BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0220.1
(751 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13225 122 2e-28
TC16379 similar to UP|O22265 (O22265) Expressed protein (At2g474... 35 0.057
BP051993 35 0.057
TC15308 similar to UP|Q41725 (Q41725) TED3, partial (16%) 34 0.075
AV409313 32 0.37
BP039702 31 0.83
TC14333 31 0.83
TC12711 similar to UP|Q84Y18 (Q84Y18) CAX-interacting protein 4,... 30 1.1
TC17622 weakly similar to UP|P93837 (P93837) Amp-binding protein... 30 1.8
TC20242 similar to UP|Q9LXK4 (Q9LXK4) Guanine nucleotide-exchang... 30 1.8
AV419448 30 1.8
AV421977 30 1.8
TC14326 weakly similar to UP|EXLP_TOBAC (Q03211) Pistil-specific... 29 2.4
TC8786 similar to UP|Q9ZTK7 (Q9ZTK7) CONSTANS-like protein 2, pa... 29 2.4
TC18118 29 2.4
TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%) 29 2.4
TC7801 similar to UP|Q8VX01 (Q8VX01) Casein kinase II alpha subu... 29 2.4
TC17096 similar to PIR|C53394|C53394 ribosomal protein L12.C pre... 29 3.2
BP044070 29 3.2
TC10607 29 3.2
>TC13225
Length = 513
Score = 122 bits (307), Expect = 2e-28
Identities = 64/162 (39%), Positives = 94/162 (57%), Gaps = 2/162 (1%)
Frame = +3
Query: 570 IKTDEEGNPIIEDGKFI--LDAVNSLIFTIAQHFVGDPSLIKDRFGDLLSNLKCKSLGDF 627
I T E+ + I+E K DAV +LI+TI HF+GDPS+ ++R L+NL C ++ D+
Sbjct: 3 IMTPEDKSTILEKKKENGEEDAVATLIYTIILHFIGDPSIFRERASSQLANLYCPTMSDY 182
Query: 628 RWYKDTFLTRVYTREDSQQAFWKEKFLAGLPKSFGDKVREKLRSQNPGGEIPYHTLSYGQ 687
RWYKDTF ++V RED AFWKE+F+AGLP+ KV + L N G + + +LS+GQ
Sbjct: 183 RWYKDTFFSKVTLREDGNSAFWKERFIAGLPRLMQSKVLDNLSLFNQGNPVNFGSLSFGQ 362
Query: 688 LIAIIQRVALKICQDDRIQQQLTKEKSQNRRDLGTFCEQFGI 729
L I ++ + +SQ R +FCE +G+
Sbjct: 363 LHNTIVHTGIQFVLTSNFKTNAKDMQSQKRS--SSFCEXYGV 482
>TC16379 similar to UP|O22265 (O22265) Expressed protein
(At2g47450/T30B22.25), partial (16%)
Length = 449
Score = 34.7 bits (78), Expect = 0.057
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +3
Query: 385 LLQKVVEQLQLLNQVIPETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNIEDGKVE 444
LL LQ P T +P A +NP S P+PP ++TS + T L +
Sbjct: 48 LLTSNSNHLQTPTSSFPTTANLSPSAAANPFTSPPSPPHSKTSRKPPMTLLKKMNPTAKS 227
Query: 445 SDS 447
++S
Sbjct: 228 NES 236
>BP051993
Length = 548
Score = 34.7 bits (78), Expect = 0.057
Identities = 29/107 (27%), Positives = 51/107 (47%)
Frame = -2
Query: 359 FKHFQISNRSRHSAQTLRAKNESDKELLQKVVEQLQLLNQVIPETPEPTPEAVSNPAVSA 418
+ FQ+ + + + T+ E +K L++ V E L+ +Q I E P +S+ A
Sbjct: 457 YVEFQLDDLAGFPSNTV----EEEKMLMEAVTESLK--DQEIKNPSEEQPPCLSSRA--- 305
Query: 419 PAPPKTRTSTRNTSTSLNNIEDGKVESDSEDQSVKSIPVNTVIHNSR 465
APP + T + + S N+ E SDS S+K+ N + H ++
Sbjct: 304 -APPPSTFDTSSVTESGNSTESASACSDS--SSLKNSSENDISHKTK 173
>TC15308 similar to UP|Q41725 (Q41725) TED3, partial (16%)
Length = 1103
Score = 34.3 bits (77), Expect = 0.075
Identities = 51/193 (26%), Positives = 71/193 (36%), Gaps = 41/193 (21%)
Frame = +3
Query: 359 FKHFQISNRSRHSAQTLRAKNESDKE-------LLQKVVEQLQLLNQVIPETPEPTPEAV 411
F HF+ +N AQ L AK ES + L Q + IP P P PE +
Sbjct: 114 FTHFRTNNVK--DAQLLLAKAESPAQAPGPASALAPAPAPSAQAIGSTIPSGPAPEPEFL 287
Query: 412 ----------SNPAVSAPAPPKTRTSTRNTSTSLNNIEDGKVESDSEDQSVKSIPVNTVI 461
++ + SA P TST N E+ + D +DQS K+ T
Sbjct: 288 DMGEGYGLYGTDSSSSAQYSPTKETSTTT------NFENELLNEDFKDQSYKTGYPQT-- 443
