BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0214.3
(605 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV421890 147 4e-36
BP085186 75 4e-14
BP057603 44 7e-05
BP069078 43 2e-04
TC10152 weakly similar to UP|Q40869 (Q40869) Late embryogenesis ... 32 0.22
TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase ... 32 0.29
TC12711 similar to UP|Q84Y18 (Q84Y18) CAX-interacting protein 4,... 32 0.29
TC19753 similar to UP|Q8H6R5 (Q8H6R5) CTV.12, partial (33%) 32 0.38
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 31 0.50
TC12248 30 1.1
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 30 1.1
TC15626 30 1.1
TC19118 weakly similar to UP|Q84YE8 (Q84YE8) Expressed protein-l... 30 1.5
AV780096 30 1.5
TC13980 similar to UP|Q9SGT0 (Q9SGT0) T6H22.18 protein (F14J16.3... 30 1.5
TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%) 30 1.5
BP034655 29 1.9
BP084855 29 1.9
BP061469 29 1.9
BP040783 29 1.9
>AV421890
Length = 366
Score = 147 bits (372), Expect = 4e-36
Identities = 76/124 (61%), Positives = 99/124 (79%)
Frame = +1
Query: 298 HSGTESNRPQKKKKRNETPEPTKGKDASQPSMEKFMMKNNPQHLAHQSGSSSAPPPTWKS 357
HSGTE++ P K+ ++N+ E KG ++Q SMEKFM+K + + ++ SSSAPP TWK+
Sbjct: 4 HSGTETDPPAKRNRKNKVVETPKG--STQYSMEKFMVKTSSD-MGNKQVSSSAPPTTWKN 174
Query: 358 VLKEFEELTSEEVTSLWDSKIDFNALVETNLVFKADQEKIKRIGLKEACQAIMTKGLEIA 417
LKEF+EL SEE+TSLWDS+I+FNALVETNLVF A +E I +IGL EACQA++TKGLEI+
Sbjct: 175 TLKEFQELISEELTSLWDSRINFNALVETNLVFGAYRENITKIGLPEACQALLTKGLEIS 354
Query: 418 AISK 421
AISK
Sbjct: 355 AISK 366
>BP085186
Length = 388
Score = 74.7 bits (182), Expect = 4e-14
Identities = 43/86 (50%), Positives = 52/86 (60%), Gaps = 20/86 (23%)
Frame = -1
Query: 539 SKAKEQILFLNPQVSINLAGSNPYARIVDGKLINPDTGD--------------------E 578
++A QI FL+P ++INL GSNPYARIV+GKLI+PD D E
Sbjct: 388 AQALNQISFLHPDLAINLEGSNPYARIVNGKLISPDNADGEGNEEEEEEEEEEEEEEEEE 209
Query: 579 EEEEEYEGDKNEEDGNANNNEGEGEN 604
EEE E E +KNEE+ N N EGEGEN
Sbjct: 208 EEEAEPEEEKNEEE-NVNTQEGEGEN 134
>BP057603
Length = 538
Score = 43.9 bits (102), Expect = 7e-05
Identities = 29/84 (34%), Positives = 43/84 (50%)
Frame = -3
Query: 489 KVKEGELVKSQAEITELNSANSELKNENSKLHSEISELKNSILDQLEAGFSKAKEQILFL 548
K+KE E K +T L LK L +E+ ELK + + +GF KA Q+ FL
Sbjct: 470 KIKELEDEKKNL-VTSLEIDRDVLKVTCENLEAEVEELKGEVALKYASGFEKALMQVRFL 294
Query: 549 NPQVSINLAGSNPYARIVDGKLIN 572
P + NL + P+ + DGKL++
Sbjct: 293 YP--NSNLEETGPFKVVKDGKLVD 228
>BP069078
Length = 452
Score = 42.7 bits (99), Expect = 2e-04
Identities = 34/110 (30%), Positives = 55/110 (49%)
Frame = -2
Query: 463 SEKKAADLALENERLKKHAEALSATQKVKEGELVKSQAEITELNSANSELKNENSKLHSE 522
SEK DL + + L+ E + K+KE E K +T L ++ LK +E
