGLPILSIGFTSFSSPLISNTKFS 121
Query: 347 LHNVRHVPGIKRNLI 361
L + H+P I +N++
Sbjct: 120 LKDFFHLPTITKNIV 76
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 35.8 bits (81), Expect = 0.025
Identities = 26/83 (31%), Positives = 39/83 (46%)
Frame = +1
Query: 169 LKFDDIRDLILSEDIRRKDSGESSNTFGSALNTESRGRGSQKSHNQSQGRGRSKSRGRSQ 228
+ FDD RD ++D R+ G+ N + L+ SRG G +G GRS G
Sbjct: 211 IDFDDRRD---AQDAIRELDGK--NGWRVELSHNSRGGGG--GGGGGRGGGRSGGGGGG- 366
Query: 229 TRVRNDITCWNCDRKGHFTNQCK 251
+D+ C+ C GHF +C+
Sbjct: 367 ----SDMKCYECGEPGHFARECR 423
>AV420911
Length = 418
Score = 35.0 bits (79), Expect = 0.042
Identities = 25/83 (30%), Positives = 36/83 (43%)
Frame = +2
Query: 169 LKFDDIRDLILSEDIRRKDSGESSNTFGSALNTESRGRGSQKSHNQSQGRGRSKSRGRSQ 228
L+FDD RD + + I D N + L+ S+G G GRG + RG
Sbjct: 227 LEFDDKRDAL--DAIHALDG---KNGWRVELSHNSKGGGG--------GRGGGRGRGGE- 364
Query: 229 TRVRNDITCWNCDRKGHFTNQCK 251
D+ C+ C GHF +C+
Sbjct: 365 -----DLKCYECGEPGHFARECR 418
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 35.0 bits (79), Expect = 0.042
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = +1
Query: 211 SHNQSQGRGRSKSRGRSQTRVRNDITCWNCDRKGHFTNQCKA 252
SHN G G RG + R D+ C+ C GHF +C++
Sbjct: 97 SHNSKGGGG---GRGGGRGRGGEDLKCYECGEPGHFARECRS 213
>BP081717
Length = 291
Score = 34.3 bits (77), Expect = 0.071
Identities = 19/56 (33%), Positives = 32/56 (56%)
Frame = -1
Query: 418 IWHQRLGHMSEKGMKIMASKGKMSNLKHVDLGVCEHCILGKQRKVSFSKAGRKSKS 473
+WH RLGH S K ++ ++ L H ++ +C+ C + KQ K+ F + KSK+
Sbjct: 168 MWHFRLGHPSSKVLQHISKVFPYVKL-HSNI-ICDFCHMAKQTKLPFPISDTKSKA 7
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,803,920
Number of Sequences: 28460
Number of extensions: 155415
Number of successful extensions: 871
Number of sequences better than 10.0: 60
Number of HSP's better than 10.0 without gapping: 825
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 844
length of query: 720
length of database: 4,897,600
effective HSP length: 97
effective length of query: 623
effective length of database: 2,136,980
effective search space: 1331338540
effective search space used: 1331338540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0220.7