Query: 462 HNSRNQWRKE--TKLY----YPRATAPDLLLEESSNFKSFSANNVYE------------- 502
N R+ + E TK Y Y L ++N ++ NN YE
Sbjct: 444 -NFRSNYNNEVYTKNYNTEGYRSNDNNGKELYYNNNNNNYGNNNGYERKREGMSDTRFME 620
Query: 503 -----WNIDGENE 510
+N+D ENE
Sbjct: 621 NGKYHYNVDTENE 659
>AV409313
Length = 352
Score = 32.0 bits (71), Expect = 0.37
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = -2
Query: 335 KVKTEPSSSSDLDIPKGCQLEKPLF 359
++K EPS SSD++ PKGC L LF
Sbjct: 330 RLK*EPSLSSDINKPKGCNLSASLF 256
>BP039702
Length = 553
Score = 30.8 bits (68), Expect = 0.83
Identities = 26/73 (35%), Positives = 32/73 (43%), Gaps = 5/73 (6%)
Frame = -2
Query: 402 ETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNIEDGKVESDSEDQS--VKS---IP 456
ETPEP P ++P APAPP S DS D+S V S +P
Sbjct: 468 ETPEPPPAPSASPL--APAPP*GSQSP---------------SPDSRDESPPVASGYVVP 340
Query: 457 VNTVIHNSRNQWR 469
TV H +R +WR
Sbjct: 339 QTTVSHCTRRRWR 301
>TC14333
Length = 602
Score = 30.8 bits (68), Expect = 0.83
Identities = 20/89 (22%), Positives = 33/89 (36%)
Frame = +1
Query: 255 WQHKDGKETLSNLPPKTPFLLKGAQSTPVLASPFKTREEEGEVTSKDIKDLMEQANYTNK 314
W H G T+S +PP T + +G P + F+ ++ + + Q Y N
Sbjct: 34 WVHGPGSRTMSAVPPSTYYSFQGQNQQP---AGFRQNQQPSQ------QQHFGQLGYPNF 186
Query: 315 YLQTLGESQSHDDSSRKGKGKVKTEPSSS 343
Y G S H ++ + S S
Sbjct: 187 YHSQTGMSLEHQQQQQQQNARESLAGSQS 273
>TC12711 similar to UP|Q84Y18 (Q84Y18) CAX-interacting protein 4, partial
(5%)
Length = 500
Score = 30.4 bits (67), Expect = 1.1
Identities = 18/56 (32%), Positives = 32/56 (57%)
Frame = +2
Query: 292 EEEGEVTSKDIKDLMEQANYTNKYLQTLGESQSHDDSSRKGKGKVKTEPSSSSDLD 347
+++ E T + K E+ ++ ++ +S++ DDSS + K K + E SSSSD D
Sbjct: 77 DDDDEYTRRHRKSRKEKRRRRSR--RSDSDSETSDDSSTRHKRKSQREKSSSSDTD 238
>TC17622 weakly similar to UP|P93837 (P93837) Amp-binding protein, partial
(24%)
Length = 461
Score = 29.6 bits (65), Expect = 1.8
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 2/45 (4%)
Frame = +3
Query: 403 TPEPTPEAVSNPAVSA--PAPPKTRTSTRNTSTSLNNIEDGKVES 445
+P P+P S P+ A PAP + ++T N S+S + GK+ S
Sbjct: 300 SPSPSPALSSTPSTPALTPAPSPSSSATPNPSSSSSIAPHGKLSS 434
>TC20242 similar to UP|Q9LXK4 (Q9LXK4) Guanine nucleotide-exchange-like
protein, partial (5%)
Length = 741
Score = 29.6 bits (65), Expect = 1.8
Identities = 27/87 (31%), Positives = 35/87 (40%), Gaps = 1/87 (1%)
Frame = +1
Query: 376 RAKNESDKELLQKVVEQLQLLNQVIPE-TPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTS 434
RA + KE K +LQ Q + T E + S P +AP+P S S
Sbjct: 334 RAFDSMLKECSGKKFPELQKAIQNFTDITKEASQRKQSEPNQAAPSPE---------SAS 486
Query: 435 LNNIEDGKVESDSEDQSVKSIPVNTVI 461
N EDG V DQS K+ N +
Sbjct: 487 ANETEDGAVTKPEADQSHKAYSGNITV 567
>AV419448
Length = 382
Score = 29.6 bits (65), Expect = 1.8
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 3/34 (8%)
Frame = +2
Query: 403 TPEPTPEAVSNPAVS---APAPPKTRTSTRNTST 433
T P+P +SNP+ S P PP T+T + +S+
Sbjct: 227 TSSPSPSPISNPSASLPPTPPPPPTKTPSSTSSS 328
>AV421977
Length = 535
Score = 29.6 bits (65), Expect = 1.8
Identities = 13/37 (35%), Positives = 20/37 (53%), Gaps = 3/37 (8%)
Frame = +2
Query: 400 IPETPEPTPEAVSNPAVSAPA---PPKTRTSTRNTST 433
+P P P P + ++P+ PA PP T+ S T+T
Sbjct: 233 LPPLPPPPPPSSTSPSTKTPAASPPPPTKASASTTAT 343
>TC14326 weakly similar to UP|EXLP_TOBAC (Q03211) Pistil-specific
extensin-like protein precursor (PELP), partial (11%)