Sbjct: 451 SEKDLDDLRKKLDTLE--LENIEKDLKIKELEKEKKNL-VTSLEIDHAVLKVTCENHEAE 281
Query: 523 ISELKNSILDQLEAGFSKAKEQILFLNPQVSINLAGSNPYARIVDGKLIN 572
+ ELK+ + + +GF KA Q+ FL P + NL + P + DGK+++
Sbjct: 280 VEELKSEVAVKYASGFDKAIMQVRFLYP--NSNLEETGPSQVLQDGKVVD 137
>TC10152 weakly similar to UP|Q40869 (Q40869) Late embryogenesis abundant
protein, partial (15%)
Length = 578
Score = 32.3 bits (72), Expect = 0.22
Identities = 30/87 (34%), Positives = 40/87 (45%), Gaps = 3/87 (3%)
Frame = +1
Query: 434 NSAKQLEEKEREIL-KMKATMKLLDSSNKVSEKKAADLALENERLKKHAEALSATQKVKE 492
++A Q+ +K + I K K TM+ S K + KAAD L TQK
Sbjct: 322 DTANQISDKAQNITEKAKQTMQEAWDSTKNNANKAAD------------NVLGKTQK--S 459
Query: 493 GELVKSQAEITE--LNSANSELKNENS 517
E VK AE + +NS N ELK N+
Sbjct: 460 AEYVKENAETVKENMNSKNRELKFSNT 540
>TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase like
protein (At4g14440), partial (75%)
Length = 910
Score = 32.0 bits (71), Expect = 0.29
Identities = 26/88 (29%), Positives = 37/88 (41%)
Frame = -2
Query: 266 LSDDDILRFRREQQAAREKRNPAKTLIDADASHSGTESNRPQKKKKRNETPEPTKGKDAS 325
+SD IL FR +Q E TL+D ++S S + P PT A
Sbjct: 744 ISD*SILTFRS*KQLISELIPKPNTLLDTNSSLS------------LHTLPPPTSSAQAP 601
Query: 326 QPSMEKFMMKNNPQHLAHQSGSSSAPPP 353
P+ ++P+H A S S+PPP
Sbjct: 600 PPTASP-PPPSSPRHRAPSQQSPSSPPP 520
>TC12711 similar to UP|Q84Y18 (Q84Y18) CAX-interacting protein 4, partial
(5%)
Length = 500
Score = 32.0 bits (71), Expect = 0.29
Identities = 20/67 (29%), Positives = 33/67 (48%), Gaps = 3/67 (4%)
Frame = +2
Query: 267 SDDDILRFRREQQAAREKRNPAKTLIDADASHSGTESNRPQKKKKRNETPE---PTKGKD 323
SDDD RR +++ +EKR D+D+ S S R ++K +R ++ + G +
Sbjct: 74 SDDDDEYTRRHRKSRKEKRRRRSRRSDSDSETSDDSSTRHKRKSQREKSSSSDTDSSGYN 253
Query: 324 ASQPSME 330
SQ E
Sbjct: 254 DSQDGQE 274
>TC19753 similar to UP|Q8H6R5 (Q8H6R5) CTV.12, partial (33%)
Length = 690
Score = 31.6 bits (70), Expect = 0.38
Identities = 23/84 (27%), Positives = 35/84 (41%)
Frame = +2
Query: 269 DDILRFRREQQAAREKRNPAKTLIDADASHSGTESNRPQKKKKRNETPEPTKGKDASQPS 328
D +L+ A + R + + + A+ +G N N PE TK K QPS
Sbjct: 179 DVVLKSESNWDAHQVSRKHREAISNLKANAAGLTQNN-------NAKPEITKAKP-EQPS 334
Query: 329 MEKFMMKNNPQHLAHQSGSSSAPP 352
+ +F M + Q + SS PP
Sbjct: 335 VSQFKMPQSSQEVPKPQSSSVLPP 406
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 31.2 bits (69), Expect = 0.50
Identities = 11/35 (31%), Positives = 25/35 (71%)
Frame = +3
Query: 569 KLINPDTGDEEEEEEYEGDKNEEDGNANNNEGEGE 603
K + D +EE+++E E D++E+D + ++++ +GE
Sbjct: 231 KPVQTDNEEEEDDDEEEDDEDEDDDDDDDDDDDGE 335
>TC12248
Length = 591
Score = 30.0 bits (66), Expect = 1.1
Identities = 20/70 (28%), Positives = 30/70 (42%)
Frame = +3
Query: 267 SDDDILRFRREQQAAREKRNPAKTLIDADASHSGTESNRPQKKKKRNETPEPTKGKDASQ 326
S + FR E +++ +L+ A S S T S P +KKKRN+ P +
Sbjct: 372 SSASLFGFREEDNQMNQQQQ--HSLVAAAPSSSATPSAPPPQKKKRNQPGTPNPDAEVIA 545
Query: 327 PSMEKFMMKN 336
S + M N
Sbjct: 546 LSPKTLMATN 575
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 30.0 bits (66), Expect = 1.1
Identities = 13/34 (38%), Positives = 24/34 (70%), Gaps = 3/34 (8%)
Frame = +2
Query: 574 DTGDEEEEEEYEGDKNEE---DGNANNNEGEGEN 604
+ GDE+E+E+ + D+ EE D + ++ EGEG++
Sbjct: 785 EDGDEDEDEDDDDDEEEEDDDDDDEDDEEGEGKS 886
Score = 27.7 bits (60), Expect = 5.5
Identities = 10/29 (34%), Positives = 18/29 (61%)
Frame = +2
Query: 574 DTGDEEEEEEYEGDKNEEDGNANNNEGEG 602
D DEEEE++ + D+++E+G + G
Sbjct: 815 DDDDEEEEDDDDDDEDDEEGEGKSKSKSG 901
>TC15626
Length = 543
Score = 30.0 bits (66), Expect = 1.1
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +2
Query: 340 HLAHQSGSSSAPPPTWKSVLKEFEELTSEEVTSLWDSKIDFNALVETNLVFK 391
H Q +APP ++ + ++L S + S+W+S D N+LV ++V K
Sbjct: 137 HRGLQVTEGAAPPLLVDLIVTQMKDLISSGMRSVWESCGDSNSLVVDSIVRK 292
>TC19118 weakly similar to UP|Q84YE8 (Q84YE8) Expressed protein-like
protein, partial (11%)
Length = 932
Score = 29.6 bits (65), Expect = 1.5
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 5/103 (4%)
Frame = +3
Query: 415 EIAAISKMVDLETAGFDGINSAKQLEEKEREILKMKATMKLLDSSNKVSEKKAADLALEN 474
E+A + D+ + FD A+ EEK+RE + + +D ++ VSE+ D + N
Sbjct: 165 ELANPPTLTDIFHSKFD---KAETKEEKQREGKSKQGVKRKIDHNDVVSEENVVDPQVGN 335
Query: 475 -ERLK----KHAEALSATQKVKEGELVKSQAEITELNSANSEL 512
++L+ K A+ L+ + K L + EL S S+L
Sbjct: 336 GQKLQDGNFKKAKNLAVSMATKAASLAR------ELKSIKSDL 446
>AV780096
Length = 564
Score = 29.6 bits (65), Expect = 1.5
Identities = 39/171 (22%), Positives = 77/171 (44%), Gaps = 5/171 (2%)
Frame = +1
Query: 431 DGINSAKQLEEKEREILKMKATMKLLDSSNKVSEKKAADLALENERLKKHAE-ALSATQK 489
+G +S ++ EE++ E + + ++ ++ E A + ++E + E A++A
Sbjct: 40 NGSDSEEEEEEQQEEEVHDQ-----IEEDDEELEAVARSASSDDEPDESGGEDAVAADDD 204
Query: 490 VKEGELVKSQAEITELNSAN-SELKNENSKLHSEISELKNSILDQLEAGFSKAKEQILFL 548
++ E + EI++ A EL+ + EI + +N+ +D K + L
Sbjct: 205 NQDDEGNAADPEISKREKARLKELQQLKKQKIQEILDAQNAAIDADMNNRGKGRLNYLLQ 384
Query: 549 NPQVSINLAGSNPYARIVDGKLINPDTG---DEEEEEEYEGDKNEEDGNAN 596
++ +LA ++ + GK +EEE+EEY K EE G AN
Sbjct: 385 QTELFAHLAKADQSSSQKKGKGRGRHASKVTEEEEDEEYL--KEEEGGTAN 531
>TC13980 similar to UP|Q9SGT0 (Q9SGT0) T6H22.18 protein (F14J16.33), partial
(12%)
Length = 565
Score = 29.6 bits (65), Expect = 1.5
Identities = 20/67 (29%), Positives = 26/67 (37%)
Frame = +2
Query: 287 PAKTLIDADASHSGTESNRPQKKKKRNETPEPTKGKDASQPSMEKFMMKNNPQHLAHQSG 346
PA T A + S N P ++TP P +S P+ +NP H Q
Sbjct: 242 PASTTPSATRASSLGIPN-PPTSSSASKTPSPCSPPTSSSPTGSSSHSTSNPPHPPPQQQ 418
Query: 347 SSSAPPP 353
S PPP
Sbjct: 419 PFSPPPP 439