Length = 630
Score = 29.3 bits (64), Expect = 2.4
Identities = 14/38 (36%), Positives = 18/38 (46%), Gaps = 4/38 (10%)
Frame = +2
Query: 395 LLNQVIPETPEPT----PEAVSNPAVSAPAPPKTRTST 428
++N IP P P P S PAV+ P PP + T
Sbjct: 113 VVNYAIPSVPAPPSPVKPPTPSTPAVTVPTPPLVKAPT 226
>TC8786 similar to UP|Q9ZTK7 (Q9ZTK7) CONSTANS-like protein 2, partial
(33%)
Length = 594
Score = 29.3 bits (64), Expect = 2.4
Identities = 16/56 (28%), Positives = 29/56 (51%)
Frame = +1
Query: 401 PETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTSLNNIEDGKVESDSEDQSVKSIP 456
P TP P P P++ + PP +ST NT + + N ++ K + + + +K +P
Sbjct: 319 PPTPSP-PATNVPPSLPSSTPPPPTSSTTNTCSQMQN-QNQKQKQQKKRKRLKLLP 480
>TC18118
Length = 617
Score = 29.3 bits (64), Expect = 2.4
Identities = 17/60 (28%), Positives = 26/60 (43%), Gaps = 4/60 (6%)
Frame = -3
Query: 3 SIKSYFLRTNCEKSGET----TLFLTDYDKANVIVPKTIQWSDITLPDKWSLEKAAPAQP 58
S+ +F+ NC +++L + + VI PKTI W T+P W P P
Sbjct: 243 SVAIHFISGNCSIL*HAPICQSIWLWNPGRREVINPKTIIWISFTIPHHWFPPWPLPGSP 64
>TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%)
Length = 1240
Score = 29.3 bits (64), Expect = 2.4
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +1
Query: 401 PETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTS 434
P+T TP S PA ++P P T + + +TST+
Sbjct: 148 PKTSTSTPTPASPPASTSPLTPPTTSPSSSTSTA 249
>TC7801 similar to UP|Q8VX01 (Q8VX01) Casein kinase II alpha subunit
precursor , partial (28%)
Length = 591
Score = 29.3 bits (64), Expect = 2.4
Identities = 14/42 (33%), Positives = 17/42 (40%)
Frame = +3
Query: 704 RIQQQLTKEKSQNRRDLGTFCEQFGIQGCPKKPKPRKHDPPP 745
R QQ S R F + P P+PR+H PPP
Sbjct: 12 RTAQQFHSPPSYLLRSPSFSLRHFAVSATPAPPQPRRHLPPP 137
>TC17096 similar to PIR|C53394|C53394 ribosomal protein L12.C precursor,
chloroplast - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (74%)
Length = 677
Score = 28.9 bits (63), Expect = 3.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 401 PETPEPTPEAVSNPAVSAPAPPKTRTSTRNTSTS 434
P+ P + + P+ SAP+PP R S + +TS
Sbjct: 72 PKPPSTSQHPSAAPSASAPSPPSPRKSRSSATTS 173
>BP044070
Length = 289
Score = 28.9 bits (63), Expect = 3.2
Identities = 15/28 (53%), Positives = 18/28 (63%), Gaps = 1/28 (3%)
Frame = +3
Query: 401 PETPEPTPEAVSNP-AVSAPAPPKTRTS 427
P T P+P + S P AVS+P PP TR S
Sbjct: 63 PATSAPSPPSNSPPTAVSSPPPPPTRPS 146
>TC10607
Length = 411
Score = 28.9 bits (63), Expect = 3.2
Identities = 17/47 (36%), Positives = 22/47 (46%), Gaps = 3/47 (6%)
Frame = +2
Query: 402 ETPEPTPEAVSNPA---VSAPAPPKTRTSTRNTSTSLNNIEDGKVES 445
++P P ++SNPA SAPAPP S + S S ES
Sbjct: 242 KSPPTKPSSMSNPAWSSASAPAPPPNTPSPESVSFSARGSSPTSSES 382
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.131 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,700,075
Number of Sequences: 28460
Number of extensions: 171616
Number of successful extensions: 1228
Number of sequences better than 10.0: 69
Number of HSP's better than 10.0 without gapping: 1142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1212
length of query: 751
length of database: 4,897,600
effective HSP length: 97
effective length of query: 654
effective length of database: 2,136,980
effective search space: 1397584920
effective search space used: 1397584920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0220.1