>TC18665 similar to UP|O23061 (O23061) BAC IG005I10, partial (65%)
Length = 451
Score = 29.6 bits (65), Expect = 1.5
Identities = 18/62 (29%), Positives = 31/62 (49%)
Frame = -2
Query: 543 EQILFLNPQVSINLAGSNPYARIVDGKLINPDTGDEEEEEEYEGDKNEEDGNANNNEGEG 602
+Q+ L P + G N +D GDE+E+ + +G ++EE G+ + EGE
Sbjct: 300 KQLKALPPHIRRIKPGKNGA*DELDVADFESGEGDEDEDLDSDGFRDEEIGDDDEGEGEE 121
Query: 603 EN 604
E+
Sbjct: 120 ED 115
>BP034655
Length = 517
Score = 29.3 bits (64), Expect = 1.9
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +1
Query: 577 DEEEEEEYEGDKNEEDGNANNNEGEGENH 605
+EEEEEE E ++ EE+ ++ E E E++
Sbjct: 172 EEEEEEEEEEEEEEEEDEEDDEEDEDEDY 258
Score = 28.5 bits (62), Expect = 3.2
Identities = 20/63 (31%), Positives = 28/63 (43%), Gaps = 4/63 (6%)
Frame = +1
Query: 546 LFLNPQVSINLAGSNPYARI----VDGKLINPDTGDEEEEEEYEGDKNEEDGNANNNEGE 601
LF P S + N A D L + D +EEEEEE E ++ EE+ E E
Sbjct: 46 LFATPMASPSEFHRNEIAAAHLTAADFGLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDE 225
Query: 602 GEN 604
++
Sbjct: 226 EDD 234
>BP084855
Length = 373
Score = 29.3 bits (64), Expect = 1.9
Identities = 20/67 (29%), Positives = 30/67 (43%), Gaps = 6/67 (8%)
Frame = -2
Query: 544 QILFLNPQVSINLAGSNPYARIVDGKLINPDTGDEE------EEEEYEGDKNEEDGNANN 597
QI + P ++ G I DG+++ D D + E EYE +K E+ +
Sbjct: 372 QISVVEPDFDLSCIGF--LKEIKDGQVVGDDDVDIDLLPQFNSESEYEEEKCEDGHGEDK 199
Query: 598 NEGEGEN 604
N EGEN
Sbjct: 198 NGDEGEN 178
>BP061469
Length = 482
Score = 29.3 bits (64), Expect = 1.9
Identities = 15/69 (21%), Positives = 29/69 (41%)
Frame = +1
Query: 284 KRNPAKTLIDADASHSGTESNRPQKKKKRNETPEPTKGKDASQPSMEKFMMKNNPQHLAH 343
+ NP+ L A ++ + +P P + A +P+ +N P+ LA
Sbjct: 4 QENPSHFLFPHAALYNSSNPESDSSSVNTTGSPPPACRESAQRPASSSRSHRNQPRRLAA 183
Query: 344 QSGSSSAPP 352
++ S+PP
Sbjct: 184 RNPRPSSPP 210
>BP040783
Length = 339
Score = 29.3 bits (64), Expect = 1.9
Identities = 17/59 (28%), Positives = 26/59 (43%)
Frame = -1
Query: 301 TESNRPQKKKKRNETPEPTKGKDASQPSMEKFMMKNNPQHLAHQSGSSSAPPPTWKSVL 359
T SN P + + S S++ ++ P HL+ S SS+ PPPT S +
Sbjct: 222 T*SNYPSHIFPFTSSSSSSLSSSTSPSSIKPSSLQPPPPHLSSSSSSSNPPPPTIPSTI 46
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.130 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,556,894
Number of Sequences: 28460
Number of extensions: 139609
Number of successful extensions: 1053
Number of sequences better than 10.0: 74
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 981
length of query: 605
length of database: 4,897,600
effective HSP length: 96
effective length of query: 509
effective length of database: 2,165,440
effective search space: 1102208960
effective search space used: 1102208960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0214.3